scholarly journals Characteristic features of type B Haemophilus Influenzae-caused purulent meningitis in children

2018 ◽  
Vol 35 (5) ◽  
pp. 32-35
Author(s):  
L. Yu. Grishkina ◽  
I. I. Lvova ◽  
I. N. Trefilov ◽  
N. V. Banko ◽  
O. V. Odintsova
Keyword(s):  
Latex Agglutination ◽  
Acute Onset ◽  
Type B ◽  
Haemophilus Influenza ◽  
Chain Reaction ◽  
Purulent Meningitis ◽  
Polymerase Chain ◽  
Characteristic Features ◽  
Meningitis In Children ◽  
Disseminated Intravascular Clotting

Aim. To study the clinical peculiar features of type b Haemophilus influenza-caused purulent meningites among children in Perm. Materials and methods. Twenty children with purulent meningitis were examined. Clinical and bacteriological studies, latex-agglutination reaction, polymerase chain reaction (PCR) were used. Results. Most patients with Hib-meningitis (70 %) were hospitalized in grave condition during the first three days of disease with fever, meningeal symptoms. Spastic syndrome was diagnosed in 5 patients, disseminated intravascular clotting syndrome – in 6 patients. Conclusions. Hib-meningitis in 80 % of cases is developing in children aged under two, has an acute onset and severe course with general cerebral and meningeal symptoms, changes in blood and liquor, typical for bacterial infection, high neutrophilic pleocytosis and hyperproteinorrhea; it is frequently accompanied by complications. In etiological diagnosis of Hib-meningitis PCR is the most informative method.

scholarly journals Incidence of bacterial meningitis in Asia using enhanced CSF testing: polymerase chain reaction, latex agglutination and culture

2007 ◽  
Vol 135 (7) ◽  
pp. 1217-1226 ◽  
Author(s):  
W. A. KENNEDY ◽  
S-J. CHANG ◽  
K. PURDY ◽  
T. LE ◽  
P. E. KILGORE ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Bacterial Culture ◽  
East Asian ◽  
Latex Agglutination ◽  
Type B ◽  
Chain Reaction ◽  
Surveillance Studies ◽  
Polymerase Chain

SUMMARYTo enhance the detection of bacterial meningitis in an East Asian surveillance study, we employed cerebrospinal fluid (CSF) bacterial culture, latex agglutination (LA) and polymerase chain reaction–enzyme immunoassay (PCR–EIA) testing forHaemophilus influenzaetype b (Hib) andStreptococcus pneumoniae(Sp). The sensitivity and specificity of CSF PCR–EIA testing was compared to LA and culture. A meningitis case was defined by one positive result for any of the three tests. The sensitivity ofH. influenzaeCSF PCR–EIA, LA, and culture was 100%, 40% and 57·5% respectively; and for Sp CSF PCR–EIA, LA and culture, the sensitivity was 100%, 58·3% and 66·7%, respectively. Hib and Sp specificity was 100% by each method. CSF PCR–EIA was more sensitive than culture or LA for the detection of Hib and Sp meningitis cases increasing their incidence by 74% and 50% compared to culture respectively. CSF PCR–EIA should be included for the detection of bacterial meningitis in surveillance studies.

scholarly journals Molecular identification by multiplex polymerase chain reaction of Pasteurella multocida in cattle and buffaloes in Baghdad

2014 ◽  
Vol 38 (1) ◽  
pp. 99-106
Author(s):  
Ihab G. M. AL-Shemmari
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Pcr ◽  
Multiplex Polymerase Chain Reaction ◽  
Pasteurella Multocida ◽  
Type B ◽  
Chain Reaction ◽  
Blood Samples ◽  
Nasal Swabs ◽  
Polymerase Chain ◽  
B 31

The aim of this study was to identify pasteurella multocida and their types by PCR in cattle’s and buffaloesi bagdad from March to August 2012 on 204 animals , including 102 cattle and 102 buffaloes at slaughter houses from Baghdad .Blood samples and nasal swaps were collected , before slaughtering and lung tissues of slaughtered animal , and from 54 clinically suspected cases of pasteurellosis , including 27 bovines ,and 27 buffaloes the samples taken included blood and nasal swabs . Pasteurellamultocida were isolated from 94 animals include 49 cattle 45 buffaloes. The typing of the isolates by multiplex PCR for genotyping Pasteuerllamultocida revealed 93 isolates of type B , 31 from cattle and 62 from buffaloes ,and 81 isolates of type A , 55 from cattle and 26 from buffaloes .

scholarly journals Precision of genotyping of Epstein-Barr virus by polymerase chain reaction using three gene loci (EBNA-2, EBNA-3C, and EBER): predominance of type A virus associated with Hodgkin's disease [published erratum appears in Blood 1993 Oct 1;82(7):2268]

Blood ◽  
1993 ◽  
Vol 81 (12) ◽  
pp. 3372-3381 ◽  
Author(s):  
JC Lin ◽  
SC Lin ◽  
BK De ◽  
WC Chan ◽  
BL Evatt ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cell Lines ◽  
Epstein Barr Virus ◽  
Point Mutations ◽  
Type A ◽  
Type B ◽  
Chain Reaction ◽  
Barr Virus ◽  
Polymerase Chain ◽  
Epstein Barr

