scholarly journals PRODUCTION OF EXTRACELLULAR PIGMENT FROM MICROBES AND ITS APPLICATION

2015 ◽  
Vol 9 (2) ◽  
pp. 23-29 ◽  
Author(s):  
V Navin raju ◽  
T Radha
Keyword(s):  
Extracellular Pigment

Effect of light on growth, intracellular and extracellular pigment production by five pigment-producing filamentous fungi in synthetic medium

2010 ◽  
Vol 109 (4) ◽  
pp. 346-350 ◽  
Author(s):  
Palanivel Velmurugan ◽  
Yong Hoon Lee ◽  
Chidambaram Kulandaisamy Venil ◽  
Perumalsamy Lakshmanaperumalsamy ◽  
Jong-Chan Chae ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Synthetic Medium ◽  
Pigment Production ◽  
Extracellular Pigment ◽  
Effect Of Light

Plasmid-determined 2-hydroxypyridine utilization by Arthrobacter crystallopoietes

1979 ◽  
Vol 25 (3) ◽  
pp. 329-334 ◽  
Author(s):  
M. Weinberger ◽  
P. E. Kolenbrander
Keyword(s):  
Molecular Weight ◽  
Plasmid Dna ◽  
Cryptic Plasmid ◽  
Carbon And Nitrogen ◽  
Arthrobacter Crystallopoietes ◽  
Extracellular Pigment

Arthrobacter crystallopoietes has the ability to utilize 2-hydroxypyridine (2-HP) as a source of carbon and nitrogen and forms a blue extracellular pigment when grown in the presence of 2-HP. Ultracentrifugal analyses of pigment producing(Pig+) and pigment nonproducing(Pig−) strains of A. crystallopoietes revealed the presence of plasmid material in both strains. Recovery of plasmid DNA from Pig+ strains is two or three times greater than from Pig− strains. The molecular weight of plasmid DNA recovered from Pig+ strains (63 Mdaltons) is slightly higher than the molecular weight of plasmid DNA from Pig− strains. Consistent with the characterization of plasmid DNA from the two strains is that Pig+ strains contain a 63-Mdalton plasmid encoding 2-HP utilization as well as a cryptic plasmid of very nearly equal molecular weight. Pig− strains contain only the cryptic plasmid.

STUDIES OF PIGMENTATION BY PSEUDOMONAS INDIGOFERA

1961 ◽  
Vol 7 (4) ◽  
pp. 577-585 ◽  
Author(s):  
E. C. Sommer ◽  
W. S. Silver ◽  
L. C. Vining
Keyword(s):  
Amino Acids ◽  
Spectral Data ◽  
Aspartic Acid ◽  
Synthetic Medium ◽  
Chemical Properties ◽  
Pond Water ◽  
Extracellular Pigment

A pseudomonad which deposited a large quantity of a blue extracellular pigment on the top of the colony was isolated from soil and pond water. It was found to be identical both physiologically and morphologically with Pseudomonas indigofera. Good pigmentation was obtained in a synthetic medium containing methionine, glutamine, arginine, and aspartic acid. The addition of other amino acids reduced growth and/or pigmentation. Chemical properties as well as spectral data strongly suggest that the pigment is indigoidine.

scholarly journals Ultrastructural Changes in Human Trabecular Meshwork Tissue after Laser Trabeculoplasty

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Jeffrey R. SooHoo ◽  
Leonard K. Seibold ◽  
David A. Ammar ◽  
Malik Y. Kahook
Keyword(s):  
Electron Microscopy ◽  
Trabecular Meshwork ◽  
Ex Vivo ◽  
Argon Laser ◽  
Ultrastructural Changes ◽  
Laboratory Evaluation ◽  
Tissue Destruction ◽  
Laser Trabeculoplasty ◽  
Pigment Granules ◽  
Extracellular Pigment

Purpose. To compare morphologic changes in human trabecular meshwork (TM) after selective laser trabeculoplasty (SLT) and argon laser trabeculoplasty (ALT).Design. Laboratory evaluation of ex vivo human eye TM after laser trabeculoplasty.Methods. Corneoscleral rims from human cadaver eyes were sectioned and treated with varying powers of either SLT or ALT. Specimens were examined using light microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM).Results. TEM of SLT at all powers resulted in disrupted TM cells with cracked and extracellular pigment granules. SEM of SLT samples treated at high power revealed tissue destruction with scrolling of trabecular beams. SEM of ALT-treated tissue showed increasing destruction with exposure to higher power. The presence or absence of “champagne” bubbles during SLT did not alter the histologic findings.Conclusions. SLT-treated human TM revealed disruption of TM cells with cracked, extracellular pigment granules, particularly at higher treatment powers. Tissue scrolling was noted at very high SLT energy levels. ALT-treated tissue showed significant damage to both the superficial and deeper TM tissues in a dose-dependent fashion. Further studies are needed to guide titration of treatment power to maximize the IOP-lowering effect while minimizing both energy delivered and damage to target tissues.

