scholarly journals Applications of Site-directed Spin Labeling Electron Paramagnetic Resonance to Studying the Structure, Dynamics and Interactions of Membrane Proteins

2012 ◽  
Author(s):  
Daniel Marlay Freed
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Membrane Proteins ◽  
Spin Labeling ◽  
Paramagnetic Resonance ◽  
Structure Dynamics ◽  
Site Directed Spin Labeling ◽  
Electron Paramagnetic

scholarly journals Electron Paramagnetic Resonance as a Tool for Studying Membrane Proteins

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 763 ◽  
Author(s):  
Indra D. Sahu ◽  
Gary A. Lorigan
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Membrane Proteins ◽  
Dynamic Properties ◽  
Conformational Dynamics ◽  
Spin Labeling ◽  
Paramagnetic Resonance ◽  
Hydrophobic Nature ◽  
Biophysical Techniques ◽  
Site Directed Spin Labeling ◽  
Electron Paramagnetic

Membrane proteins possess a variety of functions essential to the survival of organisms. However, due to their inherent hydrophobic nature, it is extremely difficult to probe the structure and dynamic properties of membrane proteins using traditional biophysical techniques, particularly in their native environments. Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling (SDSL) is a very powerful and rapidly growing biophysical technique to study pertinent structural and dynamic properties of membrane proteins with no size restrictions. In this review, we will briefly discuss the most commonly used EPR techniques and their recent applications for answering structure and conformational dynamics related questions of important membrane protein systems.

scholarly journals Site-Directed Spin Labeling EPR for Studying Membrane Proteins

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Indra D. Sahu ◽  
Gary A. Lorigan
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Membrane Proteins ◽  
Epr Spectroscopy ◽  
Dynamic Properties ◽  
Biological Systems ◽  
Spin Labeling ◽  
Paramagnetic Resonance ◽  
Site Directed Spin Labeling ◽  
Living Organisms ◽  
Electron Paramagnetic

Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy is a rapidly expanding powerful biophysical technique to study the structural and dynamic properties of membrane proteins in a native environment. Membrane proteins are responsible for performing important functions in a wide variety of complicated biological systems that are responsible for the survival of living organisms. In this review, a brief introduction of the most popular SDSL EPR techniques and illustrations of recent applications for studying pertinent structural and dynamic properties on membrane proteins will be discussed.

Nanoscale lipid membrane mimetics in spin-labeling and electron paramagnetic resonance spectroscopy studies of protein structure and function

2017 ◽  
Vol 6 (1) ◽  
pp. 75-92 ◽  
Author(s):  
Elka R. Georgieva
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Protein Structure ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Spin Labeling ◽  
Structure And Function ◽  
Protein Structure And Function ◽  
Paramagnetic Resonance ◽  
And Function ◽  
Electron Paramagnetic

AbstractCellular membranes and associated proteins play critical physiological roles in organisms from all life kingdoms. In many cases, malfunction of biological membranes triggered by changes in the lipid bilayer properties or membrane protein functional abnormalities lead to severe diseases. To understand in detail the processes that govern the life of cells and to control diseases, one of the major tasks in biological sciences is to learn how the membrane proteins function. To do so, a variety of biochemical and biophysical approaches have been used in molecular studies of membrane protein structure and function on the nanoscale. This review focuses on electron paramagnetic resonance with site-directed nitroxide spin-labeling (SDSL EPR), which is a rapidly expanding and powerful technique reporting on the local protein/spin-label dynamics and on large functionally important structural rearrangements. On the other hand, adequate to nanoscale study membrane mimetics have been developed and used in conjunction with SDSL EPR. Primarily, these mimetics include various liposomes, bicelles, and nanodiscs. This review provides a basic description of the EPR methods, continuous-wave and pulse, applied to spin-labeled proteins, and highlights several representative applications of EPR to liposome-, bicelle-, or nanodisc-reconstituted membrane proteins.

scholarly journals Posttranscriptional site-directed spin labeling of large RNAs with an unnatural base pair system under non-denaturing conditions

2020 ◽  
Vol 11 (35) ◽  
pp. 9655-9664
Author(s):  
Yan Wang ◽  
Venkatesan Kathiresan ◽  
Yaoyi Chen ◽  
Yanping Hu ◽  
Wei Jiang ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Epr Spectroscopy ◽  
Base Pair ◽  
Spin Labeling ◽  
Paramagnetic Resonance ◽  
Site Directed Spin Labeling ◽  
Electron Paramagnetic

Site-directed spin labeling (SDSL) of large RNAs for electron paramagnetic resonance (EPR) spectroscopy has remained challenging to date.

scholarly journals Characterization of the Linker 2 Region in Human Vimentin Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance†

Biochemistry ◽  
2006 ◽  
Vol 45 (39) ◽  
pp. 11737-11743 ◽  
Author(s):  
John F. Hess ◽  
Madhu S. Budamagunta ◽  
Rebecca L. Shipman ◽  
Paul G. FitzGerald ◽  
John C. Voss
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Spin Labeling ◽  
Paramagnetic Resonance ◽  
Site Directed Spin Labeling ◽  
Electron Paramagnetic
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