An automatic and quantitative on-chip cell migration assay using self-assembled monolayers combined with real-time cellular impedance sensing

Lab on a Chip ◽  
2008 ◽  
Vol 8 (6) ◽  
pp. 872 ◽  
Author(s):  
Lei Wang ◽  
Jing Zhu ◽  
Cheng Deng ◽  
Wan-li Xing ◽  
Jing Cheng
Keyword(s):  
Cell Migration ◽  
Real Time ◽  
Self Assembled Monolayers ◽  
Impedance Sensing ◽  
Cell Migration Assay ◽  
Migration Assay ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
On Chip

Real-Time Detection of Per-fluoroalkyl Substance (PFAS) Self-Assembled Monolayers in Nanoporous Interferometers

2021 ◽  
pp. 131340
Author(s):  
Cheryl Suwen Law ◽  
Juan Wang ◽  
Satyathiran Gunenthiran ◽  
Siew Yee Lim ◽  
Andrew D. Abell ◽  
...  
Keyword(s):  
Real Time ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Real Time Detection ◽  
Self Assembled

Analysis of Self-Assembled Monolayers on Gold Surfaces Using Direct Analysis in Real Time Mass Spectrometry

2007 ◽  
Vol 79 (14) ◽  
pp. 5479-5483 ◽  
Author(s):  
Kafui Kpegba ◽  
Tycho Spadaro ◽  
Robert B. Cody ◽  
Nasri Nesnas ◽  
Joel A. Olson
Keyword(s):  
Mass Spectrometry ◽  
Real Time ◽  
Direct Analysis ◽  
Self Assembled Monolayers ◽  
Gold Surfaces ◽  
Assembled Monolayers ◽  
Self Assembled

On-chip cell migration assay using microfluidic channels

Biomaterials ◽  
2007 ◽  
Vol 28 (27) ◽  
pp. 4017-4022 ◽  
Author(s):  
F NIE ◽  
M YAMADA ◽  
J KOBAYASHI ◽  
M YAMATO ◽  
A KIKUCHI ◽  
...  
Keyword(s):  
Cell Migration ◽  
Microfluidic Channels ◽  
Cell Migration Assay ◽  
Migration Assay ◽  
On Chip

scholarly journals Impact of Self-Assembled Monolayer Design and Electrochemical Factors on Impedance-Based Biosensing

Sensors ◽  
2020 ◽  
Vol 20 (8) ◽  
pp. 2246 ◽  
Author(s):  
Michael C. Brothers ◽  
David Moore ◽  
Michael St. Lawrence ◽  
Jonathan Harris ◽  
Ronald M. Joseph ◽  
...  
Keyword(s):  
Charge Density ◽  
Real Time ◽  
Electrostatic Interactions ◽  
Electrochemical Sensing ◽  
Single Frequency ◽  
Self Assembled Monolayers ◽  
Self Assembled Monolayer ◽  
Frequency Measurements ◽  
Assembled Monolayers ◽  
Self Assembled

Real-time sensing of proteins, especially in wearable devices, remains a substantial challenge due to the need to convert a binding event into a measurable signal that is compatible with the chosen analytical instrumentation. Impedance spectroscopy enables real-time detection via either measuring electrostatic interactions or electron transfer reactions while simultaneously being amenable to miniaturization for integration into wearable form-factors. To create a more robust methodology for optimizing impedance-based sensors, additional fundamental studies exploring components influencing the design and implementation of these sensors are needed. This investigation addresses a sub-set of these issues by combining optical and electrochemical characterization to validate impedance-based sensor performance as a function of (1) biorecognition element density, (2) self-assembled monolayer chain length, (3) self-assembled monolayer charge density, (4) the electrochemical sensing mechanism and (5) the redox reporter selection. Using a pre-existing lysozyme aptamer and lysozyme analyte combination, we demonstrate a number of design criteria to advance the state-of-the-art in protein sensing. For this model system we demonstrated the following: First, denser self-assembled monolayers yielded substantially improved sensing results. Second, self-assembled monolayer composition, including both thickness and charge density, changed the observed peak position and peak current. Third, single frequency measurements, while less informative, can be optimized to replace multi-frequency measurements and in some cases (such as that with zwitterionic self-assembled monolayers) are preferred. Finally, various redox reporters traditionally not used in impedance sensing should be further explored. Collectively, these results can help limit bottlenecks associated with device development, enabling realization of next-generation impedance-based biosensing with customize sensor design for the specific application.

Electrochemical behavior of self-assembled monolayers based on functionalized oligothiophenes

1998 ◽  
Vol 95 (6) ◽  
pp. 1339-1342 ◽  
Author(s):  
R. Michalitsch ◽  
A. El Kassmi ◽  
P. Lang ◽  
A. Yassar ◽  
F. Garnier
Keyword(s):  
Electrochemical Behavior ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled

Imaging of patterned self-assembled monolayers by scanning photoelectron microscopy

2003 ◽  
Vol 104 ◽  
pp. 459-462 ◽  
Author(s):  
R. Klauser ◽  
M. Zharnikov ◽  
I.-H. Hong ◽  
S.-C. Wang ◽  
A. Gölzhäuser ◽  
...  
Keyword(s):  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Scanning Photoelectron Microscopy ◽  
Self Assembled
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