Functional liver tissue engineering by an adult mouse liver-derived neuro-glia antigen 2-expressing stem/progenitor population

2017 ◽  
Vol 12 (1) ◽  
pp. e190-e202 ◽  
Author(s):  
Hongyu Zhang ◽  
Christopher T. Siegel ◽  
Jing Li ◽  
Jiejuan Lai ◽  
Ling Shuai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Liver Tissue ◽  
Mouse Liver ◽  
Adult Mouse ◽  
Liver Tissue Engineering ◽  
Adult Mouse Liver

Liver tissue engineering via hyperbranched polypyrrole scaffolds

2019 ◽  
Vol 69 (17) ◽  
pp. 1112-1122 ◽  
Author(s):  
Maryam Hatamzadeh ◽  
Raana Sarvari ◽  
Bakhshali Massoumi ◽  
Samira Agbolaghi ◽  
Fatemeh Samadian
Keyword(s):  
Tissue Engineering ◽  
Liver Tissue ◽  
Liver Tissue Engineering

scholarly journals Graphene-based 3D scaffolds in tissue engineering: fabrication, applications, and future scope in liver tissue engineering

2019 ◽  
Vol Volume 14 ◽  
pp. 5753-5783 ◽  
Author(s):  
Renu Geetha Bai ◽  
Kasturi Muthoosamy ◽  
Sivakumar Manickam ◽  
Ali Hilal-Alnaqbi
Keyword(s):  
Tissue Engineering ◽  
Liver Tissue ◽  
3D Scaffolds ◽  
Liver Tissue Engineering

scholarly journals Hydrogels for Liver Tissue Engineering

2019 ◽  
Vol 6 (3) ◽  
pp. 59 ◽  
Author(s):  
Shicheng Ye ◽  
Jochem W.B. Boeter ◽  
Louis C. Penning ◽  
Bart Spee ◽  
Kerstin Schneeberger
Keyword(s):  
Tissue Engineering ◽  
Liver Tissue ◽  
Drug Testing ◽  
In Vitro Models ◽  
Liver Tissue Engineering ◽  
Synthetic Materials ◽  
Toxicological Studies ◽  
End Stage

Bioengineered livers are promising in vitro models for drug testing, toxicological studies, and as disease models, and might in the future be an alternative for donor organs to treat end-stage liver diseases. Liver tissue engineering (LTE) aims to construct liver models that are physiologically relevant. To make bioengineered livers, the two most important ingredients are hepatic cells and supportive materials such as hydrogels. In the past decades, dozens of hydrogels have been developed to act as supportive materials, and some have been used for in vitro models and formed functional liver constructs. However, currently none of the used hydrogels are suitable for in vivo transplantation. Here, the histology of the human liver and its relationship with LTE is introduced. After that, significant characteristics of hydrogels are described focusing on LTE. Then, both natural and synthetic materials utilized in hydrogels for LTE are reviewed individually. Finally, a conclusion is drawn on a comparison of the different hydrogels and their characteristics and ideal hydrogels are proposed to promote LTE.

AN IMPROVED METHOD FOR CHROMOSOME PREPARATION FROM MOUSE LIVER

1971 ◽  
Vol 13 (3) ◽  
pp. 612-617 ◽  
Author(s):  
N. C. Sun ◽  
E. H. Y. Chu
Keyword(s):  
Mitotic Activity ◽  
Subcutaneous Injection ◽  
Mouse Liver ◽  
Adult Mouse ◽  
Regenerating Liver ◽  
Improved Method ◽  
Mitotic Chromosomes ◽  
Simple Method ◽  
Cytogenetic Analyses ◽  
Adult Mouse Liver

A simple method for preparing mitotic chromosomes from adult mouse liver is described. The procedure involves the accumulation of metaphases in regenerating liver resulting from CCI4 treatment, followed by Trypsin perfusion, hypotonic pretreatment and fixation of cells, and flame-drying of slides to spread chromosomes. Approximately 2 × 106 intact liver cells can be obtained from a single mouse liver – enough to prepare 50 slides. A peak mitotic activity, with more than 1% of cells in mitosis, was observed 72 hr after subcutaneous injection of 0.1 ml of 45% CCI4 per animal. The distribution of diploid, tetraploid, and octaploid cells in mitosis was about 86, 11, and 3%, respectively. The abundant number of analyzable metaphases in such preparations makes this method valuable for cytogenetic analyses of normally non-proliferating tissue from adult laboratory mammals.

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