Comparison of NCD and CNN based mitotic classification of neural stem cells in phase contrast microscopy

2021 ◽  
Author(s):  
Michael Marino
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Phase Contrast ◽  
Phase Contrast Microscopy ◽  
Contrast Microscopy

Automated Classification of Apoptosis in Phase Contrast Microscopy Using Capsule Network

2020 ◽  
Vol 39 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Aryan Mobiny ◽  
Hengyang Lu ◽  
Hien V. Nguyen ◽  
Badrinath Roysam ◽  
Navin Varadarajan
Keyword(s):  
Phase Contrast ◽  
Phase Contrast Microscopy ◽  
Automated Classification ◽  
Contrast Microscopy

scholarly journals Platelet-Derived Serotonin Stimulates TPO Production from Bone Marrow Mesenchymal Stromal Cells but Not Hepatocytes

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 4773-4773
Author(s):  
En Yu Liang ◽  
Jie Yu Ye ◽  
Li Xia Zhou ◽  
Chunfu Li ◽  
Beng H Chong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Flow Cytometry ◽  
Stromal Cells ◽  
Untreated Control ◽  
Phase Contrast ◽  
Fold Increase ◽  
Rna Expression ◽  
Phase Contrast Microscopy ◽  
Contrast Microscopy

Abstract In circulation thrombopoietin (TPO) level and platelet count is inversely related, but the precise controlling mechanism is still unknown. TPO is constitutively produced by the liver but can be induced to generate from bone marrow mesenchymal stromal cells (MSCs) in pathological conditions such as thrombocytopenia. Since serotonin (5-HT) can be released from the active platelets when the body undergoing severe thrombocytopenia or stress, it may function as an essential humoral mediator regulating TPO release. Our previous studies suggested that 5-HT enhances the growth of the colony-forming unit-fibroblast (CFU-F) from bone marrow, a progenitor of MSCs (Yang et al, Stem Cells, 2007; Ye et al, Stem Cells, 2014). However, the exact functions of 5-HT on MSCs and especially MSCs-derived TPO production are still under investigation. The bone marrow (BM) microenvironment may play an important role in the regulation of thrombopoiesis through release of thrombopoietic growth factor TPO. In this study, the expression of 5-HT2BR was identified in hMSCs and hepatocytic cell line MIHA by Q-PCR. 5-HT (200nM) promoted the proliferation of MSCs and MIHA cells by CCK-8 and MTT assay, respectively. This effect was abolished by 5-HT2BR blocker Ketanserin (2.0uM) in these cells. TPO RNA expression in hMSCs was enhanced in a time-dependent manner in response to the 5-HT treatment but not in MIHA cells. The maximum reaction peak was observed at 24 hrs with 1.5-fold increase compared to the untreated control in hMSCs. A significant increase TPO protein was found in the supernatants secreted by 5-HT-treated hMSCs. The changes measured by ELISA and cytokinearray and were 4.5-fold and 2.14-fold increased compared to the control sample. In addition to the evaluation of the effect of 5-HT on naked-TPO release from MSCs, we also investigate its impacts on the RNA expression of TPO contained within the MSC-derived microparticles (MPs). 5-HT was added to the hMSC culture for 24hrs, 36hrs and 48hrs respectively. It significantly stimulated MPs released from MSCs detected by flow cytometry. The maximal response time was observed at 36hrs. The AnnexinV positive population increased remarkably under 5-HT treatment. HtMSC Cell movement and MPs released were visualized under phase contrast microscopy. HtMSC cells with 5-HT treatment were stained with AnnexinV-PE and the plates were inspected under the Tirf microscopy to trace the shedding process of MPs. By using phase contrast microscopy, a notable generation of MPs was observed from approximate 6hrs after the addition of 5HT, while only a little amount of MPs were detected in the untreated control group. Budding MPs were observed in 5HT-treated MSCs under both SEM and TEM. The morphology of these MPs was compatible to those published in other studies. And these results were complementary to our previous data obtained by flow cytometry and Tirf microscopy. Our data proved that hMSCs-derived MPs contained inducible TPO mRNA, which was enhanced under 5-HT treatment with more than 10-fold increase compared to the untreated control. We further examined protein level of TPO in MPs using ELISA. The TPO protein level was increased more than 3-fold in 5-HT treated sample. Our findings suggested that 5-HT stimulated TPO released from MSCs in both dissociative and MP-bounded form, which indirectly promotes megakaryopoiesis and thrombopoiesis. Disclosures Yang: National Natural Science Foundation of China(81270580): Research Funding.

scholarly journals Rough Dental Implant Surfaces and Peri-Implantitis: Role of Phase-Contrast Microscopy, Laser Protocols, and Modified Home Oral Hygiene in Maintenance. A 10-Year Retrospective Study

2021 ◽  
Vol 11 (11) ◽  
pp. 4985
Author(s):  
Gianluigi Caccianiga ◽  
Gérard Rey ◽  
Paolo Caccianiga ◽  
Alessandro Leonida ◽  
Marco Baldoni ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Phase Contrast ◽  
Vertical Movement ◽  
Subgingival Plaque ◽  
Phase Contrast Microscopy ◽  
Implant Surface ◽  
Total Percentage ◽  
Contrast Microscopy

The aim of this study was to evaluate two different kinds of rough implant surface and to assess their tendency to peri-implantitis disease, with a follow-up of more than 10 years. Data were obtained from a cluster of 500 implants with Ti-Unite surface and 1000 implants with Ossean surface, with a minimum follow-up of 10 years. Implants had been inserted both in pristine bone and regenerated bone. We registered incidence of peri-implantitis and other causes of implant loss. All patients agreed with the following maintenance protocol: sonic brush with vertical movement (Broxo), interdental brushes, and oral irrigators (Broxo) at least two times every day. For all patients with implants, we evaluated subgingival plaque samples by phase-contrast microscopy every 4 months for a period of more than 10-years. Ti-Unite surface implants underwent peri-implantitis in 1.6% of the total number of implants inserted and Ossean surface implants showed peri-implantitis in 1.5% of the total number of implants. The total percentage of implant lost was 4% for Ti-Unite surfaces and 3.6% for Ossean surfaces. Strict control of implants leads to low percentage of peri-implantitis even for rough surfaces dental implants.

scholarly journals Coherent noise reduction in digital holographic phase contrast microscopy by slightly shifting object

2011 ◽  
Vol 19 (5) ◽  
pp. 3862 ◽  
Author(s):  
Feng Pan ◽  
Wen Xiao ◽  
Shuo Liu ◽  
FanJing Wang ◽  
Lu Rong ◽  
...  
Keyword(s):  
Noise Reduction ◽  
Phase Contrast ◽  
Phase Contrast Microscopy ◽  
Coherent Noise ◽  
Contrast Microscopy
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