scholarly journals THE EFFECT OF LOW INTENSITY MICROWAVE RADIATION FREQUENCY OF 1 GHZ ON THE FUNCTIONAL STATE OF THE MONONUCLEAR CELLS OF WHOLE BLOOD IN HEALTHY YOUNG PERSONS

2016 ◽  
Vol 6 (№ 4 2016) ◽  
Author(s):  
S.S. Bondar ◽  
I.V. Terekhov
Keyword(s):  
Microwave Radiation ◽  
Functional State ◽  
Whole Blood ◽  
Mononuclear Cells ◽  
Radiation Frequency ◽  
Low Intensity

Dependence of the content of individual molecules in agranucocytes of whole blood at coronary heart disease from the level of phosphorylation of protein kinase r 38 in terms of low intensity microwave radiation

2016 ◽  
Vol 10 (1) ◽  
pp. 0-0 ◽  
Author(s):  
Логаткина ◽  
A. Logatkina ◽  
Бондарь ◽  
S. Bondar ◽  
Терехов ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Protein Kinase ◽  
Signaling Pathway ◽  
Microwave Radiation ◽  
Whole Blood ◽  
Mononuclear Cells ◽  
Protein Kinase R ◽  
Low Intensity ◽  
Mononuclear Leucocytes

Molecular indicators reflecting the states of stress-limiting systems of mononuclear leucocytes in the blood, as well as the effects of low-intensive microwave radiation in patients with coronary artery disease were studied. The work it was evaluated the content in mononuclear leucocytes whole blood of components PI3P/AKT/mTOR/p70S6K1 of signaling pathway, heat shock proteins (HSP27, HSP70, HSP90), the concentra-tion of antioxidants and peroxides depending on the level of phosphorylation of the terminal protein kinase MAPK/SAPK of signaling pathway – p38. The results of the study. It was revealed the dependence of a level of studied factors from the degree of phosphorylation p38 in patients with coronary heart disease. It was defined the 38 p sensitivity to the effects of low-intensity microwave radiation, it is manifested by increased level of phosphorylation in the irradiated cul-tures. This study revealed the sensitivity to low-intensity microwave irradiation of content in the mononuclear cells phosphorylated forms of the protein kinases AMPK, AKT1, p70S6K1, as well as the antioxidant status and protein of p53-dependent initial content in the cell phosphorylated form p38. It was shown a possibility of microwave radiation to reduce the content in the cells of the protein kinase and p70 ACT more pronounced at high levels of phosphorylation p38.

The condition of the production of cytokines, soluble co-stimulatory molecules, intracellular signaling pathways and nitric oxide in patients with coronary heart disease on the background of low-intensity microwave therapy

2016 ◽  
Vol 10 (1) ◽  
pp. 0-0 ◽  
Author(s):  
Аржников ◽  
V. Arzhnikov ◽  
Логаткина ◽  
A. Logatkina ◽  
Бондарь ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Acute Coronary Syndrome ◽  
Heart Disease ◽  
Angina Pectoris ◽  
Microwave Radiation ◽  
Interstitial Fluid ◽  
Radiation Frequency ◽  
Coronary Syndrome ◽  
Low Intensity ◽  
Caspase 1

The concentration of cytokines of the family IL-10 (IL-10, IL-22, IL-24), interferon-gamma, soluble forms of co-stimulatory molecules CD28, CD80, CD152, as well as NO was investigated in patients with coronary heart disease in interstitial fluid. In addition, the level of caspase-1, inducible and endothelial forms of NO synthasis (eNOS, iNOS), protein kinase AKT1 and AMPK and the total antioxidant activity from the cell culture supernantat were evaluatedin the agranulocytic patients. In the current study (in vitro) the impact on production of these mediators of low-intensity microwave radiation frequency of 1000 MHz, generated by an apparatus of low-intensity physiotherapy "Aquaton" was evaluated. Material of this study was whole venous blood of patients with angina pectoris and acute coronary syndrome. The analysis of the results revealed a reduced level of NO, IL-24, as well as increased production of IL-10, IL-22 and the concentration of soluble forms of CD28, CD80, CD152 in patients with coronary artery disease in the interstitial fluid in comparison with practically healthy persons. At a single irradiation of cell cultures whole blood by low-intensity microwave radiation frequency of 1000 MHz in patients with angina pectoris and acute coronary syndrome there were an increasing of NO production by 20,6 and 25,5‰, IL-24 by 18,6 and 33,6‰, and a reduction in intracellular caspase-1 by 32,5 and 25,8‰, as well as increasing of the levels of IL-10 by 3,5 and 3,1‰, IL-22 by 28,7 and 26,5‰ and reduction of the levels of CD28 by 3,6 and 3,9‰, CD80 6,8 and 5,7‰, CD152 by 9,4 and 11,3‰, respectively. In addition, in terms of irradiation there is an increase in interstitial fluid levels of antioxidants by 37,1 and 49,2‰.

