scholarly journals Sperm morphology, ultrastructure, and motility in pikeperch Sander lucioperca (Percidae, Teleostei) associated with various activation media 

2014 ◽  
Vol 59 (No. 1) ◽  
pp. 1-10 ◽  
Author(s):  
J. Křišťan ◽  
A. Hatef ◽  
S.M.H. Alavi ◽  
T. Policar
Keyword(s):  
Sperm Morphology ◽  
Storage Period ◽  
Spherical Nucleus ◽  
Sander Lucioperca ◽  
Central Pair ◽  
Spermatozoa Motility ◽  
Sampling Times ◽  
Freshwater Teleosts

Spermatozoa morphology, ultrastructure, and spermatozoa motility traits were studied in pikeperch (Sander lucioperca) after activation in various media (AM 1 &ndash; 45mM NaCl, 5mM KCl, 20mM Tris, pH 8.5; AM 2 &ndash; 100mM sucrose, 20mM Tris, pH 8.5; AM 3 &ndash; 100mM sucrose, 1mM CaCl<sub>2</sub>, 20mM Tris, pH 8.5) during a 48-hour storage period. The spermatozoon was acrosomeless and differentiated into a spherical nucleus (head), midpiece, and flagellum. The nucleus length and width measured 1.83 &plusmn; 0.03 and 1.63 &plusmn; 0.02&nbsp;mm, respectively. The midpiece was located laterally to the nucleus and possessed proximal and distal centrioles and 2&ndash;4 mitochondria. Flagellar length was 33.2 &plusmn; 0.90 &micro;m, and a pair of lateral fin-like structures projections was observed. The axoneme consisted of nine peripheral doublet microtubules and a single central pair. After a 24 h storage in all activation media at all sampling times post-activation (15, 45, 90, and 120 s), spermatozoa motility was significantly decreased. Spermatozoa were motile after the 48-hour storage at all sampling times post-activation only in AM 3. After the 48-hour storage, no motile spermatozoa were observed in AM 2 and AM 1 at 90 and 120 s post-activation, respectively. Differences in spermatozoa velocity varied with activation medium during storage. After the 48-hour storage in AM 1 and AM 2, decrease of spermatozoa velocity at 15 s post-activation was observed, while in AM 3, velocity was decreased only after the 48-hour storage. Pikeperch spermatozoa morphology and ultrastructure was found similar to that of most freshwater teleosts, with differences in the arrangement of midpiece, number of mitochondria, and position of centrioles. Viable pikeperch sperm was observed after the 48-hour storage. Motility of spermatozoa was improved by addition of Ca<sup>2+</sup> to the activation medium, where higher spermatozoa velocity was observed.

scholarly journals Digestive enzyme system of larvae of different freshwater teleosts and its differentiation during the initial phase of exogenous feeding

2017 ◽  
Vol 62 (No. 10) ◽  
pp. 403-416 ◽  
Author(s):  
F. Lahnsteiner
Keyword(s):  
Digestive System ◽  
Enzyme System ◽  
Digestive Enzyme ◽  
Sander Lucioperca ◽  
Thymallus Thymallus ◽  
Exogenous Feeding ◽  
First Feeding ◽  
Histological Characteristics ◽  
Species Specific ◽  
Freshwater Teleosts

Activities of digestive enzymes and main histological characteristics of the intestine were investigated in larvae of three salmonid species (Coregonus maraena, C. atterensis, Thymallus thymallus), of burbot (Lota lota), and pikeperch (Sander lucioperca) at the onset of exogenous feeding (0 day degrees (°d)) and at 250°d thereafter. At the onset of exogenous feeding the activities of proteolytic, lipolytic, and carbohydrate splitting enzymes were detected in the intestines of all species. The enzymatic activities showed significant species specific differences indicating specializations in functionality and digestion ability. In C. atterensis and L. lota the activities of most enzymes were low in comparison to the other investigated species and therefore their digestive system was only poorly developed. In S. lucioperca it was moderately developed and in T. thymallus and C. maraena well-developed. After 250°d, the activities of the investigated enzymes changed in a very species specific way. Histologically, the intestines of the investigated species revealed no species specific differentiations at the onset of the first feeding with the exception of the absence of goblet cells in L. lota and C. atterensis. These differentiated after 250°d.

