scholarly journals Effects of Alkaline Pretreatment on the Characteristics of Collagen from Mangrove Conch (Telescopium telescopium)

2019 ◽  
Vol 22 (2) ◽  
pp. 355-365
Author(s):  
Sri Purwaningsih ◽  
Riyan Triono
Keyword(s):  
Acetic Acid ◽  
Protein Content ◽  
Extraction Yield ◽  
Proline Content ◽  
Alkaline Pretreatment ◽  
Mangrove Forests ◽  
Acid Pretreatment ◽  
Swelling Degree ◽  
Collagen Protein

The horn snail (Telescopium telescopium) is a gastropod living in mangrove forests and is considered as a pond pest. This study was aimed to determine the effects of alkaline and acetic acid pretreatment on the characteristics of collagen from the horn snail meat. The immersion of the meat in NaOH 0.20% for 10 hours gave a significant reduction in non-collagen protein content (α = 0.05). Further immersion with acetic acid 0.05% for 6 hours also significantly affected (α = 0.05) the swelling degree and the collagen solubility. The extraction yield was 1.08±0.21%, with 72.23% of brightness, and the viscosity was 16.34 cP. The proline content of the collagen was 9.21%.

scholarly journals Effectiveness of Alkaline Pretreatment and Acetic Acid Hydrolysis on the Characteristics of Collagen from Fish Skin of Snakehead

2015 ◽  
Vol 18 (3) ◽  
Author(s):  
Wulandari Wulandari ◽  
Pipih Suptijah
Keyword(s):  
Acetic Acid ◽  
Alkaline Pretreatment ◽  
Fish Skin ◽  
Acid Pretreatment ◽  
Alternative Source ◽  
Randomized Design ◽  
Collagen Protein ◽  
Sheet Structure ◽  
Completely Randomized Design ◽  
Β Sheet

<p>Fish skin is one of marine byproducts potential for alternative source of collagen. This study<br />investigated the effectiveness of alkaline and acetic acid pretreatment on the characteristics of<br />collagen from skin snakehead fish. The concentrations of alkaline pretreatment were 0.05; 0.1; 0.15<br />and 0.2 M for 2, 4, 6, 8, 10 and 12 hours, acetic acid concentrations were 0.05 M, 0.1 M, 0.15 M and<br />0.2 M for 1 and 2 hours. The experimental design used for alkaline and acetic acid pretreatment<br />was factorial completely randomized design. The result showed that the concentration of alkaline<br />0.05 M for 6 hours have significant effect on the elimination of non-collagen protein (p&lt;0.05),<br />whereas for the optimum acetic acid at a concentration 0.1 M for 2 hours significantly different<br />on solubility and swelling. Extraction yields of collagen was 16%, with characteristics of whiteness<br />66.67%, protein content 96.21%, viscosity 10 cP, Tmax 159.9oC and glass transition temperature<br />78.55oC. The dominant amino acid composition were glycine (27.11%), proline (13.87%) and<br />alanine (12.58%). Functional groups collagen from skin snakehead fish has β-sheet structure<br />which is a characteristic of collagen.</p>

scholarly journals Pengaruh Umur terhadap Kualitas Gelatin Tulang Kaki Kambing melalui Pretreatment Asam Asetat (CH3COOH) (Effect of Age on Gelatin Quality of Goat Bones through Pretreatment of Acetic Acid (CH3COOH))

2020 ◽  
Vol 8 (2) ◽  
pp. 85-90
Author(s):  
Hasma - Hasma ◽  
Effendi Abustam ◽  
Ratmawati Malaka ◽  
Muhammad Irfan Said ◽  
Rifqi Rifqi
Keyword(s):  
Acetic Acid ◽  
Protein Content ◽  
Water Content ◽  
Ash Content ◽  
Acid Pretreatment ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Effect Of Age ◽  
Test Treatment

The purpose of this study was to determine the effect of the slaughtering age of goats on goat bone gelatin quality. The study was conducted using a completely randomized design (CRD). Data showing influence are further analyzed using the Duncan test. Treatment consisted of age-old 1-year goat bone (T1), 2-year-old goat bone (T2), and 3-year-old goat bone (T3). Each treatment was repeated 3 times. The results showed that the younger the goat age (1 year), the higher the value of yield, protein content, water content, yellowish color level (b), and viscosity. While the fat content, ash content, the level of brightness (L), and redness (a) in the colors show variable results. Each parameter showed a significant effect (P <0.05). Conclusion, 1-year-old goat leg bone gelatin with acetic acid pretreatment (CH3COOH) concentration of 5% has good quality compared to ages 2 and 3 years.

DO WEAK OR STRONG ACIDS REMOVE CARBONATE CONTAMINATION FROM ANCIENT TOOTH ENAMEL MORE EFFECTIVELY? THE EFFECT OF ACID PRETREATMENT ON RADIOCARBON AND δ13C ANALYSES

Radiocarbon ◽  
2021 ◽  
pp. 1-18
Author(s):  
Rachel Wood ◽  
Andre Barros Curado Fleury ◽  
Stewart Fallon ◽  
Thi Mai Huong Nguyen ◽  
Anh Tuan Nguyen
Keyword(s):  
Acetic Acid ◽  
Chelating Agents ◽  
Tooth Enamel ◽  
Mineral Phase ◽  
Acid Pretreatment ◽  
Skeletal Material ◽  
Lower Porosity ◽  
Hot Environments ◽  
Strong Acids

