scholarly journals Determination of Phylogenetic Relationships of Five Species of Centaurea Genus by Using trnT-L-F cpDNA Region

2017 ◽  
pp. 52-59 ◽  
Author(s):  
Ayşenur KALMER ◽  
Ayten DİZKIRICI TEKPINAR
Keyword(s):  
Phylogenetic Relationships ◽  
Cpdna Region

Species diversity and phylogeny of fleas of small terrestrial mammals in the forests of the Central Highlands of Madagascar

Zootaxa ◽  
2018 ◽  
Vol 4399 (2) ◽  
pp. 181
Author(s):  
MIREILLE HARIMALALA ◽  
ADELAÏDE MIARINJARA ◽  
JEAN-BERNARD DUCHEMIN ◽  
TOJO RAMIHANGIHAJASON ◽  
SEBASTIEN BOYER
Keyword(s):  
Bayesian Methods ◽  
Phylogenetic Relationships ◽  
12S Rrna ◽  
Neighbor Joining ◽  
Classification Schemes ◽  
Terrestrial Mammals ◽  
The Family ◽  
Current Classification ◽  
Central Highlands

Fleas are holometabolous insects forming the order of Siphonaptera. Some studies have been carried out on biology and systematic of Malagasy fleas, but little is known about their phylogenetic relationships. In this study, we focused on flea species occurring in the forests of the Central Highlands and also, on the determination of their phylogenetic relationships. Three families, five genera and thirteen species were identified. The family Pulicidae includes four species (Centetipsylla madagascariensis Rothschild, Synopsyllus fonquerniei Wagner & Roubaud, S. estradei Klein and S. robici Klein); Leptopsyllidae has eight species (Paractenopsyllus vauceli Klein, P. petiti Klein, P. viettei Klein, P. grandidieri Klein, P. goodmani Duchemin, P. rouxi Duchemin, P. raxworthyi Duchemin & Ratovonjato and Tsaractenus rodhaini Duchemin), and Ctenophtalmidae one species (Dinopsyllus brachypecten Smit). All are endemic to Madagascar and each differs geographically. Flea phylogenetic relationships were inferred using four molecular markers (ITS2, mtCOII, 16SrRNA and 12S rRNA) and using Neighbor-Joining, Maximum Parsimony and Bayesian methods with addition of Genbank sequences of exotic species. The Family Pulicidae was monophyletic while the families Leptopsyllidae and Ctenophtalmidae were paraphyletic. Malagasy fleas are homogeneous and all species adhere to current classification schemes. 

A PCR primer set for determination of phylogenetic relationships of Bursaphelenchus species within the xylophilus group

Nematology ◽  
2002 ◽  
Vol 4 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Kazuyoshi Futai
Keyword(s):  
North America ◽  
Phylogenetic Relationships ◽  
Coniferous Forest ◽  
The Other ◽  
Coi Gene ◽  
Eurasian Continent ◽  
Dna Base ◽  
Base Sequences ◽  
Free Living Nematode

AbstractThe phylogenetic relationships of Bursaphelenchus conicaudatus with B. abruptus, B. fraudulentus, B. mucronatus and B. xylophilus species were analysed based on the DNA base sequences of 18S, 5.8S, ITS1 and ITS2 of rDNA and the partial code of mitochondrial cytochrome oxidase subunit I (COI) gene. The monophyly of B. abruptus and the other species in the xylophilus group was not supported, and B. abruptus could be excluded from the xylophilus group. The other species, i.e ., B. conicaudatus, B. fraudulentus, B. mucronatus and B. xylophilus were assumed to be monophyletic. The ancestor of these four species is assumed to have originated in the eastern part of the Eurasian continent as a free-living nematode inhabiting broad-leaved trees. First, B. conicaudatus branched from the ancestor in eastern Asia. Then B. fraudulentus and B. mucronatus separated from the ancestor. B. mucronatus changed its host from broad-leaved trees to conifers and spread throughout the coniferous forest over the Eurasian continent and North America. B. xylophilus might originate from a population of B. mucronatus remaining in North America after B. mucronatus had diversified.

scholarly journals TrnL-trnfF cpDNA polymorphism in some representatives of the genus Betula

2021 ◽  
Vol 35 ◽  
pp. 00017
Author(s):  
Svetlana Medvedeva ◽  
Olga Cherepanova ◽  
Olga Tolkach ◽  
Vasiliy Ponomarev ◽  
Galina Malosieva
Keyword(s):  
Central Asia ◽  
Boreal Forests ◽  
Population Based ◽  
Forest Steppe ◽  
Correct Identification ◽  
The Urals ◽  
Cpdna Region ◽  
Mountain System ◽  
Single Marker

We present preliminary results of the trnL-trnF cpDNA region analysis for some representatives of the g. Betula, out of which B. procurva can be considered the most interesting. The disjunctive range of this species is confined to the mountain system in southeast Central Asia (Pamir-Alai). The occurrence of the birch is isolated from the main range in the mountainous part of the Urals, in the boreal forests zone and in the Trans-Ural forest-steppe. Due to high interspecific hybridization and population variability of the g. Betula, doubts were raised about the correct identification of the representatives of B. procurva. The studied genetic variability and population structure of B. procurva, B. nana, B. pubescens, B. turkestanica, B. tianshanica and B. pendula species indicate active introgression and hybridization processes. Polymorphism in all groups is significantly reduced, increasing slightly when comparing the most distant groups. The matching of the B. procurva cpDNA haplotypes (GBS) with B. pendula, B. nana, and B. pubescens shows that this trnL-trnF cpDNA site cannot be used for molecular identification of birch species by barcoding as a single marker, but this marker use is possible for determination of certain B. procurva population. Based on the studied cpDNA region (trnL-F) we found a clear geographic subdivision in B. procurva populations of the Trans-Urals and Central Asia,.

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