scholarly journals  Effect of dietary natural supplements on immune response and mineral bioavailability in piglets after weaning

2012 ◽  
Vol 57 (No. 7) ◽  
pp. 332-343 ◽  
Author(s):  
I. Taranu ◽  
D.E. Marin ◽  
A. Untea ◽  
P. Janczyk ◽  
M. Motiu ◽  
...  
Keyword(s):  
Immune Response ◽  
Essential Oils ◽  
Sodium Alginate ◽  
Chlorella Vulgaris ◽  
Plant Extracts ◽  
Cytokine Production ◽  
Feed Additives ◽  
Dietary Sodium ◽  
Weaned Piglets ◽  
Liver Concentration

Plants and plant extracts (PPEs) have gained increasing interest as feed additives and possible replacing antibiotics for pig productions. The effects of dietary Chlorella vulgaris (1%), sodium alginate (0.1%), inulin (1.5%), and a mixture of essential oils (0.04%) supplements on immune response, and bioavailability of some micronutrients (iron − Fe, copper − Cu, manganese − Mn, and zinc − Zn) were investigated in weaned piglets in this study. The results showed that the concentration of IgG was increased in the plasma of pigs fed the PPEs supplemented diets being significantly higher (P < 0.05) in the case of dietary sodium alginate supplementation in comparison to the control (6.00 vs. 4.03 mg/ml). In liver, PPEs, especially inulin and essential oils, were able to modulate the level of cytokine production and mineral retention, resulting in higher liver concentration of IL-1β (125.4 and 88.9%), IL-8 (136.9 and 61.3%), TNF-a (296.6 and 121.6%), and IFN-γ (51.2 and 107.28%), Cu (71.31 ppm), and Fe (192.56 ppm) in comparison to the control. The results of this experiment indicate that natural supplements investigated herein, especially inulin, essential oils, and sodium alginate had the ability to potentiate both the immune function and mineral retention during the initial post weaning period.    

scholarly journals 398 Towards new feeding approaches for optimizing nutritional status, immunity and host-microbiota interactions in neonatal and weaned piglets

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 181-181
Author(s):  
Martin Lessard ◽  
Mylène Blais ◽  
Guylaine Talbot ◽  
J Jacques Matte ◽  
Ann Letellier ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune System ◽  
Intestinal Microbiota ◽  
System Development ◽  
Feed Additives ◽  
Epithelial Cell Line ◽  
Host Immune System ◽  
Gut Health ◽  
Weaned Piglets ◽  
Immune System Development

Abstract Lactation, feeding conditions, microbial interventions and piglet growth in the first few weeks of life have important impact on the intestinal microbiota establishment and immune system development of piglets. Indeed, colostrum and milk contain various bioactive components such as immune factors, antimicrobial peptides and oligosaccharides that contribute to maintain intestinal homeostasis and regulate interactions between microbiota and host immune system. Recent results revealed that low birth weight piglet (LBWP) with poor weight gain during the first two weeks of life develop different intestinal microbiota and immune response profiles compared to high BWP (HBWP) littermates. Consequently, piglets within litters may have different resilience to infections after weaning and benefit from feed additives in a specific manner. A study has been performed to evaluate the potential of bovine colostrum extract (BC) as replacement to plasma proteins for improving gut health and resilience to Salmonella infection in piglets. Results revealed that in weaned piglets fed BC, intestinal microbiota was differently modulated and bacterial dysbiosis induced by Salmonella was restored faster. Moreover, expression of genes involved in innate immunity such as β-defensin-2 and glutathione peroxidase-2 was respectively down- and up-regulated in BC fed piglets. A combination of dietary supplementation with BC, cupper and vitamins A and D has also been tested in LBWP and HBWP, and there is clear evidence that BC in combination with other feed additives promote growth and gut health in both LBWP and HBWP. The porcine intestinal epithelial cell line IPEC-J2 was used to better understand the functional properties of BC. Results indicated that BC improves wound healing, enhances barrier function and modulates the expression of several genes involved in innate immune response. Finally, as microbial intervention, the potential of fecal transplantation to modulate intestinal microbiota and immune system development of piglets is under investigation and will be discussed.

