scholarly journals Isolation, Pure Cultivation and Total DNA Extraction of Microcystis aeruginosa Kutz in Dianchi Lake

2001 ◽  
Vol 13 (3) ◽  
pp. 285-288 ◽  
Author(s):  
LU Yuan ◽  
◽  
WEN Jianfan ◽  
LV Tianwen
Keyword(s):  
Dna Extraction ◽  
Microcystis Aeruginosa ◽  
Dianchi Lake ◽  
Total Dna

scholarly journals A fast and efficient method for total DNA extraction from soil filamentous fungi

2014 ◽  
Vol 32 (2) ◽  
pp. 75-78 ◽  
Author(s):  
Wilson Huanca-Mamani ◽  
Ricardo Salvatierra Martínez ◽  
Germán Sepúlveda-Chavera
Keyword(s):  
Dna Extraction ◽  
Filamentous Fungi ◽  
Efficient Method ◽  
Total Dna

Plant Total DNA Extraction

1998 ◽  
pp. 10-14 ◽  
Author(s):  
Julia Rueda ◽  
Rosario Linacero ◽  
Ana M. Vázquez
Keyword(s):  
Dna Extraction ◽  
Total Dna

scholarly journals Small-scale total DNA extraction from bacteria and yeast for PCR applications

2005 ◽  
Vol 347 (2) ◽  
pp. 333-335 ◽  
Author(s):  
José Luis Ruiz-Barba ◽  
Antonio Maldonado ◽  
Rufino Jiménez-Díaz
Keyword(s):  
Dna Extraction ◽  
Small Scale ◽  
Total Dna

scholarly journals Detection of Badnavirus in pineapple in northeastern Brazil

2020 ◽  
Vol 5 (4) ◽  
pp. 2450-2463
Author(s):  
Edlene Maria da Silva Moraes Santos ◽  
Jaqueline Figueredo de Oliveira Costa ◽  
Mayra Machado de Medeiros Ferro ◽  
Sarah Jacqueline Cavalcanti da Silva ◽  
Iraildes Pereira Assunção ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Molecular Analysis ◽  
Distinct Species ◽  
Northeastern Brazil ◽  
Ananas Comosus ◽  
Pairwise Comparisons ◽  
Sequencing Analysis ◽  
First Report ◽  
Northeastern Region ◽  
Total Dna

ABSTRACT: Brazil is one of the main global producers of pineapple (Ananas comosus L. Merril), with emphasis in the Northeastern region of the country. Pineapple is exposed to several phytosanitary problems, including viruses. Pineapple badnaviruses are caused by two distinct species: Pineapple bacilliform CO virus (PBCoV) and Pineapple bacilliform ER virus (PBErV). The present study aimed to detect possible species of the genus Badnavirus in pineapple samples in Northeastern Brazil, via PCR and sequencing of the RT/RNaseH region. Leaf samples of pineapples were collected in the states of Alagoas, Maranhão, Paraiba and Pernambuco, and subsequently subjected to total DNA extraction and amplification via PCR. Badnavirus positive samples were selected for sequencing. Analysis of pairwise comparisons revealed that all sequences obtained in this work showed an identity greater than 80% with the sequence of the species PBCoV (EU377664), from Australia, fully corroborating with phylogenetic analyzes. These results suggest the widespread of PBCoV in Northeastern Brazil and record the first report of Badnavirus in pineapple culture in Brazil. KEYWORDS: Caulimoviridae, Ananas comosus, molecular analysis.

Algicidal characterization and mechanism of Bacillus licheniformis Sp34 against Microcystis aeruginosa in Dianchi Lake

2019 ◽  
Vol 59 (11) ◽  
pp. 1112-1124 ◽  
Author(s):  
Jinyu Liu ◽  
Caiyun Yang ◽  
Yuxin Chi ◽  
Donghao Wu ◽  
Xianzhu Dai ◽  
...  
Keyword(s):  
Microcystis Aeruginosa ◽  
Bacillus Licheniformis ◽  
Dianchi Lake

Fast and easy method for total DNA extraction and gene amplification from larvae, spat and adult mussels Mytilus trossulus from the Baltic Sea

