THE EFFECT OF SODIUM PENTOBARBITAL AND STORAGE TIME ON PRESERVATION OF RAM SPERMATOZOA MOTILITY AT 5 °C

1981 ◽  
Vol 61 (4) ◽  
pp. 847-851 ◽  
Author(s):  
P. S. FISER ◽  
G. A. LANGFORD
Keyword(s):  
Storage Time ◽  
Storage Period ◽  
Sodium Pentobarbital ◽  
Spermatozoa Motility ◽  
The Difference ◽  
And Storage

The effect of sodium pentobarbital (0, 50, 100, 150, 200 and 300 mg/L of diluent) on the survival of ram spermatozoa stored at 5 °C for up to 7 days was studied. Although spermatozoa motility in diluent containing 150 mg of sodium pentobarbital was higher [Formula: see text] than in the remaining diluents, the magnitude of the difference was small. The average motility percent and rating of 73% and 4.0 for all sodium pentobarbital concentrations observed at the beginning of the storage period declined gradually to 22% and 2.1, respectively, after 7 days of storage. The pregnancy and lambing rates of 70% and 63.3% in ewes inseminated at synchronized estrus with semen extended and stored for 24 h with sodium pentobarbital (150 mg/L of diluent) did not differ significantly (P > 0.05) from the respective rates of 73% and 66.7% in the control ewes.

scholarly journals The Physicochemical and Microbiological Quality of Pegagan Duck Eggs Immersed with Duku Fruit Peel Solution in Different Storage Period

2019 ◽  
Vol 43 (2) ◽  
Author(s):  
Fitra Yosi ◽  
Dyah Wahyuni ◽  
Riswandi . ◽  
Fitri Herasti ◽  
Inda Ayu Lestari ◽  
...  
Keyword(s):  
Immersion Time ◽  
Storage Time ◽  
Microbiological Quality ◽  
Storage Period ◽  
Fruit Peel ◽  
Randomized Design ◽  
The Difference ◽  
And Storage ◽  
Range Test

This study aims to determine the effect of immersing in duku fruit peel solution on physicochemical and microbiological quality of Pegagan duck eggs during storage at room temperature. This study used a complete randomized design (CRD) with a 5x3 factorial patterned. The first factor was the immersion time consisting of 5 levels, namely 0, 15, 30, 45, and 60 min. The second factor was the storage time which includes 3 levels, which were 0, 7 and 14 d. The replication used was 3 times. The observed variables were physicochemical and microbiological quality of egg, including albumen index (AI), yolk index (YI), Haugh unit (HU), moisture content (MC), protein content (PC), fat content (FC),total microbes (TM) and antioxidant activity (AA). The data were processed by analysis of variance, continued with Duncan's multiple range test (DMRT). The results showed that there was a significant interaction (P<0.05) between immersion time and storage time on AI, YI, HU, MC, PC, TM, and AA. Futhermore, the difference of immersion time and storage period was also significantly influenced (P<0.05) to all observed variables including AI, YI, HU, MC, PC, TM, and AA of duck eggs.It can be concluded that the immersion process for 60 min showed the best results on the physical, chemical,

scholarly journals Physiological quality of pink pepper seeds (Schinus terebinthifolia Raddi.) as a function of storage

2021 ◽  
Vol 38 (1) ◽  
pp. 144-157
Author(s):  
Thaísa De Oliveira Silveira ◽  
Madelon Rodrigues Braz ◽  
Gilmara Pires de Moura Palermo ◽  
Tiago Böer Breier
Keyword(s):  
Moisture Content ◽  
Storage Time ◽  
Storage Period ◽  
Seed Storage ◽  
Dry Weight ◽  
Native Forest ◽  
Alternative Source ◽  
Physiological Quality ◽  
And Storage

