scholarly journals Possibility of Using Flow Cytometry in the Treated Ballast Water Quality Detection

2016 ◽  
Vol 51 (1) ◽  
pp. 43-55 ◽  
Author(s):  
Goran Bakalar ◽  
Vinko Tomas

In this article, monitoring of ballast water after its treatment by any of BWTS (Ballast Water Treatment System) on board ships has been analyzed. The efficiency of those systems has shown to be the major problem as there are no systems for tracking ship ballast operations. The overall aim of the study was to emphasize the necessity of monitoring the ballast water treatment on board ships and to propose a solution. The flow cytometry technology and applications of flow cytometers have been analyzed as well. The functionality and possibility of using this technology for detection of the treated ballast water quality has been explained. The results of the flow cytometry detection have been confirmed mathematically. The possibility of finding the remaining microorganisms in the treated ballast water has been calculated and the result was a very high percentage of 82%. The study presented in this paper aids in the understanding of how important it is for results of the ballast water treatment systems operation to be monitored since such monitoring is also in the interest of protecting the environment.

2017 ◽  
Author(s):  
Seungshic Yum ◽  
Bong Gil Hyun ◽  
Kitae Rhie ◽  
Kyoungsoon Shin

Rapid and simple analytical methods for viable microorganism detection in ballast water are required to evaluate the efficiency of ballast water treatment system. During the course of systematic investigation of the cytotoxicity and apoptosis assays, it was found that the adenosine triphosphate (ATP) and luminescence based cell viability assay, in other word, an ATP assay was the most sensitive and applicable to ballast water management (BWM). The assay was applied to cultured microalgae samples, and it could detect the existence of 5 viable cells in 100 μl. Comparably low luminescent values were detected in two cultured diatom species than in cultured dinoflagellates. This result might be caused by the small cell volume in diatom species. Following a regression model between ATP concentration and cell volume, an ATP guideline (876 – 109246 relative luminescence units: RLU) was developed for the evaluation of treated ballast water. ATP assay was also applied to the evaluation of ballast water treatment system (BWTS). The luminescence value which obtained from the ATP assay also showed a good correlation with the presence of living natural plankton cells with comparably low luminescence values than the cultured species. The low ATP concentration in natural plankton cells may reflect a decline in their biological activity because of extended exposure to dark conditions. ATP assay could be a suitable method for the monitoring of ballast water management compliance even though the results of this study need further validation.


2007 ◽  
Vol 10 (4) ◽  
pp. 397-407 ◽  
Author(s):  
Rustom Mamlook ◽  
Omar Badran ◽  
Mazen M. Abu-Khader ◽  
Arne Holdo ◽  
Jason Dales

2010 ◽  
Vol 9 (2) ◽  
pp. 213-222 ◽  
Author(s):  
Marcel Veldhuis ◽  
Cato ten Hallers ◽  
Etienne Brutel de la Rivière ◽  
Frank Fuhr ◽  
Jan Finke ◽  
...  

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