2013 ◽  
Vol 42 (4) ◽  
Author(s):  
Rafał Lasota ◽  
Joanna Piłczyńska ◽  
Suzanne Williams ◽  
Maciej Wołowicz
Keyword(s):  
Dna Extraction ◽  
Gene Amplification ◽  
Nuclear Dna ◽  
Mytilus Trossulus ◽  
Easy Method ◽  
Time Saving ◽  
Phenol Extraction ◽  
Total Dna ◽  
The Baltic ◽  
Mussel Mytilus Trossulus

AbstractThree nuclear DNA markers that diagnostically differentiate mussels within the Mytilus edulis complex (M. edulis, M. trossulus and M. galloprovincialis) are commonly used in taxonomic investigations: Glu5’, ITS and EFbis. As a rule, DNA extraction is performed before amplification. It is a time consuming process in the case of traditional methods based on chloroform and phenol extraction or relatively expensive using kits with ready spin columns. Moreover, DNA isolation from larvae is problematic, because of the small amount of tissue available. In this report we describe a simple, fast and inexpensive method of DNA extraction and gene amplification from larvae, spat and adults of the Baltic mussel Mytilus trossulus. The extraction method is adapted from that of Wang et al. (2006) and is based on digestion of tissue or whole animals in STE solution and direct gene amplification. On the basis of the results of routine analyses of mussels carried out in our laboratory we have concluded that the method we propose gives results that are consistent with standard methods, without requiring expensive reagents/equipment and is time saving.

Nickel Induced Oxidative Stress and the Responses of SOD Isoenzymes in Microcystis aeruginosa FACHB-905

2011 ◽  
Vol 356-360 ◽  
pp. 119-126
Author(s):  
Li Juan Huang ◽  
Xue Xiu Chang ◽  
Cheng Wu
Keyword(s):  
Oxidative Stress ◽  
Microcystis Aeruginosa ◽  
Culture Medium ◽  
Dianchi Lake ◽  
Sod Activity ◽  
Oxygen Stress ◽  
Long Period ◽  
South West ◽  
Unfavorable Condition ◽  
Test Cultures

The laboratory experiment was conducted to investigated the effect of nickel over a concentration gradient of 0.1~1.00 mg/L on biomass ( indicated by absorbance of cell culture at 663nm wavelength ), superoxide anion (O2•ˉ), malondialdehyde (MDA), and superoxide dismutase (SOD) in the cyanobacterium Microcystis aeruginosa FACHB 905 isolated from Dianchi Lake, south west of China . The results showed: (1) M. aeruginosa could live normally in Ni (II)-absence culture medium，but 0.3 mg/L Ni(II) was the fittest concentration for test M. aeruginosa, in which biomass and SOD activity were highest among all test cultures; (2) Ni(II) induced O2•ˉ and MDA in M. aeruginosa cells under the experimental condition, showing that Ni(II) could influence on M. aeruginosa by inducing oxidative stress; (3) Fe-SOD and Mn-SOD were found in M. aeruginosa and both of them were induced by lower Ni(II) but inhibited by higher concentration. The multiformity of SOD isoenzymes enhance the resistance of M. aeruginosa to oxygen stress induced by unfavorable condition, which explained that M. aeruginosa is the preponderant species in badly polluted Dianchi Lake water for long period.

scholarly journals Evaluation of Five Methods for Total DNA Extraction from Western Corn Rootworm Beetles

PLoS ONE ◽  
2010 ◽  
Vol 5 (8) ◽  
pp. e11963 ◽  
Author(s):  
Hong Chen ◽  
Murugesan Rangasamy ◽  
Sek Yee Tan ◽  
Haichuan Wang ◽  
Blair D. Siegfried
Keyword(s):  
Dna Extraction ◽  
Western Corn Rootworm ◽  
Corn Rootworm ◽  
Total Dna

scholarly journals Comparison of Total DNA Extraction Methods for Microbial Community Form Polluted Soil

2015 ◽  
Vol 6 ◽  
pp. 616-622 ◽  
Author(s):  
Ana-Maria Tanase ◽  
Ioana Mereuta ◽  
Iulia Chiciudean ◽  
Robertina Ionescu ◽  
Ligia Milea ◽  
...  
Keyword(s):  
Microbial Community ◽  
Dna Extraction ◽  
Extraction Methods ◽  
Polluted Soil ◽  
Total Dna ◽  
Dna Extraction Methods
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