Bacterial Transformation - Mix & Go Competent Cells - CHEM 584 v1 (protocols.io.bj2tkqen)

protocols.io ◽  
2020 ◽  
Author(s):  
Ken Christensen
Keyword(s):  
Bacterial Transformation ◽  
Competent Cells

Trypanosoma cruzi calreticulin: In vitro modulation of key immunogenic markers of both canine tumors and relevant immune competent cells

Immunobiology ◽  
2020 ◽  
Vol 225 (2) ◽  
pp. 151892 ◽  
Author(s):  
P. Cruz ◽  
E. Sosoniuk-Roche ◽  
I. Maldonado ◽  
C.G. Torres ◽  
A. Ferreira
Keyword(s):  
Trypanosoma Cruzi ◽  
Competent Cells ◽  
Canine Tumors

scholarly journals Aerobic bacterial transformation and biodegradation of dioxins: a review

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Salametu Saibu ◽  
Sunday A. Adebusoye ◽  
Ganiyu O. Oyetibo
Keyword(s):  
Bacterial Transformation

scholarly journals A mechanism for bacterial transformation of dimethylsulfide to dimethylsulfoxide: a missing link in the marine organic sulfur cycle

2016 ◽  
Vol 18 (8) ◽  
pp. 2754-2766 ◽  
Author(s):  
Ian Lidbury ◽  
Eileen Kröber ◽  
Zhidong Zhang ◽  
Yijun Zhu ◽  
J. Colin Murrell ◽  
...  
Keyword(s):  
Sulfur Cycle ◽  
Organic Sulfur ◽  
Bacterial Transformation ◽  
Missing Link

"Steady-state" competence for bacterial transformation

1968 ◽  
Vol 14 (3) ◽  
pp. 290-292
Author(s):  
Herbert H. Eichhorn ◽  
Shigeyo Arikawa ◽  
Stephen Zamenhof
Keyword(s):  
Bacillus Subtilis ◽  
Steady State ◽  
Continuous Culture ◽  
Generation Time ◽  
Bacterial Transformation ◽  
Short Period ◽  
Minimal Media ◽  
Harmful Effects ◽  
Specific Phase

The cells of Bacillus subtilis, grown in minimal media, are known to become competent for transformation for a short period at a specific phase of ceil growth. In the present work the cells (strain 168 ind−) were grown in continuous culture (chemostat; glucose limiting, generation time 4 h, 37 °C). Aliquots were removed at 20- to 24-h intervals and immediately tested for competence. The viability (41 h) was 97%. The initial very low competence increased up to 200-fold within the 24 h and remained at this high, slowly decreasing level for at least 168 h. It is concluded that a long-lasting competence may develop and persist in the cells in continuous culture ("steady-state"), without demonstrable harmful effects to the population.

scholarly journals Therapeutic Depletion of Axotomy Competent Cells in Amyotrophic Lateral Sclerosis (ALS)

2019 ◽  
Vol 2 (1) ◽  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Protein Networks ◽  
Large Protein ◽  
Anatomical Structures ◽  
Therapy Options ◽  
New Therapy ◽  
Competent Cells ◽  
Health And Disease ◽  
Insight Into ◽  
Lateral Sclerosis

The present paper addresses anatomically resolved protein networks by using the Imaging Cycler Microscopy (ICM/TIS) [1,2]. ICM is capable of resolving protein networks in intact anatomical structures at a power of combinatorial molecular resolution of 65,553k , where k is the number of co-mapped proteins, e.g. 100 proteins [1-4]. This method provides insight into the laws of the spatial communication of large protein networks in health and disease, which is essential for new therapy options in diseases.

scholarly journals Effect of Gold Nanoparticles Size Capped with Surfactant on the Transformation of Plasmid into Escherichia coli Bacteria

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Amr D ◽  
◽  
Attia N ◽  
Seufi A ◽  
Galal A ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticles ◽  
Heat Shock ◽  
Transformation Efficiency ◽  
Transformation Process ◽  
Bacterial Cells ◽  
Bacterial Transformation ◽  
Foreign Genes ◽  
Escherichia Coli Bacteria ◽  
Convenient Technique