Detection of Guaiacol Produced by Alicyclobacillus acidoterrestris in Apple Juice by Sensory and Chromatographic Analyses, and Comparison with Spore and Vegetative Cell Populations

2000 ◽  
Vol 63 (11) ◽  
pp. 1517-1522 ◽  
Author(s):  
RACHEL V. ORR ◽  
ROBERT L. SHEWFELT ◽  
C. J. HUANG ◽  
SEBHAT TEFERA ◽  
LARRY R. BEUCHAT
Keyword(s):  
Apple Juice ◽  
Fruit Juice ◽  
Storage Period ◽  
Storage Conditions ◽  
Sensory Panel ◽  
Alicyclobacillus Acidoterrestris ◽  
Concentration Method ◽  
Processing And Storage ◽  
And Storage ◽  
Sampling Times

Spoilage of fruit juice by Alicyclobacillus acidoterrestris is characterized by a distinct medicinal or antiseptic off odor attributed to guaiacol, a metabolic by product of the bacterium. Detection of low populations of A. acidoterrestris that would precede sensory detection of guaiacol would enable juice processors to select appropriate processing and storage conditions that would minimize or eliminate spoilage. The objective of this study was to determine the recognition threshold of guaiacol in apple juice by sensory analysis and the population of A. acidoterrestris and incubation time at 21 and 37°C necessary for chemical detection of guaiacol. Commercially sterilized apple juice (pH 3.54 ± 0.04, 11.3 ± 0.3°Brix) was inoculated with a five-strain mixture of A. acidoterrestris spores (2.98 log10 CFU/ml) and stored at 21 or 37°C for up to 61 days. Using an experienced sensory panel and the forced-choice ascending concentration method of limits, the best estimate threshold (BET) for recognition of guaiacol added to uninoculated apple juice was 2.23 ppb. Numbers of A. acidoterrestris spores and cells in inoculated juice remained constant during the 61-day storage period; however, the panel detected (P ≤ 0.01) guaiacol in juice stored at 37°C within 8 days. At three of four sampling times ranging from 13 to 61 days at which the sensory panel detected (P ≤ 0.001) guaiacol, concentrations of 8.1 to 11.4 ppb were detected by chromatographic analysis. The panel detected (P ≤ 0.1 to P ≤ 0.01) guaiacol in five samples stored at 21 to 37°C for 8 to 61 days in which the compound was not detected by chromatographic analyses. It appears that guaiacol content in apple juice inoculated with A. acidoterrestris is not always correlated with numbers of cells, and the limit of sensitivity of chromatographic quantitation of the compound is higher than the BET.

THE EFFECT OF SODIUM PENTOBARBITAL AND STORAGE TIME ON PRESERVATION OF RAM SPERMATOZOA MOTILITY AT 5 °C

1981 ◽  
Vol 61 (4) ◽  
pp. 847-851 ◽  
Author(s):  
P. S. FISER ◽  
G. A. LANGFORD
Keyword(s):  
Storage Time ◽  
Storage Period ◽  
Sodium Pentobarbital ◽  
Spermatozoa Motility ◽  
The Difference ◽  
And Storage

The effect of sodium pentobarbital (0, 50, 100, 150, 200 and 300 mg/L of diluent) on the survival of ram spermatozoa stored at 5 °C for up to 7 days was studied. Although spermatozoa motility in diluent containing 150 mg of sodium pentobarbital was higher [Formula: see text] than in the remaining diluents, the magnitude of the difference was small. The average motility percent and rating of 73% and 4.0 for all sodium pentobarbital concentrations observed at the beginning of the storage period declined gradually to 22% and 2.1, respectively, after 7 days of storage. The pregnancy and lambing rates of 70% and 63.3% in ewes inseminated at synchronized estrus with semen extended and stored for 24 h with sodium pentobarbital (150 mg/L of diluent) did not differ significantly (P > 0.05) from the respective rates of 73% and 66.7% in the control ewes.

