scholarly journals Post-stress chemosignals affect cells from immunocompetent organs in laboratory mice of three inbred strains

2008 ◽  
Vol 6 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Eugene V Daev ◽  
Boris P Surinov ◽  
Anna V Dukelskaya
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Physiological State ◽  
Inbred Strains ◽  
Laboratory Mice ◽  
Immunocompetent Organs ◽  
Dividing Cells ◽  
First Time ◽  
Mitotic Disturbances ◽  
Post Stress

Quantity of antibody producing cells and changes in bone marrow dividing cells of mouse males were studied after the exposure with chemosignals from intact or stressed donor mouse males. Inbred CBA, BALB/c and C57BL/6 strains were used. It is shown that excreted volatiles decrease quantity of antibody producing cells in spleen and at the same time raise the level of mitotic disturbances in bone marrow cells of recipient mice. Pheromone effect depends on genotype and physiological state of the recipients. For the first time we describe here the influence of mouse female pheromone 2,5-dimethylpyrazine on analyzed features. Biological meaning of the discovered effects is discussed.

Morphofunctional features of proliferating cells exposed to PSMA peptide

2021 ◽  
Author(s):  
N.A. Mikheeva ◽  
E.P. Drozhdina ◽  
N.A. Kurnosova
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Laboratory Animals ◽  
Laboratory Mice ◽  
Proliferating Cells ◽  
Red Bone Marrow ◽  
Toxic Damage ◽  
Dividing Cells ◽  
Marrow Cells

The effect of the synthetic PSMA peptide on dividing cells of laboratory animals was studied. The experiment was carried out on male white laboratory mice of the BALB/c-line. The toxic effect of PSMA peptidi was evaluated at therapeutic (1.4 μg / kg of animal weight or 0.04 μg / animal) and subtoxic (140 μg / kg of animal weight or 4.0 μg / animal) doses. The cytotoxic effect of PSMA peptide on red bone marrow cells and cambial intestinal cells of the of laboratory mice was determined. A decrease in the proliferative activity of the colon crypt cells was revealed upon administration of a subtoxic dose of the PSMA peptide and there were no signs of toxic damage to the red bone marrow cells of animals. Key words: toxicity, proliferation, synthetic peptides, mitotic index, micronucleus test.

scholarly journals Response of immunocompetent cells of bone marrow and spleen of mouse males of several strains to stress and to pyrazine containing chemosignals

2012 ◽  
Vol 10 (2) ◽  
pp. 14-20 ◽  
Author(s):  
Eugene V Daev ◽  
Boris P Surinov ◽  
Anna V Dukelskaya
Keyword(s):  
Bone Marrow ◽  
Immune Suppression ◽  
Food Production ◽  
Bone Marrow Cells ◽  
Pyrazine Ring ◽  
Mouse Genotype ◽  
Dividing Cells ◽  
Different Strains ◽  
Mitotic Disturbances ◽  
Wide Usage

The quantity of antibody producing cells and mitotic disturbances in dividing bone marrow cells of mice were studied after exposure of animals to a physical stressor or various pyrazinecontaining chemosignals. Several different strains of mice were used. We demonstrate that immune suppression and destabilization of the chromosome apparatus in dividing cells depend on: а) mouse genotype and b) side chains position  in the pyrazine ring. Importance of this effects in the light of wide usage of pyrazine containing substances in perfume industry, food production and pharmacology is discussed.

scholarly journals Frequencies of mitogen-reactive B cells in the mouse. I. Distribution in different lymphoid organs from different inbred strains of mice at different ages

1977 ◽  
Vol 145 (6) ◽  
pp. 1511-1519 ◽  
Author(s):  
J Andersson ◽  
A Coutinho ◽  
F Melchers
Keyword(s):  
Bone Marrow ◽  
B Cells ◽  
Thoracic Duct ◽  
Peripheral Blood ◽  
Bone Marrow Cells ◽  
Inbred Strains ◽  
Mesenteric Lymph Nodes ◽  
Inbred Strains Of Mice ◽  
Old Mice

