scholarly journals Acid and calcium alginate extraction method affected the quality of alginate from brown seaweed Sargasum hystrix J. Agardh

2018 ◽  
Vol 21 (3) ◽  
pp. 532
Author(s):  
Ivana M. Diharningrum ◽  
Amir Husni
Keyword(s):  
Moisture Content ◽  
Sodium Alginate ◽  
Extraction Method ◽  
Calcium Alginate ◽  
Functional Group ◽  
Brown Seaweed ◽  
Extraction Methods ◽  
Ash Content ◽  
Alginate Extraction

Seaweed is one of the marine biological resources of Indonesia that has a high potential to produce alginate, however, the most appropiate method to extract the alginate of high quality is still unknown. This study was aimed to determine the effects of extraction methods on the quality of alginate from seaweed Sargassum hystrix and compared the extraction cost. Two extraction methods were used in this study including acidic and calcium method. The yield, moisture content, viscosity, pH, whiteness degree, functional group, ash content and extraction cost were evaluated. The viscosity and whiteness degree of sodium alginate from acidic method was 126.00±7.21 cPs and 75.27±0.58 cPs, respectively. Meanwhile the calcium method produced sodium alginate with viscosity 7.23±1.50 cPs and whiteness degree 68.95±0.18 cPs. The yield, moisture content, and ash content of alginate produced by acidic method were lower compare to that of produced by calcium method. Cost analysis showed the acidic method required higher cost. Nevertheless, these resultssuggest that acidic method produce alginate with better quality than the calcium method.

scholarly journals Effect of Extraction Methods on Characteristic of SodiumAlginate from Brown Seaweed Sargassum fluitans

2018 ◽  
Vol 20 (3) ◽  
pp. 478 ◽  
Author(s):  
Annisa Ajeng Maharani ◽  
Amir Husni ◽  
Nurfitri Ekantari
Keyword(s):  
Sodium Alginate ◽  
Alginic Acid ◽  
Group Analysis ◽  
Brown Seaweed ◽  
Extraction Methods ◽  
Quality Parameters ◽  
Ash Content ◽  
Primary Metabolite ◽  
Acid Method ◽  
Alginate Extraction

Alginate is a primary metabolite that is needed in food and non food industries. Alginate extraction<br />method of seaweed affect on viscosity and yield of the alginate. This study aimed to determine the effect of<br />extraction methods on characteristic and quality of sodium alginate Sargassum fluitans and the extraction<br />cost needed. This study used two different extraction methods which wereacid alginate method and calcium<br />alginate method. Quality parameters observed include yield, moisture content, ash content, viscosity,<br />pH, whiteness index, and functional group analysis, also extraction cost analysis. The results showed that<br />alginate extraction of S. fluitans by alginic acid method produced better sodium alginate quality than using<br />calcium alginate method, but the cost higher. Sodium alginate produced by alginic acid method had high<br />viscosity (127.17±11.50 cps) with yield 9.95±0.31%. The moisture and ash content of the product was low or<br />9.35±0.31% and 21.88±0.41%, respectively.<br /><br />

scholarly journals The Characteristics of Sodium Alginate from Brown Seaweed Sargassum crassifolium with Different Filtering Tools

2017 ◽  
Vol 20 (2) ◽  
pp. 351 ◽  
Author(s):  
Ellya Sinurat ◽  
Retni Marliani
Keyword(s):  
Moisture Content ◽  
Sodium Alginate ◽  
Brown Seaweed ◽  
Gel Strength ◽  
Filter Press ◽  
Average Yield ◽  
The World ◽  
Filtration Stage ◽  
Alginate Extraction

