scholarly journals The state of the oxidation-reduction system in patients with primary open angle glaucoma

2012 ◽  
Vol 93 (3) ◽  
pp. 488-490
Author(s):  
I R Gazizova

Aim. To study the state of the oxidation-reduction system in patients with primary open-angle glaucoma. Methods. Conducted were biochemical studies of the state of the oxidation-reduction system in 30 patients with primary open-angle glaucoma. The level of lipid peroxidation of cell membranes was determined in blood serum by the method of accumulation of malondialdehyde based on its interaction with thiobarbituric acid. The state of the antioxidant system was assessed according to the activity of superoxide dismutase, catalase, glutathione reductase and the content of thiol disulfide groups in the blood. The concentration of lactate in the blood serum was determined using an automated analyzer. Results. In patients with a chronic course of glaucoma elevated levels of oxidized thiol groups of proteins, increased activity of antioxidant enzymes, activation of lipid peroxidation processes, increased lactate content have been identified. Conclusion. Changes in the oxidation-reduction system in patients with primary open-angle glaucoma may be a sign of mitochondrial dysfunction.

Author(s):  
F.J. Hernández-Martínez ◽  
P. Piñas-García ◽  
A.V. Lleó-Pérez ◽  
V.C. Zanón-Moreno ◽  
E. Bendala-Tufanisco ◽  
...  

Author(s):  
L. S. Pavlova ◽  
I. R. Gazizova ◽  
V. N. Alekseev ◽  
Yu. A. Alekseev

This review of modern literature presents the issue of early diagnosis of primary open-angle glaucoma (POAG) which is one of the most important problems in ophthalmology. According to the World Health Organization (WHO), the number of glaucoma patients all over the world ranges from 60.5 to 105 million people, and in the next 10 years it will increase by another 10 million. The subtle onset of the disease usually leads to late diagnosis, since visual field defects are often first detectable by visual field testing when 25–35% of retinal ganglion cells have already been lost. In modern studies, it has been established that the cell death in POAG is accompanied by oxidative stress. It is considered a key factor indicating the pathological process of a fairly large number of chronic diseases of the whole body, including complicated eye pathologies and inflammatory processes. The resulting free radicals oxidize lipids and phospholipids which leads to their peroxidation and triggers damage to cell membranes. The lipid peroxidation is one of obligatory processes occurring in neurodegenerative conditions including POAG. Modern technologies analyzing lipid peroxidation may accelerate the diagnosis, to identify glaucoma biomarkers at an earlier stage, and as a result to maintain quality of vision more efficiently. These studies were carried out in different countries and using different methods such as serological proteomic analysis, chromatography and mass spectrometry, and new technological approaches such as MultiOmics. The study of the protein profile, lipase activity, nitric oxide concentration, activity of carbonic anhydrase enzyme, malondialdehyde (MDA) and other compounds are also very informative. Various fluids were taken as a basis for the study: tears, aqueous humor of the anterior chamber, blood and its components. Together these technologies enable to evaluate the oxidative process in different ways. Earlier diagnosis of glaucoma is very important as it will enable to maintain the quality of vision for a longer time and reduce the rate of the disease progression. Keywords: primary open-angle glaucoma, lipid peroxidation, lacrimal fluid, serum.


2019 ◽  
Author(s):  
Valeria Yashina ◽  
Tatiana Sokolovskaya ◽  
Pavel Volodin

Laser interventions as microinvasive methods of treatment of patients with primary open-angle glaucoma are widely used in clinical practice because they are effective, safe, the period of rehabilitation after this type of treatment is short, the risk of postoperative complications is minimal. In the scientific literature there are data on the effect of laser radiation on the corneal endothelial cells, that is why this aspect should be investigated in the development and use of new laser technologies in the treatment of patients with primary open-angle glaucoma. One of the most modern methods of corneal examination is known to be lifetime confocal microscopy, which allows to visualize corneal tissue at the cellular level. Purpose: to study the effect of laser radiation on the state of corneal endothelial cells in YAG-laser activation of trabecula in patients with primary open-angle glaucoma. Materials and methods. Sixteen eyes of 15 patients were included in the analysis: 7 men and 8 women aged 53 to 74 years. The mean age of patients was 65.8±5.35 years. All patients underwent YAG-laser activation of trabecula: Nd-YAG laser, 1064nm, 0.8-1.2MJ, 30NS pulse duration, 10-15µm a spot diameter, 55-70 pulses in the circle. In different periods of observation (before laser treatment, 1 hour, 1 week, 1 month, 6 months after laser intervention) the level of IOP was monitored, the state of endothelial corneal cells was studied by confocal microscopy. Results. According to the results of confocal microscopy statistically significant difference between the average density of endothelial cells, polymegathism, and pleomorphism have not been identified. Conclusion. The study did not reveal a negative impact of YAG-laser activation of trabecula on the endothelium of the cornea, which indicates the safety of this technology.


Author(s):  
E. N. Simakova ◽  
O. V. Stenkova

Introduction. Glaucoma is one of the most significant eye diseases. It is often diagnosed, not always amenable to therapy, and can lead to a complete loss of visual functions. In recent years, the method of osteopathic correction has become widespread as one of the effective methods of treatment and rehabilitation of patients with pathologies of various body systems. In the pathogenesis of glaucoma, it is customary to distinguish a dystrophic concept, which considers primary open-angle glaucoma as a result of dystrophic changes in the connective tissue, as well as in the endothelial lining of the trabeculae and Schlemm′s canal, especially destructive changes in mitochondria and the alteration of their functional activity. A vascular concept is also distinguished. According to this concept, the central link in the pathogenesis of glaucoma is circulatory disorder in the ciliary vessels, ocular artery, and major vessels of the head and neck, it can be assumed that osteopathic correction in the treatment of patients with open-angle glaucoma will be pathogenetically substantiated and will have a positive effect on intraocular pressure and trophicity of the optic nerve. The goal of research — to study the influence of in osteopathic correction on the nature of unoperated glaucoma (stage IIA) and to substantiate the possibility of using osteopathic correction in the complex treatment of patients with this pathology.Materials and methods. A prospective controlled randomized study was conducted at 52 city polyclinics, branch 3, Moscow, from January 2018 to January 2019. 40 patients (70 eyes) aged 50 to 75 years with primary open-angle glaucoma IIA stage were examined. At this stage of the disease, patients most often seek medical care and the issue of conservative management is primarily considered. All patients were divided into two groups of 20 people: the main group and the control group. The treatment in the main group included hypotensive drug therapy and osteopathic correction. Patients of the control group received only drug therapy. All patients underwent ophthalmic (visometry, tonometry, perimetry) and osteopathic examination twice: before the treatment and after 3 months.Results. For patients with primary open-angle IIA non-operated glaucoma, regional (most often regions of the head, neck, dura mater) and local (abdominal diaphragm, iliac bones, hip and knee joints) somatic dysfunctions were the most typical. In the main group a statistically significant decrease in the frequency and severity of dysfunctions at all levels was stated. Also, in patients receiving osteopathic correction, a significant decrease in the level of intraocular pressure and perimetric indices was noted. In patients of the control group, no reliable changes in these indicators were obtained.Conclusion. The results obtained indicate that osteopathic correction is clinically effective in the complex treatment of patients with primary open-angle II A glaucoma.


1990 ◽  
Vol 4 (1) ◽  
pp. 1 ◽  
Author(s):  
In Seop Lee ◽  
Young Suk Yu ◽  
Dong Myung Kim ◽  
Dong Ho Youn ◽  
Jin Q Kim

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