scholarly journals Search of markers of alcoholization among ghrelin derivatives

2013 ◽  
Vol 11 (2) ◽  
pp. 36-40
Author(s):  
Platon Platonovich Khokhlov ◽  
Yevgeniy Rudolfovich Bychkov ◽  
Roman Olegovich Roik ◽  
Andrey Andreyevich Lebedev ◽  
Petr Dmitriyevich Shabanov
Keyword(s):  
Quantitative Determination ◽  
Enzyme Immunoassay ◽  
Alcohol Withdrawal ◽  
Biochemical Marker ◽  
Ethanol Withdrawal ◽  
Nonparametric Statistic ◽  
Unacylated Ghrelin ◽  
Chronic Alcoholization

The quantitative determination of unacylated ghrelin in rat sera in the course of prolonged (chronic) alcoholization during 6 months followed by alcohol withdrawal (in 1 and 7 days) was made by means of the rat unacylated ghrelin enzyme immunoassay kit (SPIBIO, France). Unacylated ghrelin was revealed in all samples both in control and experimental groups. The row data were treated by means of parametric and nonparametric statistic tests. The level of unacylated ghrelin was shown to double reduce in the course of prolonged alcoholization in rats. Ethanol withdrawal recovered the polypeptide content gradually with maximal indexes in 7 days after alcohol abolition but lower than in control rats. The possibilities of the use of unacylated ghrelin as a biochemical marker of alcoholization and its consequences are discussed.

Quantitative determination of immunoglobulins in the cerebrospinal fluid based on their light chain properties using a solid-phase enzyme immunoassay

1984 ◽  
Vol 142 (1) ◽  
pp. 11-20 ◽  
Author(s):  
Hirohata Shunsei ◽  
Inoue Tetsufumi ◽  
Yamada Akio ◽  
Hirose Shunichi ◽  
Miyamoto Terumasa
Keyword(s):  
Cerebrospinal Fluid ◽  
Quantitative Determination ◽  
Enzyme Immunoassay ◽  
Light Chain ◽  
Solid Phase

scholarly journals Quantitative Determination of Digoxin in Human Serum by Enzyme Immunoassay

1980 ◽  
Vol 6 (3) ◽  
pp. 196-198
Author(s):  
AKIO AJIMURA ◽  
MASAYUKI TOTANI ◽  
SHUJI SUGAYAMA
Keyword(s):  
Quantitative Determination ◽  
Human Serum ◽  
Enzyme Immunoassay

Comparison of Results for Quantitative Determination of Morphine by Radioimmunoassay, Enzyme Immunoassay, and Spectrofluorometry

1975 ◽  
Vol 20 (4) ◽  
pp. 10316J ◽  
Author(s):  
V. R. Spiehler ◽  
Dwight Reed ◽  
R. H. Cravey ◽  
W. P. Wilcox ◽  
R. F. Shaw ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Enzyme Immunoassay ◽  
Comparison Of Results

scholarly journals A New Enzyme Immunoassay for the Quantitative Determination of Classical Autotaxins (ATXα, ATXβ, and ATXγ) and Novel Autotaxins (ATXδ and ATXε)

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0130074 ◽  
Author(s):  
Yasunori Tokuhara ◽  
Makoto Kurano ◽  
Satoshi Shimamoto ◽  
Koji Igarashi ◽  
Takahiro Nojiri ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Enzyme Immunoassay

scholarly journals ENZYME IMMUNOASSAY KIT FOR THE DETERMINATION OF DEOXYNIVALENOL

2018 ◽  
Vol 54 (3) ◽  
pp. 319-328 ◽  
Author(s):  
I. I. Vashkevich ◽  
O. S. Kuprienko ◽  
I. V. Gorbachova ◽  
A. A. Yastrebova ◽  
T. V. Terentieva ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Horseradish Peroxidase ◽  
Quantitative Determination ◽  
Enzyme Immunoassay ◽  
Bovine Serum ◽  
Polyclonal Antibodies ◽  
Agricultural Products ◽  
Accuracy And Precision ◽  
Modern Level

A reagent kit EIA-DEOXYNIVALENOL for the determination of mycotoxin deoxynivalenol (DON) in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The basic components of the kit are polyclonal antibodies to DON, obtained as a result of immunization of rabbits with a conjugate of DON with bovine serum albumin and a conjugate of horseradish peroxidase with DON. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of DON content of agricultural products in a range of 0.2 to 6.0 mg/kg with proper accuracy and precision. The limit of quantitative determination of DON in grain and cereal foods does not exceed 0.2 mg/kg.

Enzyme Immunoassay for the Quantitative Determination of Galanthamine

Planta Medica ◽  
1993 ◽  
Vol 59 (05) ◽  
pp. 442-446 ◽  
Author(s):  
Alexander Poulev ◽  
Brigitte Deus-Neumann ◽  
Meinhart Zenk
Keyword(s):  
Quantitative Determination ◽  
Enzyme Immunoassay
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