Indonesia is the largest producer of seaweed in the world, one of the potential marine biological resources is the brown seaweed (Sargassum crassifolium) known as an alginate producer. Alginate extraction through demineralization stage, neutralization, extraction, filtration, precipitation and bleaching. Filtration stage is a very influential process on the quality of alginate produced. In this research, filtering using different tools screen vibrator and hydraulic filter press was conducted to characterize Na-alginate seaweed Sargassum crassifolium using both filter tools. Quality of sodium alginate (yield, viscosity, gel strength, syneresis, whiteness, moisture content, ash, and pH) was determined. The result showed that the viscosity, gel strength, syneresis, and whiteness of alginate obtained using filter press showed the best result. The yield of sodium alginate produced by filter press was 10.91±4.33%, with characteristics of viscosity 82.66±112.46 cP, gel strength 353.54±184.51 g/cm2, syneresis 2.99±0.55%, whiteness 60.53±9.09%, moisture content 13.31±0.77%, ash 26.69±0.82% and pH 6.05±0.57.  Based on a parameter of alginate (yield, moisture content, ash, and pH) showed that vibrator method produced alginate with better characteristics. The sodium alginate produced has average yield 19.22±5.68%, viscosity 57.5±21.79 cP, whiteness 29.7±4.45%, gel strength 327.63±55.15 g/cm2, syneresis 4.34±0.78%, moisture content 10.23±1.68%, ash 24.94±4.41% and pH 7.03± 2.60. Operationally the filter press was more effective and efficient in filtering to result from Na-alginate compared to the screen vibrator.

scholarly journals Optimization of Extraction Temperature on The Quality of Alginate from Seaweed Sargassum muticum

2019 ◽  
Vol 22 (1) ◽  
pp. 164
Author(s):  
Rizqi Wahyu Herdianto ◽  
Amir Husni
Keyword(s):  
Moisture Content ◽  
Sodium Alginate ◽  
Ash Content ◽  
Sargassum Muticum ◽  
Extraction Temperature ◽  
Food Industries ◽  
The Difference ◽  
Effect On Yield ◽  
Alginate Extraction

Seaweed is one of Indonesia’s marine biological resources which has potential to produce alginate which can be used for food and non-food industries. Several studies had been carried out in an effort to improve the quality of alginate extraction. This study aims to determine the effect of extraction temperature on the quality of sodium alginate from Sargassum muticum. This study used variations in extraction temperatures, namely 30; 40; 50; 60 and 70°C. The parameters observed included yield (%), moisture content (%), ash content (%), pH, whiteness degree and functional groups of alginate. The results showed that the difference in extraction<br />temperature of sodium alginate had a significant effect on yield and viscosity, but did not significantly affect water content, ash content, and whiteness degree. Extraction temperature of 60°C produced the best yield<br />and viscosity, 14.90±2.26% and 236.10±36.61 cPs, respectively.

scholarly journals RASIO NATRIUM KARBONAT DALAM EKSTRAKSI BERPENGARUH PADA MUTU NATRIUM ALGINAT Sargassum muticum

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Sovia Indah Nurkhanifah ◽  
Amir Husni
Keyword(s):  
Moisture Content ◽  
Functional Group ◽  
Extraction Efficiency ◽  
Quality Standards ◽  
Ash Content ◽  
Sargassum Muticum ◽  
Quality Analysis ◽  
Lower Viscosity ◽  
Alginate Extraction

Several studies have been conducted in an effort to improve alginate extraction efficiency. This study aims to determine the effect of Na2CO3 ratio on the quality of sodium alginate Sargassum muticum. The Na2CO3 ratios used were 1:10, 1:20, 1:30, 1:40, 1:50 (w/v) and quality analysis were made on yield (%), viscosity (cps), pH, moisture content (% ), white degree (%), ash content (%) and functional group. The results showed that the higher Na2CO3 ratio had significantly lower viscosity. The value of yield, pH, moisture content, degree of white, and ash content showed that the effect of Na2CO3 ratio did not significantly different. The best Na2CO3 ratio was 1:20 w/v because it had medium viscosity, besides that, as a result of yield, pH, moisture content, white degree, ash content has met alginate quality standards.

scholarly journals Analisis Kualitas Alginat Rumput Laut (Padina sp.) Menggunakan Metode Ekstraksi Jalur Kalsium

