scholarly journals Cell migration assay or Transwell assay v1 (protocols.io.bgd9js96)

protocols.io ◽  
2020 ◽  
Author(s):  
Marzia Ognibene
Keyword(s):  
Cell Migration ◽  
Transwell Assay ◽  
Cell Migration Assay ◽  
Migration Assay

Effect of alginate on the properties of hydroxyapatite scaffold and cell migration assay study

2019 ◽  
Vol 17 ◽  
pp. 820-828
Author(s):  
M.I. Mazlam ◽  
N.A Kamalaldin ◽  
Q.X. Ong ◽  
B.H Yahya ◽  
A. Nurazreena
Keyword(s):  
Cell Migration ◽  
Cell Migration Assay ◽  
Migration Assay ◽  
Hydroxyapatite Scaffold

scholarly journals A Quantitative, Facile, and High-Throughput Image-Based Cell Migration Method Is a Robust Alternative to the Scratch Assay

2011 ◽  
Vol 16 (2) ◽  
pp. 155-163 ◽  
Author(s):  
Wendy Gough ◽  
Keren I. Hulkower ◽  
Renee Lynch ◽  
Patrick Mcglynn ◽  
Mark Uhlik ◽  
...  
Keyword(s):  
Cell Migration ◽  
Kinase Inhibitor ◽  
Cell Monolayer ◽  
Cost Effective ◽  
Statistical Validation ◽  
Scratch Assay ◽  
Cell Migration Assay ◽  
Migration Assay ◽  
Confluent Cell ◽  
Releasing Factors

Cell migration is a key phenotype for a number of therapeutically important biological responses, including angiogenesis. A commonly used method to assess cell migration is the scratch assay, which measures the movement of cells into a wound made by physically scoring a confluent cell monolayer to create an area devoid of cells. Although this method has been adequate for qualitative characterization of migration inhibitors, it does not provide the highly reproducible results required for quantitative compound structure-activity relationship evaluation because of the inconsistent size and placement of the wound area within the microplate well. The Oris™ Cell Migration Assay presents a superior alternative to the scratch assay, permitting formation of precisely placed and homogeneously sized cell-free areas into which migration can occur without releasing factors from wounded or dead cells or damaging the underlying extracellular matrix. Herein the authors compare results from the scratch and Oris™ cell migration assays using an endothelial progenitor cell line and the Src kinase inhibitor dasatinib. They find that using the Acumen™ Explorer laser microplate cytometer in combination with the Oris™ Cell Migration Assay plate provides a robust, efficient, and cost-effective cell migration assay exhibiting excellent signal to noise, plate uniformity, and statistical validation metrics.

Multi-scale modeling of a wound-healing cell migration assay

2007 ◽  
Vol 245 (3) ◽  
pp. 576-594 ◽  
Author(s):  
Anna Q. Cai ◽  
Kerry A. Landman ◽  
Barry D. Hughes
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Cell Migration Assay ◽  
Migration Assay ◽  
Multi Scale ◽  
Scale Modeling ◽  
Multi Scale Modeling
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