scholarly journals Structural Perturbation of Superfolder GFP in the Presence of Guanidine Thiocyanate

2012 ◽  
Vol 27 ◽  
pp. 381-386
Author(s):  
Olesya V. Stepanenko ◽  
Olga V. Stepanenko ◽  
Irina M. Kuznetsova ◽  
Vladyslav V. Verkhusha ◽  
Konstantin K. Turoverov
Keyword(s):  
Absorption Spectrum ◽  
Fluorescence Intensity ◽  
Tryptophan Fluorescence ◽  
Visible Absorption Spectrum ◽  
Guanidine Thiocyanate ◽  
Native Structure ◽  
Visible Absorption ◽  
Structural Perturbation ◽  
Structural Disturbances

The guanidine-thiocyanate-induced denaturation-renaturation of sfGFP was studied. It was shown that the disruption of sfGFP native structure occurs in the range of guanidine thiocyanate concentrations from 0.5 to 2.5 M. This process was accompanied by simultaneous changes of all recorded parameters. It was found that the small guanidine thiocyanate concentrations (less then 0.1-0.2 M) triggered local structural disturbances in protein which result in significant decrease of chromophore and tryptophan fluorescence intensity and change of protein visible absorption spectrum.

scholarly journals Direct observation of o-benzyne formation in photochemical hexadehydro-Diels–Alder (hν-HDDA) reactions

2020 ◽  
Vol 11 (34) ◽  
pp. 9198-9208 ◽  
Author(s):  
Xiaonan Ma ◽  
Jan Maier ◽  
Michael Wenzel ◽  
Alexandra Friedrich ◽  
Andreas Steffen ◽  
...  
Keyword(s):  
Absorption Spectrum ◽  
Direct Observation ◽  
Diels Alder ◽  
Visible Absorption Spectrum ◽  
Visible Absorption ◽  
First Time

We report the transient ultraviolet/visible absorption spectrum of an o-benzyne species in solution for the first time.

Authentication and Analysis of Olive Oil Based on Stimulated Brillouin Scattering and UV-Visible Absorption Spectrum

2018 ◽  
Vol 45 (9) ◽  
pp. 0911016
Author(s):  
曹晨鹏 Cao Chenpeng ◽  
郝仕国 Hao Shiguo ◽  
罗宁宁 Luo Ningning ◽  
史久林 Shi Jiulin ◽  
何兴道 He Xingdao
Keyword(s):  
Absorption Spectrum ◽  
Olive Oil ◽  
Brillouin Scattering ◽  
Stimulated Brillouin Scattering ◽  
Visible Absorption Spectrum ◽  
Visible Absorption ◽  
Uv Visible ◽  
Stimulated Brillouin

scholarly journals The visible absorption spectrum of water.

1980 ◽  
Vol 56 (7) ◽  
pp. 403-407 ◽  
Author(s):  
Hiroshi TSUBOMURA ◽  
Naoto YAMAMOTO ◽  
Nobutaka MATSUO ◽  
Yoshio OKADA
Keyword(s):  
Absorption Spectrum ◽  
Visible Absorption Spectrum ◽  
Visible Absorption

scholarly journals Interaction of cytochrome b5 with surfactant vesicles

1988 ◽  
Vol 251 (2) ◽  
pp. 391-396
Author(s):  
D M Davies ◽  
J M Lawther
Keyword(s):  
Absorption Spectrum ◽  
Phosphatidic Acid ◽  
Cytochrome B5 ◽  
Visible Absorption Spectrum ◽  
Visible Absorption ◽  
Surfactant Vesicles ◽  
Dimyristoyl Phosphatidylcholine ◽  
Dicetyl Phosphate ◽  
Kinetics Of

Changes in the u.v.-visible absorption spectrum of cytochrome b5 which occur upon addition of dicetyl phosphate, dimyristoyl phosphatidic acid (DMPA), or mixed DMPA/dimyristoyl phosphatidylcholine vesicles are consistent with haem transfer from the cytochrome to the membrane via an intermediate haemoprotein species. The effects of vesicle charge and concentration on the kinetics of haem transfer are discussed.