scholarly journals Diet, Immunity, and Microbiota Interactions: An Integrative Analysis of the Intestine Transcriptional Response and Microbiota Modulation in Gilthead Seabream (Sparus aurata) Fed an Essential Oils-Based Functional Diet

2021 ◽  
Vol 12 ◽  
Author(s):  
Joana P. Firmino ◽  
Eva Vallejos-Vidal ◽  
M. Carmen Balebona ◽  
Yuliaxis Ramayo-Caldas ◽  
Isabel M. Cerezo ◽  
...  
Keyword(s):  
Immune Response ◽  
Essential Oils ◽  
Sparus Aurata ◽  
Antioxidant Properties ◽  
Transcriptional Response ◽  
Feed Additives ◽  
Feed Additive ◽  
Gilthead Seabream ◽  
Local Immunity ◽  
The Impact

Essential oils (EOs) are promising alternatives to chemotherapeutics in animal production due to their immunostimulant, antimicrobial, and antioxidant properties, without associated environmental or hazardous side effects. In the present study, the modulation of the transcriptional immune response (microarray analysis) and microbiota [16S Ribosomal RNA (rRNA) sequencing] in the intestine of the euryhaline fish gilthead seabream (Sparus aurata) fed a dietary supplementation of garlic, carvacrol, and thymol EOs was evaluated. The transcriptomic functional analysis showed the regulation of genes related to processes of proteolysis and inflammatory modulation, immunity, transport and secretion, response to cyclic compounds, symbiosis, and RNA metabolism in fish fed the EOs-supplemented diet. Particularly, the activation of leukocytes, such as acidophilic granulocytes, was suggested to be the primary actors of the innate immune response promoted by the tested functional feed additive in the gut. Fish growth performance and gut microbiota alpha diversity indices were not affected, while dietary EOs promoted alterations in bacterial abundances in terms of phylum, class, and genus. Subtle, but significant alterations in microbiota composition, such as the decrease in Bacteroidia and Clostridia classes, were suggested to participate in the modulation of the intestine transcriptional immune profile observed in fish fed the EOs diet. Moreover, regarding microbiota functionality, increased bacterial sequences associated with glutathione and lipid metabolisms, among others, detected in fish fed the EOs supported the metabolic alterations suggested to potentially affect the observed immune-related transcriptional response. The overall results indicated that the tested dietary EOs may promote intestinal local immunity through the impact of the EOs on the host-microbial co-metabolism and consequent regulation of significant biological processes, evidencing the crosstalk between gut and microbiota in the inflammatory regulation upon administration of immunostimulant feed additives.

In vitro screening of selected feed additives, plant essential oils and plant extracts for rumen methane mitigation

2013 ◽  
Vol 94 (6) ◽  
pp. 1191-1196 ◽  
Author(s):  
Zoey Durmic ◽  
Peter J Moate ◽  
Richard Eckard ◽  
Dean K Revell ◽  
Richard Williams ◽  
...  
Keyword(s):  
Essential Oils ◽  
Plant Extracts ◽  
Feed Additives ◽  
In Vitro Screening ◽  
Methane Mitigation ◽  
Plant Essential Oils

scholarly journals Effectiveness of Two Plant-Based In-Feed Additives against an Escherichia coli F4 Oral Challenge in Weaned Piglets

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2024
Author(s):  
Daniel Montoya ◽  
Matilde D’Angelo ◽  
Susana M. Martín-Orúe ◽  
Agustina Rodríguez-Sorrento ◽  
Mireia Saladrigas-García ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Immune Response ◽  
Control Diet ◽  
Clinical Signs ◽  
Feed Additives ◽  
Intestinal Morphology ◽  
Enteric Pathogens ◽  
Weaned Piglets ◽  
Microbial Analysis