Seeds of native forest species for food and seedling production have a growing demand that impacts the need for studies involving seed storage in order to maintain good levels of germination quality. Thus, scientific analyzes that explore the physiological potential of the seeds of these species are necessary, especially for the pink pepper (Schinus terebinthifolia Raddi.), a species used as an alternative source of income for traditional communities and which has been arousing interest due to the consumption of its fruits as a condiment in the national and international market. This work aimed to assess the physiological quality of pink pepper seeds according to the storage period and seed size. The seeds came from a rural settlement, a pioneer in the extraction of pink pepper, in the state of Rio de Janeiro, Brazil. The seeds were evaluated for moisture content, germination, first count, length and dry weight of the germinated seedlings, during the period of time that they remained stored and the size of the seeds. The results allowed to conclude that: i) the germinative percentage of the stored pink pepper seeds decreased over the months; ii) the moisture content of the seeds decreased over the months evaluated iii) the different sizes of seeds did not show differences in germination and storage time and iv) values of length and dry weight of the seedlings did not differ according to the period of storage.

scholarly journals Analysis of Vitamin C Content of Red Dragon Fruit (Hylocereus polyrhizuz) and White Dragon Fruit (Hylocereus undatus) in Storage with Different Temperatures and Times

2018 ◽  
Vol 5 (2) ◽  
pp. 249
Author(s):  
N. Putri Sumaryani ◽  
Anak Agung Istri Mirah Dharmadewi
Keyword(s):  
Vitamin C ◽  
Storage Time ◽  
Temperature Treatment ◽  
Randomized Design ◽  
Dragon Fruit ◽  
Different Temperatures ◽  
Completely Randomized Design ◽  
The Difference ◽  
Hylocereus Undatus ◽  
And Storage

This study was conducted to determine the temperature and storage time of the exact vitamin C content of red and white dragon fruit and the difference of vitamin C. The samples were taken from the plantation land in Ubud Andong Highway, Petulu, Gianyar Regency. Treated for 10 and 30 days with 2, 10, 20 and 300C temperature treatment with five repetitions using Completely Randomized Design Factorial pattern. Data obtained statistically using ANOVA (Analysis of Variance). The highest vitamin C content in red dragon fruit is 29.61 mg/ 100g temperature 100C for 10 days and the lowest is in 30 days storage with temperature 300C that is equal to 22,09 mg/ 100g. While the white dragon fruit content of vitamin C highest contained in storage with a temperature of 20C is 22.69 mg/ 100g with maximum storage of 10 days and the lowest at a temperature of 300C is 17.37 mg/ 100g.

Method of quantifying the loss of acidification activity of lactic acid starters during freezing and frozen storage

2000 ◽  
Vol 67 (1) ◽  
pp. 83-90 ◽  
Author(s):  
FERNANDA FONSECA ◽  
CATHERINE BÉAL ◽  
GEORGES CORRIEU
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Storage Time ◽  
Frozen Storage ◽  
Operating Conditions ◽  
Linear Relationships ◽  
Before And After ◽  
Acidification Activity ◽  
The Difference ◽  
And Storage

We have developed a method to quantify the resistance to freezing and frozen storage of lactic acid starters, based on measuring the time necessary to reach the maximum acidification rate in milk (tm) using the Cinac system. Depending on the operating conditions, tm increased during the freezing step and storage. The loss of acidification activity during freezing was quantified by the difference (Δtm) between the tm values of the concentrated cell suspension before and after freezing. During storage at −20 °C, linear relationships between tm and the storage time were established. Their slope, k, allowed the quantitation of the decrease in acidification activity during 9–14 weeks of frozen storage. The method was applied to determine the resistance to freezing and frozen storage of four strains of lactic acid bacteria and to quantify the cryoprotective effect of glycerol.

scholarly journals Pengaruh Media Dan Periode Simpan Terhadap Viabilitas Benih Cengkeh Tuni (Syzygium aromaticum L.)

