scholarly journals Automation of column-based radiochemical separations: a comparison of fluidic, robotic, and hybrid architectures

2011 ◽  
Vol 1 (1) ◽  
pp. 13-19 ◽  
Author(s):  
J. W. Grate ◽  
M. J. O'Hara ◽  
A. F. Farawila ◽  
R. M. Ozanich ◽  
S. L. Owsley
Keyword(s):  
Anion Exchange ◽  
Radiochemical Separation ◽  
General Purpose ◽  
Automated Systems ◽  
New Approach ◽  
Radiochemical Separations ◽  
Liquid Handling ◽  
Fraction Collector ◽  
Automated Processing ◽  
Liquid Handling Robot

AbstractTwo automated systems have been developed to perform column-based radiochemical separation procedures. These new systems are compared with past fluidic column separation architectures, with emphasis on using disposable components so that no sample contacts any surface that any other sample has contacted, and setting up samples and columns in parallel for subsequent automated processing. In the first new approach, a general purpose liquid handling robot has been modified and programmed to perform anion exchange separations using 2 mL bed columns in 6 mL plastic disposable column bodies. In the second new approach, a fluidic system has been developed to deliver clean reagents through disposable manual valves to six disposable columns, with a mechanized fraction collector that positions one of four rows of six vials below the columns. The samples are delivered to each column via a manual 3-port disposable valve from disposable syringes. This second approach, a hybrid of fluidic and mechanized components, is a simpler more efficient approach for performing anion exchange procedures for the recovery and purification of plutonium from samples. The automation architectures described can also be adapted to column-based extraction chromatography separations.

scholarly journals PlasmoTron: an open-source platform for automated culture of malaria parasites

2017 ◽  
Author(s):  
Theo Sanderson ◽  
Julian C. Rayner
Keyword(s):  
Open Source ◽  
Genetic Modification ◽  
Malaria Parasite ◽  
Scale Up ◽  
Malaria Parasites ◽  
Parasite Culture ◽  
Liquid Handling ◽  
Liquid Handling Robot

We have created a system which allows an inexpensive opensource liquid-handling robot to automate most aspects of bloodstage malaria parasite culture. Parasites are cultured in multiwell microplates, with their details recorded in a database. Information in the database is used to generate commands for the robot to feed, monitor and passage parasite cultures. We show that the system is capable of raising cultures after transfection and then maintaining them at desired parasitaemias, facilitiating significant scale up of both routine culture and experimental genetic modification. The PlasmoTron software is available at plasmotron.org.

ACO powered by Local Searches Algorithms for the Solution of TSP Problems

2020 ◽  
Vol 1 (1) ◽  
pp. 1-10
Author(s):  
Nisreen L. Ahmed
Keyword(s):  
Ant Colony Optimization ◽  
Optimization Technique ◽  
General Purpose ◽  
Ant Colony ◽  
Ant Colony Optimization Algorithm ◽  
New Approach ◽  
Local Searches ◽  
Probabilistic Technique ◽  
The Social ◽  
Great Deluge Algorithm

Swarm intelligence is a relatively new approach to problem solving that takes inspiration from the social behaviors of insects and other animals. Ants, in particular, have inspired a number of methods and techniques among which the most studied and successful is the general-purpose optimization technique, also known as ant colony optimization, In computer science and operations research, the ant colony optimization algorithm (ACO) is a probabilistic technique for solving computational problems which can be reduced to finding good paths through graphs.  Ant Colony Optimization (ACO) algorithm is used to arrive at the best solution for TSP. In this article, the researcher has introduced ways to use a great deluge algorithm with the ACO algorithm to increase the ability of the ACO in finding the best tour (optimal tour). Results are given for different TSP problems by using ACO with great deluge and other local search algorithms.

