scholarly journals Profiling of Collagens from Swim Bladder of Catfish (Pangasius sp.) by Acid Extraction

2019 ◽  
Vol 22 (2) ◽  
pp. 327-339
Author(s):  
Gressty Sari Br Sitepu ◽  
Joko Santoso ◽  
Wini Trilaksani
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Triple Helix ◽  
Extraction Process ◽  
Raw Material ◽  
Acid Extraction ◽  
Swim Bladder ◽  
Acid Pretreatment ◽  
Randomized Design ◽  
Completely Randomized Design

Swim bladders of catfish contain high protein, therefore it is can be used as a raw material for collagen. The study aims to determine the chemical characteristics of swim bladders, the pretreatment of non-collagen, extraction of collagen dissolves acid and to evaluate the characteristics of collagen. The method of this study is KOH pretreatment with a concentration of 0,05; 0,1; and 0,15 for 12 hours. The extraction process is done by soaking the sample in a solution of acetic acid with a concentration of 0,25; 0,5 and 0,75 M (ratio 1:10; b/v) and extraction time for 24; 48; and 72 hours at 4oC. The experimental design used for alkaline and acetic acid pretreatment were factorial completely randomized design. The result showed that the protein content of swim bladder was 85,26% (db), the profile of amino acids were dominated by three amino acids namely glycine (56,85 mg/g), prolyne (31,03 mg/g), and alanyne (23,85 mg/g). Using 0,05 M KOH for 8 h was selected as the best pretreatment method for collagen extraction. Extraction method using 0,50 M acetic for 48 h resulted he best collagen which revealed the existence of a triple helix structure and had Tg 84oC.

scholarly journals Effectiveness of Alkaline Pretreatment and Acetic Acid Hydrolysis on the Characteristics of Collagen from Fish Skin of Snakehead

2015 ◽  
Vol 18 (3) ◽  
Author(s):  
Wulandari Wulandari ◽  
Pipih Suptijah
Keyword(s):  
Acetic Acid ◽  
Alkaline Pretreatment ◽  
Fish Skin ◽  
Acid Pretreatment ◽  
Alternative Source ◽  
Randomized Design ◽  
Collagen Protein ◽  
Sheet Structure ◽  
Completely Randomized Design ◽  
Β Sheet

<p>Fish skin is one of marine byproducts potential for alternative source of collagen. This study<br />investigated the effectiveness of alkaline and acetic acid pretreatment on the characteristics of<br />collagen from skin snakehead fish. The concentrations of alkaline pretreatment were 0.05; 0.1; 0.15<br />and 0.2 M for 2, 4, 6, 8, 10 and 12 hours, acetic acid concentrations were 0.05 M, 0.1 M, 0.15 M and<br />0.2 M for 1 and 2 hours. The experimental design used for alkaline and acetic acid pretreatment<br />was factorial completely randomized design. The result showed that the concentration of alkaline<br />0.05 M for 6 hours have significant effect on the elimination of non-collagen protein (p&lt;0.05),<br />whereas for the optimum acetic acid at a concentration 0.1 M for 2 hours significantly different<br />on solubility and swelling. Extraction yields of collagen was 16%, with characteristics of whiteness<br />66.67%, protein content 96.21%, viscosity 10 cP, Tmax 159.9oC and glass transition temperature<br />78.55oC. The dominant amino acid composition were glycine (27.11%), proline (13.87%) and<br />alanine (12.58%). Functional groups collagen from skin snakehead fish has β-sheet structure<br />which is a characteristic of collagen.</p>

scholarly journals THE HISTOLOGICAL, EXTRACTION AND CHARACTERIZATION COLLAGENS YELLOW-PIKE CONGER Muarenesox talabon

2018 ◽  
Vol 9 (2) ◽  
pp. 665-683 ◽  
Author(s):  
Dewi Setiyowati Gadi ◽  
Wini Trilaksani ◽  
Tati Nurhayati
Keyword(s):  
Heavy Metals ◽  
Thermal Stability ◽  
Amino Acids ◽  
Type I Collagen ◽  
Raw Material ◽  
Acid Extraction ◽  
Type I ◽  
Swim Bladder ◽  
Molecular Weights ◽  
Acid Soluble Collagen