2018 ◽  
Vol 14 (2) ◽  
pp. 97-104
Author(s):  
Marselina Noya ◽  
Johan Riry ◽  
Marthini Lesilolo
Keyword(s):  
Storage Time ◽  
Germination Rate ◽  
Storage Period ◽  
Germination Percentage ◽  
Syzygium Aromaticum ◽  
Storage Media ◽  
Randomized Design ◽  
Vigor Index ◽  
The Media ◽  
And Storage

This study aims to examine the influence of the media and the storage period on the viability of clove seeds. The study was conducted at the Ambon Plant Crops Protection and Protection Center laboratory. The study used a factorial completely randomized design with two types of factors and three replications. The storage media consists of 3 levels, namely: Without the storage media, the media for storing rice husk and the media for storing sawdust. The second factor is the storage period consists of 4 levels, namely: 10 days storage, 20 days storage, 30 days storage and 40 days storage. The parameters measured were germination power, germination rate, and vigor index. Storage media and storage period significantly affected germination and vigor indexes but did not significantly affect germination rates. The results showed that the sawdust storage media was a good medium for storing clove seeds (Syzygium aromaticum L.). This can be seen in the results of the average germination of clove seeds stored in powder storage media with a storage time of 10 days giving better results of 85% compared to rice husks at a storage time of 10 days with a germination percentage of 65%.  Keywords: clove seeds, save media, save period   ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh media dan periode simpan terhadap viabilitas benih cengkeh. Penelitian dilaksanakan di laboratorium Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan Ambon. Penelitian menggunakan rancangan acak lengkap pola faktorial dengan dua macam faktor dan tiga kali ulangan. Media simpan terdiri dari 3 taraf yaitu: Tanpa media simpan, media simpan sekam padi dan media simpan serbuk gergaji. Faktor kedua adalah periode simpan terdiri dari 4 taraf yaitu: penyimpanan 10 hari, penyimpanan 20 hari, penyimpanan 30 hari dan penyimpanan 40 hari. Parameter yang diukur adalah daya berkecambah, laju perkecambahan dan indeks vigor. Media simpan dan periode penyimpanan berpengaruh nyata pada persentase perkecambahan dan indeks vigor tetapi tidak berpengaruh nyata pada laju perkecambahan. Hasil penelitian menunjukan bahwa media simpan serbuk gergaji merupakan media yang baik bagi penyimpanan benih cengkeh (Syzygium aromaticum L.). Hal ini terlihat pada hasil rata-rata daya berkecambah benih cengkeh yang disimpan pada media simpan serbuk dengan lama penyimpanan selama 10 hari memberikan hasil yang lebih baik yakni 85% jika dibandingkan dengan sekam padi pada lama penyimpanan 10 hari dengan persentase perkecambahan 65%. Kata Kunci: benih cengkeh, media simpan, periode simpan

scholarly journals Production of value-added broiler nugget enriched with dietary fiber from oat meal

2020 ◽  
Vol 49 (1) ◽  
pp. 71-82
Author(s):  
A Akhter ◽  
MM Hossain ◽  
M Habib ◽  
MA Hashem ◽  
MS Ali
Keyword(s):  
Dietary Fiber ◽  
Wheat Flour ◽  
Crude Protein ◽  
Dry Matter ◽  
Storage Time ◽  
Storage Period ◽  
Broiler Meat ◽  
Overall Acceptability ◽  
A Value ◽  
And Storage

