scholarly journals Produksi Pigmen dan Identifikasi Kapang Penghasilnya Menggunakan Pendekatan DNA Barcoding

2018 ◽  
Vol 21 (1) ◽  
pp. 99
Author(s):  
Mada Triandala Sibero ◽  
Kustiariyah Tarman ◽  
Ocky Karna Radjasa ◽  
Agus Sabdono ◽  
Agus Trianto ◽  
...  

Kapang endofit laut diketahui sebagai sumber berbagai senyawa bioaktif yang berguna bagi kesehatan. Penelitian yang telah dilakukan sebelumnya membuktikan bahwa kapang endofit isolat RS3 asal tanaman pesisir <em>Hydnophytum formicarum</em> asal Sorong menghasilkan pigmen hitam dengan potensi sebagai fotoprotektor namun pada penelitian tersebut tidak memiliki data mengenai produksi pigmen serta identifikasi kapang penghasilnya. Penelitian ini bertujuan untuk menentukan produksi pigmen hitam asal kapang RS3 dan identifikasi kapang RS3 melalui pendekatan DNA barcoding. Kapang RS3 dikultivasi selama 30 hari menggunakan sistem statis dan sistem shaking. DNA barcoding pada identifikasi kapang<br />RS3 menggunakan primer universal ITS1 dan ITS4 untuk mengamplifikasi wilayah <em>internal transcribed space</em>r (ITS) yang menjadi conserve region pada kingdom fungi. Hasil penelitian menunjukkan bahwa kapang RS3 yang dikultivasi menggunakan sistem shaking mulai memproduksi pigmen pada hari-6 dan mencapai produksi tertinggi pada hari ke-18 (2,42±0,03 mg/L) sedangkan pada kultivasi sistem statis rendemen pigmen terbesar dihasilkan pada hari ke-30 (2,88±0,21 mg/L). Berdasarkan hasil analsis ITS rDNA diketahui bahwa kapang endofit RS3 memiliki 99% kemiripan dengan Annulohypoxylon stygium. Kapang ini telah didaftarkan di GeneBank denga nomer akses  MG605083.1.<br /><br />

Author(s):  
Pham Thi Ngoc ◽  
Pham Thanh Huyen ◽  
Nguyen Quynh Nga ◽  
Phan Van Truong ◽  
Nguyen Minh Khoi ◽  
...  