By product Muarenesox talabon swim bladders can be used as a raw material for desperately needed in the food, biomedical, pharmaceutical, and cosmeceuticals industries. The aims of the research were to observed the histological and determine chemical characteristics of swim bladder including proximate and amino acids; extraction of acid soluble collagen and determine the characteristics of collagen including proximate, pH, heavy metals, microbial, amino acids, functional groups, molecular weights, and thermal stability. The morphology of cunang swim bladder consists of outer, middle, and inner layers containing collagen fibers; 33.67±0.71%wb and protein whichwere dominated by three amino acids that were glycine, proline, and alanine. Pretreatment by 0.1 M NaOH for 8 hours (K1T4) and acid extraction by 0.25 M acetic acid for 72 hours (M1T3) was the best treatment yielding 14.51± 0.43% of collagen; having 12.12±0.04% wb of moisture; 88.54 ± 0.08% wb of protein; 1.31±0.23% wb of fat; 0.17±0.03% wb of ash. Not detected any heavy metals (Pb, Hg, As, Cd). Acidity pH was 4.31 and negative of E. coli and Salmonella. The main amino acids detected were glycine 241.06 mg/g; proline 88.73mg/g; and alanine 86,98 mg/g; FTIR spectra were revealed the presence of triple helix structures; electrophoresis patterns consisted of 136 kDa of mol weight of α1 and 117 kDa of mol weight of α2 were characterisedto be type I collagen; which had Tmax of 195.59ºC and ΔH 7.8113 J/g. Keywords: acid extraction, swim bladder, collagens, thermal stability

scholarly journals Pengaruh Umur terhadap Kualitas Gelatin Tulang Kaki Kambing melalui Pretreatment Asam Asetat (CH3COOH) (Effect of Age on Gelatin Quality of Goat Bones through Pretreatment of Acetic Acid (CH3COOH))

2020 ◽  
Vol 8 (2) ◽  
pp. 85-90
Author(s):  
Hasma - Hasma ◽  
Effendi Abustam ◽  
Ratmawati Malaka ◽  
Muhammad Irfan Said ◽  
Rifqi Rifqi
Keyword(s):  
Acetic Acid ◽  
Protein Content ◽  
Water Content ◽  
Ash Content ◽  
Acid Pretreatment ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Effect Of Age ◽  
Test Treatment

The purpose of this study was to determine the effect of the slaughtering age of goats on goat bone gelatin quality. The study was conducted using a completely randomized design (CRD). Data showing influence are further analyzed using the Duncan test. Treatment consisted of age-old 1-year goat bone (T1), 2-year-old goat bone (T2), and 3-year-old goat bone (T3). Each treatment was repeated 3 times. The results showed that the younger the goat age (1 year), the higher the value of yield, protein content, water content, yellowish color level (b), and viscosity. While the fat content, ash content, the level of brightness (L), and redness (a) in the colors show variable results. Each parameter showed a significant effect (P <0.05). Conclusion, 1-year-old goat leg bone gelatin with acetic acid pretreatment (CH3COOH) concentration of 5% has good quality compared to ages 2 and 3 years.

scholarly journals Performance, gut morphometry and enzymatic activity of broilers fed neonatal supplementation in housing

2018 ◽  
Vol 40 (1) ◽  
pp. 38423
Author(s):  
Tatiane Souza dos Santos ◽  
Adriano Barbieri ◽  
Robert Guaracy Aparecido Cardoso Araujo ◽  
Gustavo Do Valle Polycarpo ◽  
Daniela Felipe Pinheiro ◽  
...  
Keyword(s):  
Amino Acids ◽  
Polynomial Regression ◽  
Pancreatic Enzyme ◽  
Broiler Chicks ◽  
Quadratic Effect ◽  
Linear Effect ◽  
Intestinal Length ◽  
Randomized Design ◽  
Relative Organ Weight ◽  
Completely Randomized Design