An experiment was conducted to find out the effect of oat meal as a source of dietary fiber to formulate enriched broiler nugget. For this purpose, nuggets were prepared into four different groups such as treatment 1: 10% wheat flour nugget, treatment 2: 20% wheat flour nugget, treatment 3:10% oat meal nugget and treatment 4: 20% Oat meal nugget. All parameters were analyzed at 0, 15th and 30th days of storage period. The proximate composition of different nuggets batter was analyzed and highly significant differences were found in dry matter (%) and pH. Dry matter was lower, while pH was higher in control nugget (Treatment 1). Significant differences were found in crude protein (%), ether extract (%) among different nuggets, as well as among different storage time. Significantly higher DM (%), Ash (%) and EE (%) was found in broiler meat with 20% oat meal, while higher crude protein (%) were found in broiler meat+10% wheat flour. Crude protein (%) and DM (%) increased, while Ash (%) and EE (%) decreased with increase of storage time.  The storage period had significant effect on different biochemical (FFA, POV and TBARS value) and microbial (TVC, TCC and TYMC) tests. In all cases, the values were increased with increase of storage time. The surface color (CIE L*, a*, b*) of nuggets of different treatments at different storage period were measured.  No significant differences in b* value were found among broiler meat nuggets and storage time. On the other hand, different types of nuggets and storage period had a significant effect on L* and a* value. Significantly higher a* value was found in broiler meat with 10% wheat flour (T1). In sensory analysis, no significant differences were found in flavor, off-flavor, juiciness, tenderness and overall acceptability among four types of sausages. It could be concluded that addition of oat meal as a source of dietary fiber did not differ the overall acceptability of broiler meat nuggets compare to broiler meat nuggets with wheat flour. Bang. J. Anim. Sci. 2020. 49 (1): 71-82

scholarly journals Retaining Quality in Cut Watermelon: Effects on Sanitizing Wash, Storage Time, and Storage Temperature

HortScience ◽  
1998 ◽  
Vol 33 (4) ◽  
pp. 604f-605
Author(s):  
William G. McGlynn ◽  
Siobhan Reilly
Keyword(s):  
Storage Time ◽  
Storage Temperature ◽  
Deionized Water ◽  
Sodium Hypochlorite Solution ◽  
Microbial Counts ◽  
Plate Counts ◽  
Flavor Development ◽  
The Difference ◽  
And Storage ◽  
Plastic Containers

Watermelons were dipped in either 1000 ppm sodium hypochlorite solution or in deionized water, dried, then cut into chunks of ≈83 × 152 × 51 mm. These were sealed into plastic containers and stored at either 2, 4, or 8 °C. Samples were removed after 3, 7, and 10 days for microbial and quality tests. Chlorine dip reduced average aerobic plate counts by ≈3 log cycles and average coliform counts by nearly 2 log cycles. This may have significant implications for food safety and off-flavor development. The difference in microbial counts persisted for ≈7 days. No clear effect from storage temperature was seen. A trend for lower temperatures to preserve red hue was observed in objective color tests. Texture tests revealed a trend for all melons to become firmer during storage. No clear patterns with respect to storage temperature or sanitizing dip were seen.

scholarly journals PENGARUH WADAH, SUHU DAN WAKTU SIMPAN TERHADAP PERKECAMBAHAN BENIH MENIRAN (Phyllanthus niruri L.)

2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Nurul Husniyati Listyana ◽  
Rahma Widyastuti ◽  
Widyantoro Widyantoro
Keyword(s):  
Seed Germination ◽  
Storage Time ◽  
Storage Temperature ◽  
Storage Period ◽  
Raw Material ◽  
Wild Plant ◽  
Phyllanthus Niruri ◽  
Storage Container ◽  
Genetic Traits ◽  
And Storage