Frequencies of mitogen-reactive B cells have been determined in vitro under culture conditions which allow every growth-inducible B cell to grow and mature into a clone of Ig-secreting PFC. The frequencies of LPS-reactive B cells in the spleen of 6- to 8-wk old mice were between 1 in 3 and 1 in 10 splenic B cells from the following inbred strains of mice: C3H/Tif; BALB/c; BALB/c ν/ν; C57BL/6J; DBA/2J; C57BL/6J x DBA/(2J)F(1); and CBA and A/J. Very similar frequencies are found for lipoprotein-reactive B cells in BALB/c, BALB/c ν/ν, C3H/Tif, and C3H/HeJ mice. No LPS-reactive cells but normal frequencies of lipoprotein-reactive cells were found in C3H/HeJ mice, genetically nonreactive to LPS. SJL mice had significantly lower frequencies of LPS- and of lipoprotein-reactive B cells (1 in approximately 30 B cells). The number of LPS- and of lipoprotein-reactive B cells in spleen was dependent upon the age of the mouse. Newborn spleen contained approximately 10 percent of the number of reactive cells found at 6- to 8-wk of age. From there the frequencies declined again to drop below 5 percent of the maximal number at ages beyond 11 mo. LPS-reactive B cells yielding IgM- and IgG-PFC responses could be found in mesenteric lymph nodes, bone marrow, thymus, thoracic duct, and peripheral blood of 6- to 8-wk old mice. Their frequencies were one in three to five lymph node cells, 1 in 50 to 100 bone marrow cells, one in 10(5) thymus cells, and 1 in 20 to 40 thoracic duct or peripheral blood cells.

INDUCTION OF ANEUPLOIDY IN MOUSE BONE MARROW CELLS WITH DIETHYLSTILBESTROL-DIPHOSPHATE

1974 ◽  
Vol 16 (4) ◽  
pp. 831-835 ◽  
Author(s):  
C. L. Chrisman ◽  
L. L. Hinkle
Keyword(s):  
Bone Marrow ◽  
Chromosome Number ◽  
Adult Male ◽  
Bone Marrow Cells ◽  
Single Injection ◽  
Inbred Strains ◽  
Mouse Bone Marrow ◽  
Post Injection ◽  
Male Mice ◽  
Mouse Bone Marrow Cells

Bone marrow was collected from adult male mice derived from four way crosses between inbred strains. Sixty males were included in an experiment in which treatment animals were given a single injection of 1, 10, or 100 μg/g diethylstilbestrol-diphosphate (DES-dp) and killed 6, 24, or 48 h post injection. An additional 20 males were given one injection of the drug daily for 5 days and killed 6 h after the last injection. Chromosome number determinations from 25 metaphase plates of 60 treatment animals were compared with counts from 20 controls. Analysis of the data revealed that dose and duration of exposure to DES-dp were both influential in producing aneuploid cells.

scholarly journals Predator odor induces genome instability in the mouse bone marrow cells

2017 ◽  
Vol 15 (1) ◽  
pp. 4
Author(s):  
Timofey S Glinin ◽  
Polina A Starshova ◽  
Victoria A Shubina ◽  
Margarita V Anisimova ◽  
Anton A Bondarenko ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Genome Instability ◽  
Genetic Effect ◽  
Predator Odor ◽  
Mouse Bone Marrow ◽  
Predator Species ◽  
Genome Damage ◽  
Dividing Cells ◽  
Marrow Cells