ABSTRACT In hot environments, collagen, which is normally targeted when radiocarbon (14C) dating bone, rapidly degrades. With little other skeletal material suitable for 14C dating, it can be impossible to obtain dates directly on skeletal materials. A small amount of carbonate occurs in hydroxyapatite, the mineral phase of bone and tooth enamel, and has been used as an alternative to collagen. Unfortunately, the mineral phase is often heavily contaminated with exogenous carbonate causing 14C dates to underestimate the true age of a sample. Although tooth enamel, with its larger, more stable crystals and lower porosity, is likely to be more robust to diagenesis than bone, little work has been undertaken to investigate how exogenous carbonate can be effectively removed prior to 14C dating. Typically, acid is used to dissolve calcite and etch the surface of the enamel, but it is unclear which acid is most effective. This study repeats and extends earlier work using a wider range of samples and acids and chelating agents (hydrochloric, lactic, acetic and propionic acids, and EDTA). We find that weaker acids remove carbonate contaminants more effectively than stronger acids, and acetic acid is the most effective. However, accurate dates cannot always be obtained.

scholarly journals Kinetics of Acetic Acid Pretreatment Catalyzed by Fe(NO3)3 of Corn Stover

2015 ◽  
Vol 27 (1) ◽  
pp. 185-188
Author(s):  
Xiaoyan Yu ◽  
Jun Qi ◽  
Shuting Zhang ◽  
Xuebin Lu
Keyword(s):  
Acetic Acid ◽  
Corn Stover ◽  
Acid Pretreatment ◽  
Kinetics Of

scholarly journals Impact of Alkaline Pretreatment to Enhance Volatile Fatty Acids (VFAs) Production from Rice Husk

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Qian Fang ◽  
Sinmin Ji ◽  
Dingwu Huang ◽  
Zhouyue Huang ◽  
Zilong Huang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Butyric Acid ◽  
Rice Husk ◽  
Volatile Fatty Acids ◽  
Alkaline Pretreatment ◽  
Rice Husks ◽  
Acid Fermentation ◽  
Study Results

This study explores the use of alkaline pretreatments to improve the hydrolyzation of rice husks to produce volatile fatty acids (VFAs). The study investigated the effects of reagent concentration and pretreatment time on protein, carbohydrates, and dissolved chemical oxygen demand (SCOD) dissolution after the pretreatment. The optimum alkaline pretreatment conditions were 0.30 g NaOH (g VS)−1, with a reaction time of 48 h. The experimental results show that when comparing the total VFA (TVFA) yields from the alkaline-pretreated risk husk with those from the untreated rice husk, over 14 d and 2 d, the maximum value reached 1237.7 and 716.0 mg·L−1 with acetic acid and propionic acid and with acetic acid and butyric acid, respectively. After the alkaline pretreatment, TVFAs increased by 72.9%; VFA accumulation grew over time. The study found that alkaline pretreatment can improve VFA yields from rice husks and transform butyric acid fermentation into propionic acid fermentation. The study results can provide guidelines to support the comprehensive utilization of rice husk and waste treatment.

Optimization of dilute acetic acid pretreatment of mixed fruit waste for increased methane production

2018 ◽  
Vol 190 ◽  
pp. 411-421 ◽  
Author(s):  
Shouvik Saha ◽  
Byong-Hun Jeon ◽  
Mayur B. Kurade ◽  
Shekhar B. Jadhav ◽  
Pradip K. Chatterjee ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Methane Production ◽  
Acid Pretreatment ◽  
Fruit Waste

The Progressive Decline in Diabetic Skin Integrity is Associated With Decreased Collagen Protein Content and Dysregulation of MicroRNA-29a

2012 ◽  
Vol 172 (2) ◽  
pp. 208
Author(s):  
E.J. Smith ◽  
M. Urencio ◽  
J. Xu ◽  
R.C. Caskey ◽  
M.W. Morris ◽  
...  
Keyword(s):  
Protein Content ◽  
Progressive Decline ◽  
Collagen Protein ◽  
Skin Integrity

Acetic acid pretreatment improves the hardness of cooked potato slices

2017 ◽  
Vol 228 ◽  
pp. 204-210 ◽  
Author(s):  
Wenlin Zhao ◽  
Hussain Shehzad ◽  
Shoulei Yan ◽  
Jie Li ◽  
Qingzhang Wang
Keyword(s):  
Acetic Acid ◽  
Acid Pretreatment ◽  
Potato Slices

scholarly journals Croatian produced unifloral honey characterized according to the protein and proline content and enzyme activities

2016 ◽  
Vol 60 (1) ◽  
pp. 39-48 ◽  
Author(s):  
Ivana Flanjak ◽  
Ivica Strelec ◽  
Daniela Kenjerić ◽  
Ljiljana Primorac
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Protein Content ◽  
Glucose Oxidase ◽  
Phosphatase Activity ◽  
Enzyme Activities ◽  
Acid Phosphatase Activity ◽  
Black Locust ◽  
Proline Content ◽  
A Minor

Abstract In honey, the content of proteins, including the enzymes, is relatively low and has a minor nutritive significance. On the other hand, the proteins, including the enzymes, are usually used as honey quality evaluation parameters. This is because protein content and enzyme activities vary regarding the botanical origin of the honey. Since the results of protein content, glucose-oxidase, and acid phosphatase, for honeys produced in Croatia, are not available, four of the most abundant honey types produced in Croatia (black locust, sage, chestnut, and honeydew honey) are characterised according to the protein and proline content and enzyme activities. The characterisation was done to determine specificities and contribute to the characterisation of unifloral honeys. Dark honey types (honeydew and chestnut honey) had a higher proline content, and diastase, invertase, and glucose-oxidase activity than lighter sage and black locust honey. Black locust honey has a naturally low enzyme activity and showed the highest acid phosphatase activity among the analysed honey types, while honeydew honey, otherwise known to possess high proline content and enzyme activity, had a low protein content comparable to black locust honey. Statistically significant correlations were obtained between all analysed parameters, with the exception of acid phosphatase activity.

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