Component-Based Allergen-Microarray: Der p 2 and Der f 2 Dust Mite Sensitization Is More Common in Patients With Severe Asthma

2016 ◽  
Vol 26 (2) ◽  
pp. 141-143 ◽  
Author(s):  
L Sylvestre ◽  
J Jégu ◽  
C Metz-Favre ◽  
C Barnig ◽  
S Qi ◽  
...  
Keyword(s):  
Severe Asthma ◽  
Dust Mite ◽  
Der P 2 ◽  
Allergen Microarray

scholarly journals Antibody blockade of Dectin-2 suppresses house dust mite-induced Th2 cytokine production in dendritic cell- and monocyte-depleted peripheral blood mononuclear cell co-cultures from asthma patients

2019 ◽  
Vol 26 (1) ◽  
Author(s):  
Ming-Han Chen ◽  
Ming-Ting Huang ◽  
Wen-Kuang Yu ◽  
Shinn-Shing Lee ◽  
Jia-Horng Wang ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Specific Binding ◽  
Dependent Manner ◽  
Peripheral Blood Mononuclear ◽  
Dust Mite ◽  
Der P 2

Abstract Background Dectin-2, which is a C-type lectin, interacts with the house dust mite (HDM) Dermatophagoides pteronyssinus allergen. This study aimed to investigate whether Dectin-2 blockade by antagonistic monoclonal antibodies (MoAbs) attenuates HDM-induced allergic responses. Methods Two anti-Dectin-2 MoAbs were generated and validated for specific binding to Dectin-2 Fc fusion protein (Dectin-2.Fc) and inhibition of Dectin-2.Fc/HDM interaction. Patients with asthma exhibiting high titers of anti-D. pteronyssinus IgE were enrolled. Peripheral blood mononuclear cells with depleted CD14+ monocytes were obtained from these patients and co-cultured with autologous monocyte-derived conventional dendritic cells in the presence of D. pteronyssinus or its group 2 allergens (Der p 2). Interleukin (IL)-5 and IL-13 levels in the culture supernatants were determined using ELISA in the presence or absence of anti-Dectin-2 MoAbs. Results Two MoAbs, 6A4G7 and 17A1D10, showed specific binding to recombinant Dectin-2.Fc and inhibited HDM binding to Dectin-2.Fc. Both anti-Dectin-2 MoAbs inhibited IL-5 and IL-13 production in co-cultures with Der p 2 stimulation in a dose-dependent manner. 6A4G7 and 17A1D10 (3 μg/mL) significantly inhibited Der p 2-induced (3 μg/mL) IL-5 production by 69.7 and 86.4% and IL-13 production by 84.0 and 81.4%, respectively. Moreover, this inhibitory effect of the two MoAbs remained significant in the presence of D. pteronyssinus. Conclusions Anti-Dectin-2 MoAbs significantly inhibited HDM-induced allergic responses in vitro and therefore have the potential to become therapeutic agents in mite-induced allergic diseases.

scholarly journals Evaluation of the Role of IgE Responses to Der p 1 and Der p 2 in Chinese House Dust Mite-Allergic Patients

2015 ◽  
Vol 167 (3) ◽  
pp. 203-210 ◽  
Author(s):  
Hui-Ying Wang ◽  
Zhong-Shan Gao ◽  
Xiang Zhou ◽  
Yu Dai ◽  
Wo Yao ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Dust Mite ◽  
Der P 1 ◽  
Der P 2

Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates

2012 ◽  
Vol 58 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Kyoung Yong Jeong ◽  
In-Yong Lee ◽  
Tai-Soon Yong ◽  
Jae-Hyun Lee ◽  
Eun-Jin Kim ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Dust Mite ◽  
Sequence Polymorphisms ◽  
Der P 1 ◽  
Der P 2

A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation

FEBS Journal ◽  
2005 ◽  
Vol 272 (13) ◽  
pp. 3449-3460 ◽  
Author(s):  
Linda Johansson ◽  
Linda Svensson ◽  
Ulrika Bergström ◽  
Gunilla Jacobsson-Ekman ◽  
Elias S. J. Arnér ◽  
...  
Keyword(s):  
Mouse Model ◽  
Mite Allergen ◽  
Dust Mite ◽  
Dust Mite Allergen ◽  
Der P 2

scholarly journals Effect of Amino Acid Polymorphisms of House Dust Mite Der p 2 Variants on Allergic Sensitization

2016 ◽  
Vol 8 (1) ◽  
pp. 55 ◽  
Author(s):  
Sasipa Tanyaratsrisakul ◽  
Orathai Jirapongsananuruk ◽  
Bhakkawarat Kulwanich ◽  
Belinda J. Hales ◽  
Wayne R. Thomas ◽  
...  
Keyword(s):  
Amino Acid ◽  
House Dust ◽  
House Dust Mite ◽  
Allergic Sensitization ◽  
Dust Mite ◽  
Der P 2

Mimotopes identify conformational B-cell epitopes on the two major house dust mite allergens Der p 1 and Der p 2

2008 ◽  
Vol 45 (5) ◽  
pp. 1308-1317 ◽  
Author(s):  
Krisztina Szalai ◽  
Jan Fuhrmann ◽  
Tea Pavkov ◽  
Michael Scheidl ◽  
Julia Wallmann ◽  
...  
Keyword(s):  
B Cell ◽  
House Dust ◽  
House Dust Mite ◽  
B Cell Epitopes ◽  
Dust Mite ◽  
Der P 1 ◽  
Der P 2

Virus‐like particles displaying major house dust mite allergen Der p 2 for prophylactic allergen immunotherapy

Allergy ◽  
2020 ◽  
Vol 75 (5) ◽  
pp. 1232-1236 ◽  
Author(s):  
Tewarit Soongrung ◽  
Karntichar Mongkorntanyatip ◽  
Termsri Peepim ◽  
Sirikarn Jitthamstaporn ◽  
Patrawadee Pitakpolrat ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Allergen Immunotherapy ◽  
Mite Allergen ◽  
House Dust Mite Allergen ◽  
Virus Like Particles ◽  
Dust Mite ◽  
Dust Mite Allergen ◽  
Der P 2
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