Background. Long coevolution of prey and predator species of mammals creates specific mechanisms of their interaction, e. g. prey’s innate behavior aversive to the predator odor. However, little is known about genetic responses in the prey organism. We assessed genome instability of the bone marrow cells in mice affected by the cat’s odor influence, and proposed pathway of such action. Materials and methods. CBA mouse males were exposed to volatiles from adult cat urine for 2 or 24 hours. To estimate the genetic effect, ana-telophase method of chromosome aberration analysis and comet assay were used. The level of corticosterone was also measured after the exposure for 30 or 60 minutes. Results. The exposure to cat’s urine volatiles for 2 hours induced damage of DNA in bone marrow cells of the mouse males as was shown by the DNA comet analysis. The exposure for 24 hours elevated the frequency of chromosome aberrations in mitotically dividing cells at ana-telophase stage. No significant changes were found in the level of corticosterone in the peripheral blood. Conclusion. We have shown that volatile chemosignals from predator’s urine induce genomic instability in bone marrow cells of a prey. The hormonal pathway of such influence is still unknown. Intraorganismic paths leading to genome damage are discussed as well as far consequences of discovered effects.

scholarly journals Development of hematopoietic spleen colonies in nonirradiated genetically normal mice

Blood ◽  
1977 ◽  
Vol 50 (6) ◽  
pp. 1121-1127 ◽  
Author(s):  
Y Kitamura ◽  
M Tamai ◽  
Y Miyano ◽  
M Shimada
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Erythroid Cell ◽  
Hematopoietic Stem ◽  
F1 Hybrid ◽  
C57bl Mice ◽  
Dividing Cells ◽  
Hybrid Mice ◽  
Suitable Environment ◽  
Marrow Cells

Abstract The question as to whether prior irradiation or injection of cytotoxic drugs is essential for the development of spleen colonies was examined in genetically normal mice. Mixtures of lymph node and bone marrow cells from C57BL mice were injected into (C57BL X CBA-T6T6) F1 hybrid mice without pretreatment. Hematopoietic nodules were observed in the spleens of F1 hybrid mice killed 18 days after injection. The average number of nodules increased linearly with increased numbers of injected bone marrow cells. Hematopoietic stem cells (CFU-S) and dividing cells in the nodules were shown to be of C57BL origin. Histologic examination showed that erythroid cell colonies predominated over granulocytic cell colonies. These results suggest that any kind of treatment that causes the depletion of CFU-S in the spleen of hosts would provide a suitable environment for the production of colonies by transplanted CFU-S.

Hematological measurements in rats flown on Spacelab shuttle, SL-3

1987 ◽  
Vol 252 (2) ◽  
pp. R216-R221 ◽  
Author(s):  
R. D. Lange ◽  
R. B. Andrews ◽  
L. A. Gibson ◽  
C. C. Congdon ◽  
P. Wright ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Life Science ◽  
Bone Marrow Cells ◽  
Cell Mass ◽  
Red Cell ◽  
Cell Counts ◽  
Animal Bone ◽  
Red Cell Mass ◽  
And Control ◽  
First Time

Previous studies have shown that a decrease in red cell mass occurs in astronauts, and some studies indicate a leukocytosis occurs. A life science module housing young and mature rats was flown on shuttle mission Spacelab 3 (SL-3), and the results of hematology studies of flight and control rats are presented. Statistically significant increases in the hematocrit, red blood cell counts, and hemoglobin determinations, together with a mild neutrophilia and lymphopenia, were found in flight animals. No significant changes were found in bone marrow and spleen cell differentials or erythropoietin determinations. Clonal assays demonstrated an increased erythroid colony formation of flight animal bone marrow cells at erythropoietin doses of 0.02 and 1.0 U/ml but not 0.20 U/ml. These results agree with some but vary from other previously published studies. Erythropoietin assays and clonal studies were performed for the first time.

scholarly journals PROLIFERATION OF DONOR MARROW AND THYMUS CELLS IN THE MYELOID AND LYMPHOID ORGANS OF IRRADIATED SYNGENEIC HOST MICE

