scholarly journals MYOCARDIAL PATHOMORPHOLOGY IN DISTANT BONE TISSUE DAMAGE AND APPLICATION OF EXOSOMES OF MULTIPOTENT STROMAL CELLS

2021 ◽  
pp. 81-81
Author(s):  
S.A. Kuzkin ◽  
I.V. Mayborodin ◽  
E.V. Koldysheva ◽  
M.G. Klinnikova ◽  
V.I. Mayborodina
Keyword(s):  
Bone Tissue ◽  
Stromal Cells ◽  
Tissue Damage ◽  
Multipotent Stromal Cells

scholarly journals First Experimental Study of the Influence of Extracellular Vesicles Derived from Multipotent Stromal Cells on Osseointegration of Dental Implants

2021 ◽  
Vol 22 (16) ◽  
pp. 8774
Author(s):  
Igor Maiborodin ◽  
Aleksandr Shevela ◽  
Vera Matveeva ◽  
Vitaly Morozov ◽  
Michael Toder ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Dental Implants ◽  
Extracellular Vesicles ◽  
Stromal Cells ◽  
Soft Tissues ◽  
Control Group ◽  
Complete Fusion ◽  
Multipotent Stromal Cells ◽  
Tibial Condyle ◽  
Histologic Pattern

Herein, the aim was to study the state of the bone tissue adjacent to dental implants after the use of extracellular vesicles derived from multipotent stromal cells (MSC EVs) of bone marrow origin in the experiment. In compliance with the rules of asepsis and antiseptics under general intravenous anesthesia with propofol, the screw dental implants were installed in the proximal condyles of the tibia of outbred rabbits without and with preliminary introduction of 19.2 μg MSC EVs into each bone tissue defect. In 3, 7, and 10 days after the operation, the density of bone tissue adjacent to different parts of the implant using an X-ray unit with densitometer was measured. In addition, the histological examinations of the bone site with the hole from the removed device and the soft tissues from the surface of the proximal tibial condyle in the area of intra-bone implants were made. It was found out that 3 days after implantation with the use of MSC EVs, the bone density was statistically significantly higher by 47.2% than after the same implantation, but without the injection of MSC EVs. It is possible that as a result of the immunomodulatory action of MSC EVs, the activity of inflammation decreases, and, respectively, the degree of vasodilation in bones and leukocyte infiltration of the soft tissues are lower, in comparison with the surgery performed in the control group. The bone fragments formed during implantation are mainly consolidated with each other and with the regenerating bone. Day 10 demonstrated that all animals with the use of MSC EVs had almost complete fusion of the screw device with the bone tissue, whereas after the operation without the application of MSC EVs, the heterogeneous histologic pattern was observed: From almost complete osseointegration of the implant to the absolute absence of contact between the foreign body and the new formed bone. Therefore, the use of MSC EVs during the introduction of dental implants into the proximal condyle of the tibia of rabbits contributes to an increase of the bone tissue density near the device after 3 days and to the achievement of consistently successful osseointegration of implants 10 days after the surgery.

scholarly journals Morphology of the Myocardium after Experimental Bone Tissue Trauma and the Use of Extracellular Vesicles Derived from Mesenchymal Multipotent Stromal Cells

2021 ◽  
Vol 11 (11) ◽  
pp. 1206
Author(s):  
Igor Maiborodin ◽  
Marina Klinnikova ◽  
Sergey Kuzkin ◽  
Vitalina Maiborodina ◽  
Sergey Krasil’nikov ◽  
...  
Keyword(s):  
Blood Flow ◽  
Blood Vessels ◽  
Bone Tissue ◽  
Extracellular Vesicles ◽  
Stromal Cells ◽  
The Body ◽  
Simultaneous Increase ◽  
Multipotent Stromal Cells ◽  
Vascular Walls ◽  
Mesenchymal Multipotent Stromal Cells

The effect of extracellular vesicles (EVs) of various origins on the heart structures in the time of health and disease has been well studied. At the same time, data on the distribution of EVs throughout the body after introduction into the tissues and the possibility of the influence of these EVs on organs distant from the injection site are practically absent. It is also necessary to note a certain inconsistency in the results of various researchers: from articles on the direct absorption of EVs derived from mesenchymal multipotent stromal cells (MSC EVs) by cardiomyocytes to the data that the heart is inherently immune to drug delivery mediated by nanoparticles. In this regard, the morphological changes in the myocardium of outbred rabbits of both sexes weighing 3–4 kg were studied at various times after experimental trauma of the bone tissue in the proximal condyle of the tibia (PCT) and the use of MSC EVs. As a result of modeling the PCT defect, rabbits develop myocardial edema in the heart muscle by the 3rd day, their lymphatic vessels expand, and then, on the 7th day, the blood vessels become dilated. In the myocardium, the relative and absolute contents of neutrophils, erythrocytes, and macrophages increase, but the percentage of lymphocytes decreases. By day 10, almost all of these changes return to their initial values. The detected transformations of the myocardium are most likely due to the ingress of detritus with the blood flow from the PCT. The use of MSC EVs to influence the regeneration of damaged tissue of PCT promotes earlier dilatation of the blood vessels of the heart with pronounced diapedesis of erythrocytes or even hemorrhages, prolongation of edema, the formation of blood clots in vessels with obliteration of their lumen, sclerotic transformation of vascular walls and paravascular tissues. In the myocardium, the number density of neutrophils, the percentage of lymphocytes, and neutrophils become smaller, with a simultaneous increase in the relative numbers of erythrocytes and macrophages, and changes in the content of macrophages remained until the end of the observation—up to 10 days after the surgery. The discovered effect of MSC EVs is most likely associated with the suppression of the activity of the inflammatory process in the PCT area, which, in turn, was caused by a longer ingress of detritus with blood flow into the myocardium. The absence of statistically significant differences between changes in the myocardium of the left and right ventricles may indicate that both detritus from the surgical site and MSC EVs affect the heart spreading through the coronary artery system.