Cytokine production by whole blood cells of the reconvalescents of community-acquired pneumonia under the influence of low-intensity microwave irradiation

10.12737/5025 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Никифоров ◽  
V. Nikiforov ◽  
Терехов ◽  
I. Terekhov ◽  
Хадарцев ◽  
...  
Keyword(s):  
Microwave Radiation ◽  
Whole Blood ◽  
Blood Cells ◽  
Cytokine Production ◽  
Cell Activity ◽  
Community Acquired Pneumonia ◽  
Tnf Alpha ◽  
Toll Like Receptor ◽  
Low Intensity ◽  
Whole Blood Cells

The study discusses the effect on the functional state of the whole blood cells of low-intensity microwave radiation. On the model of intercellular interactions in the conditions of spontaneous cell activity and by stimulating cells complex mitogen, the influence of microwave radiation 1000 MHz on production by whole blood cells interleukins: IL-1β, 2, 6, 8, 10, 12, 13, 17A, TNF-alpha, IFN-g, RAIL-1, and soluble forms of toll-like receptor 1, 2, 4, 6 types was studied. The research results show that a single 45 minutes of exposure of microwave radiation of 0.05 PPM microwatt/cm2 has the ability to enhance mitogen-stimulated production of RAIL-1 by 38,7% (p=0.03), to reduce the induced production of IL-1β by 26,3% (p=0,037), IL-8 by 56,2% (p=0.022) and to increase oppressed segment complex mitogen production of IL-10 by 27,8% (p=0,041). The microwave radiation increased spontaneous and mitogen-stimulated expression of TLR, especially TLR1 during the initial low level.

MODELS OF BIOMODULATION IN A MICROPROCESSOR SYSTEM OF SUPER LOW INTENSIVE MICROWAVE RADIATION, INTENDED FOR TRANSFORMATION OF BRAIN NEURODYNAMIC ACTIVITY AND MODIFICATION OF THE FUNCTIONAL STATE OF THE PATIENT

2021 ◽  
pp. 160-168
Author(s):  
Феликс Андреевич Пятакович ◽  
Ольга Владимировна Мевша ◽  
Татьяна Игоревна Якунченко ◽  
Кристина Феликсовна Макконен
Keyword(s):  
Microwave Radiation ◽  
Functional State ◽  
Clinical Medicine ◽  
Interdisciplinary Collaboration ◽  
Power Level ◽  
Experimental Studies ◽  
Physical Nature ◽  
Microprocessor System ◽  
Low Intensity ◽  
The Brain