Abstract To precisely determine the genotype of Epstein-Barr virus (EBV) in Hodgkin's disease (HD), we simultaneously analyzed three divergent gene loci (EBNA-2, EBNA-3C, and EBER) that distinguish type A and B viruses. The primers designed to amplify these three gene loci encompass either type-specific deletion sequences (EBNA-2 and EBNA-3C) or type-specific point mutations (EBER) that identify the virus strain based on the sizes of the polymerase chain reaction (PCR)-amplified products or the mobility shifts in single-strand conformation polymorphism analysis. The locations of point mutations were identified by direct sequencing of the PCR-amplified DNA. We analyzed 15 EBV-infected cell lines and found a good correlation between EBNA-2 and EBNA-3C typing results. In contrast, approximately 33% of the cell lines analyzed maintained type A sequences in EBNA-2 and EBNA-3C genes while carrying type B sequences in the EBER region. Data obtained from analysis of cell lines served as a reference for studying HD samples. EBV DNA was detected in about 70% of HD. Among the EBV-positive samples, 56% were associated with type A virus, 13% with type B, and 31% with dual viral sequences. Thus, type A virus is predominant in HD. Based on the histology, the frequencies of EBV positivity were 83%, 71%, and 33% for mixed cellularity, nodular sclerosis, and lymphocyte predominance, respectively. The detection of high frequency of both type A and B sequences in HD may provide a lead in investigating the role of dual viral infection in EBV pathogenesis.

Prevalence of the Enterotoxin Gene and Clonality of Clostridium perfringens Strains Associated with Food-Poisoning Outbreaks

1998 ◽  
Vol 61 (2) ◽  
pp. 240-243 ◽  
Author(s):  
J. RIDELL ◽  
J. BJÖRKROTH ◽  
H. EISGRŰBER ◽  
B. SCHALCH ◽  
A. STOLLE ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Food Poisoning ◽  
Latex Agglutination ◽  
Pulsed Field Gel Electrophoresis ◽  
Enterotoxin Gene ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Clostridium Perfringens Enterotoxin ◽  
Polymerase Chain

The prevalence of the enterotoxin gene in a well-characterized collection of 71 Clostridium perfringens strains from 36 separate food-poisoning cases or outbreaks was analyzed with the polymerase chain reaction (PCR). The clonality of 39 strains originating from 14 outbreaks where at least two isolates were available was studied with pulsed-field gel electrophoresis (PFGE) using SmaI and ApaI restriction endonucleases. The cpe gene PCR assay was found to correlate well with Clostridium perfringens enterotoxin (CPE) production in vitro with reverse passive latex agglutination. Of the C. perfringens food and clinical food-poisoning isolates 24 (86%) and 38 (88%) were cpe-positive, respectively. Different PFGE patterns indicated that multiple cpe-positive clones are frequently present within one outbreak. The existence of cpe-positive and negative isolates with identical or nearly identical PFGE patterns in a single outbreak suggests that the cpe gene may be in a movable genetic element.

scholarly journals Acute‐onset smell and taste disorders in the context of COVID‐19: a pilot multicentre polymerase chain reaction based case–control study

2020 ◽  
Vol 27 (9) ◽  
pp. 1738-1741 ◽  
Author(s):  
Á. Beltrán‐Corbellini ◽  
J. L. Chico‐García ◽  
J. Martínez‐Poles ◽  
F. Rodríguez‐Jorge ◽  
E. Natera‐Villalba ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Case Control Study ◽  
Case Control ◽  
Acute Onset ◽  
Chain Reaction ◽  
Taste Disorders ◽  
Polymerase Chain ◽  
Control Study

scholarly journals Apparent Outbreaks of Clostridium Difficile-Associated Diarrhea in Horses in a Veterinary Medical Teaching Hospital

1995 ◽  
Vol 7 (3) ◽  
pp. 343-346 ◽  
Author(s):  
Bruce R. Madewell ◽  
Yajarayma J. Tang ◽  
Spencer Jang ◽  
John E. Madigan ◽  
Dwight C. Hirsh ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Clostridium Difficile ◽  
Teaching Hospital ◽  
Polymerase Chain Reaction Amplification ◽  
Acute Onset ◽  
Selective Medium ◽  
Chain Reaction ◽  
Medical Teaching ◽  
Polymerase Chain ◽  
Toxigenic Strains

Intestinal colonization with toxigenic strains of Clostridium difficile was documented in 9 of 10 horses with acute onset diarrhea in a veterinary medical teaching hospital, whereas a similar isolate was detected in only 1 of 23 other horses without diarrhea in the hospital. One horse with diarrhea was infected simultaneously with both C. difficile and Salmonella krefeld. Clostridium difficile was detected by fecal culture on selective medium, confirmed with a latex particle agglutination test, and identified as toxigenic by polymerase chain reaction amplification of toxin A and toxin B gene sequences. Using an arbitrarily-primed polymerase chain reaction, 6 distinct C. difficile isolates were detected in the feces of the 9 affected horses at the time of the outbreak of diarrhea.

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