scholarly journals Single-Vesicle Fusion Assay Reveals Munc18-1 Binding to the SNARE Core Is Sufficient for Stimulating Membrane Fusion

2010 ◽  
Vol 1 (3) ◽  
pp. 168-174 ◽  
Author(s):  
Jiajie Diao ◽  
Zengliu Su ◽  
Xiaobing Lu ◽  
Tae-Young Yoon ◽  
Yeon-Kyun Shin ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Vesicle Fusion ◽  
Single Vesicle

scholarly journals Molecular mechanisms of atlastin-mediated ER membrane fusion revealed by a FRET-based single-vesicle fusion assay

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Kyung Tae Kim ◽  
Yeojin Moon ◽  
Yunsu Jang ◽  
Kang Taek Lee ◽  
Changwook Lee ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Molecular Mechanisms ◽  
Vesicle Fusion ◽  
Single Vesicle ◽  
Er Membrane

scholarly journals Studying calcium-triggered vesicle fusion in a single vesicle-vesicle content and lipid-mixing system

2012 ◽  
Vol 8 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Minjoung Kyoung ◽  
Yunxiang Zhang ◽  
Jiajie Diao ◽  
Steven Chu ◽  
Axel T Brunger
Keyword(s):  
Vesicle Fusion ◽  
Vesicle Content ◽  
Single Vesicle

scholarly journals A fast, single-vesicle fusion assay mimics physiological SNARE requirements

2010 ◽  
Vol 107 (8) ◽  
pp. 3517-3521 ◽  
Author(s):  
Erdem Karatekin ◽  
Jérôme Di Giovanni ◽  
Cécile Iborra ◽  
Jeff Coleman ◽  
Ben O'Shaughnessy ◽  
...  
Keyword(s):  
Vesicle Fusion ◽  
Single Vesicle

scholarly journals SNARE-Mediated Single-Vesicle Fusion Events with Supported and Freestanding Lipid Membranes

2017 ◽  
Vol 113 (11) ◽  
pp. 2573-2574 ◽  
Author(s):  
Jan W. Kuhlmann ◽  
Meike Junius ◽  
Ulf Diederichsen ◽  
Claudia Steinem
Keyword(s):  
Lipid Membranes ◽  
Vesicle Fusion ◽  
Single Vesicle

scholarly journals High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated Analysis

Langmuir ◽  
2018 ◽  
Vol 34 (20) ◽  
pp. 5849-5859 ◽  
Author(s):  
Sathish Ramakrishnan ◽  
Andrea Gohlke ◽  
Feng Li ◽  
Jeff Coleman ◽  
Weiming Xu ◽  
...  
Keyword(s):  
High Throughput ◽  
Automated Analysis ◽  
Vesicle Fusion ◽  
Single Vesicle

scholarly journals A single vesicle-vesicle fusion assay for in vitro studies of SNAREs and accessory proteins

2012 ◽  
Vol 7 (5) ◽  
pp. 921-934 ◽  
Author(s):  
Jiajie Diao ◽  
Yuji Ishitsuka ◽  
Hanki Lee ◽  
Chirlmin Joo ◽  
Zengliu Su ◽  
...  
Keyword(s):  
Vesicle Fusion ◽  
In Vitro Studies ◽  
Accessory Proteins ◽  
Single Vesicle

scholarly journals v-SNARE transmembrane domains function as catalysts for vesicle fusion

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Madhurima Dhara ◽  
Antonio Yarzagaray ◽  
Mazen Makke ◽  
Barbara Schindeldecker ◽  
Yvonne Schwarz ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Conformational Flexibility ◽  
Vesicle Fusion ◽  
Transmembrane Domains ◽  
Fusion Process ◽  
Snare Proteins ◽  
Fusion Pore ◽  
Structural Flexibility ◽  
Outer Leaflet ◽  
Pore Expansion

Vesicle fusion is mediated by an assembly of SNARE proteins between opposing membranes, but it is unknown whether transmembrane domains (TMDs) of SNARE proteins serve mechanistic functions that go beyond passive anchoring of the force-generating SNAREpin to the fusing membranes. Here, we show that conformational flexibility of synaptobrevin-2 TMD is essential for efficient Ca2+-triggered exocytosis and actively promotes membrane fusion as well as fusion pore expansion. Specifically, the introduction of helix-stabilizing leucine residues within the TMD region spanning the vesicle’s outer leaflet strongly impairs exocytosis and decelerates fusion pore dilation. In contrast, increasing the number of helix-destabilizing, ß-branched valine or isoleucine residues within the TMD restores normal secretion but accelerates fusion pore expansion beyond the rate found for the wildtype protein. These observations provide evidence that the synaptobrevin-2 TMD catalyzes the fusion process by its structural flexibility, actively setting the pace of fusion pore expansion.

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