scholarly journals Specificity of the tonoplast transport of the oleanolic acid monoglycosides in the vacuoles from Calendula officinalis leaves.

1997 ◽  
Vol 44 (2) ◽  
pp. 333-338
Author(s):  
A Szakiel ◽  
W Janiszowska
Keyword(s):  
Oleanolic Acid ◽  
Calendula Officinalis ◽  
Sugar Moiety ◽  
Oleanolic Acid Glycosides ◽  
Leaf Protoplasts

The specificity of two separate tonoplast permeases transporting oleanolic acid glycosides was investigated in vacuoles isolated from leaf protoplasts of marigold (Calendula officinalis) with the use of chemically synthesized analogs. The results indicate that the proper structure of both parts of oleanolic acid monoglycoside, i.e. aglycon and the sugar moiety, are required for binding to a specific tonoplast carrier.

scholarly journals The effect of inorganic pyrophosphate on the transport of oleanolic acid monoglycosides into vacuoles isolated from Calendula officinalis leaves.

1998 ◽  
Vol 45 (3) ◽  
pp. 819-823
Author(s):  
A Szakiel ◽  
W Janiszowska
Keyword(s):  
Active Transport ◽  
Oleanolic Acid ◽  
Proton Pumps ◽  
Calendula Officinalis ◽  
Inorganic Pyrophosphate ◽  
Leaf Protoplasts

The influence of exogenous inorganic pyrophosphate on the transport of oleanolic acid monoglucoside and monoglucuronide to vacuoles isolated from Calendula officinalis leaf protoplasts was studied. The results indicate that the transport of both monoglycosides is carrier-mediated; however, the transport of the monoglucuronide is passive, and that of the monoglucoside active. The active transport of the monoglucoside is dependent on tonoplast energization created as a result of cooperation of two vacuolar proton pumps: H+-ATPase and H+-PPase.

Biosynthesis of oleanolic acid glycosides in protoplasts isolated from Calendula officinalis L. roots

2006 ◽  
Vol 28 (3) ◽  
pp. 217-223 ◽  
Author(s):  
Dariusz Ruszkowski ◽  
Anna Szakiel ◽  
Elbieta Auguciska ◽  
Wirginia Janiszowska
Keyword(s):  
Oleanolic Acid ◽  
Calendula Officinalis ◽  
Oleanolic Acid Glycosides ◽  
Calendula Officinalis L

Biosynthesis of oleanolic acid glycosides in isolated ligulate flowers of calendula officinalis

1983 ◽  
Vol 22 (1) ◽  
pp. 111-113 ◽  
Author(s):  
Wiesa̵wa Szyja ◽  
Bogusa̵w Wik̵omirski ◽  
Zofia Kasprzyk
Keyword(s):  
Oleanolic Acid ◽  
Calendula Officinalis ◽  
Oleanolic Acid Glycosides

Biosynthesis of oleanolic acid glycosides in calendula officinalis inflorescences

1983 ◽  
Vol 22 (1) ◽  
pp. 107-109 ◽  
Author(s):  
Paweł Ciborowski ◽  
Bogusław Wiłkomirski ◽  
Wojciech Zdrojewski ◽  
Zofia Kasprzyk
Keyword(s):  
Oleanolic Acid ◽  
Calendula Officinalis ◽  
Oleanolic Acid Glycosides

scholarly journals Reversibility of the oleanolic acid monoglycosides transport across the tonoplast in vacuoles isolated from Calendula officinalis leaves.

1997 ◽  
Vol 44 (1) ◽  
pp. 55-59
Author(s):  
A Szakiel ◽  
W Janiszowska
Keyword(s):  
Oleanolic Acid ◽  
Calendula Officinalis ◽  
Leaf Protoplasts

The possibility of the reversible tonoplast transport of oleanolic acid monoglycosides was investigated in vacuoles isolated from Calendula officinalis leaf protoplasts. The obtained results point to the reversibility of the transport of monoglucoside I, whereas monoglucuronide F seems to be definitely stored in the vacuolar space.

scholarly journals Production of oleanolic acid glycosides by hairy root established cultures of Calendula officinalis L.

2013 ◽  
Vol 60 (3) ◽  
Author(s):  
Marek Długosz ◽  
Ewa Wiktorowska ◽  
Anita Wiśniewska ◽  
Cezary Pączkowski
Keyword(s):  
Oleanolic Acid ◽  
Hairy Root ◽  
Root Culture ◽  
Dry Weight ◽  
Hairy Root Culture ◽  
Calendula Officinalis ◽  
Kinase Gene ◽  
High Positive Correlation ◽  
Oleanolic Acid Glycosides ◽  
Calendula Officinalis L

In order to initiate hairy root culture initiation cotyledons and hypocotyls of Calendula officinalis L. were infected with Agrobacterium rhizogenes strain ATCC 15834 or the same strain containing pCAMBIA 1381Z vector with β-glucuronidase reporter gene under control of promoter of NIK (Nematode Induced Kinase) gene. The efficiency of induction of hairy roots reached 33.8% for cotyledons and 66.6% for hypocotyls together for both transformation experiments. Finally, eight control and nine modified lines were established as a long-term culture. The hairy root cultures showed the ability to synthesize oleanolic acid mainly (97%) as glycosides; control lines contained it at the average 8.42 mg · g(-1) dry weight in tissue and 0.23 mg · dm(-3) in medium; modified lines: 4.59 mg · g(-1) for the tissue, and 0.48 mg · dm(-3) for the medium. Additionally lines showed high positive correlation between dry/fresh weight and oleanolic acid concentration in tissue. Using the Killiani mixture in acidic hydrolysis of oleanolic acid glycosides released free aglycones that were partially acetylated in such conditions.

scholarly journals Metabolism of [3-3H]oleanolic acid in the isolated Calendula officinalis leaf cells and transport of the synthesized glycosides to the cell wall and the extracellular space.

1995 ◽  
Vol 42 (1) ◽  
pp. 25-29 ◽  
Author(s):  
A Szakiel ◽  
I Wasiukiewicz ◽  
W Janiszowska
Keyword(s):  
Cell Wall ◽  
Oleanolic Acid ◽  
Extracellular Space ◽  
Cell Walls ◽  
Calendula Officinalis ◽  
Oleanolic Acid Glycosides ◽  
First Time

It has been shown for the first time that [3-3H]oleanolic acid glycosides formed in the cytosol of C. officinalis leaf cells are transported to the extracellular space in the form of pentaglucoside VI (44%), whereas glucuronides derived from [3-3H]oleanolic acid 3-O-monoglucuronide (29%) as well as a part of glucosides (24%) were transported into the cell walls.

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