The objective was to evaluate the performance, relative organ weight, morphometry, intestinal length and pancreatic enzyme activity of broilers receiving neonatal supplementation. A total of 900 Cobb 500 broiler chicks was housed in 30 boxes of 2.5 m². The treatments were divided in a completely randomized design with six replications, and consisted of five inclusion levels (0.0, 2.5, 5.0, 7.5 and 10 grams per bird). The supplement used was based of amino acids, vitamins and minerals, which was provided on the ration on the first day in the housing. Statistical analysis was performed using SAS (2008) and when significant broken down using a polynomial regression. Due to the inclusion of the neonatal supplement in the period from one to seven and one to 21 days of age, the feed intake presented a linear effect and the weight gain, a quadratic effect. The relative weights of the gizzard and liver showed a quadratic effect, and the inclusions of 4.18 and 3.41 grams/bird provided the lowest weights, respectively. The jejunum villi height decreased with supplementation as well as the activity of pancreatic lipase. It can be concluded that neonatal supplementation benefited the performance of birds up to 21 days of age.

scholarly journals Soybean waste in particleboard production

2018 ◽  
Vol 42 (2) ◽  
pp. 186-194 ◽  
Author(s):  
Evelyn Hoffmamm Martins ◽  
Alan Pereira Vilela ◽  
Rafael Farinassi Mendes ◽  
Lourival Marin Mendes ◽  
Lívia Elisabeth Vasconcellos de Siqueira Brandão Vaz ◽  
...  
Keyword(s):  
Water Absorption ◽  
Water Immersion ◽  
Eucalyptus Grandis ◽  
Raw Material ◽  
Modulus Of Rupture ◽  
High Yield ◽  
Thickness Swelling ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Hybrid Eucalyptus

ABSTRACT Brazil is the second largest soybean producer in the world, with a yield of around 96.2 million tons per crop. This high yield leads to a great amount of waste resulting from soybean cultivation, which can reach approximately 41 million tons of waste per year. This material has lignocellulosic properties, which may enable its use as a raw material for particleboard production. Therefore, the objective of this study was to evaluate the use of soybean pods in particleboard production. For particleboard manufacture, wood of the hybrid Eucalyptus urophylla and Eucalyptus grandis was used, added with soybean pods, at proportions of 0%, 25%, 50%, 75% and 100%. For particleboard evaluation, a completely randomized design was used, with five treatments and three replicates, using linear regression and the Scott-Knott test at 5% significance for comparison among the different treatments. The properties apparent density, compaction ratio, water absorption after 2 and 24 hours, thickness swelling after 2 and 24 hours in water immersion, internal bonding, modulus of rupture and modulus of elasticity in bending properties were evaluated. The ratio soybean pod waste and eucalyptus particles in the panels led to an increase in water absorption values and thickness swelling, in addition to a decrease in mechanical properties. The production of panels with approximately 23% soybean pods is feasible.

scholarly journals Isolation and Charactherization of Collagen from Swim Bladder Waste of Yellow-pike Conger (Muraenesox talabon)

2016 ◽  
Vol 19 (3) ◽  
pp. 222 ◽  
Author(s):  
I Wayan Darya Kartika ◽  
Wini Trilaksani
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Physicochemical Properties ◽  
Functional Groups ◽  
Hydrothermal Process ◽  
Acid Extraction ◽  
Swim Bladder ◽  
Chemical Content ◽  
Wet Weight ◽  
Acid Process

<p>Indonesian capture fisheries production reached ± 6.4 million tons in 2014. This number was predicted<br />resulted a huge fishery waste, one of them was swim bladder that potentially contain collagen, so it needs to<br />be developed. This aim of this study was to determine the characteristics of the swim bladder (proportion<br />and chemical content) and collagen characteristics (yield and physicochemical properties) extracted from<br />the swim bladder cunang (Muraenesox talabon). Swim bladder waste had proportion 0.57 to 0.67% of whole<br />yellow-piked conger body weight and 24.74% protein/100 g wet weight (equivalent to 93.39% of protein/100<br />gram dry). The waste had a potency of collagen, which is characterized by the high proportion of the<br />amino acids glycine, proline, hydroxyproline and alain. Acid extraction and hydrothermal process resulted<br />collagen extracts, identified from a spectrum of amide I, amide II and amide III functional groups. Collagen<br />extracted with acid process contained a higher glycine than hydrothermal one, but instead hydrothermal<br />process produced extract with a higher proline, hydroxyproline and alanine composition.<br /><br /></p>

scholarly journals POTENSI KULIT KOPI SEBAGAI BAHAN BAKU PUPUK KOMPOS DI PROPINSI BENGKULU

