THP1 cell line differentiation v1

protocols.io ◽  
2016 ◽  
Author(s):  
Rabia Khan
Keyword(s):  
Cell Line ◽  
Thp1 Cell

scholarly journals Arsenic trioxide induces apoptosis in the THP1 cell line by downregulating EVI-1

2014 ◽  
Vol 8 (1) ◽  
pp. 85-90 ◽  
Author(s):  
LING-YUN ZHOU ◽  
FANG-YUAN CHEN ◽  
LI-JING SHEN ◽  
HAI-XIA WAN ◽  
JI-HUA ZHONG
Keyword(s):  
Cell Line ◽  
Arsenic Trioxide ◽  
Thp1 Cell

An Evaluation of the Cytotoxic Effect of the Natural Product Aaptamine Against t(4;11) Leukemias

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 1624-1624 ◽  
Author(s):  
Chandrima Sinha ◽  
John Bowling ◽  
Aman Seth ◽  
Bensheng Ju ◽  
Bhaskar Kahali ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
Cell Lines ◽  
Leukemia Cell ◽  
Treated Group ◽  
Jurkat Cells ◽  
G1 Arrest ◽  
Thp1 Cell ◽  
Rb Phosphorylation

Abstract The marine environment has been shown to be a rich source of pharmacologically-active secondary metabolites. Three marine- compounds have FDA approval for cancer indications. Aaptamine is a sponge-derived alkaloid that exhibits multiple pharmacological activities including proapoptotic/antiproliferative effects on leukemia cell lines. The effect of the aaptamine class has not been previously studied for high risk leukemias with mixed-lineage leukemia (MLL) gene rearrangements. Using the CellTiter-Glo cell viability assay we evaluated the cytotoxic effect of aaptamine against a panel of leukemia cell lines. We observed that cell lines containing t(4;11) are the most sensitive to aaptamine. Translocation (4;11) is associated with mixed-lineage leukemia and responsible for a very aggressive and refractory pediatric leukemia. Specifically, infants less than one year with t(4;11) have poor survival rates (≈ 19%) and new therapies are urgently needed. Interestingly other MLL cell lines that contain t(9;11) are comparatively less susceptible to aaptamine-mediated cytotoxicity. Jurkat cells overexpressing MLL-AF4 fusion protein are also more sensitive to aaptamine-induced cytotoxicity than wild type or MLL-AF9 overexpressing Jurkat cells indicating the specificity of aaptamine for t(4;11). To further confirm the specificity we conducted a flow based apoptosis assay and observed that aaptamine induces significant apoptosis and necrosis in RS4;11 and MV4;11 cell lines starting at 10µM but not in the t(9;11) containing THP1 cell line. We also found that aaptamine treatment induced G0/G1 arrest specifically in t(4;11) containing cell lines but not in THP1. Additionally we observed that aaptamine did not induce any resistance to the sensitive cell lines after 27 days of chronic exposure. Importantly the compound was well tolerated by healthy activated PBMCs and mice at high concentrations. In order to decipher the mechanism of specificity, we conducted a global proteomic study with treated and untreated RS4;11 and THP1 cell lines. Our proteomic data revealed a significant upregulation of p21 and p27 in aaptamine treated RS4;11 cells but not in THP1. In agreement with the proteomic data, we observed a dose-dependent upregulation of p21 and p27 in both protein and mRNA levels in RS4;11 and MV4;11 cells but not in resistant THP1 cells. Using p21 and p27 promoter-driven luciferase reporter constructs, we observed a significant upregulation of luminescence signal in the RS4;11 cell line at much lower concentration of aaptamine (1µM) whereas the THP1 cell line required 50µM of aaptamine for significant increase in luminescence signal. Cyclin-dependent kinase regulates the G1/S cell cycle transition by phosphorylating retinoblastoma protein (RB). Upregulation of cyclin-dependent kinase inhibitors, such as p27 and p21, promote RB hypophosphorylation and induce G0/G1 arrest. To confirm that this molecular mechanism is responsible for aaptamine induced G0/G1 arrest, we investigated the effect of aaptamine on Rb phosphorylation. We observed a dose dependent downregulation of Rb phosphorylation by aaptamine in sensitive cell lines and predicted it as a major cause of cell cycle arrest. Previous studies have shown that translocation (4;11) is associated with p27 upregulation; thus we hypothesize by further upregulating p27, aaptamine may induce G0/G1 arrest specifically in t(4;11) containing cell lines. To validate the efficacy of aaptamine in vivo, we xenografted 10 NSG mice with 1 million luciferase expressing RS4;11 cells. Four days after leukemia induction we treated half of the mice with subcutaneous injection of aaptamine (100mg/kg, daily) and the other half received vehicle treatment. Bioluminescence imaging (BLI) data revealed a significantly lower disease (p< 0.03) burden in the aaptamine treated group compared to vehicle treated group after 2 weeks. These findings are being confirmed in patient samples. Additional aaptamine analogs are being designed and will be evaluated for improved therapeutic efficacy. Together our in vitro and in vivo findings suggest that by inducing p21 and p27 aaptamine can induce cell cycle arrest and eventually apoptosis specifically in leukemia cells that contain t(4;11) with relatively low toxicity . Therefore the aaptamine class of drug may provide additional therapeutic options for t(4;11) containing high-risk MLL leukemia patients. Disclosures No relevant conflicts of interest to declare.

