scholarly journals Identification of Antioxidant Compounds Structure Large-Leafed Mangrove Fruit

2016 ◽  
Vol 19 (2) ◽  
pp. 94
Author(s):  
Sabri Sudirman ◽  
Nurjanah Nurjanah ◽  
Agoes Mardiono Jacoeb
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Structure Prediction ◽  
Flavonoid Compound ◽  
Antioxidant Compounds ◽  
Ryukyu Islands ◽  
Bruguiera Gymnorrhiza ◽  
Compound Structure ◽  
The Pacific ◽  
The One

Antioxidants are compounds that can inhibit or prevent the oxidation of the easily oxidized substrate.<br />One of the plants as a potential source of bioactive compounds and antioxidant activity is large-leafed<br />mangrove (Bruguiera gymnorrhiza). This plant is commonly found in the Pacific region of Southeast Asia,<br />Ryukyu Islands, Micronesia and Polynesia (Samoa) to subtropical regions of Australia and has been used<br />by the society. This study aimed to determine the bioactive compounds structure of large-leafed mangrove<br />has the highest antioxidant activity. The compound structure prediction was done by Nuclear Magnetic<br />Resonance (NMR). The compound structure in the selected antioxidant fractions are flavonol, glikosilfalvon<br />and flavon. Those three compounds are flavonoid compound which has a great role as the one that has<br />antioxidant activity in large-leafed mangrove fruit.

scholarly journals Identification of Antioxidant Compounds Structure Large-Leafed Mangrove (Bruguiera gymnorrhiza) Fruit

2016 ◽  
Vol 19 (2) ◽  
pp. 94
Author(s):  
Sabri Sudirman ◽  
Nurjanah Nurjanah ◽  
Agoes Mardiono Jacoeb
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Structure Prediction ◽  
Flavonoid Compound ◽  
Antioxidant Compounds ◽  
Ryukyu Islands ◽  
Bruguiera Gymnorrhiza ◽  
Compound Structure ◽  
The Pacific ◽  
The One

Antioxidants are compounds that can inhibit or prevent the oxidation of the easily oxidized substrate.<br />One of the plants as a potential source of bioactive compounds and antioxidant activity is large-leafed<br />mangrove (Bruguiera gymnorrhiza). This plant is commonly found in the Pacific region of Southeast Asia,<br />Ryukyu Islands, Micronesia and Polynesia (Samoa) to subtropical regions of Australia and has been used<br />by the society. This study aimed to determine the bioactive compounds structure of large-leafed mangrove<br />has the highest antioxidant activity. The compound structure prediction was done by Nuclear Magnetic<br />Resonance (NMR). The compound structure in the selected antioxidant fractions are flavonol, glikosilfalvon<br />and flavon. Those three compounds are flavonoid compound which has a great role as the one that has<br />antioxidant activity in large-leafed mangrove fruit.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK KARANG LUNAK Klyxum Sp YANG DIKOLEKSI DARI DESA TUMBAK KECAMATAN POSUMAEN MINAHASA TENGGARA

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 205
Author(s):  
Elur Lonteng ◽  
Adithya Yudistira ◽  
Defny S Wewengkang
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Soft Coral ◽  
Health Sector ◽  
Antioxidant Compounds ◽  
Steroid Glycosides ◽  
Concentration Test ◽  
Ethanol Extracts

ABSTRACT Soft Coral Klyxum sp.. is a rich sources in bioactive compounds such as terpenoids, steroids, and steroid glycosides which are commonly used in the health sector. The purpose of this study was to determine the activity of antioxidant compounds from ethanol extracts of Klyxum sp. Klyxum sp., was extracted by maceration using ethanol as a solvent. As a parameter, testing of antioxidant activity was carried out using the DPPH (1,1-diphenil-2-picrylhydarzyl) method, which was measured using UV-Vis spectrophotometry at a wavelength of 517 nm. The results showed that the ethanol extracts of soft coral  Klyxum sp., proven to have antioxidant activity in each concentration test. Keywords: Klyxum sp. Soft Coral, Antioxidants, Extraction, DPPH   ABSTRAK Karang Lunak Klyxum sp. merupakan sumber yang kaya akan senyawa bioaktif seperti terpenoid, steroid, dan steroid glikosida yang biasa dimanfaatkan dalam dibidang kesehatan. Tujuan dari penelitian ini yaitu, untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol Karang Lunak Klyxum sp. Karang Lunak Klyxum sp. diekstraksi menggunakan metode maserasi dengan etanol sebagai pelarut. Sebagai parameter, pengujian aktivitas antioksidan dilakukan dengan metode DPPH (1,1-diphenil-2-picrylhydarzyl) yang diukur menggunakan Spektrofotometri UV-Vis pada panjang gelombang 517 nm. Hasil penelitian menunjukkan ekstrak etanol Karang Lunak Klyxum sp. terbukti memiliki aktivitas antioksidan disetiap konsentrasi pengujian. Kata Kunci : Karang Lunak Klyxum sp., Antioksidan, Ekstraksi, DPPH

scholarly journals Optimization The Effect of Roasting Conditions by Central Composite Design (CCD) Method on The Antioxidant Compounds of Opuntia Ficus Indica Seeds From Morocco