2020 ◽  
Vol 9 (1) ◽  
pp. 75-80
Author(s):  
Adam Sojuangon Pasaribu ◽  
Sri Sedjati ◽  
Rini Pramesti
Keyword(s):  
Brown Seaweed ◽  
Extraction Methods ◽  
Quality Parameters ◽  
Ash Content ◽  
Ftir Analysis ◽  
Test Results ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Yield Value

ABSTRAK: Padina sp. merupakan spesies rumput laut cokelat (Phaeophyta) penghasil alginat, namun kualitas alginat yang dihasilkan tergolong rendah dibandingkan alginofit lainnya. Metode ekstraksi jalur kalsium diduga dapat meningkatkan kualitas alginat sehingga penelitian ini perlu dilakukan. Tujuan penelitian ini untuk mengkaji apakah ekstraksi jalur kalsium dapat meningkatkan mutu alginat yang dihasilkan dari rumput laut Padina. Ekstraksi dilakukan dengan metode jalur kalsium konvensional dan jalur kalsium MAE (Microvawe Assisted Extraction). Metode penelitian yang digunakan adalah eksperimental laboratoris dengan 1 perlakuan (kosentrasi CaCl2) dan 3 taraf konsentrasi yaitu 0,5 M 1,0 M dan 1,5 M. Parameter kualitas yang dianalisis meliputi rendemen, kadar air, Kadar abu, viskositas, pH dan FTIR. Hasil uji two way ANOVA menunjukan terdapat signifikansi rendemen dengan nilai 0,007. Metode jalur kalsium MAE dengan konsentrasi 1,0 M CaCl2 menghasilkan kualitas terbaik dengan nilai rendemen (26,83%), kadar air (10,67%), kadar abu (21,83%), viskositas (11,42 cPs)  dan pH (6,77). Hasil analisis FTIR menunjukan ke-2 metode ekstraksi menghasilkan natrium alginat dengan adanya sidik jari guluronat dan manuronat pada bilangan gelombang 930-890 cm-1 dan 870-820 cm-1. ABSTRACT: Padina sp. is a species of brown seaweed (Phaephyta) producing alginates, but the quality of alginates produced is relatively low compared to other alginophytes. The calcium pathway extraction method is thought to improve the quality of alginates thus the reason of the research. This research aims to examine whether the calcium pathway extraction improves the quality of alginates produced from Padina. Seaweed extraction was carried out using conventional calcium pathways and MAE (Microwave Assisted Extraction) calcium pathways. The research method used was laboratory experimental with 1 treatment (CaCl2 concentration) and 3 concentration levels : 0.5 M, 1.0 M, 1.5 M. Quality parameters analyzed includes yield, moisture content, ash contetnt, viscosity, pH and FTIR. The two way ANOVA test results showed that there was a significance of yield value of 0.007. The MAE calcium pathway method with a concentration of 1.0 M CaCl2 produces the best quality with a yield value (26.83%), water content (10.67%), ash content (21.83%), viscosity (11.42 cPs) and pH (6.77). FTIR analysis results show that the two extraction methods produces sodium alginate in the presence of guluronate and manuronic fingerprints at wave numbers 930-890 cm-1 and 870-820 cm-1.

scholarly journals Pathway of sodium alginate synthesis from marine brown algae, Sargassum wightii from Sri Lanka

Discover Food ◽  
2022 ◽  
Vol 2 (1) ◽  
Author(s):  
G. D. T. M. Jayasinghe ◽  
B. K. K. K. Jinadasa ◽  
N. A. G. Sadaruwan
Keyword(s):  
Sodium Alginate ◽  
Extraction Method ◽  
Sodium Carbonate Solution ◽  
Brown Seaweed ◽  
Gel Strength ◽  
Ash Content ◽  
Main Step ◽  
Standard Methods ◽  
Sargassum Wightii ◽  
High Affinity

