scholarly journals Cold-Shock Test Is a Practical Method for Selecting Boar Ejaculates Yielding Appropriate Seminal Plasma for Post-Thawing Supplementation

2021 ◽  
Author(s):  
Felipe Martínez Pastor
Keyword(s):  
Seminal Plasma ◽  
Cold Shock ◽  
Practical Method ◽  
Shock Test

scholarly journals Cold-Shock Test Is a Practical Method for Selecting Boar Ejaculates Yielding Appropriate Seminal Plasma for Post-Thawing Supplementation

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 871
Author(s):  
Estíbaliz Lacalle ◽  
Andrea Núñez ◽  
Estela Fernández-Alegre ◽  
Itxaso Crespo-Félez ◽  
Juan Carlos Domínguez ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Cold Shock ◽  
Practical Method ◽  
Sperm Viability ◽  
Disulfide Bridges ◽  
Kinematic Parameters ◽  
Shock Test ◽  
Progressive Motility ◽  
Neat Semen

Artificial insemination (AI) with cryopreserved semen is still unreliable for extensive pig industry application. Adding seminal plasma (SP) could improve post-thawing quality, but its suitability could vary. We applied a simple cold-shock test (CST, 5 min at 0 °C) on neat semen for classifying ejaculates (n = 63) as resistant or sensitive, obtaining two SP pools (CST-resistant: SPr, sensitive: SPs). Subsequently, frozen/thawed spermatozoa from six boars were incubated (37 °C) in MR-A® extender (control), 20% SPr, or 20% SPs, and analyzed at 0, 2, and 4 h. SP improved total and progressive motility, with a higher effect for SPr and STR (p < 0.05), decreasing kinematic parameters VCL and VAP, ALH, and BCF. Sperm viability was unaffected. SP increased apoptotic and membrane disorder ratios, and acrosomal damage, not affecting the chromatin structure (DNA fragmentation and immaturity by SCSA), protamination (CMA3), or disulfide levels (mBBr). However, the proportion of spermatozoa with elevated free thiols (disulfide bridges reduction) significantly increased. Results support a stimulatory role of SP on thawed semen, with additional benefits from SPr. The effect of SP and especially SPr after AI should be tested since CST could be a practical test for selecting suitable ejaculates in AI centers.

scholarly journals Seminal Plasma Proteins Revert the Cold-Shock Damage on Ram Sperm Membrane1

2000 ◽  
Vol 63 (5) ◽  
pp. 1531-1537 ◽  
Author(s):  
Beatriz Barrios ◽  
Rosaura Pérez-Pé ◽  
Margarita Gallego ◽  
Agustín Tato ◽  
Jesús Osada ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Cold Shock ◽  
Seminal Plasma Proteins ◽  
Ram Sperm

THE SUSCEPTIBILITY OF SPERMATOZOA TO TEMPERATURE SHOCK

1959 ◽  
Vol 19 (3) ◽  
pp. 211-220 ◽  
Author(s):  
R. G. WALES ◽  
I. G. WHITE
Keyword(s):  
High Temperatures ◽  
Seminal Plasma ◽  
Room Temperature ◽  
Cold Shock ◽  
Differential Staining ◽  
Temperature Shock

SUMMARY The susceptibility of bull, ram, rabbit, dog, human and fowl spermatozoa to cold shock and high temperatures has been assessed. Motility and differential staining were used as criteria. Ram and bull spermatozoa were increasingly affected by cold shock at temperatures below 15° C; other spermatozoa were, however, little affected. Epididymal ram spermatozoa, particularly those with an attached kinoplasmic droplet, were more resistant than ejaculated ones; the addition of seminal plasma had little effect. Second ejaculates from bulls were slightly more resistant than first ejaculates. Washing bull or fowl spermatozoa free of seminal plasma did not influence their susceptibility to cold shock. Five min at 50° C severely depressed the motility of all spermatozoa except those of the fowl which were, however, completely immobilized at 55° C. Most spermatozoa took up stain more readily when mixed with it at high temperatures than when brought back to room temperature and then mixed; this is due to an increase in the toxicity of the stain at high temperatures.

