scholarly journals Preparation of 5M Guanidine thiocyanate L6 Inactivation Buffer v1 (protocols.io.bfevjje6)

protocols.io ◽  
2020 ◽  
Author(s):  
Nicola O ◽  
Svend Kjaer
Keyword(s):  
Guanidine Thiocyanate

scholarly journals High-quality total RNA isolation from melon (Cucumis melo L.) fruits rich in polysaccharides

2017 ◽  
Vol 38 (4) ◽  
pp. 2201 ◽  
Author(s):  
Gabrielle Silveira de Campos ◽  
Ricardo Antônio Ayub ◽  
Rafael Mazer Etto ◽  
Carolina Weigert Galvão ◽  
Marília Aparecida Stroka ◽  
...  
Keyword(s):  
Rna Extraction ◽  
Sodium Perchlorate ◽  
Rna Isolation ◽  
World Market ◽  
Molecular Techniques ◽  
Guanidine Thiocyanate ◽  
High Quality ◽  
Total Rna ◽  
High Concentrations ◽  
Cucumis Melo L

Melon, a member of the family Cucurbitaceae, is the fourth most important fruit in the world market and, on a volume basis, is Brazil’s main fresh fruit export. Many molecular techniques used to understand the maturation of these fruits require high concentrations of highly purified RNA. However, melons are rich in polyphenolic compounds and polysaccharides, which interfere with RNA extraction. This study aimed to determine the most appropriate method for total RNA extraction from melon fruits. Six extraction buffers were tested: T1) guanidine thiocyanate/phenol/chloroform; T2) sodium azide/?-mercaptoethanol; T3) phenol/guanidine thiocyanate; T4) CTAB/PVP/?-mercaptoethanol; T5) SDS/sodium perchlorate/PVP/?-mercaptoethanol, and T6) sarkosyl/PVP/guanidine thiocyanate, using the AxyPrepTM Multisource Total RNA Miniprep Kit. The best method for extracting RNA from both mature and green fruit was based on the SDS/PVP/?-mercaptoethanol buffer, because it rapidly generated a high quality and quantity of material. In general, higher amounts of RNA were obtained from green than mature fruits, probably due to the lower concentration of polysaccharides and water. The purified material can be used as a template in molecular techniques, such as microarrays, RT-PCR, and in the construction of cDNA and RNA-seq data.

Severe Toxicity with Guanidine Thiocyanate Ingestion

2016 ◽  
Vol 06 (05) ◽  
Author(s):  
Colin B Page ◽  
Peter S Kruger ◽  
Goce Dimeski ◽  
James Walsham
Keyword(s):  
Severe Toxicity ◽  
Guanidine Thiocyanate

scholarly journals High stability of faecal microbiome composition in guanidine thiocyanate solution at room temperature and robustness during colonoscopy

Gut ◽  
2016 ◽  
Vol 65 (9) ◽  
pp. 1574-1575 ◽  
Author(s):  
Yuichiro Nishimoto ◽  
Sayaka Mizutani ◽  
Takeshi Nakajima ◽  
Fumie Hosoda ◽  
Hikaru Watanabe ◽  
...  
Keyword(s):  
High Stability ◽  
Room Temperature ◽  
Guanidine Thiocyanate ◽  
Microbiome Composition ◽  
Thiocyanate Solution ◽  
Faecal Microbiome

scholarly journals Structural Perturbation of Superfolder GFP in the Presence of Guanidine Thiocyanate

2012 ◽  
Vol 27 ◽  
pp. 381-386
Author(s):  
Olesya V. Stepanenko ◽  
Olga V. Stepanenko ◽  
Irina M. Kuznetsova ◽  
Vladyslav V. Verkhusha ◽  
Konstantin K. Turoverov
Keyword(s):  
Absorption Spectrum ◽  
Fluorescence Intensity ◽  
Tryptophan Fluorescence ◽  
Visible Absorption Spectrum ◽  
Guanidine Thiocyanate ◽  
Native Structure ◽  
Visible Absorption ◽  
Structural Perturbation ◽  
Structural Disturbances

The guanidine-thiocyanate-induced denaturation-renaturation of sfGFP was studied. It was shown that the disruption of sfGFP native structure occurs in the range of guanidine thiocyanate concentrations from 0.5 to 2.5 M. This process was accompanied by simultaneous changes of all recorded parameters. It was found that the small guanidine thiocyanate concentrations (less then 0.1-0.2 M) triggered local structural disturbances in protein which result in significant decrease of chromophore and tryptophan fluorescence intensity and change of protein visible absorption spectrum.

Determination, by dynamic surface-tension analysis, of the molar mass of proteins denatured in guanidine thiocyanate

2004 ◽  
Vol 378 (1) ◽  
pp. 134-143 ◽  
Author(s):  
Wes W. C. Quigley ◽  
Emilia Bramanti ◽  
Bethany A. Staggemeier ◽  
Keith E. Miller ◽  
Abdul Nabi ◽  
...  
Keyword(s):  
Surface Tension ◽  
Molar Mass ◽  
Dynamic Surface Tension ◽  
Guanidine Thiocyanate ◽  
Dynamic Surface ◽  
Tension Analysis

Preservation of nucleic acid integrity in guanidine thiocyanate lysates of whole blood

2006 ◽  
Vol 32 (6) ◽  
pp. 547-551 ◽  
Author(s):  
A. V. Kravchenko ◽  
E. V. Chetverina ◽  
A. B. Chetverin
Keyword(s):  
Nucleic Acid ◽  
Whole Blood ◽  
Guanidine Thiocyanate

scholarly journals Distinct Effects of Guanidine Thiocyanate on the Structure of Superfolder GFP

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e48809 ◽  
Author(s):  
Olesya V. Stepanenko ◽  
Olga V. Stepanenko ◽  
Irina M. Kuznetsova ◽  
Daria M. Shcherbakova ◽  
Vladislav V. Verkhusha ◽  
...  
Keyword(s):  
Guanidine Thiocyanate
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