Sperm ultrastructure in two species of Brachidontes (Bivalvia, Mytilidae) from the south-western Atlantic Ocean

2015 ◽  
Vol 95 (5) ◽  
pp. 991-998 ◽  
Author(s):  
María Eugenia Torroglosa ◽  
Juliana Giménez
Keyword(s):  
Sperm Morphology ◽  
Spherical Nucleus ◽  
Rocky Shores ◽  
Sperm Ultrastructure ◽  
Intertidal Area ◽  
Western Atlantic ◽  
Transmission Electron ◽  
Anterior Extension ◽  
Wide Geographic Distribution ◽  
Mature Spermatozoa

Brachidontes rodriguezii and Brachidontes purpuratus have a wide geographic distribution along the Argentinean coast. Both species are abundant in the intertidal area of rocky shores. We used transmission electron microscopy to study the spermatogenesis of B. rodriguezii and the structure of mature spermatozoa from B. rodriguezii and B. purpuratus. Our results show that spermatogenesis in B. rodriguezii is very similar to that reported in other mytilids. The spermatozoa of both Brachidontes are of the primitive or ect-aquasperm type showing a spherical nucleus capped by a conical acrosome with an anterior extension. The chromatin was electron-dense, homogeneous and compact. The mid-piece region consisted of 5 spherical mitochondria grouped in a ring around a pair of short cylindrical centrioles. The flagellum exhibit the typical 9 + 2 microtubule structure. Studies of spermatozoa ultrastructure are considered a useful tool in bivalve phylogeny. Characters in the spermatozoa morphology of these two species were observed and a brief discussion about the sperm morphology along the distribution of both species is presented. We suggest that the acrosome complex presents the most significant differences between both species.

119: Effect of Epididymitis on Semen Biochemical and Sperm Morphology Parameters

2005 ◽  
Vol 173 (4S) ◽  
pp. 32-32
Author(s):  
Petra Huwe ◽  
Roelof Menkveld ◽  
Martin Ludwig ◽  
Wolfgang Weidner
Keyword(s):  
Sperm Morphology ◽  
Morphology Parameters

Photon correlation study of spermatozoa motility

1979 ◽  
Vol 40 (11) ◽  
pp. 1085-1088 ◽  
Author(s):  
B. Herpigny ◽  
J.-P. Boon
Keyword(s):  
Correlation Study ◽  
Photon Correlation ◽  
Spermatozoa Motility

Storage of Human Blood Platelets

1974 ◽  
Vol 32 (02/03) ◽  
pp. 405-416 ◽  
Author(s):  
M. R Hardeman ◽  
Carina J L. Heynens
Keyword(s):  
Storage Temperature ◽  
Platelet Rich Plasma ◽  
Blood Platelets ◽  
Storage Period ◽  
Serotonin Uptake ◽  
Hypotonic Shock ◽  
Glycolytic Intermediates

SummaryStorage experiments were performed at 4°, 25° and 37° C with platelet-rich plasma under sterile conditions. In some experiments also the effect of storing platelets at 4° C in whole blood was investigated.Before, during and after three days of storage, the platelets were tested at 37° C for their serotonin uptake and response to hypotonic shock. In addition some glycolytic intermediates were determined.A fair correlation was noticed between the serotonin uptake and hypotonic shock experiments. Both parameters were best maintained at 25° C. Also platelet counting, performed after the storage period, indicated 25° C as the best storage temperature. Determination of glycolytic intermediates did not justify any conclusion regarding the optimal storage temperature. Of the various anticoagulants studied, ACD and heparin gave the best results as to the serotonin uptake and hypotonic shock response, either with fresh or stored platelets. The use of EDTA resulted in the lowest activity, especially after storage.The results of these storage experiments in vitro, correspond well with those in vivo reported in the literature.

EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

2019 ◽  
Vol 14 (1) ◽  
pp. 26
Author(s):  
Dyah Anggraeni ◽  
Nurlela Nurlela
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Research Result ◽  
Clarias Gariepinus ◽  
Storage Period ◽  
The Body ◽  
Garlic Extract ◽  
African Catfish ◽  
Natural Preservatives ◽  
Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L).  Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus).  Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter.  Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

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