scholarly journals Adult mouse liver dissociation (on ice) v2 (protocols.io.yd2fs8e)

protocols.io ◽  
2019 ◽  
Author(s):  
Andrew Potter
Keyword(s):  
Mouse Liver ◽  
Adult Mouse ◽  
Adult Mouse Liver

AN IMPROVED METHOD FOR CHROMOSOME PREPARATION FROM MOUSE LIVER

1971 ◽  
Vol 13 (3) ◽  
pp. 612-617 ◽  
Author(s):  
N. C. Sun ◽  
E. H. Y. Chu
Keyword(s):  
Mitotic Activity ◽  
Subcutaneous Injection ◽  
Mouse Liver ◽  
Adult Mouse ◽  
Regenerating Liver ◽  
Improved Method ◽  
Mitotic Chromosomes ◽  
Simple Method ◽  
Cytogenetic Analyses ◽  
Adult Mouse Liver

A simple method for preparing mitotic chromosomes from adult mouse liver is described. The procedure involves the accumulation of metaphases in regenerating liver resulting from CCI4 treatment, followed by Trypsin perfusion, hypotonic pretreatment and fixation of cells, and flame-drying of slides to spread chromosomes. Approximately 2 × 106 intact liver cells can be obtained from a single mouse liver – enough to prepare 50 slides. A peak mitotic activity, with more than 1% of cells in mitosis, was observed 72 hr after subcutaneous injection of 0.1 ml of 45% CCI4 per animal. The distribution of diploid, tetraploid, and octaploid cells in mitosis was about 86, 11, and 3%, respectively. The abundant number of analyzable metaphases in such preparations makes this method valuable for cytogenetic analyses of normally non-proliferating tissue from adult laboratory mammals.

Functional liver tissue engineering by an adult mouse liver-derived neuro-glia antigen 2-expressing stem/progenitor population

2017 ◽  
Vol 12 (1) ◽  
pp. e190-e202 ◽  
Author(s):  
Hongyu Zhang ◽  
Christopher T. Siegel ◽  
Jing Li ◽  
Jiejuan Lai ◽  
Ling Shuai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Liver Tissue ◽  
Mouse Liver ◽  
Adult Mouse ◽  
Liver Tissue Engineering ◽  
Adult Mouse Liver

scholarly journals Direct Lineage Conversion of Adult Mouse Liver Cells and B Lymphocytes to Neural Stem Cells

2014 ◽  
Vol 3 (6) ◽  
pp. 948-956 ◽  
Author(s):  
John P. Cassady ◽  
Ana C. D’Alessio ◽  
Sovan Sarkar ◽  
Vardhan S. Dani ◽  
Zi Peng Fan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
B Lymphocytes ◽  
Mouse Liver ◽  
Adult Mouse ◽  
Liver Cells ◽  
Direct Lineage Conversion ◽  
Adult Mouse Liver

scholarly journals Disruption of the c/ebp alpha gene in adult mouse liver.

1997 ◽  
Vol 17 (10) ◽  
pp. 6014-6022 ◽  
Author(s):  
Y H Lee ◽  
B Sauer ◽  
P F Johnson ◽  
F J Gonzalez
Keyword(s):  
Mouse Liver ◽  
Adult Mouse ◽  
Glycogen Synthase ◽  
Factor Ix ◽  
Conditional Knockout ◽  
Hepatic Glycogen ◽  
Recombination System ◽  
Genes Encoding ◽  
Factor C ◽  
Adult Mouse Liver

The liver-enriched transcription factor C/EBP alpha has been implicated in the regulation of numerous liver-specific genes. It was previously reported that mice carrying a homozygous null mutation at the c/ebp alpha locus died as neonates due to the absence of hepatic glycogen and the resulting hypoglycemia. However, the lethal phenotype precluded further analysis of the role of C/EBP alpha in hepatic gene regulation in adult mice. To circumvent this problem, we constructed a conditional knockout allele of c/ebp alpha by using the Cre/loxP recombination system. Homozygous c/ebp-loxP mice, (c/ebp alpha(fl/fl);fl, flanked by loxP sites) were found to be indistinguishable from their wild-type counterparts. However, when Cre recombinase was delivered to hepatocytes of adult c/ebp alpha(fl/fl) mice by infusion of a recombinant adenovirus carrying the cre gene, more than 80% of the c/ebp alpha(fl/fl) genes were deleted specifically in liver and C/EBP alpha expression was reduced by 90%. This condition resulted in a reduced level of bilirubin UDP-glucuronosyltransferase expression in the liver. After several days, the knockout mice developed severe jaundice due to an increase in unconjugated serum bilirubin. The expression of genes encoding phosphoenolpyruvate carboxykinase, glycogen synthase, and factor IX was also strongly reduced in adult conditional-knockout animals, while the expression of transferrin, apolipoprotein B, and insulin-like growth factor I genes was not affected. These results establish C/EBP alpha as an essential transcriptional regulator of genes encoding enzymes involved in bilirubin detoxification and gluconeogenesis in adult mouse liver.

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