2020 ◽  
Author(s):  
Chakir El Guezzane ◽  
Hamza El Moudden ◽  
Hicham Harhar ◽  
Abdelkader Zarrouk ◽  
Mohamed Tabyaoui
Keyword(s):  
Antioxidant Activity ◽  
Central Composite Design ◽  
Principal Component ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Opuntia Ficus Indica ◽  
Optimal Value ◽  
Total Flavonoids Content ◽  
The One ◽  
Central Composite

Abstract The effect of roasting conditions on antioxidant activity Opuntia Ficus Indica seeds from Morocco and their phenolic compounds were optimized by the Central Composite Design (CDD) method. The CCD was used to optimize the roasting conditions: temperature (X1: 60–200 °C) and roasting time (X2: 10–50 min). The best roasting conditions were used in order to optimize the optimal value of the response : TPC (Total Phenolic Content): X1: 200°C, X2: 50min with 104.86±1.94GAE/g extract predicted response, TFC (Total Flavonoids Content) : X1: 200 °C, X2: 50 min with 81.23±0.90 mg QE/g extract predicted responses, TTC (Total Condensed Tannins Content): X1: 128.9°C, X2: 34.92min with 6.12±0.95 mg QAE/g extract) predicted response. Moreover, the optimal potential antioxidant activity by ABTS assay and DPPH assay was found in extracts of Opuntia Ficus Indica seeds roasted: at a high temperature of the roasting. Furthermore, the positive significant correlations were determined by the principal component analysis (PCA) on the one hand, between the antioxidant capacity of the extracts and their antioxidants compounds (TPC and TFC), and on the other hand, between the two assays ABTS and DPPH. Consequently, the results of this work indicated that roasted Opuntia Ficus Indica seeds from Morocco can be considered as an essential ingredient for human foods.

scholarly journals Antioxidant activity of shelf stable herbal seasoning incorporated with Pleurotus sajor-caju (oyster mushroom) powder

Food Research ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 57-64
Author(s):  
Saiful Bahri S. ◽  
Wan Rosli W.I.
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Milk Powder ◽  
Oyster Mushroom ◽  
Control Treatment ◽  
Antioxidant Compounds ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Storage Study ◽  
Pleurotus Sajor Caju

Pleurotus sajor-caju (oyster mushroom) has been consumed by people worldwide and they are known to produce different kinds of bioactive compounds. In this study, the methanolic extracts of herbal seasoning (HS) incorporated with Pleurotus sajor-caju (PSC) powder were evaluated for their antioxidant activity. A total of six different formulations with different levels of PSC powder at 0% (A), 20% (B), 40% (C), 60% (D), 80% (E) and 100% (F) to substitute coconut milk powder (CMP) were used in this study. The products were evaluated at the 0-month and 6-month of storage. HS (F) recorded the highest TPC (total polyphenol content) (1823.84±0.84 mg GAE/g), ABTS (2,2-azino-bis3-ethylbenzothiazoline-6-sulphonic acid) (71%) and FRAP (Ferric Reducing Antioxidant Power) assays (60.52 mg AAE/g) at 0 month of the storage study. At the 6-month of storage study, the highest TPC, ABTS and FRAP assay were recorded in HS(F) (1539.23±0.61 mg GAE/g), (59%) and (27.51 mg AAE/g), respectively. HS(A), the control treatment recorded the lowest TPC, ABTS and FRAP assay at the 6-month of the storage study. The addition of PSC powder to replace CMP may influence the presence of bioactive compounds in HS. The reduction of antioxidant after 6 months of storage might be due to the instability of other antioxidant compounds such as carotenoids and phenols. In conclusion, after the 6-month of the storage study, this edible mushrooms still exert some antioxidative capacities thus potentially being expended as a natural antioxidant in thermally processed food products.

scholarly journals Effect of Rootstock and Harvesting Period on the Bioactive Compounds and Antioxidant Activity of Two Orange Cultivars (‘Salustiana’ and ‘Sanguinelli’) Widely Used in Juice Industry