scholarly journals Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay

2009 ◽  
Vol 17 (5) ◽  
pp. 457-461 ◽  
Author(s):  
Gabriela Tavares Bekner Correa ◽  
Gabriel Alves Costa Veranio ◽  
Licínio Esmaeraldo Silva ◽  
Raphael Hirata Junior ◽  
Jeffry M. Coil ◽  
...  
Keyword(s):  
Cell Line ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Trypan Blue ◽  
Thp1 Cell ◽  
Root Canal Sealers

scholarly journals System biology analysis of THP1 cell line as in vitro model to evaluate yellow fever vaccine

2021 ◽  
Author(s):  
Andréa Silva ◽  
Tamiris Barros ◽  
Thyago Calvo ◽  
Juliana Melgaço ◽  
Luciana Tubarão ◽  
...  
Keyword(s):  
Cell Line ◽  
Yellow Fever ◽  
System Biology ◽  
In Vitro Model ◽  
Yellow Fever Vaccine ◽  
Thp1 Cell ◽  
Vitro Model

scholarly journals Identification of a persisting bacterial contamination of cell culture as Brevibacterium

2020 ◽  
Author(s):  
P.P Mahesh ◽  
R. J Retnakumar ◽  
Sathish Mundayoor
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Cell Lines ◽  
Bacterial Contamination ◽  
Culture Medium ◽  
Life Sciences ◽  
Thp1 Cell ◽  
Healthy Cell ◽  
Important Prerequisite

AbstractCell culture is an important prerequisite for many of the basic and applied researches in life sciences and contamination is a major problem faced by the researchers in maintaining healthy cell lines. We noted a dot like contamination in our THP1 cell line, which does not make the culture medium turbid as fast as other common contaminants and remains in the culture for many days before it makes the culture unusable. The contaminant was identified as a member of the genus Brevibacterium and we could find that the antibiotic rifampicin effectively suppresses the growth of the bacterium.

Genome scale metabolic model driven strategy to delineate host response to Mycobacterium tuberculosis infection

2021 ◽  
Author(s):  
Ankur Gupta ◽  
Ajay Kumar ◽  
Rajat Anand ◽  
Nandadulal Bairagi ◽  
Samrat Chatterjee
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cell Line ◽  
High Throughput ◽  
Host Response ◽  
Metabolic Model ◽  
Thp1 Cell ◽  
Proteomics Data ◽  
Mycobacterium Tuberculosis Infection ◽  
Model Driven ◽  
Genome Scale

We analyzed high throughput proteomics data reflecting the response of the Mϕ-like THP1 cell line to Mycobacterium tuberculosis (M. tuberculosis) infection.

Ultrastructure of NPC/HK1 cells heterotransplanted in athymic nude mice

1982 ◽  
Vol 40 ◽  
pp. 236-237
Author(s):  
E.C. Chew ◽  
C.L. Li ◽  
D.P. Huang ◽  
H.C. Ho ◽  
L.S. Mak ◽  
...  
Keyword(s):  
Cell Line ◽  
Nude Mice ◽  
Biopsy Specimen ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Recurrent Tumour ◽  
Athymic Nude Mice ◽  
Cell Line Establishment ◽  
Fine Structures ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been established from a biopsy specimen of a recurrent tumour of the nasopharynx which was histologically diagnosed as a moderately to well differentiated squamous cell carcinoma. A definite decrease in the amount of tonofilaments and desmosomes in the NPC/HK1 cells during the cell line establishment was observed. The present communication reports on the fine structures of the NPC/HK1 cells heterotraneplanted in athymic nude mice.

Ultrastructure of Choriocarcinoma in Vitro

1969 ◽  
Vol 27 ◽  
pp. 362-363
Author(s):  
John C. Garancis ◽  
R. A. Pattillo
Keyword(s):  
Cell Line ◽  
Physiological Function ◽  
Cell System ◽  
Bewo Cell ◽  
Bewo Cells ◽  
Gestational Choriocarcinoma ◽  
Bewo Cell Line

Growth of cell system (BeWo-cell line) derived from human gestational choriocarcinoma has been established and continuously maintained in-vitro. Furthermore, it is evident from the previous studies that this cell line has retained the physiological function of the placental trophoblasts, namely the synthesis of human chorionic gonadotrophil(HCG).The BeWo cells were relatively small and possessed single nuclei, thus indicating that this cell line consists exclusively of cytotrophoblasts. In some instances cells appeared widely separated and their lateral surfaces were provided with numerous microvilli (Fig.1).

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