INDUCTION OF ANEUPLOIDY IN MOUSE BONE MARROW CELLS WITH DIETHYLSTILBESTROL-DIPHOSPHATE

Bone marrow was collected from adult male mice derived from four way crosses between inbred strains. Sixty males were included in an experiment in which treatment animals were given a single injection of 1, 10, or 100 μg/g diethylstilbestrol-diphosphate (DES-dp) and killed 6, 24, or 48 h post injection. An additional 20 males were given one injection of the drug daily for 5 days and killed 6 h after the last injection. Chromosome number determinations from 25 metaphase plates of 60 treatment animals were compared with counts from 20 controls. Analysis of the data revealed that dose and duration of exposure to DES-dp were both influential in producing aneuploid cells.