ABSTRACT Meniran (Phyllanthus niruri L.) is a wild plant that used extensively as a raw material for traditional medicine but has not been cultivated yet. Meniran cultivation requires the availability of quality seeds and seedlings to produce the high productivity. The ability of seeds to germinate (seed viability) after the storage period is influenced by several factors both internal and external, including seed water content, genetic traits, initial viability and packaging material, storage temperature and humidity, microorganisms, and human factors.. The study aims to determine the effect of storage containers, storage temperature and time of storage on the power of meniran seed germination. The study was conducted in March-April 2018 at the B2P2TO2T Seed Laboratory. The research design was Factorial Completely Randomized Design with 3 factors and 3 replications. The first factor was a storage container consisting of plastic, paper envelopes and aluminum foil. Second factor was a storage temperature consisting of 0 ° C, -20 ° C and -50 ° C.  The third factor was storage time which consists of 2 weeks, 4 weeks, 6 weeks, 8 weeks and 10 weeks. The results showed that the storage container and storage time had a significantly different effect on meniran seed germination. The storage time does not have a significant effect on germination. Together, the storage container, storage temperature and storage time do not have a significant effect on meniran seed germination. The interaction between the storage temperature and the storage time and the interaction between the storage container and the storage time give significantly different effects on germination. ABSTRAK Meniran (Phyllanthus niruri L.) merupakan tanaman liar yang simplisianya banyak dibutuhkan sebagai bahan baku obat tradisional namun belum dibudidayakan. Budidaya tanaman meniran memerlukan ketersediaan benih dan bibit yang bermutu untuk menghasilkan produktivitas yang tinggi. Kemampuan benih untuk berkecambah (viabilitas benih) setelah masa penyimpanan dipengaruhi oleh beberapa faktor baik internal maupun eksternal, meliputi kadar air benih, sifat genetik, viabilitas awal serta bahan pengemas, suhu dan kelembaban ruang simpan, mikroorganisme, serta faktor manusia. Penelitian bertujuan untuk mengetahui pengaruh wadah simpan, suhu simpan dan waktu simpan terhadap daya perkecambahan benih meniran. Penelitian dilaksanakan pada bulan Maret-April 2018 di Laboratorium Benih B2P2TO2T. Desain penelitian yang digunakan yaitu Rancangan Acak Lengkap Faktorial dengan 3 faktor dan 3 ulangan. Faktor 1 yaitu wadah penyimpanan yang terdiri dari plastik, kertas amplop dan aluminium foil. Faktor 2 yaitu suhu simpan yang terdiri dari 0°C, -20°C dan -50°C. Faktor 3 yaitu waktu penyimpanan yang terdiri dari 2 minggu, 4 minggu, 6 minggu, 8 minggu dan 10 minggu. Hasil penelitian menunjukkan wadah simpan dan waktu simpan memberikan pengaruh yang berbeda signifikan terhadap perkecambahan benih meniran.  Suhu simpan tidak memberikan pengaruh yang berbeda signifikan terhadap perkecambahan. Secara bersama-sama wadah simpan, suhu simpan dan waktu simpan tidak memberikan pengaruh yang berbeda signifikan terhadap perkecambahan benih meniran. Interaksi antara suhu simpan dan waktu simpan serta interaksi antara wadah simpan dan waktu simpan memberikan pengaruh yang berbeda signifikan terhadap perkecambahan.  

Effect of storage time and temperature on infectivity, reproduction and development of Heterorhabditis megidis in Galleria mellonella

Nematology ◽  
2000 ◽  
Vol 2 (6) ◽  
pp. 635-644 ◽  
Author(s):  
Gerrie Wiegers ◽  
Mari I.C. Boff ◽  
Peter Smits
Keyword(s):  
Body Length ◽  
Storage Time ◽  
Galleria Mellonella ◽  
Storage Temperature ◽  
Storage Period ◽  
Reproductive Capacity ◽  
Effect Of Temperature ◽  
Inoculum Level ◽  
Heterorhabditis Megidis ◽  
And Storage