AbstractAlginates are natural polysaccharides that are extracted from brown seaweed varieties and it is widely used for their rheological properties. The main step in the extraction protocol of sodium alginate is alkaline extraction. Sodium alginate was produced by dipping the seaweed in 1% formaldehyde and 2.5% of sodium carbonate solution and properties were studied following the standard methods. The amount of sodium alginate yield was 31.7% in Sargassum wightii. The moisture content and the ash content were recorded at 16.82% and 5.20%, respectively. The viscosity and the gel strength were noted as 40 vcP, 4.54 × 10–2 kN with 0.1 M CaCl2 and 6.86 × 10–2 kN with 0.2 M CaCl2 respectively. This study of the extraction method and its properties reveal that Sargassum wightii brown seaweed species have a high affinity to extract the alginate.

scholarly journals PENGARUH METODE EKSTRAKSI MINYAK PUPA ULAT JERMAN TERHADAP KOMPOSISI KIMIA

BUANA SAINS ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 67
Author(s):  
Erik Priyo Santoso ◽  
Farida Kusuma Astuti
Keyword(s):  
Extraction Method ◽  
Crude Protein ◽  
Dry Matter ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Chemistry Laboratory ◽  
Research Material ◽  
Soxhlet Method ◽  
Heat Method

The aim of this research is to know the extraction method of caterpillar pupa without detrimental to chemical composition to know the amount of pupa production of German caterpillar with two extraction methods per 100 grams of pupa To know the content of dry matter, organic matter, crude protein, crude fat, crude fiber and amino acid lysin, methionine, and vitamin E pupa oil of German caterpillar. The study was conducted from February to April 2017, at the Lab. Central University Tribhuwana Tunggadewi. Oil extraction analysis was conducted in the chemistry laboratory of Tribhuwana Tunggadewi University and chemistry laboratory of Poltek Malang. German caterpillar research material, German caterpillar pupa, pupa caterpillar germany extracted by maceration and sokletasi. German caterpillar pupa is obtained from German caterpillar breeders in Amadanom, Dampit, Malang Regency. This research uses two different extraction methods, namely cold extraction method (maseration method) and using heat method (soxhlet method). The conclusions of both research methods are known that the quantity of German caterpillar yield using soxhlet extraction method is more than the maceration extraction method. However, for the quality of the two methods it is still necessary to do further analysis to know the exact comparison of the two methods.

scholarly journals Analysis of the Quality of Mixed Coconut Shell Waste Briquettes with Various Biomass Additives as Alternative Fuels

2021 ◽  
Vol 4 (2) ◽  
Author(s):  
Yusraida Khairani Dalimunthe ◽  
Sugiatmo Kasmungin ◽  
Listiana Satiawati ◽  
Thariq Madani ◽  
Teuku Ananda Rizky
Keyword(s):  
Moisture Content ◽  
Calorific Value ◽  
Volatile Matter ◽  
Matter Content ◽  
Ash Content ◽  
Coconut Shell ◽  
Bamboo Charcoal ◽  
A Value ◽  
Shell Waste