scholarly journals Effect of Cu, Cr, S Doped TiO2 for Transparent Plastic Bar Reinforced Concrete

2020 ◽  
Vol 10 (20) ◽  
pp. 7334
Author(s):  
Seung-Hoon Seo ◽  
Byoung-Il Kim
Keyword(s):  
Absorption Spectrum ◽  
Reinforced Concrete ◽  
Visible Light ◽  
High Performance Concrete ◽  
Test Method ◽  
Visible Absorption Spectrum ◽  
Nox Removal ◽  
Transparent Plastic ◽  
Visible Absorption ◽  
Uv Visible

In this study, after firing and powdering Cu, Cr, and S with NP-400 TiO2, an NOx removal rate test was performed according to the ISO test method to analyze the photocatalytic reactivity in visible light. The distribution of the photocatalyst and visible light reactivity on the surface of the test specimen were confirmed through SEM (Scanning electron microscope), EDS (Energy dispersive spectroscopy), XRD (X-ray diffraction), UV-visible absorption spectrum and energy band gap tests. The flowability of UHPC (Ultra high performance concrete) slightly decreased due to the increase in the photocatalyst mixing rate, but both J-Ring and L-Box test results showed that there is no problem when concrete is placed. As a result of SEM and EDS tests, good microstructure and peak values were confirmed in the test specimens doped with Cu, and as a result of the XRD test, anatase and rutile peaks were confirmed in the Cu, Cr, and S specimens. In the UV-visible absorption spectrum analysis, it was confirmed that only the specimen doped with Cu maintains a high absorption power of 0.8 up to 700 nm, and the inherent band gaps are reduced to 2.9 eV, thereby increasing the possibility of reaction in visible light. Finally, as a result of the NOx removal test by the ISO test method, about 15.8% was removed for 5 h in the specimen doped with Cu, and the removal efficiency is estimated to be possible up to about 25% when applied with the TPBRC (transparent plastic bar reinforced concrete).

The visible absorption spectrum of 1,2-cyclobutanedione in the gas phase

1986 ◽  
Vol 64 (11) ◽  
pp. 2152-2161 ◽  
Author(s):  
R. A. Back ◽  
J. M. Parsons
Keyword(s):  
Absorption Spectrum ◽  
Gas Phase ◽  
Singlet State ◽  
Energy Levels ◽  
Vibrational Structure ◽  
Visible Absorption Spectrum ◽  
Excited Singlet ◽  
Bending Vibration ◽  
Visible Absorption ◽  
Out Of Plane

The visible absorption spectrum of 1,2-cyclobutanedione has been measured in the gas phase at wavelengths between 4000 and 5100 Å. The absorption is attributed to the allowed π* ← n+, 1B1 ← 1A1 transition corresponding to the first excited singlet state. The spectrum shows a complex well-resolved vibrational structure which has been analysed, with some 125 bands measured and assigned. The bands at the longer wavelengths show sharp rotational fine structure, not yet analysed. The strongest band in the spectrum at 4933 Å has been assigned as the 0–0 band, while a band almost as strong at 4820 Å is attributed to excitation of one quantum of [Formula: see text], the a2 out-of-plane carbonyl bending vibration, and it is suggested that this band owes its intensity to vibronic coupling. A number of symmetric vibrations are also excited in the spectrum, but with no long progressions. Sequence bands running to the blue with an interval of about 72 cm−1 are prominent throughout the spectrum, and are assigned to v13, the a2 ring-twisting vibration. Other hot bands were also observed involving v13 which permitted estimation of energy levels for this vibration both in the ground state and the excited state. The infrared spectrum was also measured and analysed in the gas phase between 600 and 4000 cm−1, and 14 bands were assigned to fundamental vibrations; some of these assignments, at the lower frequencies, are uncertain.

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