scholarly journals Small-scale total DNA extraction from bacteria and yeast for PCR applications

2005 ◽  
Vol 347 (2) ◽  
pp. 333-335 ◽  
Author(s):  
José Luis Ruiz-Barba ◽  
Antonio Maldonado ◽  
Rufino Jiménez-Díaz
Keyword(s):  
Dna Extraction ◽  
Small Scale ◽  
Total Dna

scholarly journals A fast and efficient method for total DNA extraction from soil filamentous fungi

2014 ◽  
Vol 32 (2) ◽  
pp. 75-78 ◽  
Author(s):  
Wilson Huanca-Mamani ◽  
Ricardo Salvatierra Martínez ◽  
Germán Sepúlveda-Chavera
Keyword(s):  
Dna Extraction ◽  
Filamentous Fungi ◽  
Efficient Method ◽  
Total Dna

Plant Total DNA Extraction

1998 ◽  
pp. 10-14 ◽  
Author(s):  
Julia Rueda ◽  
Rosario Linacero ◽  
Ana M. Vázquez
Keyword(s):  
Dna Extraction ◽  
Total Dna

scholarly journals Isolation, Pure Cultivation and Total DNA Extraction of Microcystis aeruginosa Kutz in Dianchi Lake

2001 ◽  
Vol 13 (3) ◽  
pp. 285-288 ◽  
Author(s):  
LU Yuan ◽  
◽  
WEN Jianfan ◽  
LV Tianwen
Keyword(s):  
Dna Extraction ◽  
Microcystis Aeruginosa ◽  
Dianchi Lake ◽  
Total Dna

scholarly journals Detection of Badnavirus in pineapple in northeastern Brazil

2020 ◽  
Vol 5 (4) ◽  
pp. 2450-2463
Author(s):  
Edlene Maria da Silva Moraes Santos ◽  
Jaqueline Figueredo de Oliveira Costa ◽  
Mayra Machado de Medeiros Ferro ◽  
Sarah Jacqueline Cavalcanti da Silva ◽  
Iraildes Pereira Assunção ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Molecular Analysis ◽  
Distinct Species ◽  
Northeastern Brazil ◽  
Ananas Comosus ◽  
Pairwise Comparisons ◽  
Sequencing Analysis ◽  
First Report ◽  
Northeastern Region ◽  
Total Dna

ABSTRACT: Brazil is one of the main global producers of pineapple (Ananas comosus L. Merril), with emphasis in the Northeastern region of the country. Pineapple is exposed to several phytosanitary problems, including viruses. Pineapple badnaviruses are caused by two distinct species: Pineapple bacilliform CO virus (PBCoV) and Pineapple bacilliform ER virus (PBErV). The present study aimed to detect possible species of the genus Badnavirus in pineapple samples in Northeastern Brazil, via PCR and sequencing of the RT/RNaseH region. Leaf samples of pineapples were collected in the states of Alagoas, Maranhão, Paraiba and Pernambuco, and subsequently subjected to total DNA extraction and amplification via PCR. Badnavirus positive samples were selected for sequencing. Analysis of pairwise comparisons revealed that all sequences obtained in this work showed an identity greater than 80% with the sequence of the species PBCoV (EU377664), from Australia, fully corroborating with phylogenetic analyzes. These results suggest the widespread of PBCoV in Northeastern Brazil and record the first report of Badnavirus in pineapple culture in Brazil. KEYWORDS: Caulimoviridae, Ananas comosus, molecular analysis.

A rapid and simple method for small-scale DNA extraction inAgavaceae and other tropical plants

2002 ◽  
Vol 20 (3) ◽  
pp. 299-299 ◽  
Author(s):  
Miguel Keb-Llanes ◽  
Gerardo González ◽  
Bartolomé Chi-Manzanero ◽  
Diógenes Infante
Keyword(s):  
Dna Extraction ◽  
Small Scale ◽  
Simple Method ◽  
Tropical Plants

Fast and easy method for total DNA extraction and gene amplification from larvae, spat and adult mussels Mytilus trossulus from the Baltic Sea

2013 ◽  
Vol 42 (4) ◽  
Author(s):  
Rafał Lasota ◽  
Joanna Piłczyńska ◽  
Suzanne Williams ◽  
Maciej Wołowicz
Keyword(s):  
Dna Extraction ◽  
Gene Amplification ◽  
Nuclear Dna ◽  
Mytilus Trossulus ◽  
Easy Method ◽  
Time Saving ◽  
Phenol Extraction ◽  
Total Dna ◽  
The Baltic ◽  
Mussel Mytilus Trossulus

AbstractThree nuclear DNA markers that diagnostically differentiate mussels within the Mytilus edulis complex (M. edulis, M. trossulus and M. galloprovincialis) are commonly used in taxonomic investigations: Glu5’, ITS and EFbis. As a rule, DNA extraction is performed before amplification. It is a time consuming process in the case of traditional methods based on chloroform and phenol extraction or relatively expensive using kits with ready spin columns. Moreover, DNA isolation from larvae is problematic, because of the small amount of tissue available. In this report we describe a simple, fast and inexpensive method of DNA extraction and gene amplification from larvae, spat and adults of the Baltic mussel Mytilus trossulus. The extraction method is adapted from that of Wang et al. (2006) and is based on digestion of tissue or whole animals in STE solution and direct gene amplification. On the basis of the results of routine analyses of mussels carried out in our laboratory we have concluded that the method we propose gives results that are consistent with standard methods, without requiring expensive reagents/equipment and is time saving.