Processes ◽  
2020 ◽  
Vol 8 (10) ◽  
pp. 1212
Author(s):  
José L. Ordóñez-Díaz ◽  
Aurea Hervalejo ◽  
Gema Pereira-Caro ◽  
José M. Muñoz-Redondo ◽  
Estefanía Romero-Rodríguez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Organic Acid ◽  
Bioactive Compounds ◽  
Antioxidant Compounds ◽  
Total Carotenoids ◽  
Hydrophilic Fraction ◽  
Citrus Volkameriana ◽  
Hydrophobic Fraction ◽  
Ascorbic Acids ◽  
The Impact

Oranges are a rich source of bioactive compounds with recognized benefits for human health. To guarantee high quality and production levels, citrus farms usually employ the combination of selected cultivars with well adapted rootstocks. This study analyzes the impact of four different citrus rootstocks (Forner-Alcaide no.5, ‘Cleopatra mandarin’, Citrus volkameriana and Carrizo citrange) on the bioactive compounds and antioxidant activity of two orange cultivars (‘Salustiana’ and ‘Sanguinelli’) widely used in the orange juice industry. For the hydrophilic fraction, the phenolic compound, anthocyanin, and organic acid profiles were determined by HPLC-DAD-HRMS, and the antioxidant activity by ABTS, DPPH, and ORAC assays. Besides, the total carotenoids and ABTS concentrations were calculated for the hydrophobic fraction. A set of three flavanones, one flavone, and eight anthocyanins were tentatively identified and quantified in the orange cultivars tested. The predominant phenolic compounds obtained in both orange cultivars were hesperidin and narirutin, while cyanidin-3-O-(6″-malonyl) glucoside followed by cyanidin-3-O-rutinoside and cyanidin-3-O-glucoside were the main anthocyanins found in the ‘Sanguinelli’ cultivar. Citric acid, followed by malic, oxalic, and ascorbic acids were the main organic acids. The higher amount of antioxidant compounds was found in fruit from the Forner-Alcaide no.5 rootstock. These results indicate that Forner-Alcaide n.5 affects positively the phenolic and organic acid composition and the antioxidant capacity of ‘Sanguinelli’ and ‘Salustiana’ cultivars, and is therefore a good option for the sector based on the healthy promoting properties.

scholarly journals Multiple Emulsions with Extracts of Cactus Pear Added in A Yogurt: Antioxidant Activity, In Vitro Simulated Digestion and Shelf Life

Foods ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 429 ◽  
Author(s):  
Antonio de Jesús Cenobio-Galindo ◽  
Gilberto Díaz-Monroy ◽  
Gabriela Medina-Pérez ◽  
M. Jesús Franco-Fernández ◽  
Fanny Emma Ludeña-Urquizo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Shelf Life ◽  
Bioactive Compounds ◽  
Gum Arabic ◽  
In Vitro Digestion ◽  
Antioxidant Compounds ◽  
Multiple Emulsion ◽  
Cactus Pear ◽  
Simulated Digestion

Consumers demand so-called natural in which additive and antioxidant preservatives are from natural origin. Research focuses in using extracts from plants and fruits that are rich in bioactive compounds such as phenolics and betalains, but these are also prone to interact with proteins and are exposed to suffer degradation during storage. In this work, we developed a fortified yogurt with the addition of betalains and polyphenols from cactus pear extract encapsulated in a multiple emulsion (ME) (W1/O/W2). Different formulations of ME were made with two polymers, gum arabic (GA) and maltodextrin (MD) and with the best formulation of ME four types of yogurt were prepared using different % (w/w) of ME (0%, 10%, 20% and 30%). Bioactive compounds, antioxidant activity, color and lactic acid bacteria (LAB) were analyzed in the different yogurts over 36 days of shelf life. Furthermore, in vitro simulated digestion was evaluated. The yogurts had significant (p < 0.05) differences and the ME protected the bioactive compounds, activity of antioxidants and color. The ME did not affect the viability of LAB during 36 days of storage. The in vitro digestion showed the best bioaccessibilities of antioxidant compounds with the yogurts with ME.

scholarly journals ANTIOXIDANT ACTIVITY of THE SECONDARY METABOLITES PRODUCED by ENDOPHYTIC FUNGI ISOLATED from JERUJU (Acanthus ilicifolius L.) PLANT