Y2O3 Nanoparticles Caused Bone Tissue Damage by Breaking the Intracellular Phosphate Balance in Bone Marrow Stromal Cells

ACS Nano ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. 313-323 ◽  
Author(s):  
Chunyue Gao ◽  
Yi Jin ◽  
Guang Jia ◽  
Xiaomin Suo ◽  
Huifang Liu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Tissue ◽  
Stromal Cells ◽  
Tissue Damage ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Intracellular Phosphate

Isolation of Multipotent Stromal Cells from Mouse Bone Marrow

BIO-PROTOCOL ◽  
2014 ◽  
Vol 4 (4) ◽  
Author(s):  
Aurélie Tormo ◽  
Moutih Rafei ◽  
Jean-François Gauchat
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Mouse Bone Marrow ◽  
Multipotent Stromal Cells

scholarly journals Experimental Type 2 Diabetes Differently Impacts on the Select Functions of Bone Marrow-Derived Multipotent Stromal Cells

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 268
Author(s):  
Jonathan Ribot ◽  
Cyprien Denoeud ◽  
Guilhem Frescaline ◽  
Rebecca Landon ◽  
Hervé Petite ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Bone Marrow ◽  
Stromal Cells ◽  
Adipogenic Differentiation ◽  
Therapeutic Modality ◽  
Multipotent Stromal Cells ◽  
Cell Therapies

Bone marrow-derived multipotent stromal cells (BMMSCs) represent an attractive therapeutic modality for cell therapy in type 2 diabetes mellitus (T2DM)-associated complications. T2DM changes the bone marrow environment; however, its effects on BMMSC properties remain unclear. The present study aimed at investigating select functions and differentiation of BMMSCs harvested from the T2DM microenvironment as potential candidates for regenerative medicine. BMMSCs were obtained from Zucker diabetic fatty (ZDF; an obese-T2DM model) rats and their lean littermates (ZL; controls), and cultured under normoglycemic conditions. The BMMSCs derived from ZDF animals were fewer in number, with limited clonogenicity (by 2-fold), adhesion (by 2.9-fold), proliferation (by 50%), migration capability (by 25%), and increased apoptosis rate (by 2.5-fold) compared to their ZL counterparts. Compared to the cultured ZL-BMMSCs, the ZDF-BMMSCs exhibited (i) enhanced adipogenic differentiation (increased number of lipid droplets by 2-fold; upregulation of the Pparg, AdipoQ, and Fabp genes), possibly due to having been primed to undergo such differentiation in vivo prior to cell isolation, and (ii) different angiogenesis-related gene expression in vitro and decreased proangiogenic potential after transplantation in nude mice. These results provided evidence that the T2DM environment impairs BMMSC expansion and select functions pertinent to their efficacy when used in autologous cell therapies.

scholarly journals Some Special Aspects of Liver Repair after Resection and Administration of Multipotent Stromal Cells in Experiment

Life ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 66
Author(s):  
Igor Maiborodin ◽  
Elena Lushnikova ◽  
Marina Klinnikova ◽  
Swetlana Klochkova
Keyword(s):  
Bone Marrow ◽  
Liver Resection ◽  
Connective Tissue ◽  
Light Microscopy ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Multipotent Stromal Cells ◽  
Rapid Restoration ◽  
Bone Marrow Origin

Changes in rat liver after resection and injection of autologous multipotent mesenchymal stromal cells of bone marrow origin (MSCs) transfected with the GFP gene and cell membranes stained with red-fluorescent lipophilic membrane dye were studied by light microscopy. It was found that after the introduction of MSCs into the damaged liver, their differentiation into any cells was not found. However, under the conditions of MSCs use, the number of neutrophils in the parenchyma normalizes earlier, and necrosis and hemorrhages disappear more quickly. It was concluded that the use of MSCs at liver resection for the rapid restoration of an organ is inappropriate, since the injected cells in vivo do not differentiate either into hepatocytes, into epithelial cells of bile capillaries, into endotheliocytes and pericytes of the vascular membranes, into fibroblasts of the scar or other connective tissue structures, or into any other cells present in the liver.

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