1973 ◽  
Vol 137 (2) ◽  
pp. 543-546 ◽  
Author(s):  
Akikazu Takada ◽  
Yumiko Takada
Keyword(s):  
Bone Marrow ◽  
Lymph Nodes ◽  
Bone Marrow Cells ◽  
The Other ◽  
Host Bone ◽  
Donor Type ◽  
Dividing Cells ◽  
Spleen And Lymph Nodes ◽  
Thymus Cells ◽  
Marrow Cells

CBA/HT6T6 bone marrow cells (1 x 107) or CBA/H bone marrow cells (1 x 107) plus CBA/HT6T6 thymus cells (5 x 107) were injected intravenously into lethally (800 R) irradiated CBA/H mice. Chromosome analyses of dividing cells in the host lymphoid and myeloid organs were performed at intervals after irradiation. Donor marrow cells settled and proliferated in the host bone marrow, spleen, and lymph nodes soon after injection, but donor marrow cells did not proliferate in the host thymus until day 10; then host-type cells were quickly replaced by donor-type cells in the thymus by day 20. On the other hand, donor thymus cells settled and proliferated in the host thymus and lymph nodes soon after injection but they gradually disappeared from these organs. On day 20, a few donor-type dividing cells (of thymus origin) were found in the host lymphoid and myeloid organs.

scholarly journals Development of hematopoietic spleen colonies in nonirradiated genetically normal mice

Blood ◽  
1977 ◽  
Vol 50 (6) ◽  
pp. 1121-1127
Author(s):  
Y Kitamura ◽  
M Tamai ◽  
Y Miyano ◽  
M Shimada
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Erythroid Cell ◽  
Hematopoietic Stem ◽  
F1 Hybrid ◽  
C57bl Mice ◽  
Dividing Cells ◽  
Hybrid Mice ◽  
Suitable Environment ◽  
Marrow Cells

The question as to whether prior irradiation or injection of cytotoxic drugs is essential for the development of spleen colonies was examined in genetically normal mice. Mixtures of lymph node and bone marrow cells from C57BL mice were injected into (C57BL X CBA-T6T6) F1 hybrid mice without pretreatment. Hematopoietic nodules were observed in the spleens of F1 hybrid mice killed 18 days after injection. The average number of nodules increased linearly with increased numbers of injected bone marrow cells. Hematopoietic stem cells (CFU-S) and dividing cells in the nodules were shown to be of C57BL origin. Histologic examination showed that erythroid cell colonies predominated over granulocytic cell colonies. These results suggest that any kind of treatment that causes the depletion of CFU-S in the spleen of hosts would provide a suitable environment for the production of colonies by transplanted CFU-S.

scholarly journals THE INFLUENCE OF THE BONE MARROW XENOTRANSPLANTATION ON PRODUCING OF MEDIATORS BY ITS NEUROAMINE-PRODUCING CELLS

2018 ◽  
Vol 26 (4) ◽  
pp. 7-10
Author(s):  
O. V. Vorob’Eva ◽  
L. A. Lubovtseva
Keyword(s):  
Bone Marrow ◽  
Cell Suspension ◽  
Bone Marrow Cells ◽  
Laboratory Mice ◽  
Domestic Cats ◽  
Tail Vein ◽  
Early Stages ◽  
A Cell ◽  
Quantitative Indicators ◽  
Marrow Cells

The quantitative indicators of neuroamine-producing cells and the content of neuromediators in them were analyzed, the correlation of indicators of their production after bone marrow xenotransplantation was studied. The experiments were conducted on white laboratory mice, which were injected with a cell suspension obtained from a domestic cats into the tail vein. In the early stages of the experiment, the number of neuroamine-producing cells increases, with an increase in the content of neurotransmitters in them. In the subsequent periods of the experiment, their number decreases and the production of all neuromediators (catecholamines, serotonin, histamine) sharply decrease. The formation of lipocytes in the transplanted tissue is showed. Correlations between the levels of neuromediators in neuroamine-producing bone marrow cells also change. Thus, bone marrow xenotransplantation leads to a decrease in the number and production of neuromediators by neuroamine-producing cells within two days.

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