2016 ◽  
Vol 2 (2) ◽  
Author(s):  
Afrizon
Keyword(s):  
Rice Bran ◽  
Secondary Data ◽  
Raw Material ◽  
Skin Potential ◽  
Coffee Waste ◽  
Randomized Design ◽  
Leather Wastes ◽  
Completely Randomized Design ◽  
Leather Waste ◽  
Fruit Harvest

The experimen of Coffee For Skin Potential Raw Material Compost in Bengkulu Province was conducted from March to June 2010 in the laboratory greenhouse BPTP Bengkulu. The purpose of this study are 1) Testing coffee leather waste into compost in an effort to preserve the environment, 2) Determine the potential of waste as raw material for leather coffee fertilizer in Bengkulu Province. In the manufacture of compost using a completely randomized design (CRD) with 6 treatments and 3 replications. The treatments were: a) 70% waste + 20% KDP coffee cage + 10% rice bran + EM4 + sugar, b) 80% waste + 10% KDP coffee cage + 10% rice bran + EM4 + sugar, c) 80% waste coffee + 20% KDP cage + EM4 + sugar, d) 80% + 20% of coffee waste Manure + EM4, e) 80% waste + 10% KDP coffee cage + 10% rice bran, f) 80% waste + 20 copies % KDP cage. As for seeing the potential of coffee leather waste by gathering primary and secondary data related to commodities which includes the area planted with coffee and productivity in every district in Bengkulu province. The data collected is the percentage of skin waste of coffee per 100 g, area planted with coffee every district, the content of N, P, K and C organic. The research shows that compost with time for 4 weeks has given the maturity of fertilizer as indicated by the value of C / N between 9.75 to 15.99. The composition of 80% waste + 10% coffee hull Manure + 10% rice bran provides the best compost. Compost made from leather wastes contain nutrients coffee in accordance with ISO standards 19-7030-2004 about compost quality standards. Dried coffee fruit harvest yield 51.2% milled coffee leather waste with a potential of 30,222 tons / year. equivalent to 738.323 tons N / yr. This is identical to substitute with an equal amount of urea (46% N) of 339.628 tons / year. equivalent to 86.434 tons P205/th. This is identical to substitute with an equal value superphos (SP-36) amounted to 31.116 tons / yr, equivalent to 876.438 kg K2O/th or identical substitute KCl (60%) amounted to 525.862tons/year.  Keywords : coffee leather waste, compost, potential

scholarly journals LIMBAH TERNAK KELINCI SEBAGAI BAHAN BAKU PUPUK ORGANIK POTENSIAL

2018 ◽  
Vol 16 (2) ◽  
pp. 152
Author(s):  
Risvan Anwar ◽  
Djatmiko Djatmiko
Keyword(s):  
Raw Materials ◽  
Organic Fertilizer ◽  
Nutrient Content ◽  
Raw Material ◽  
Organic Fertilizers ◽  
Animal Waste ◽  
Livestock Waste ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Many Sources