scholarly journals Biosynthesis of Silver Nanoparticles Mediated by Extracellular Pigment from Talaromyces purpurogenus and Their Biomedical Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 1042 ◽  
Author(s):  
Sharad Bhatnagar ◽  
Toshiro Kobori ◽  
Deepak Ganesh ◽  
Kazuyoshi Ogawa ◽  
Hideki Aoyagi
Keyword(s):  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Positive Control ◽  
Significant Activity ◽  
Hek 293 ◽  
Transmission Electron ◽  
Elemental Silver ◽  
Human Liver Cancer ◽  
Biosynthesized Agnps ◽  
Extracellular Pigment

In recent years, green syntheses have been researched comprehensively to develop inexpensive and eco-friendly approaches for the generation of nanoparticles. In this context, plant and microbial sources are being examined to discover potential reducing agents. This study aims to utilize an extracellular pigment produced by Talaromyces purpurogenus as a prospective reducing agent to synthesize silver nanoparticles (AgNPs). Biosynthesized AgNPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), electron probe micro analyser (EPMA), and zeta potential. The pigment functional groups involved in the generation of AgNPs were investigated using Fourier transform infrared spectroscopy. TEM images showed that the generated nanoparticles were spherical, hexagonal, rod-shaped, and triangular-shaped with a particle size distribution from 4 to 41 nm and exhibited a surface plasmon resonance at around 410 nm. DLS and zeta potential studies revealed that the particles were polydispersed and stable (−24.8 mV). EPMA confirmed the presence of elemental silver in the samples. Biosynthesized AgNPs exhibited minimum inhibitory concentrations of 32 and 4 μg/mL against E. coli and S. epidermidis, respectively. Further, cytotoxicity of the AgNPs was investigated against human cervical cancer (HeLa), human liver cancer (HepG2), and human embryonic kidney (HEK-293) cell lines using 5-fluorouracil as a positive control. A significant activity was recorded against HepG2 cell line with a half-maximal inhibitory concentration of 11.1 μg/mL.

scholarly journals Effect of amino acids on pigments, citrinin, and lovastatin production by Monascus purpureus under static conditions 

Biologija ◽  
2017 ◽  
Vol 63 (2) ◽  
Author(s):  
Padmavathi Talapragada ◽  
Rashmi Dikshit ◽  
Mudaheranwa Phocas ◽  
Madhusudan. M.R. ◽  
Sanjana Samprathi
Keyword(s):  
Amino Acids ◽  
Secondary Metabolites ◽  
Growth Medium ◽  
Uv Light ◽  
Submerged Cultivation ◽  
Red Pigment ◽  
Experimental Conditions ◽  
Potato Dextrose Broth ◽  
Extracellular Pigment ◽  
Static Conditions

Monascus spp. is known to produce many secondary metabolites including pigments, statin, and undesired mycotoxin. M. purpureus MTCC 410 strain was grown statically for ten days in potato dextrose broth supplemented with 1% UV sterilized amino acids. Whatmann filter No. 1 was used to separate the developed mycelia from the broth to get the intracellular pigment as the extracellular one is left in the filtrate. The pigment concentration was estimated by the calorimetric method for different wavelengths and expressed in colour value units (CVU). The presence of citrinin in the growth medium was checked under UV light at 350 nm and quantification was done with highperformance liquid chromatography column along with loop injector of 20 μl, and Shimadzu CLASS-VP version 5.032 software. The maximum biomass (143.6 g/l) was observed with supplementation of 1% D-serine to the medium, whereas the maximum intracellular pigment yield was observed with supplementation of L-histidine monohydrochoride (yellow – 4.48, orange – 3.97 and red pigment – 2.0 CVU/ml). The maximum extracellular pigment yield was observed with supplementation of glycine (yellow – 2.18, orange – 1.65 and red pigment – 1.38 CVU/ml to the growth medium). The maximum lovastatin yield was observed with supplementation of L-cysteine mono hydrochloride and concentration of 2064 mg/l. Maximum citrinin (1.29 mg/l) was observed with supplementation of DL-norleucine to the growht medium. M. purpureus requires suitable concentration of organic nitrogen in the form of amino acids for a higher yield of secondary metabolites such as supplementation of 1% L-cysteine monohydrochloride or L-tyrosine in the growth medium under submerged cultivation. None of the tested amino acids produced citrinin under experimental conditions making the outcome beneficial for industrial purposes.

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