Еще в 60-70 годы XX-го столетия в экспериментальных исследованиях новосибирских ученых, выполненных под руководством академика В.П.Казначеева, была установлена роль сверхслабых физических полей в развитии цитопатологического действия биологических объектов вирусной природы, агентов химической и физической природы. В последние годы в экспериментальной и клинической медицине наряду с использованием низкоинтенсивных крайне высокочастотных миллиметровых излучений (менее 10 мВт/см) успешно используют СВЧ генераторы сверхнизкой интенсивности сантиметрового диапазона длин волн на уровне мощности, не превышающих 10 мкВт/см. Подобное СВЧ излучение сверхмалой мощности, осуществляемое на частоте колебаний кластеров воды первого порядка, способствует нормализации процессов метаболизма. Настоящее исследование выполнено в соответствии с планами проблемной комиссии по «хронобиологии и хрономедицине» РАН. На основе междисциплинарного сотрудничества была сформирована виртуальная команда исследователей работающих в разных университетах страны и включавшая математиков, системных программистов, инженеров и врачей-исследователей. При этом для реализации целей и задач исследования использовалась, так называемая, линейная модель движения потока информации из научной лаборатории в клиническую практику (from bench to bedside). В связи с чем, нами был реализован проект, направленный на разработку технической системы модульного типа, предназначенной для генерации СВЧ излучения с заданными свойствами. В микроконтроллере СВЧ генератора был запрограммирован цифровой аналог сигналов отражающих нейродинамические процессы мозга. В настоящей статье показана электрофизиологическая эффективность сверхнизко интенсивных излучений сантиметрового диапазона длин волн модулированных низкочастотными сигналами подобных ритму ЭЭГ и с циклической изменяемостью скважности. В клинических исследованиях у больных сахарным диабетом II с сопутствующей гипертонической болезнью на основе теста Спилбергера показана трансформация патологической нейродинамической активности мозга с последующей модификацией функционального состояния пациента. Рассмотрены результаты не эффективного воздействия, эффективного воздействия и успешного воздействия с изменениями динамики уровня пластичности нейродинамической активности мозга Back in the 60s-70s of the 20th century, in the experimental studies of Novosibirsk scientists, carried out under the leadership of Academician V.P. Kaznacheev, the role of super weak physical fields in the development of the cytopathological action of biological objects of viral nature, agents of chemical and physical nature was established. In recent years, in experimental and clinical medicine, along with the use of low-intensity extremely high-frequency millimeter radiation (less than 10 mW / cm), microwave generators of super low intensity of the centimeter wavelength range have been successfully used at a power level not exceeding 10 μW/cm. Such super low-power microwave radiation, is realized at the frequency of vibrations of first-order water clusters, contributes to the normalization of metabolic processes. The present study was work out in accordance with the plans of the problem commission on "chronobiology and chronomedicine" of the Russian Academy of Sciences. On the basis of interdisciplinary collaboration, a virtual team of researchers working in different universities in the country was formed and included mathematicians, systems programmers, engineers and medical researchers. At the same time, to implement the goals and objectives of the study, the so-called linear model of the flow of information from the scientific laboratory to clinical practice (from bench to bedside) was used. In this connection, we have implemented a project aimed at developing a modular-type technical system designed to generate microwave radiation with desired properties. In the microcontroller of the microwave generator, a digital analogue of signals reflecting the neurodynamic processes of the brain was programmed. This article shows the electrophysiological efficiency of super-low-intensity radiation in the centimeter range of wavelengths modulated by low-frequency signals similar to the EEG rhythm. In experimental studies in patients with diabetes mellitus II with concomitant hypertension, the transformation of the pathological neurodynamic activity of the brain with the subsequent modification of the patient's functional state has been shown. The results of ineffective influence, effective influence and successful influence with changes in the dynamics of the level of plasticity of neurodynamic activity of the brain are considered

scholarly journals Urate-induced epigenetic modifications in myeloid cells

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
M. Badii ◽  
O. I. Gaal ◽  
M. C. Cleophas ◽  
V. Klück ◽  
R. Davar ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Whole Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Myeloid Cells ◽  
Cytokine Signaling ◽  
Human Monocytes ◽  
Methylation Array ◽  
Histone 3 ◽  
Msu Crystals

Abstract Objectives Hyperuricemia is a metabolic condition central to gout pathogenesis. Urate exposure primes human monocytes towards a higher capacity to produce and release IL-1β. In this study, we assessed the epigenetic processes associated to urate-mediated hyper-responsiveness. Methods Freshly isolated human peripheral blood mononuclear cells or enriched monocytes were pre-treated with solubilized urate and stimulated with LPS with or without monosodium urate (MSU) crystals. Cytokine production was determined by ELISA. Histone epigenetic marks were assessed by sequencing immunoprecipitated chromatin. Mice were injected intraarticularly with MSU crystals and palmitate after inhibition of uricase and urate administration in the presence or absence of methylthioadenosine. DNA methylation was assessed by methylation array in whole blood of 76 participants with normouricemia or hyperuricemia. Results High concentrations of urate enhanced the inflammatory response in vitro in human cells and in vivo in mice, and broad-spectrum methylation inhibitors reversed this effect. Assessment of histone 3 lysine 4 trimethylation (H3K4me3) and histone 3 lysine 27 acetylation (H3K27ac) revealed differences in urate-primed monocytes compared to controls. Differentially methylated regions (e.g. HLA-G, IFITM3, PRKAB2) were found in people with hyperuricemia compared to normouricemia in genes relevant for inflammatory cytokine signaling. Conclusion Urate alters the epigenetic landscape in selected human monocytes or whole blood of people with hyperuricemia compared to normouricemia. Both histone modifications and DNA methylation show differences depending on urate exposure. Subject to replication and validation, epigenetic changes in myeloid cells may be a therapeutic target in gout.

Effect of Low-Intensity Microwave Radiation on Monoamine Neurotransmitters and Their Key Regulating Enzymes in Rat Brain

2015 ◽  
Vol 73 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Kanu Megha ◽  
Pravin S. Deshmukh ◽  
Alok K. Ravi ◽  
Ashok K. Tripathi ◽  
Mahesh P. Abegaonkar ◽  
...  
Keyword(s):  
Rat Brain ◽  
Microwave Radiation ◽  
Monoamine Neurotransmitters ◽  
Low Intensity
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