Many sources of raw materials which allegedly can enrich the nutrient content in the organic fertilizer. The weakness of organic fertilizer for this is the low level of nutrients contained in them.This study aims to determine the nutrient content contained in various combinations of organic fertilizer raw materials with rabbit animal waste as the main raw material.This study aims to determine the nutrient content in various combinations of organic fertilizer raw materials with rabbit animal waste as the main raw material. The experiment used a completely randomized design with raw materials as treatments, Livestock Waste Rabbit (LTK), Cow Manure (PKS) and Abu Straw Rice (AJP). Such treatment: B1 = LTK; B2 = LTK: PKS = 2: 1; B3 = LTK: AJP = 3: 1; B4 = LTK: PKS: AJP = 6: 2: 1; B5 = LTK: PKS: AJP = 5: 2: 1; B6 = LTK: PKS: AJP = 4: 2: 1; B7 = LTK: PKS: AJP = 3: 2: 1; B8 = LTK: PKS: AJP = 2: 2: 1; B9 = LTK: PKS: AJP = 1: 2: 1. The research concluded (a) Organic fertilizers are made from various materials raw has fulfilled SNI 19-7030-2004 about the specifications of Organic Waste Compost. (B) A combination of organic fertilizer raw materials significantly affect the nutrient content of manure. (C) Organic fertilizers expectations are: (a) Raw materials of animal wastes rabbit (LTK), (b) LTK: PKS: AJP = 6: 2: 1, (c) LTK: PKS: AJP = 2: 2: 1 and (d) LTK: PKS: AJP = 1: 2: 1.

scholarly journals Edible coating quality with three types of starch and sorbitol plasticizer

2020 ◽  
Vol 142 ◽  
pp. 02003 ◽  
Author(s):  
Retno Utami Hatmi ◽  
Erni Apriyati ◽  
Nurdeana Cahyaningrum
Keyword(s):  
Tensile Strength ◽  
Water Vapor ◽  
Raw Materials ◽  
Chemical Properties ◽  
Edible Coating ◽  
Raw Material ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Arrowroot Starch

Edible coating is one form of packaging technology with environmentally friendly theme. The raw materials of edible coating derived from nature, while the waste is decomposed or even zero waste. The research of edible coating using experimental design RAL (completely randomized design) with two factors, namely the type of raw material used tuber starch (cassava, arrowroot and canna) and the percentage of starch (3%, 4% and 5%) (b/v) with three replications time. The quality analisys of edible coating includes the physical properties (thickness (mm), tensile strength (N) and elongation (mm)) and chemical properties (moisture content (%), solubility (%), the water vapor transmissin rate (g/hour) and peroxide (mek/kg). The research showed that the edible coating with sorbitol plasticizer of arrowroot starch 4% provide best physicochemical properties (thickness 0,09mm; 1,63N tensile strength; elongation 84,38mm; water content of 11.19%; solubility of 31.40%; the transfer of water vapor 0,16g / h and 3,20mek/ kg).

scholarly journals FERMENTASI BIJI KECIPIR (Psophocarpus tetragonolobus) OLEH JAMUR TRICHODERMA VIRIDE TERHADAP WARNA, TEKSTUR, DAN SERAT KASAR

2019 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Catur Suci Purwati ◽  
Ludfia Windyasmara
Keyword(s):  
Fermentation Process ◽  
Trichoderma Viride ◽  
Raw Material ◽  
Crude Fiber ◽  
Psophocarpus Tetragonolobus ◽  
Nutrient Quality ◽  
Randomized Design ◽  
Plastic Bag ◽  
Completely Randomized Design ◽  
Better Than

The fermentation process is an activity of microorganisms that make products with characteristics of texture, flavor, aroma and changes in nutrient quality that better than the original raw material. It is also protein process of developing of protein from the material. This study uses a Completely Randomized Design with four treatments. P0 = Unfermented winged seeds, P1 = Winged seeds + 0,1% Trichoderma viride mushroom (Tv), P2 = Winged seeds + 0,2% Trichoderma viride mushroom (Tv), P3 = Winged seeds + 0,3% Trichoderma viride mushroom (Tv). The material used was crushed winged seeds as substrates that mixed to be homogenized. One hundred gr of each sample from every treatment was weighed, put into a perforated plastic bag (to create anaerobic atmosphere) with 2 cm thick. Subsequently, samples were incubated in fermented room at 30oC for 7 days. Each treatment was repeated 3 times. Variables observed in color, texture and coarse fiber. This study indicated that the winged fermented beans with Trichoderma viride mushroom had no effect on color, effected crude fiber and texture.

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