This study evaluates the efficacy of two plant-based feed supplementations to fight colibacillosis in weanlings. A total of 96 piglets (32 pens) were assigned to four diets: a control diet (T1) or supplemented with ZnO (2500 ppm Zn) (T2) or two different plant supplements, T3 (1 kg/t; based on essential oils) and T4 (T3 + 1.5 kg/t based on non-volatile compounds). After one week, animals were challenged with ETEC F4, and 8 days after, one animal per pen was euthanized. Performance, clinical signs, microbial analysis, inflammatory response, intestinal morphology, and ileal gene expression were assessed. ZnO improved daily gains 4 days after challenge, T3 and T4 showing intermediate values (96, 249, 170, and 157 g/d for T1, T2, T3, and T4, p = 0.035). Fecal lactobacilli were higher with T3 and T4 compared to ZnO (7.55, 6.26, 8.71, and 8.27 cfu/gFM; p = 0.0007) and T3 increased the lactobacilli/coliforms ratio (p = 0.002). T4 was associated with lower levels of Pig-MAP (p = 0.07) and increases in villus/crypt ratio (1.49, 1.90, 1.73, and 1.84; p = 0.009). Moreover, T4 was associated with an upregulation of the REG3G gene (p = 0.013; pFDR = 0.228) involved in the immune response induced by enteric pathogens. In conclusion, both plant supplements enhanced animal response in front of an ETEC F4 challenge probably based on different modes of action.

Effect of plant extracts on methanogenesis and microbial profile of the rumen of buffalo: a brief overview

2008 ◽  
Vol 48 (2) ◽  
pp. 175 ◽  
Author(s):  
D. N. Kamra ◽  
A. K. Patra ◽  
P. N. Chatterjee ◽  
Ravindra Kumar ◽  
Neeta Agarwal ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Essential Oils ◽  
Plant Extracts ◽  
Mangifera Indica ◽  
Plant Secondary Metabolites ◽  
Feed Additives ◽  
Production Test ◽  
Syzygium Aromaticum ◽  
Ciliate Protozoa

Plants rich in secondary metabolites (saponins, tannins, essential oils, etc.) have antimicrobial activity which can be exploited for selective inhibition of a particular group of microbes in the rumen. We have screened a large number of plant extracts for their potential to inhibit methanogenesis and ciliate protozoa in an in vitro gas production test using buffalo rumen liquor as the inoculum. Out of 93 plant extracts tested, 11 inhibited in vitro methanogenesis to the extent of 25–50% and nine plant extracts inhibited methanogenesis more than 50%. Among 20 extracts exhibiting antimethanogenic activity, nine were ethanol extracts, 10 were methanol extracts and only one was a water extract. Some of these plant extracts inhibited ciliate protozoa as tested by microscopic examination and 14C-labelled radioisotopic technique, but the protozoa inhibition was not correlated with methane inhibition, indicating that the methanogens sensitive to plant secondary metabolites may or may not be having any symbiotic relationship with ciliate protozoa. Methane inhibition was accompanied by a drastic fall in the number of methanogens as determined by real time PCR. Plants that appeared to have some potential as feed additives to control methanogenesis by the ruminants are: (i) seed pulp of Sapindus mukorossi (rich in saponins) and Terminalia chebula (rich in tannins); (ii) leaves of Populus deltoides, Mangifera indica and Psidium guajava (rich in tannins and essential oils); and (iii) flower buds of Syzygium aromaticum and bulb of Allium sativum (rich in essential oils). Some of the plants reported in literature exhibiting antimethanogenic activity include Equisetum arvense, Lotus corniculatus, Rheum palmatum, Salvia officinalis, Sapindus saponaria, Uncaria gambir and Yucca schidigera.