The purpose of this study was to see the best quality of briquettes from the main ingredient of coconut shell waste<br />with various biomass additives to see the calorific value, moisture content, ash content, and volatile matter<br />content of the biomass mixture. Furthermore, further research will be carried out specifically to see the quality of<br />briquettes from a mixture of coconut shell waste and sawdust. The method used in this research is to conduct a<br />literature study of various literature related to briquettes from coconut shell waste mixed with various additives<br />specifically and then look at the best quality briquettes produced from these various pieces of literature. As for<br />what is determined as the control variable of this study is coconut shell waste and as an independent variable,<br />namely coffee skin waste, rice husks, water hyacinth, Bintaro fruit, segon wood sawdust, coconut husk, durian<br />skin, bamboo charcoal, areca nut skin, and leather waste. sago with a certain composition. Furthermore, this<br />paper also describes the stages of making briquettes from coconut shell waste and sawdust for further testing of<br />the calorific value, moisture content, ash content, volatile matter content on a laboratory scale for further<br />research. From various literatures, it was found that the highest calorific value was obtained from a mixture of<br />coconut shell waste and bamboo charcoal with a value of 7110.7288 cal / gr and the lowest calorific value was<br />obtained from a mixture of coconut shell waste and sago shell waste with a value of 114 cal / gr, then for the value<br />The highest water content was obtained from a mixture of coconut shell waste and rice husk with a value of<br />37.70% and the lowest water content value was obtained from a mixture of coconut shell waste 3.80%, then for the<br />highest ash content value was obtained from a mixture of coconut shell waste and coffee skin with a value of<br />20.862% and for the lowest ash content value obtained from a mixture of coconut shell and Bintaro fruit waste,<br />namely 2%, and for the highest volatile matter content value obtained from a mixture of coconut shell and coconut<br />husk waste with a value of 33.45% and for the value of volatile matter levels The lowest was obtained from a<br />mixture of coconut shell waste and sago skin waste with a value of 33 , 45%.

scholarly journals DNA extraction technique for different rice varieties grown in Sri Lanka

2015 ◽  
Vol 3 (3) ◽  
pp. 398-401
Author(s):  
Ranganathan Kapilan
Keyword(s):  
Sri Lanka ◽  
Dna Extraction ◽  
Extraction Method ◽  
Extraction Methods ◽  
Rice Varieties ◽  
Modified Method ◽  
Dna Extraction Methods ◽  
Very High ◽  
Different Parts

Extraction of DNA is very important nowadays in bio-molecular researches. Extracted DNA should be purified and the quality of DNA should also be very high. The objective of the study was to develop a simple efficient method to isolate DNA from the rice varieties in an open laboratory environment, and to eliminate the usage of expensive chemicals and tools. The DNA extraction methods developed by the DNeasy plant kit method supplied by QIAGEN, Cheng et al., Doyle et al. and Michiels et al. were applied to five different rice varieties grown in different parts of Sri Lanka. Based on the quantity and quality of the extracted DNA tested by measuring the absorbance of DNA at 260 nm using Nanodrop® ND-1000 spectrophotometer and measuring the ratio of A260 / A280 and gel running on agarose, the efficiency of the extraction method chosen varied among rice varieties. Among the methods used, the methods introduced by DNeasy plant kit method supplied by QIAGEN and Cheng et al, yielded good and amplifiable quality DNA with satisfactory concentration for all the rice varieties tested. Therefore the modified method of Cheng et al, 1987 could be used to extract DNA from rice varieties instead of the commercially available expensive and hazardous DNeasy plant kit method supplied by QIAGEN.Int J Appl Sci Biotechnol, Vol 3(3): 398-401

scholarly journals Biosorpsi Ion Logam Tembaga (Cu2+) Pada Biosorben Rumput Laut Coklat (Padina australis)

2018 ◽  
Vol 6 (1) ◽  
pp. 26-37
Author(s):  
Catherina M. Bijang ◽  
Jolantje Latupeirissa ◽  
Marike Ratuhanrasa
Keyword(s):  
Moisture Content ◽  
Metal Ions ◽  
Atomic Absorption ◽  
Contact Time ◽  
Brown Seaweed ◽  
Ash Content ◽  
Atomic Absorption Spectrophotometer ◽  
Batch Method ◽  
Optimum Ph ◽  
Determine Moisture Content

The research on the biosorption of Cu2+ metal ions in brown seaweed biosorbent (Padina australis) has been done. This study aims to determine moisture content, ash content, biomass content, pH and optimum contact time of Cu2+  metal ions absorption pH and optimum contact time of Cu2+  metal ions absorption. The method used is batch method. The adsorption result of Cu2+ metal ions was analyzed  by  using  atomic  absorption  spectrophotometer  (AAS).  The  results showed that the optimum pH was 7 with 99.8308 % absorption ability of biosorbent and the optimum contact time was 5 hours with 99.5570 % biosorbent absorbency.

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