Panax L. is a small genus in the Araliaceae family. In Vietnam, this genus is distributed in the high mountains in the North and in some high mountains in the Central Highlands. In traditional medicine, roots and rhizomes of all Panax species are of high utility. Recently, the finding of new distributions of some Panax species in Vietnam has caused much controversy and confusion. This study investigates 27 fresh specimens of 6 taxa of Panax genus that have been collected from 6 provinces of Vietnam. Based on the combined ITS-rDNA and matK dataset, a well-resolved phylogeny of Panax species/varieties distributed in Vietnam was constructed. Thereby, the study suggests identifying Tam that la xe as Panax stipuleanatus H.T.Tsai et K.M.Feng and Sam puxailaileng as Panax vietnamensis var. fuscidiscus K.Komatsu, S.Zhu & S.Q.Cai, which contributes a new distribution point of this variety in Vietnam. The study also shows that ITS-rDNA and matK genes are highly potential in identifying and distinguishing the taxa of Panax genus. Keywords Panax, ginseng, molecular phylogeny, taxonomy, ITS-rDNA, matK. References [1] Xiang, Q.B. & Lowry, P.P. Araliaceae, In: Wu, C.Y., Rawen, P.H. & Hong (2007).[2] Nguyen Tap, The species of Panax L. in Vietnam, Journal of Medicinal Material, 10 (3) (2005) 71-76 (in Vietnamese).[3] Phan Ke Long, Le Thanh Son, Phan Ke Loc, Vu Dinh Duy and Pham Van The, Lai Chau ginseng Panax vietnamensis var. fuscidiscus K. Komatsu, S. Zhu & S.Q. Cai.I. morphology, ecology, distribution and conservation status, Proceedings of the 2nd VAST – KAST workshop on biodiversity and bio-active compounds, Hanoi- Vietnam, October 28th – 29th (2013), pp. 65-73, Publishing house for science and technology. [4] Nong Van Duy, Le Ngoc Trieu, Nguyen Duy Chinh & Van Tien Tran, A new variety of Panax (Araliace) from Lam Vien Plateau, Vietnam and its molecular evidence, J. Phytotaxa 277(1) (2016) 047-058. http://dx.doi.org/10. 11646/ phytotaxa.277.1.4[5] Tran Ngoc Lan et al., Results of study on Sam Puxailaileng in high moutain, Nghe An province, Nghe An journal of Science and Technology 12 (2016) 7-11 (in Vietnamese). [6] Do Huy Bich et al., Medicinal plant and animal in Vietnam, ep. II (2004), Science & Technic Pub. pp. 1255 (in Vietnamese).[7] X. Chen, B. Liao, J. Song, X. Pang, J. Han, S. Chen, A fast SNP identification and analysis of intraspecific variation in the medicinal Panax species based on DNA barcoding, Gene 530(1) (2013) 39-43. http://doi.org/10.1186/j.gene.2013.07.097[8] Jun Wen and Elizabeth A. Zimmer, Phylogeny and Biogeography of Panax L. (the Ginseng Genus, Araliaceae): Inferences from ITS Sequences of Nuclear Ribosomal DNA, Molecular Phylogenetics and Evolution 6(2) (1996) 167-177.[9] C. Lee, J. Wen, Phylogeny of Panax using chloroplast trnC–trnD intergenic region and the utility of trnC–trnD in interspecific studies of plants, Mol Phylogenet Evol 31(2004): 894-903, DOI: 10.1016/j.ympev.2003.10.009[10] Y. Zuo, Z. Chen, K. Kondo, T. Funamoto, J. Wen, S. Zhou, DNA barcoding of Panax species, Planta Med. 2011 Jan, 77(2) (2011):182-187. Doi: 0.1055/s-0030-1250166.[11] K. Komatsu, S. Zhu, S.Q. Cai, A new variety of the genus Panax from Southern Yunnan, China and its nucleotide sequences of 18S ribosomal RNA gene and matK gene, J. Jap. Bot 78(2) (2003) 86-94. [12] V. Manzanilla1, A. Kool, L. Nguyen Nhat, H. Nong Van, H. Le Thi Thu and H. J. de Boe, Phylogenomics and barcoding of Panax: toward the identification of ginseng species. BMC Evolutionary Biology (2018) 18-44. http://doi.org/10.1186/s12862-018-1160-y[13] Vu Huyen Trang, Hoang Dang Hieu, Chu Hoang Ha, Study on DNA barcode for identify Sam ngoc linh, National conference of biotechnology, Ep.2, (2013) 1100-1104.[14] Nguyen Thi Phuong Trang, Nguyen Thi Hong Mai, Zhuravlev Yury N, Reunova Galina D., rbcL and rpoB gene sequences of Panax vietnamensis var. fuscidiscus and Panax vietnamensis, the background for identification and comparison, Journal of Biology 39(1) (2016) 80-85. http://doi.org/10.15625/0866-7160/v39n1.7870[15] Phan Ke Long, Tran Thi Viet Thanh, Nguyen Thien Tao, Phan Ke Loc, Nguyen Tu Lenh, Nguyen Tien Lam, Dang Xuan Minh, Morphological and molecular characsteristics of Panax sp. (Araliaceae) from Phu Xai Lai Leng mountain, Nghe An province, Vietnam, Journal of Biology 36(4) (2014) 494-499. https://doi.org/ 10.15625/0866-7160/v36n4.5212[16] Phan Ke Long, Vu Dinh Duy, Phan Ke Loc, Nguyen Giang Son, Nguyen Thi Phuong Trang, Le Thi Mai Linh, Le Thanh Son, Phylogenetic relationships of the Panax samples collected in Lai Chau province based on matK and ITS-rDNA sequences, Journal of Biology 12(2) (2014) 327-337 (in Vietnamese).[17] Nguyen Thi Phuong Trang, Le Thanh Son, Nguyen Giang Son, Phan Ke Long, A new ginseng species Panax sp. (Araliaceae) in Vietnam, Journal of Pharmacology 10 (2011) 59-63. [18] Pham Thi Ngoc, Pham Thanh Huyen, Nguyen Quynh Nga, Nguyen Minh Khoi, Morphological characteristics of genus Panax L. (Araliaceae) in Vietnam, Journal of Medicinal Materials, 22(3) (2017) 315-322.      


2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Teresa Santos ◽  
Carlos Fonseca ◽  
Tânia Barros ◽  
Raquel Godinho ◽  
Cristiane Bastos-Silveira ◽  
...  

Author(s):  
P.D.N. HEBERT ◽  
◽  
T.W.A. BRAUKMANN ◽  
S.W.J. PROSSER ◽  
S. RATNASINGHAM ◽  
...  

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