2019 ◽  
Vol 5 (2) ◽  
Author(s):  
Amanda Rahmaniah Putri ◽  
Salni Salni ◽  
Harry Widjajanti
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Flavonoid Compound ◽  
Antioxidant Compounds ◽  
Strong Activity ◽  
Antioxidant Compound ◽  
Diaporthe Phaseolorum ◽  
Ic50 Value ◽  
Dpph Method

Twelve endophytic fungi were isolated from Jeruju plant. Three of which have the best antioxidant activity, namely Culvularia lunata DJS1, Diaporthe phaseolorum DJS2, and Colletotrichum siamense BJS4. The fungi can produce secondary metabolites that have the potential as antioxidant compounds. This study aims to isolate the antioxidant compound using a chromatography column and test the antioxidant activity using DPPH method. Isolation of secondary metabolites produced 40 eluates. The IC50 value of DJS111 and BJS42 eluates were 10.20 µg/ml and 20.89 µg/ml, which is an antioxidant compound with very strong activity. The IC50 value of DJS24 eluate was 54.45 µg/ml, which is strong antioxidant activity. DJS111 eluate has antioxidant activity that approaches to ascorbic acid as a standart antioxidant compound (7.76µg/ml). From TLC, DJS111 contains flavonoid compound and DJS24 and BJS42 contain terpenoid compound.

scholarly journals Bioactive compounds and in vitro antioxidant activity of pulps from fruits from the Brazilian atlantic forest

2019 ◽  
Vol 42 ◽  
pp. e44503 ◽  
Author(s):  
Ana Carolina Leme Castelucci ◽  
Paula Porrelli Moreira da Silva ◽  
Marta Helena Fillet Spoto
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Multivariate Statistical ◽  
The Family ◽  
The One ◽  
Free Radical Activity

The fruits belonging to the family Myrtaceae are known sources of compounds with functional characteristics. Nevertheless, the studies are focused only on some species of this family. In this sense, we aimed to quantify the bioactive compounds present in the pulps of cambuci, feijoa, uvaia and grumixama; to evaluate the in vitro antioxidant capacity of each one and to correlate the contribution of these bioactive compounds with the antioxidant activity of each fruit pulp. For this, the compounds ascorbic acid, phenolic compounds, carotenoids, and flavonoids were quantified for the pulps of cambuci, feijoa, uvaia, and grumixama, as well as the in vitro antioxidant capacity by the methods DPPH and ABTS. The results were evaluated by multivariate statistical techniques. The pulps present good antioxidant potential, the one from cambuci presented the highest values for antioxidant activity given by the method DPPH (61.86 µg of Trolox g-1), that from uvaia was prominent by the presence of ascorbic acid (85.40 ascorbic acid 100 g-1) and that from feijoa, by the flavonoid contents (62.45 mg quercetin g-1) and phenolic compounds (10.21 mg gallic acid equivalent g–1). A correlation was observed between pulp antioxidant capacity and the contents of ascorbic acid and carotenoids; on the other hand, the phenolic compounds and flavonoids little contributed for the anti-free radical activity of the methods DPPH and ABTS.

Temulawak (Curcuma  xanthorrhizaRoxb.) belongs to the family Zingiberaceae, has been empirically used as herbal medicines. The research was aimed to evaluate three promising lines of Temulawak based on their high bioactive contents (xanthorrhizol and curcuminoid) and its in vitro bioactivity (antioxidant and toxicity), and to obtain information on agrobiophysic environmental condition which produced high bioactive compounds. The xanthorrhizol and curcuminoid contents were measured by HPLC. In vitro antioxidant and toxicity were determined by DPPH (1,1-diphenyl-2-picryl-hydrazyl) method and BSLT (Brine Shrimp Lethality Test). The result showed that promising line A produced the highest yield of bioactive and bioactivity, i.e. 0.157 and 0.056 g plant-1of xanthorrizol and curcuminoid respectively. The IC50 of antioxidant activity was 65.09 mg L-1and LC50of toxicity was 69.05 mg L-1. In this study, Cipenjo had the best temulawak performance than two other locations. According to the agrobiophysic parameters, Cipenjo environmental condition was suitable for temulawak cultivation with temperature 28-34 ºC, rainfall ± 223.97 mm year-1 and sandy clay soil. Keywords: antioxidant, curcuminoid, promising lines, temulawak, xanthorrhizol

1970 ◽  
Vol 40 (2) ◽  
Author(s):  
Waras Nurcholis ◽  
Edy Djauhari Purwakusumah ◽  
Mono Rahardjo ◽  
Latifah K. Darusman
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Brine Shrimp ◽  
Clay Soil ◽  
Herbal Medicines ◽  
Environmental Condition ◽  
In Vitro Bioactivity ◽  
The Family ◽  
Promising Line