scholarly journals Partial Compilation of ASP Programs

2019 ◽  
Vol 19 (5-6) ◽  
pp. 857-873 ◽  
Author(s):  
BERNARDO CUTERI ◽  
CARMINE DODARO ◽  
FRANCESCO RICCA ◽  
PETER SCHÜLLER
Keyword(s):  
Experimental Analysis ◽  
Ad Hoc ◽  
Blow Up ◽  
State Of The Art ◽  
Answer Set Programming ◽  
General Purpose ◽  
Combinatorial Problems ◽  
New Approach ◽  
Input Program ◽  
Evaluation Algorithm

AbstractAnswer Set Programming (ASP) is a well-known declarative formalism in logic programming. Efficient implementations made it possible to apply ASP in many scenarios, ranging from deductive databases applications to the solution of hard combinatorial problems. State-of-the-art ASP systems are based on the traditional ground&solve approach and are general-purpose implementations, i.e., they are essentially built once for any kind of input program. In this paper, we propose an extended architecture for ASP systems, in which parts of the input program are compiled into an ad-hoc evaluation algorithm (i.e., we obtain a specific binary for a given program), and might not be subject to the grounding step. To this end, we identify a condition that allows the compilation of a sub-program, and present the related partial compilation technique. Importantly, we have implemented the new approach on top of a well-known ASP solver and conducted an experimental analysis on publicly-available benchmarks. Results show that our compilation-based approach improves on the state of the art in various scenarios, including cases in which the input program is stratified or the grounding blow-up makes the evaluation unpractical with traditional ASP systems.

UI Design for an Engineering Process: Programming Experiments on a Liquid Handling Robot

2017 ◽  
Author(s):  
Farzad Nejatimoharrami ◽  
Andres Faina ◽  
Andrea Jovanovic ◽  
Olivier St-Cyr ◽  
Mark Chignell ◽  
...  
Keyword(s):  
Engineering Process ◽  
Ui Design ◽  
Liquid Handling ◽  
Liquid Handling Robot

scholarly journals High-Throughput Molecular Testing of Ticks Using a Liquid-Handling Robot

2005 ◽  
Vol 42 (6) ◽  
pp. 1063-1067 ◽  
Author(s):  
John R. Moriarity ◽  
Amanda D. Loftis ◽  
Gregory A. Dasch
Keyword(s):  
High Throughput ◽  
Molecular Testing ◽  
Liquid Handling ◽  
Liquid Handling Robot

scholarly journals Semi-automated high-throughput substrate screening assay for nucleoside kinases

2021 ◽  
Author(s):  
Katja Hellendahl ◽  
Maryke Fehlau ◽  
Sebastian Hans ◽  
Peter Neubauer ◽  
Anke Kurreck
Keyword(s):  
High Throughput ◽  
Viral Infections ◽  
Screening Assay ◽  
Liquid Handling ◽  
Wide Range ◽  
High Throughput Assay ◽  
Liquid Handling Robot

Nucleoside kinases (NKs) are key enzymes involved in the in vivo phosphorylation of nucleoside analogues used as drugs to treat cancer or viral infections. Having different specificities, the characterization of NKs is essential for drug design and the production of nucleotide analogues in an in vitro enzymatic process. Therefore, a fast and reliable substrate screening assay for NKs is of great importance. Here, we report the validation of a well-known luciferase-based assay for the detection of NK activity in 96-well plate format. The assay was semi-automated using a liquid handling robot. A good linearity was demonstrated (r² >0.98) in the range of 0 to 500 µM ATP, and it was shown that also alternative phosphate donors like dATP or CTP were accepted by the luciferase. The developed high-throughput assay revealed comparable results to HPLC analysis. The assay was exemplary used for the comparison of the substrate spectra of four nucleoside kinases using 20 (8 natural and 12 modified) substrates. The screening results correlated well with literature data and, additionally, previously unknown substrates were identified for three of the NKs studied. Our results demonstrate that the developed semi-automated high-throughput assay is suitable to identify best performing NKs for a wide range of substrates.

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