DETECTION OF PHYTOPLASMAS ASSOCIATED WITH KALIMANTAN WILT DISEASE OF COCONUT BY THE POLYMERASE CHAIN REACTION

2020 ◽  
Vol 12 (4) ◽  
pp. 154 ◽  
Author(s):  
J.S. WAROKKA J.S. WAROKKA ◽  
P. JONES P. JONES ◽  
M.J. DICKINSON M.J. DICKINSON
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Nested Pcr ◽  
Cocos Nucifera ◽  
Pcr Analysis ◽  
Small Scale ◽  
Chain Reaction ◽  
Pests And Diseases ◽  
Polymerase Chain ◽  
Cocos Nucifera L

ABSTRACT<br />Coconut is the second Indonesia’s most important social commodity<br />after rice. There are more than 3.6 million hectares of coconut plantations<br />in Indonesia equivalent to one third of the total world coconut area.<br />However, the production and productivity of the coconut are very low and<br />unstable for various reasons, including pests and diseases. Kalimantan wilt<br />(KW) disease causes extensive damage to coconut plantation. In previous<br />investigations, bacteria, fungi, viruses, viroids and soil-borne pathogens<br />such as nematodes were tested, but none of them were consistently<br />associated with the disease. The objective of this research was to detect<br />and diagnose the phytoplasma associating with KW. Two DNA extraction<br />methods, namely a modification of CTAB method involving grinding<br />coconut trunk tissue in pre-warmed CTAB instead of liquid nitrogen, and a<br />small scale DNA extraction method, were used to prepare DNA from<br />coconut trunk tissues. Research results showed that both methods were<br />found equally suitable for preparing DNA from coconut trunk tissues for<br />PCR analysis. The phytoplasmas aetiology of KW has been proved by the<br />nested PCR approach using P1/P7 and R16F2n/R16R2 primer<br />combinations. The study has further demonstrated that the nested PCR<br />approach can be employed to effectively detect the presence of<br />phytoplasma both in infected and in symptomless coconut trunk tissues.<br />Phytoplasma DNA was amplified from 95 out of 116 samples (81.9%).<br />Based on source of samples, phytoplasma DNA was amplified from KW<br />infected and symptomless samples, 95.1% and 67.3% respectively. This<br />study confirmed that KW is caused by phytoplasma.<br />Key words : Coconut, Cocos nucifera L., plant disease, Kalimantan wilt<br />disease, phytoplasma, polymerase chain reaction, Central<br />Kalimantan<br />ABSTRAK<br />Deteksi phytoplasma yang berasosiasi dengan penyakit<br />layu Kalimantan pada kelapa dengan reaksi rantai<br />polymerase<br />Kelapa merupakan komoditi sosial kedua setelah padi di Indonesia<br />dengan luasan areal lebih dari 3.6 juta ha pertanaman, ekuivalen dengan<br />sepertiga luas kelapa dunia, hal ini menjadikan Indonesia sebagai negara<br />produsen kelapa terluas di dunia. Sekarang ini produksi dan produktivitas<br />kelapa sangat rendah dan tidak stabil yang disebabkan oleh berbagai alasan<br />termasuk serangan hama dan penyakit. Penyakit layu Kalimantan telah<br />mengakibatkan kerugian yang besar pada pertanaman kelapa. Penelitian<br />sebelumnya untuk mengetahui penyebab penyakit dilakukan dengan<br />menguji bakteri, cendawan, virus, viroid dan patogen tanah seperti<br />nematoda tetapi tidak ada yang secara konsisten berasosiasi dengan<br />penyakit layu Kalimantan. Penelitian ini bertujuan untuk mendeteksi dan<br />mendiagnosa phytoplasma sebagai penyebab penyakit yang berasosiasi<br />dengan layu Kalimantan. Penelitian ini menggunakan dua metode untuk<br />mengekstraksi DNA yaitu metode CTAB yang biasanya menggunakan<br />nitrogen cair dimodifikasi dengan menghancurkan sampel tanaman pada<br />CTAB yang dipanaskan, dan metode skala kecil. Hasil penelitian<br />menunjukkan bahwa kedua metode yang digunakan menghasilkan DNA<br />yang sama baiknya untuk analisis PCR. Teknik nested PCR menggunakan<br />kombinasi primer P1/P7 dan R16F2n/R16R2 dapat membuktikan bahwa<br />penyebab penyakit layu Kalimantan adalah phytoplasma. Teknik ini juga<br />secara efektif dapat mendeteksi phytoplasma dalam jaringan tanaman<br />kelapa yang sudah terinfeksi maupun yang belum menunjukkan gejala<br />penyakit. DNA phytoplasma dapat dideteksi pada 95 sampel dari 116<br />sampel (81.9%) yang dianalisis. Berdasarkan jenis sample yang diperiksa<br />ternyata phytoplasma dapat dideteksi pada sample yang terinfeksi maupun<br />yang belum menunjukkan gejala penyakit masing-masing 95.1% dan<br />67.3%. Hasil penelitian ini mengkonfirmasi bahwa penyakit layu<br />Kalimantan disebabkan oleh phytoplasma.<br />Kata kunci: Kelapa, Cocos nucifera L., penyakit tanaman, penyakit layu<br />Kalimantan,  phytoplasma,  reaksi  rantai  polymerase,<br />Kalimantan Tengah

scholarly journals Evaluation of Five Methods for Total DNA Extraction from Western Corn Rootworm Beetles

PLoS ONE ◽  
2010 ◽  
Vol 5 (8) ◽  
pp. e11963 ◽  
Author(s):  
Hong Chen ◽  
Murugesan Rangasamy ◽  
Sek Yee Tan ◽  
Haichuan Wang ◽  
Blair D. Siegfried
Keyword(s):  
Dna Extraction ◽  
Western Corn Rootworm ◽  
Corn Rootworm ◽  
Total Dna
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