scholarly journals Reversibility of the oleanolic acid monoglycosides transport across the tonoplast in vacuoles isolated from Calendula officinalis leaves.

1997 ◽  
Vol 44 (1) ◽  
pp. 55-59
Author(s):  
A Szakiel ◽  
W Janiszowska
Keyword(s):  
Oleanolic Acid ◽  
Calendula Officinalis ◽  
Leaf Protoplasts

The possibility of the reversible tonoplast transport of oleanolic acid monoglycosides was investigated in vacuoles isolated from Calendula officinalis leaf protoplasts. The obtained results point to the reversibility of the transport of monoglucoside I, whereas monoglucuronide F seems to be definitely stored in the vacuolar space.

scholarly journals The effect of inorganic pyrophosphate on the transport of oleanolic acid monoglycosides into vacuoles isolated from Calendula officinalis leaves.

1998 ◽  
Vol 45 (3) ◽  
pp. 819-823
Author(s):  
A Szakiel ◽  
W Janiszowska
Keyword(s):  
Active Transport ◽  
Oleanolic Acid ◽  
Proton Pumps ◽  
Calendula Officinalis ◽  
Inorganic Pyrophosphate ◽  
Leaf Protoplasts

The influence of exogenous inorganic pyrophosphate on the transport of oleanolic acid monoglucoside and monoglucuronide to vacuoles isolated from Calendula officinalis leaf protoplasts was studied. The results indicate that the transport of both monoglycosides is carrier-mediated; however, the transport of the monoglucuronide is passive, and that of the monoglucoside active. The active transport of the monoglucoside is dependent on tonoplast energization created as a result of cooperation of two vacuolar proton pumps: H+-ATPase and H+-PPase.

scholarly journals Specificity of the tonoplast transport of the oleanolic acid monoglycosides in the vacuoles from Calendula officinalis leaves.

1997 ◽  
Vol 44 (2) ◽  
pp. 333-338
Author(s):  
A Szakiel ◽  
W Janiszowska
Keyword(s):  
Oleanolic Acid ◽  
Calendula Officinalis ◽  
Sugar Moiety ◽  
Oleanolic Acid Glycosides ◽  
Leaf Protoplasts

The specificity of two separate tonoplast permeases transporting oleanolic acid glycosides was investigated in vacuoles isolated from leaf protoplasts of marigold (Calendula officinalis) with the use of chemically synthesized analogs. The results indicate that the proper structure of both parts of oleanolic acid monoglycoside, i.e. aglycon and the sugar moiety, are required for binding to a specific tonoplast carrier.

The structure of glycosides of oleanolic acid isolated from the roots of Calendula officinalis

1971 ◽  
Vol 10 (5) ◽  
pp. 1121-1124 ◽  
Author(s):  
Zdzislaw Wojciechowski ◽  
Anna Jelonkiewicz-Konador ◽  
Marek Tomaszewski ◽  
Jacek Jankowski ◽  
Zofia Kasprzyk
Keyword(s):  
Oleanolic Acid ◽  
Calendula Officinalis

Biosynthesis of oleanolic acid glycosides in protoplasts isolated from Calendula officinalis L. roots

2006 ◽  
Vol 28 (3) ◽  
pp. 217-223 ◽  
Author(s):  
Dariusz Ruszkowski ◽  
Anna Szakiel ◽  
Elbieta Auguciska ◽  
Wirginia Janiszowska
Keyword(s):  
Oleanolic Acid ◽  
Calendula Officinalis ◽  
Oleanolic Acid Glycosides ◽  
Calendula Officinalis L

Oleanolic acid 3-O-β-D-glucuronopyranoside from the roots of Calendula officinalis

1973 ◽  
Vol 9 (4) ◽  
pp. 530-531
Author(s):  
L. P. Vecherko ◽  
É. P. Zinkevich ◽  
Leonid M. Kogan
Keyword(s):  
Oleanolic Acid ◽  
Calendula Officinalis

The Transport of Oleanolic Acid in Calendula officinalis

1968 ◽  
Vol 21 (5) ◽  
pp. 966-970 ◽  
Author(s):  
Zofia Kasprzyk ◽  
Zdzislaw Wojciechowski ◽  
Krystyna Czerniakowska
Keyword(s):  
Oleanolic Acid ◽  
Calendula Officinalis

Biosynthesis of oleanolic acid glycosides in isolated ligulate flowers of calendula officinalis

1983 ◽  
Vol 22 (1) ◽  
pp. 111-113 ◽  
Author(s):  
Wiesa̵wa Szyja ◽  
Bogusa̵w Wik̵omirski ◽  
Zofia Kasprzyk
Keyword(s):  
Oleanolic Acid ◽  
Calendula Officinalis ◽  
Oleanolic Acid Glycosides

scholarly journals Alterations in oleanolic acid and sterol content in marigold (Calendula officinalis) hairy root cultures in response to stimulation by selected phytohormones

2021 ◽  
Vol 43 (3) ◽  
Author(s):  
Abdulwadood S. M. Alsoufi ◽  
Klaudia Staśkiewicz ◽  
Michał Markowski
Keyword(s):  
Oleanolic Acid ◽  
Hairy Root ◽  
Hairy Roots ◽  
Naphthaleneacetic Acid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Calendula Officinalis ◽  
Plant Metabolites ◽  
Triterpenoid Saponins

AbstractHairy root cultures are an efficient tool for the biotechnological production of plant metabolites and a convenient experimental model for analyzing the effect of various compounds on plant metabolism. In contrast to many other types of in vitro plant cultures, hairy roots do not require an external supply of phytohormones to the medium. Consequently, plant growth regulators such as auxins and cytokinins are rarely used as elicitors in hairy root in vitro cultures; however, they can strongly influence plant defense responses. The aim of this study was to investigate the influence of two auxins: natural indole-3-acetic acid (IAA) and synthetic 1-naphthaleneacetic acid (NAA), as well as two cytokinins: natural kinetin and synthetic 6-benzylaminopurine (BAP) at a concentration of 0.75 mg/L on the metabolism of sterols and triterpenoids in Calendula officinalis hairy roots. Auxins prevented the accumulation of triterpenoid saponins (oleanolic acid glycosides), while cytokinin BAP increased their accumulation by 17% and their release into the culture medium by a factor of 10. Other cytokinins and kinetins increased the sterol levels by 17%, the level of stigmasterol by 15%, and the level of isofucosterol by 7 times.

Sign in / Sign up

Export Citation Format

Share Document