Comparison of Results for Quantitative Determination of Morphine by Radioimmunoassay, Enzyme Immunoassay, and Spectrofluorometry

1975 ◽  
Vol 20 (4) ◽  
pp. 10316J ◽  
Author(s):  
V. R. Spiehler ◽  
Dwight Reed ◽  
R. H. Cravey ◽  
W. P. Wilcox ◽  
R. F. Shaw ◽  
...  
1984 ◽  
Vol 142 (1) ◽  
pp. 11-20 ◽  
Author(s):  
Hirohata Shunsei ◽  
Inoue Tetsufumi ◽  
Yamada Akio ◽  
Hirose Shunichi ◽  
Miyamoto Terumasa

1980 ◽  
Vol 6 (3) ◽  
pp. 196-198
Author(s):  
AKIO AJIMURA ◽  
MASAYUKI TOTANI ◽  
SHUJI SUGAYAMA

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0130074 ◽  
Author(s):  
Yasunori Tokuhara ◽  
Makoto Kurano ◽  
Satoshi Shimamoto ◽  
Koji Igarashi ◽  
Takahiro Nojiri ◽  
...  

Author(s):  
I. I. Vashkevich ◽  
O. S. Kuprienko ◽  
I. V. Gorbachova ◽  
A. A. Yastrebova ◽  
T. V. Terentieva ◽  
...  

A reagent kit EIA-DEOXYNIVALENOL for the determination of mycotoxin deoxynivalenol (DON) in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The basic components of the kit are polyclonal antibodies to DON, obtained as a result of immunization of rabbits with a conjugate of DON with bovine serum albumin and a conjugate of horseradish peroxidase with DON. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of DON content of agricultural products in a range of 0.2 to 6.0 mg/kg with proper accuracy and precision. The limit of quantitative determination of DON in grain and cereal foods does not exceed 0.2 mg/kg.


Planta Medica ◽  
1993 ◽  
Vol 59 (05) ◽  
pp. 442-446 ◽  
Author(s):  
Alexander Poulev ◽  
Brigitte Deus-Neumann ◽  
Meinhart Zenk

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Chong-Un Cheong ◽  
Ching-Ping Chang ◽  
Chien-Ming Chao ◽  
Bor-Chih Cheng ◽  
Chung-Zhing Yang ◽  
...  

It remains unclear whether etanercept penetrates directly into the contused brain and improves the outcomes of TBI by attenuating brain contents of TNF-αand/or stimulating newly formed neurogenesis. Rats that sustained TBI are immediately treated with etanercept. Acute neurological and motor injury is assessed in all rats the day prior to and 7 days after surgery. The numbers of the colocalizations of 5-bromodeoxyuridine and doublecortin specific markers in the contused brain injury that occurred during TBI were counted by immunofluorescence staining. Enzyme immunoassay for quantitative determination of TNF-αor etanercept in brain tissues is also performed. Seven days after systemic administration of etanercept, levels of etanercept can be detected in the contused brain tissues. In addition, neurological and motor deficits, cerebral contusion, and increased brain TNF-αcontents caused by TBI can be attenuated by etanercept therapy. Furthermore, the increased numbers of the colocalizations of 5-bromodeoxyuridine and doublecortin specific markers in the contused brain tissues caused by TBI can be potentiated by etanercept therapy. These findings indicate that systemically administered etanercept may penetrate directly into the contused brain tissues and may improve outcomes of TBI by reducing brain contents of TNF-αand by stimulating newly formed neurogenesis.


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