Procedures for labeling of bacteria with PKH kit (Fluorescent Cell Linker Kit for General Membrane Labeling – Sigma-Aldrich) v1 (protocols.io.phcdj2w)

protocols.io ◽  
2018 ◽  
Author(s):  
Sabrina Alves ◽  
Chyntia D ◽  
Maria Cristina
Keyword(s):  
Fluorescent Cell ◽  
Membrane Labeling

DebarcodeR Increases Fluorescent Cell Barcoding Capacity and Accuracy

2021 ◽  
Author(s):  
Benjamin J. Reisman ◽  
Sierra M. Barone ◽  
Brian O. Bachmann ◽  
Jonathan M. Irish
Keyword(s):  
Fluorescent Cell

I2 catalyzed access of spiro[indoline-3,4′-pyridine] appended amine dyad: new ON–OFF chemosensors for Cu2+ and imaging in living cells

2018 ◽  
Vol 16 (2) ◽  
pp. 302-315 ◽  
Author(s):  
Animesh Mondal ◽  
Barnali Naskar ◽  
Sanchita Goswami ◽  
Chandraday Prodhan ◽  
Keya Chaudhuri ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hepg2 Cells ◽  
Cell Imaging ◽  
Colorimetric Detection ◽  
Living Cells ◽  
Fluorescent Cell

An efficient, easily tuneable route to construct a structurally diverse organic fluorescent probe and its applications towards the colorimetric detection of Cu2+ ions and in vitro fluorescent cell imaging of Cu2+ in HepG2 cells.

scholarly journals Data-driven modeling reveals cell behaviors controlling self-organization during Myxococcus xanthus development

2017 ◽  
Vol 114 (23) ◽  
pp. E4592-E4601 ◽  
Author(s):  
Christopher R. Cotter ◽  
Heinz-Bernd Schüttler ◽  
Oleg A. Igoshin ◽  
Lawrence J. Shimkets
Keyword(s):  
Cell Tracking ◽  
Myxococcus Xanthus ◽  
Cell Movement ◽  
Modeling Method ◽  
Data Driven ◽  
Self Organization ◽  
Emergent Properties ◽  
Developmental Cycle ◽  
Fluorescent Cell ◽  
Cell Behaviors

Collective cell movement is critical to the emergent properties of many multicellular systems, including microbial self-organization in biofilms, embryogenesis, wound healing, and cancer metastasis. However, even the best-studied systems lack a complete picture of how diverse physical and chemical cues act upon individual cells to ensure coordinated multicellular behavior. Known for its social developmental cycle, the bacterium Myxococcus xanthus uses coordinated movement to generate three-dimensional aggregates called fruiting bodies. Despite extensive progress in identifying genes controlling fruiting body development, cell behaviors and cell–cell communication mechanisms that mediate aggregation are largely unknown. We developed an approach to examine emergent behaviors that couples fluorescent cell tracking with data-driven models. A unique feature of this approach is the ability to identify cell behaviors affecting the observed aggregation dynamics without full knowledge of the underlying biological mechanisms. The fluorescent cell tracking revealed large deviations in the behavior of individual cells. Our modeling method indicated that decreased cell motility inside the aggregates, a biased walk toward aggregate centroids, and alignment among neighboring cells in a radial direction to the nearest aggregate are behaviors that enhance aggregation dynamics. Our modeling method also revealed that aggregation is generally robust to perturbations in these behaviors and identified possible compensatory mechanisms. The resulting approach of directly combining behavior quantification with data-driven simulations can be applied to more complex systems of collective cell movement without prior knowledge of the cellular machinery and behavioral cues.

Differential Basement Membrane Labeling in Epidermal Tumors

1990 ◽  
Vol 12 (3) ◽  
pp. 311
Author(s):  
Margaret L. Price ◽  
N. Kirkham ◽  
B. Gibson ◽  
Irene M. Leigh
Keyword(s):  
Basement Membrane ◽  
Membrane Labeling

scholarly journals Fluorescent Cell Staining Methods for Living Hypsibius exemplaris Embryos

2018 ◽  
Vol 2018 (11) ◽  
pp. pdb.prot106021 ◽  
Author(s):  
Kristen M. McGreevy ◽  
Kira L. Heikes ◽  
Shiri Kult ◽  
Marla E. Tharp ◽  
Bob Goldstein
Keyword(s):  
Fluorescent Cell ◽  
Cell Staining ◽  
Staining Methods

scholarly journals Elevated Mating Frequency in Honey Bee (Hymenoptera: Apidae) Queens Exposed to the Miticide Amitraz During Development

2020 ◽  
Author(s):  
Elizabeth M Walsh ◽  
Mark A Janowiecki ◽  
Kyle Zhu ◽  
Nancy H Ing ◽  
Edward Lind Vargo ◽  
...  
Keyword(s):  
Reproductive Health ◽  
Honey Bee ◽  
The United States ◽  
Mating Frequency ◽  
Sperm Viability ◽  
Fluorescent Cell ◽  
Queen Rearing ◽  
Polymorphic Microsatellite ◽  
Cell Counter ◽  
Practical Implications

Abstract Most honey bee (Apis mellifera Linnaeus, 1758) (Hymenoptera: Apidae) colonies in the United States have been exposed to the beekeeper-applied miticides amitraz, coumaphos, and tau-fluvalinate. Colonies are also often exposed to agrochemicals, which bees encounter on foraging trips. These and other lipophilic pesticides bind to the beeswax matrix of comb, exposing developing bees. We explored whether queen-rearing beeswax containing pesticides affects the reproductive health of mated queens. We predicted that queens reared in pesticide-free beeswax would have higher mating frequencies and sperm viability of stored sperm compared with queens reared in wax containing pesticides. Mating frequency and sperm viability are two traditional measurements associated with queen reproductive health. To test these hypotheses, we reared queens in beeswax-coated cups that were pesticide free or contained field-relevant concentrations of 1) amitraz, 2) a combination of tau-fluvalinate and coumaphos, or 3) a combination of the agrochemicals chlorothalonil and chlorpyrifos. We then collected queens once they mated to determine sperm viability, using a dual fluorescent cell counter, and mating frequency, genotyping immature worker offspring at eight polymorphic microsatellite loci. Sperm viability did not differ between control queens and those reared in pesticide-laden wax. However, queens exposed to amitraz during development exhibited higher mating frequency than queens reared in pesticide-free beeswax or beeswax containing the other pesticide combinations. Our results suggest that miticide exposure during development affects queen mating frequency but not sperm viability, at least in newly mated queens. This finding, which has practical implications for commercial queen rearing and overall colony health, calls for further study.

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