Bacterial transformation has great importance in molecular biology, as it is used for introduction of foreign genes into bacterial cells either chemical or physical ways. Using calcium chloride to prepare competent cells and heat shock is the most widely used method for bacterial transformation. This method is an efficient and convenient technique but it has in some extent low transformation efficiency. Here we report the use of nanoparticles that significantly improve the transformation efficiency up to 10 times higher than the standard heat shock method by the assistance of (˜ 15, 25 nm) SDS capped gold nanoparticles in the transformation process that leads to the formation of temporary nano-channels across the bacterial cell wall to deliver plasmids into cells. Transformation of bacteria with plasmid was examined using Β-galactosidase assay.

scholarly journals Bacterial degradation of a mixture of hydroxy- and metoxypolychlorinated biphenyls

2019 ◽  
Vol 486 (3) ◽  
pp. 307-311
Author(s):  
D. O. Egorova ◽  
T. I. Gorbunova ◽  
M. G. Pervova ◽  
K. A. Plotnikova ◽  
T. D. Kiryanova ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls ◽  
Total Content ◽  
Bacterial Degradation ◽  
Specific Rate ◽  
Bacterial Transformation ◽  
Initial Concentration ◽  
Chemical Functionalization ◽  
Pcb Congeners ◽  
Total Absence ◽  
Rhodococcus Wratislaviensis

In this work, the possibility of combining the methods of chemical functionalization of a mixture of polychlorinated biphenyls before the formation of a mixture of hydroxy- and methoxy-chlorinated biphenyls and the bacterial transformation of the compounds obtained with the aim of their utilization was investigated. As a result of a 100% conversion, a mixture of compounds identified as methoxy- (30 compounds), hydroxy- (44 compounds) and methoxy (hydroxy) derivatives (47 compounds) was obtained by reacting a mixture of Sovol with MeONa in MeOH and DMSO. PCB congeners. The total content of all hydroxy derivatives was 77.2%. It was established that the strain Rhodococcus wratislaviensis KT112-7 carries out the destruction of 73-93% of this mixture at the initial concentration of 0.25-1.50 g/l. The best result was obtained with a decomposition of 0.1 g/l of a mixture of methoxy- and hydroxy-polychlorobiphenyls (on the 10th day the total absence of the starting compounds). The specific rate of destruction was in direct correlation with the initial concentration of the mixture. It has been shown that during bacterial transformation there is no accumulation of toxic intermediate compounds.

scholarly journals Mapping of the pneumococcus chromosome: differences between recipient strains varying inhexproperty and the location of theopt-r2gene

1979 ◽  
Vol 33 (1) ◽  
pp. 1-14 ◽  
Author(s):  
L. O. Butler ◽  
G. Nicholas ◽  
R. W. Grist
Keyword(s):  
Linkage Studies ◽  
Chromosome Map ◽  
Competent Cells ◽  
Chromosome Arm

SUMMARYTransformation studies in pneumococcus had shown that loci determining the resistance to erythromycin and streptomycin were unlinked when strain Cl3 was recipient but linked when strain SIII-I was recipient. This phenomenon also applies to other pairs of markers studied in these two recipients, no matter whether the transforming DNA was derived from strain Cl3 or strain SIII-I. Other differences between the two recipient strains were also revealed. Whereas competent cultures of strain Cl3 were composed of all competent cells, which was in agreement with previous reports of pneumococcal cultures, strain SIII-I normally gave a maximum average of 28% competent cells. Strain SIII-I was unstable, since on repeated sub-culturing the competence peak profile changed and the value of ‘fcq’ increased. These properties were reflected in the twohex−strains 401 and R6x which were found to be similar to the ‘altered SIII-I’ strain. The results from the linkage studies have been applied to the chromosome map and have placed theopt-r2gene in thestr-r41-containing chromosome arm.

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