Ultrastructural study of the ability of seminal plasma proteins to protect ram spermatozoa against cold-shock

2009 ◽  
Vol 72 (8) ◽  
pp. 566-572 ◽  
Author(s):  
Carmen Colás ◽  
Concepción Junquera ◽  
Rosaura Pérez-Pé ◽  
José A. Cebrián-Pérez ◽  
Teresa Muiño-Blanco
Keyword(s):  
Seminal Plasma ◽  
Ultrastructural Study ◽  
Plasma Proteins ◽  
Cold Shock ◽  
Seminal Plasma Proteins

scholarly journals Effect of bull, diluter and LDL-cholesterol concentration on spermatozoa resistance against cold shock

2013 ◽  
Vol 61 (6) ◽  
pp. 1575-1581 ◽  
Author(s):  
Jan Beran ◽  
Ondřej Šimoník ◽  
Luděk Stádník ◽  
Radko Rajmon ◽  
Jaromír Ducháček ◽  
...  
Keyword(s):  
Cold Shock ◽  
Ldl Cholesterol ◽  
Egg Yolk ◽  
Cholesterol Concentration ◽  
Shock Test ◽  
Subsequent Research ◽  
Live Sperm ◽  
Required Quality ◽  
Fresh Semen

The objectives of this study were to determine and evaluate the effect of bull, diluter and addition of LDL in different concentration on the percentage rate of spermatozoa survival after cold shock. In total, four bulls were collected during a period of eight weeks. A total of 8 samples of fresh semen with required quality were processed. Three extenders were used for dilution of each sample; AndroMed®, Bioxcell® and Triladyl®, each in standard and LDL enriched variants. In the case of AndroMed® and Bioxcell®, 4, 6 and 8% of LDL were simply added. In Triladyl®, 6, 8 and 10% of LDL replaced the standard egg yolk component. Resistance of spermatozoa against cold shock (0 °C, 10 minutes) was evaluated by the percentage rate of live sperm using Eosin-Nigrosine staining immediately and 2 hours after heat incubation (37 °C). The results showed the influence of bull individuality as an important factor. Among diluters used it is possible to recommend AndroMed® and Bioxcell® due to significantly (P < 0.01) lower decline of live sperm proportion during the cold shock test than Triladyl® (-9.19, respectively -4.95%). The optimal LDL concentration increasing resistance of spermatozoa against cold shock was not determined, therefore subsequent research is necessary.

The effect of cold shock and deep-freezing on ram spermatozoa collected by electrical ejaculation and by an artificial vagina

1968 ◽  
Vol 19 (1) ◽  
pp. 119 ◽  
Author(s):  
PJ Quinn ◽  
S Salamon ◽  
IG White
Keyword(s):  
Seminal Plasma ◽  
Congo Red ◽  
Cold Shock ◽  
Rapid Freezing ◽  
Staining Reaction ◽  
Deep Freezing ◽  
Freezing Method ◽  
Low Levels ◽  
Artificial Vagina ◽  
Better Than

Spermatozoa collected with an artificial vagina (AV) were more resistant to cold shock as judged by motility and staining reaction than when ejaculated electrically (EE). Dilution of AV spermatozoa in AV seminal plasma caused some increase in susceptibility to cold shock, particularly as judged by motility scores, but the evidence suggests that EE seminal plasma renders spermatozoa more susceptible than does AV seminal plasma. Spermatozoa exposed to cold shock immediately after collection were most permeable to Congo red and became increasingly impermeable when incubated at 30°C; the increase in resistance of AV spermatozoa was greater than that of EE spermatozoa. When 10 successive ejaculates were collected from rams by the artificial vagina there was no great change in volume but the spermatozoal concentration declined. After cold shock, the percentage of unstained spermatozoa fell to very low levels by the fifth ejaculate and there was also a decline in motility. The viability of semen thawed after freezing to –79°C by rapid freezing method was better than when a slow freezing method was used, particularly when the semen was collected with an artificial vagina.

scholarly journals Phospholipid and Cholesterol Content of Epididymal and Jaculated Ram Spermatozoa and Seminal Plasma in Elation to Cold Shock

1967 ◽  
Vol 20 (6) ◽  
pp. 1205 ◽  
Author(s):  
PJ Quinn ◽  
IG White
Keyword(s):  
Seminal Plasma ◽  
Cold Shock ◽  
Total Phospholipid ◽  
Phospholipid Composition ◽  
Cholesterol Content

The concentration of total phospholipid, phosphatidylcholine, phosphatidylethanolamine, and choline plasmalogen in spermatozoa from the caput epididymis of the ram was higher than in spermatozoa from the cauda. The phospholipid composition of spermatozoa from the latter region of the epididymis more closely resembled ejaculated spermatozoa.

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