scholarly journals Action Spectra protocol for Opentrons OT-1 liquid handling robot  v1 (protocols.io.br6vm9e6)

protocols.io ◽  
2021 ◽  
Author(s):  
andrei.herdean not provided
Keyword(s):  
Action Spectra ◽  
Liquid Handling ◽  
Liquid Handling Robot

scholarly journals PlasmoTron: an open-source platform for automated culture of malaria parasites

2017 ◽  
Author(s):  
Theo Sanderson ◽  
Julian C. Rayner
Keyword(s):  
Open Source ◽  
Genetic Modification ◽  
Malaria Parasite ◽  
Scale Up ◽  
Malaria Parasites ◽  
Parasite Culture ◽  
Liquid Handling ◽  
Liquid Handling Robot

We have created a system which allows an inexpensive opensource liquid-handling robot to automate most aspects of bloodstage malaria parasite culture. Parasites are cultured in multiwell microplates, with their details recorded in a database. Information in the database is used to generate commands for the robot to feed, monitor and passage parasite cultures. We show that the system is capable of raising cultures after transfection and then maintaining them at desired parasitaemias, facilitiating significant scale up of both routine culture and experimental genetic modification. The PlasmoTron software is available at plasmotron.org.

UI Design for an Engineering Process: Programming Experiments on a Liquid Handling Robot

2017 ◽  
Author(s):  
Farzad Nejatimoharrami ◽  
Andres Faina ◽  
Andrea Jovanovic ◽  
Olivier St-Cyr ◽  
Mark Chignell ◽  
...  
Keyword(s):  
Engineering Process ◽  
Ui Design ◽  
Liquid Handling ◽  
Liquid Handling Robot

scholarly journals High-Throughput Molecular Testing of Ticks Using a Liquid-Handling Robot

2005 ◽  
Vol 42 (6) ◽  
pp. 1063-1067 ◽  
Author(s):  
John R. Moriarity ◽  
Amanda D. Loftis ◽  
Gregory A. Dasch
Keyword(s):  
High Throughput ◽  
Molecular Testing ◽  
Liquid Handling ◽  
Liquid Handling Robot

scholarly journals Automation of column-based radiochemical separations: a comparison of fluidic, robotic, and hybrid architectures

2011 ◽  
Vol 1 (1) ◽  
pp. 13-19 ◽  
Author(s):  
J. W. Grate ◽  
M. J. O'Hara ◽  
A. F. Farawila ◽  
R. M. Ozanich ◽  
S. L. Owsley
Keyword(s):  
Anion Exchange ◽  
Radiochemical Separation ◽  
General Purpose ◽  
Automated Systems ◽  
New Approach ◽  
Radiochemical Separations ◽  
Liquid Handling ◽  
Fraction Collector ◽  
Automated Processing ◽  
Liquid Handling Robot

AbstractTwo automated systems have been developed to perform column-based radiochemical separation procedures. These new systems are compared with past fluidic column separation architectures, with emphasis on using disposable components so that no sample contacts any surface that any other sample has contacted, and setting up samples and columns in parallel for subsequent automated processing. In the first new approach, a general purpose liquid handling robot has been modified and programmed to perform anion exchange separations using 2 mL bed columns in 6 mL plastic disposable column bodies. In the second new approach, a fluidic system has been developed to deliver clean reagents through disposable manual valves to six disposable columns, with a mechanized fraction collector that positions one of four rows of six vials below the columns. The samples are delivered to each column via a manual 3-port disposable valve from disposable syringes. This second approach, a hybrid of fluidic and mechanized components, is a simpler more efficient approach for performing anion exchange procedures for the recovery and purification of plutonium from samples. The automation architectures described can also be adapted to column-based extraction chromatography separations.

scholarly journals Semi-automated high-throughput substrate screening assay for nucleoside kinases

2021 ◽  
Author(s):  
Katja Hellendahl ◽  
Maryke Fehlau ◽  
Sebastian Hans ◽  
Peter Neubauer ◽  
Anke Kurreck
Keyword(s):  
High Throughput ◽  
Viral Infections ◽  
Screening Assay ◽  
Liquid Handling ◽  
Wide Range ◽  
High Throughput Assay ◽  
Liquid Handling Robot

Nucleoside kinases (NKs) are key enzymes involved in the in vivo phosphorylation of nucleoside analogues used as drugs to treat cancer or viral infections. Having different specificities, the characterization of NKs is essential for drug design and the production of nucleotide analogues in an in vitro enzymatic process. Therefore, a fast and reliable substrate screening assay for NKs is of great importance. Here, we report the validation of a well-known luciferase-based assay for the detection of NK activity in 96-well plate format. The assay was semi-automated using a liquid handling robot. A good linearity was demonstrated (r² >0.98) in the range of 0 to 500 µM ATP, and it was shown that also alternative phosphate donors like dATP or CTP were accepted by the luciferase. The developed high-throughput assay revealed comparable results to HPLC analysis. The assay was exemplary used for the comparison of the substrate spectra of four nucleoside kinases using 20 (8 natural and 12 modified) substrates. The screening results correlated well with literature data and, additionally, previously unknown substrates were identified for three of the NKs studied. Our results demonstrate that the developed semi-automated high-throughput assay is suitable to identify best performing NKs for a wide range of substrates.

scholarly journals Open-source personal pipetting robots with live-cell incubation and microscopy compatibility

2021 ◽  
Author(s):  
Philip Dettinger ◽  
Tobias Kull ◽  
Geethika Arekatla ◽  
Nouraiz Ahmed ◽  
Yang Zhang ◽  
...  
Keyword(s):  
Open Source ◽  
Time Lapse ◽  
Small Scale ◽  
Liquid Handling ◽  
Academic Settings ◽  
Stem And Progenitor Cells ◽  
Cell Incubation ◽  
Self Production ◽  
Liquid Handling Robot ◽  
Laboratory Member

Liquid handling robots have the potential to automate many procedures in life sciences. However, they are not in widespread use in academic settings, where funding, space and maintenance specialists are usually limiting. In addition, current robots require lengthy programming by specialists and are incompatible with most academic laboratories with constantly changing small-scale projects. Here, we present the Pipetting Helper Imaging Lid (PHIL), an inexpensive, small, open-source personal liquid handling robot. It is designed for inexperienced users, with self-production from cheap commercial and 3D-printable components and custom control software. PHIL successfully automated pipetting for e.g. tissue immunostainings and stimulations of live stem and progenitor cells during time-lapse microscopy. PHIL is cheap enough for any laboratory member to have their own personal pipetting robot(s), and enables users without programming skills to easily automate a large range of experiments.

scholarly journals A Liquid Handling Robot for Robust and Reproducible Preparation of Standard and Quality Control Samples in Bioanalysis

2017 ◽  
Vol 06 (01) ◽  
Author(s):  
Michael Groschl ◽  
Artur Markus ◽  
Simone Leyers ◽  
Rebeca Schibli ◽  
Sabine Zelger ◽  
...  
Keyword(s):  
Quality Control ◽  
Liquid Handling ◽  
Liquid Handling Robot ◽  
Control Samples
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