Temulawak (Curcuma  xanthorrhizaRoxb.) belongs to the family Zingiberaceae, has been empirically used as herbal medicines. The research was aimed to evaluate three promising lines of Temulawak based on their high bioactive contents (xanthorrhizol and curcuminoid) and its in vitro bioactivity (antioxidant and toxicity), and to obtain information on agrobiophysic environmental condition which produced high bioactive compounds. The xanthorrhizol and curcuminoid contents were measured by HPLC. In vitro antioxidant and toxicity were determined by DPPH (1,1-diphenyl-2-picryl-hydrazyl) method and BSLT (Brine Shrimp Lethality Test). The result showed that promising line A produced the highest yield of bioactive and bioactivity, i.e. 0.157 and 0.056 g plant-1of xanthorrizol and curcuminoid respectively. The IC50 of antioxidant activity was 65.09 mg L-1and LC50of toxicity was 69.05 mg L-1. In this study, Cipenjo had the best temulawak performance than two other locations. According to the agrobiophysic parameters, Cipenjo environmental condition was suitable for temulawak cultivation with temperature 28-34 ºC, rainfall ± 223.97 mm year-1 and sandy clay soil. Keywords: antioxidant, curcuminoid, promising lines, temulawak, xanthorrhizol

Immobilization of Oat Bran Polyphenols in Complex Coacervates of Whey Protein and Malthodextrin

2020 ◽  
Vol 50 (3) ◽  
pp. 460-469
Author(s):  
Damir Zyaitdinov ◽  
Alexandr Ewteew ◽  
Anna Bannikova
Keyword(s):  
Antioxidant Activity ◽  
Enzymatic Hydrolysis ◽  
Whey Protein ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Wall Material ◽  
Total Phenolic ◽  
Oat Bran ◽  
Kinetics Of

Introduction. Bioactive compounds are a very popular topic of modern food science, especially when it concerns obtaining polyphenols from cereals. The antiradical, antioxidant, and anti-inflammatory properties of these ingredients allow them to inhibit and prevent coronary, artery, and cardiovascular diseases, as well as several types of cancer. Encapsulation is an effective technology that protects bioactive ingredients during processing and storage. In addition, it also prevents any possible interaction with other food constituents. The research objective was to obtain effective tools of controlled delivery of bioactive compounds. The study featured whey protein as a wall material in combination with maltodextrin to encapsulate the bioactives from oat bran. Study objects and methods. The processed material was oat bran. The technology of its biotransformation was based on ultrasound processing and enzymatic hydrolysis. The antioxidant properties were determined using a coulometer of Expert – 006-antioxidants type (Econix-Expert LLC, Moscow, Russia). Separation and quantitative determination of extract were followed using a Stayer HPLC device (Akvilon, Russia) and a system column Phenomenex Luna 5u C18(2) (250×4.6 mm). The total phenolic content was measured by a modified Folin-Ciocalteu method. To prepare microcapsules, whey protein concentrate (WPC) and maltodextrin (MD) solutions were mixed at ratios 6:4, 4:6, and 5:5. After that, the mixes were treated by ultrasonication and 10% w/w of guar gum solution as double wall material. The encapsulation efficiency (EE) was determined as a ratio of encapsulated phenolic content to total phenolic content. A digestion protocol that simulates conditions of the human gastric and intestinal tract was adapted to investigate the release kinetics of the extracts. Results and discussion. Ferulic acid is the main antioxidant in cereals. Its amount during extraction was consistent with published data: 9.2 mg/mL after ultrasound exposure, 9.0 mg/mL after enzymatic extraction, and 8.6 mg/mL after chemical treatment. The antioxidant activity of the obtained polyphenols was quite high and reached 921 cu/mL. It depended on the concentration of the preparation in the solution and the extraction method. The polyphenols obtained by ultrasonic exposure and enzyme preparations proved to have a more pronounced antioxidant activity. The highest EE (95.28%) was recorded at WPC:MD ratio of 60:40. In vitro enzymatic hydrolysis protocol simulating digestion in the gastrointestinal tract was used to study the effect of capsule structural characteristics on the kinetics of polyphenol release. The percentage of o polyphenols released from capsules ranged from 70% to 83% after two hours of digestion, which confirmed the effectiveness of microencapsulation technology. Conclusion. The research confirmed the possibility of using polyphenols obtained by the biotechnological method from oat bran as functional ingredients. Eventually, they may be used in new functional products with bifidogenic properties. Whey protein can be used to encapsulate polyphenols as the wall material of microcapsules.

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