Qualitative and quantitative cuanyes of lipids in experimental peritonitis

1998 ◽  
Vol 79 (5) ◽  
pp. 382-387
Author(s):  
V. A. Trofimov ◽  
A. P. Vlasov ◽  
М. М. Minnebaev
Keyword(s):  
Fatty Acids ◽  
Blood Plasma ◽  
Free Fatty Acids ◽  
Considerable Increase ◽  
Membrane Lipids ◽  
Qualitative And Quantitative ◽  
Lipid Disorders ◽  
Tissue Lipids ◽  
Organ Level ◽  
Adequate Method

The considerable increase of the free fatty acids part, phospholipid lysophorms and the decrease of the main membrane lipids level are found in tissue lipids spectrum in peritonitis. The changes in organ li pids composition in many cases are correlated with those in blood plasma. Hence, the complex estimation of blood li pids is an adequate method of determining the biochemical lipid disorders intensity on the organ level.

scholarly journals FadD Is Required for Utilization of Endogenous Fatty Acids Released from Membrane Lipids

2011 ◽  
Vol 193 (22) ◽  
pp. 6295-6304 ◽  
Author(s):  
Ángel Pech-Canul ◽  
Joaquina Nogales ◽  
Alfonso Miranda-Molina ◽  
Laura Álvarez ◽  
Otto Geiger ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Stationary Phase ◽  
Free Fatty Acids ◽  
Sinorhizobium Meliloti ◽  
Membrane Lipids ◽  
Membrane Phospholipids ◽  
Content Type ◽  
Fatty Acid Transporter ◽  
In The Wild

FadD is an acyl coenzyme A (CoA) synthetase responsible for the activation of exogenous long-chain fatty acids (LCFA) into acyl-CoAs. Mutation offadDin the symbiotic nitrogen-fixing bacteriumSinorhizobium melilotipromotes swarming motility and leads to defects in nodulation of alfalfa plants. In this study, we found thatS. melilotifadDmutants accumulated a mixture of free fatty acids during the stationary phase of growth. The composition of the free fatty acid pool and the results obtained after specific labeling of esterified fatty acids with a Δ5-desaturase (Δ5-Des) were in agreement with membrane phospholipids being the origin of the released fatty acids.Escherichia colifadDmutants also accumulated free fatty acids released from membrane lipids in the stationary phase. This phenomenon did not occur in a mutant ofE. coliwith a deficient FadL fatty acid transporter, suggesting that the accumulation of fatty acids infadDmutants occurs inside the cell. Our results indicate that, besides the activation of exogenous LCFA, in bacteria FadD plays a major role in the activation of endogenous fatty acids released from membrane lipids. Furthermore, expression analysis performed withS. melilotirevealed that a functional FadD is required for the upregulation of genes involved in fatty acid degradation and suggested that in the wild-type strain, the fatty acids released from membrane lipids are degraded by β-oxidation in the stationary phase of growth.

scholarly journals Acquisition of membrane lipids by differentiating glyoxysomes: role of lipid bodies.

1991 ◽  
Vol 115 (4) ◽  
pp. 995-1007 ◽  
Author(s):  
K D Chapman ◽  
R N Trelease
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Cotton Seed ◽  
Membrane Lipids ◽  
Nonpolar Lipid ◽  
Lipid Bodies ◽  
Membrane Phospholipids ◽  
Free System ◽  
Nonpolar Lipids

Glyoxysomes in cotyledons of cotton (Gossypium hirsutum, L.) seedlings enlarge dramatically within 48 h after seed imbibition (Kunce, C.M., R.N. Trelease, and D.C. Doman. 1984. Planta (Berl.). 161:156-164) to effect mobilization of stored cotton-seed oil. We discovered that the membranes of enlarging glyoxysomes at all stages examined contained a large percentage (36-62% by weight) of nonpolar lipid, nearly all of which were triacylglycerols (TAGs) and TAG metabolites. Free fatty acids comprised the largest percentage of these nonpolar lipids. Six uncommon (and as yet unidentified) fatty acids constituted the majority (51%) of both the free fatty acids and the fatty acids in TAGs of glyoxysome membranes; the same six uncommon fatty acids were less than 7% of the acyl constituents in TAGs extracted from cotton-seed storage lipid bodies. TAGs of lipid bodies primarily were composed of palmitic, oleic, and linoleic acids (together 70%). Together, these three major storage fatty acids were less than 10% of both the free fatty acids and fatty acids in TAGs of glyoxysome membranes. Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) constituted a major portion of glyoxysome membrane phospholipids (together 61% by weight). Pulse-chase radiolabeling experiments in vivo clearly demonstrated that 14C-PC and 14C-PE were synthesized from 14C-choline and 14C-ethanolamine, respectively, in ER of cotyledons, and then transported to mitochondria; however, these lipids were not transported to enlarging glyoxysomes. The lack of ER involvement in glyoxysome membrane phospholipid synthesis, and the similarities in lipid compositions between lipid bodies and membranes of glyoxysomes, led us to formulate and test a new hypothesis whereby lipid bodies serve as the dynamic source of nonpolar lipids and phospholipids for membrane expansion of enlarging glyoxysomes. In a cell-free system, 3H-triolein (TO) and 3H-PC were indeed transferred from lipid bodies to glyoxysomes. 3H-PC, but not 3H-TO, also was transferred to mitochondria in vitro. The amount of lipid transferred increased linearly with respect to time and amount of acceptor organelle protein, and transfer occurred only when lipid body membrane proteins were associated with the donor lipid bodies. 3H-TO was transferred to and incorporated into glyoxysome membranes, and then hydrolyzed to free fatty acids. 3H-PC was transferred to and incorporated into glyoxysome and mitochondria membranes without subsequent hydrolysis. Our data are inconsistent with the hypothesis that ER contributes membrane lipids to glyoxysomes during postgerminative seedling growth.(ABSTRACT TRUNCATED AT 400 WORDS)

The association between depot fat mobilization and the presence of xanthophyll in the plasma of normal sheep

1965 ◽  
Vol 64 (2) ◽  
pp. 273-278 ◽  
Author(s):  
D. S. P. Patterson
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Blood Plasma ◽  
Free Fatty Acids ◽  
Fat Depots ◽  
Green Colour ◽  
Chromatographic Methods ◽  
Fat Mobilization

1. Blood plasma of normal fed sheep is not pigmented but on starvation the plasma assumes a yellow to green colour. Unlike the pigments of the so-called yellow fatted sheep, the pigmentation disappears on refeeding.2. Using spectrophotometric and chromatographic methods, this pigment has been identified as xanthophyll (lutein).3. The xanthophyll content of plasma is related to the plasma non-esterified fatty acid (N.E.F.A.) concentration and it therefore appears that the pigment is mobilized from the fat depots together with the free fatty acids. Maximum levels of about 60μg./100 ml. xanthophyll are observed in sheep plasma after 4 days starvation.

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