AbstractThe effect of temperature, dose and storage period on the infectivity and development of Heterorhabditis megidis (strain NLH-E 87.3) infective juveniles (IJ) was studied in the laboratory. IJ were stored at 5, 10, 15 and 20°C for a period of up to 70 days (10 weeks). Every second week, mortality, infectivity, reproductive capacity, time to emergence and length of IJ were examined. Galleria mellonella larvae were infested with doses of one and 30 IJ. As the period of storage increased, the number of active nematodes, the infectivity and the reproduction of IJ decreased both at low and high temperatures. Time to first emergence and body length of IJ were significantly affected by time, storage temperature and inoculum level. Independent of the dose and storage periods, highest infectivity and optimal development were observed when IJ were stored at 10 and 15°C. Effets de la durée du stockage et de la température sur le pouvoir infestant, la reproduction et le développement d'Heterorhabditis megidis parasitant Galleria mellonella - Les effets de la température, de la dose d'inoculum et de la durée du stockage sur le pouvoir infestant et le développement des juvéniles infestants (JI) d'Heterorhabditis megidis ont été étudiés au laboratoire. Les JI ont été stockés à 5, 7, 10, 15 et 20°C pendant une période allant jusqu'à 70 jours (10 semaines). Toutes les 2 semaines ont été examinées: la mortalité, le pouvoir infestant, la capacité de reproduction, la durée de l'émergence et la longueur de ces JI. Les larves de Galleria mellonella ont été infestées à deux doses: un et 30 JI. Lorsque la durée du stockage augmente, le nombre de nématodes actifs, le pouvoir infestant et la reproduction des JI diminuent, aussi bien à basse qu'à haute température. La durée de la première émergence et la longueur du corps des JI ont été significativement affectées par la durée et la température du stockage, ainsi que par la valeur de l'inoculum. De façon indépendante de cette dernière valeur et de la durée de stockage, le pouvoir infestant la plus élevée et le développement optimal ont été observés lorsque les JI sont stockés à 10 et 15°C.

The effect of storage time and number of spermatozoa per insemination dose on semen characteristics and fertilizing capacity of boar semen diluted with Beltsville Thaw Solution (BTS) extender

1996 ◽  
Vol 62 (3) ◽  
pp. 599-604 ◽  
Author(s):  
C. Alexopoulos ◽  
C. Boscos ◽  
Ph. Saratsis ◽  
C. Saoulidis ◽  
S. Kyriakis
Keyword(s):  
Litter Size ◽  
Storage Time ◽  
Motility Index ◽  
Spermatozoa Motility ◽  
Return Rates ◽  
Semen Collection ◽  
Semen Storage ◽  
Boar Semen ◽  
And Storage ◽  
Zero Time

AbstractSemen collection from three boars was performed twice a week, and doses (100 ml each) of semen diluted with Beltsville Thaw Solution (BTS) extender were prepared containing 1 × 109, 3 × 109 and 5 × 109 spermatozoa. Diluted semen was then stored at 17°C for a maximum of 120 h. Percentage of motile spermatozoa (PMS) and type of spermatozoa motility (TSM) were determined by microscopic examinations performed at 24-h intervals, from zero time (beginning of storage at 17°C) up to 120 h of storage, and a sperm motility index (SMI) calculated. Sows (no. = 360) were divided into nine experimental groups (40 sows per group) and a combination of number of spermatozoa per dose and storage time was used for artificial insemination in each group. All the animals were inseminated twice at 12 and 36 h after detection of standing oestrus by a teaser boar. Return and farrowing data including litter size were recorded. PMS, TSM and SMI decreased significantly (P < 0·01) from 48 h up to 120 h of storage, this being more marked in semen doses of 5 × 109 spermatozoa. When semen stored for up to 24 h was used, no significant differences (P > 0·05) regarding return rates and farrowing rates were observed among the three groups inseminated with semen containing different numbers of spermatozoa per dose. Inseminations with semen doses of 1 × 109 and 3 × 109 spermatozoa stored for 48 to 72 h have significantly lower return rates (P < 0·05) and significantly higher farrowing rates (P < 0·05) than with doses of 5 × 109 spermatozoa. Inseminations with semen stored for more than 72 h gave relatively higher return rates and lower farrowing rates. Semen storage time and number of spermatozoa per dose appeared to reduce litter size only where semen doses of 5 X109 spermatozoa were inseminated after storage for more than 48 h.

Sign in / Sign up

Export Citation Format

Share Document