Single Vesicle Fusion Assay in Model Membranes to Study SNARE-medicated Membrane Fusion

2010 ◽  
Author(s):  
Marta Katarzyna Domanska
Keyword(s):  
Membrane Fusion ◽  
Vesicle Fusion ◽  
Model Membranes ◽  
Single Vesicle

scholarly journals Single-Vesicle Fusion Assay Reveals Munc18-1 Binding to the SNARE Core Is Sufficient for Stimulating Membrane Fusion

2010 ◽  
Vol 1 (3) ◽  
pp. 168-174 ◽  
Author(s):  
Jiajie Diao ◽  
Zengliu Su ◽  
Xiaobing Lu ◽  
Tae-Young Yoon ◽  
Yeon-Kyun Shin ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Vesicle Fusion ◽  
Single Vesicle

scholarly journals Molecular mechanisms of atlastin-mediated ER membrane fusion revealed by a FRET-based single-vesicle fusion assay

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Kyung Tae Kim ◽  
Yeojin Moon ◽  
Yunsu Jang ◽  
Kang Taek Lee ◽  
Changwook Lee ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Molecular Mechanisms ◽  
Vesicle Fusion ◽  
Single Vesicle ◽  
Er Membrane

scholarly journals Studying calcium-triggered vesicle fusion in a single vesicle-vesicle content and lipid-mixing system

2012 ◽  
Vol 8 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Minjoung Kyoung ◽  
Yunxiang Zhang ◽  
Jiajie Diao ◽  
Steven Chu ◽  
Axel T Brunger
Keyword(s):  
Vesicle Fusion ◽  
Vesicle Content ◽  
Single Vesicle

scholarly journals Induced Membrane Permeabilization and Vesicle Fusion: Synthetic Antimicrobials Acting on Model Membranes

2020 ◽  
Vol 118 (3) ◽  
pp. 383a
Author(s):  
Shuai Shi ◽  
Ndjali Quarta ◽  
Runhui Liu ◽  
Maria Hoernke
Keyword(s):  
Vesicle Fusion ◽  
Membrane Permeabilization ◽  
Model Membranes ◽  
Induced Membrane

Elucidation of the mechanism and energy barrier for anesthetic triggered membrane fusion in model membranes

2019 ◽  
Vol 97 (6) ◽  
pp. 474-482
Author(s):  
Trinh T. Nguyen ◽  
David T. Cramb
Keyword(s):  
Transition State ◽  
Membrane Fusion ◽  
Energy Barrier ◽  
Activation Barrier ◽  
Correlation Spectroscopy ◽  
Model Membranes ◽  
General Anesthetics ◽  
New Class ◽  
Two Photon ◽  
Photon Excitation

Membrane fusion is vital for cellular function and is generally mediated via fusogenic proteins and peptides. The mechanistic details and subsequently the transition state dynamics of membrane fusion will be dependent on the type of the fusogenic agent. We have previously established the potential of general anesthetics as a new class of fusion triggering agents in model membranes. We employed two-photon excitation fluorescence cross-correlation spectroscopy (TPE-FCCS) to report on vesicle association kinetics and steady-state fluorescence dequenching assays to monitor lipid mixing kinetics. Using halothane to trigger fusion in 110 nm diameter dioleoylphosphatidylcholine (DOPC) liposomes, we found that lipid rearrangement towards the formation of the fusion stalk was rate limiting. The activation barrier for halothane induced membrane fusion in 110 nm vesicles was found to be ∼40 kJ mol−1. We calculated the enthalpy and entropy of the transition state to be ∼40 kJ mol−1 and ∼180 J mol−1 K−1, respectively. We have found that the addition of halothane effectively lowers the energy barrier for membrane fusion in less curved vesicles largely due to entropic advantages.

scholarly journals A fast, single-vesicle fusion assay mimics physiological SNARE requirements

2010 ◽  
Vol 107 (8) ◽  
pp. 3517-3521 ◽  
Author(s):  
Erdem Karatekin ◽  
Jérôme Di Giovanni ◽  
Cécile Iborra ◽  
Jeff Coleman ◽  
Ben O'Shaughnessy ◽  
...  
Keyword(s):  
Vesicle Fusion ◽  
Single Vesicle

scholarly journals SNARE-Mediated Single-Vesicle Fusion Events with Supported and Freestanding Lipid Membranes

2017 ◽  
Vol 113 (11) ◽  
pp. 2573-2574 ◽  
Author(s):  
Jan W. Kuhlmann ◽  
Meike Junius ◽  
Ulf Diederichsen ◽  
Claudia Steinem
Keyword(s):  
Lipid Membranes ◽  
Vesicle Fusion ◽  
Single Vesicle
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