PSVI-16 The Association of Benzoic Acid and Essential Oils Eo Reduces the Inflammatory Response of Weaned Piglets and the Diarrhea Incidence in the Nursery Phase

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 202-203
Author(s):  
Sudario Roberto Silva Junior ◽  
Maíra Resende ◽  
Rhuan F Chaves ◽  
Jéssica Aparecida Barbosa ◽  
Iana I M Ferreira ◽  
...  
Keyword(s):  
Body Weight ◽  
Inflammatory Response ◽  
Benzoic Acid ◽  
Essential Oils ◽  
Growth Performance ◽  
Block Design ◽  
Basal Diet ◽  
Weaned Piglets ◽  
Total Period ◽  
Diarrhea Incidence

Abstract Benzoic acid (BA) and essential oils (EO) can minimize growth performance losses due to the removal of antibiotics and change the intestinal health of weaned piglets. The objective of this study was to evaluate the effects of BA and EO on inflammatory response, diarrhea incidence, and growth performance of the nursery phase. One hundred and twenty barrows were weaned at 23 days (6.40 ± 0.53 kg) and assigned into 3 treatments (10 replicates) in randomized block design: basal diet without additives (NC), basal diet with 200 ppm of colistin sulphate (PC), and association of 0,3% benzoic acid and essential oil (BA+EO). The feed intake and body weight were recorded at 0 and 42 days. The feces were assessed daily (per animal) and graded as normal feces (no diarrhea) or liquid or pasty stools (presence of diarrhea). On days 1, 3, and 9, blood samples were collected (5 replicates) for white blood cells (WBC) counts. Growth performance was analyzed by MIXED procedure (SAS, 2009) and the Tukey test was used to compare the means (P < 0.050). The WBC counts were analyzed by repeated-measures analysis of variance, by MIXED procedure. Diarrhea incidence was analyzed by GENMOD procedure (SAS, 2009). The BA+EO treatment showed a similar body weight (P = 0.014) and average daily gain (P = 0.012) than the PC group and lower feed conversion ratio (P = 0.037) compared to the NC group. The pigs of the BA+EO treatment had the lowest diarrhea incidence during the total period (P < 0.001). The supplementation with BA+EO or antibiotics reduced the counts of total WBC (P = 0.008) and neutrophils (P = 0.003). In conclusion, supplementation with BA+EO reduces the inflammatory response and the incidence of diarrhea in the nursery phase, that may be related to the improvement in the FCR.

scholarly journals Clostridium Butyricum ZJU-F1 Benefits the Intestinal Barrier Function and Immune Response Associated with Its Modulation of Gut Microbiota in Weaned Piglets

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 527
Author(s):  
Jie Fu ◽  
Tenghao Wang ◽  
Xiao Xiao ◽  
Yuanzhi Cheng ◽  
Fengqin Wang ◽  
...  
Keyword(s):  
Immune Response ◽  
Bacillus Licheniformis ◽  
Barrier Function ◽  
Intestinal Barrier ◽  
Clostridium Butyricum ◽  
Intestinal Barrier Function ◽  
Weaned Piglets ◽  
Total Acid ◽  
Junction Protein ◽  
Mucin Expression

This study investigated the effects of dietary C. butyricum ZJU-F1 on the apparent digestibility of nutrients, intestinal barrier function, immune response, and microflora of weaned piglets, with the aim of providing a theoretical basis for the application of Clostridium butyricum as an alternative to antibiotics in weaned piglets. A total of 120 weanling piglets were randomly divided into four treatment groups, in which piglets were fed a basal diet supplemented with antibiotics (CON), Bacillus licheniformis (BL), Clostridium butyricum ZJU-F1 (CB), or Clostridium butyricum and Bacillus licheniformis (CB-BL), respectively. The results showed that CB and CB-BL treatment increased the intestinal digestibility of nutrients, decreased intestinal permeability, and increased intestinal tight junction protein and mucin expression, thus maintaining the integrity of the intestinal epithelial barrier. CB and CB-BL, as exogenous probiotics, were also found to stimulate the immune response of weaned piglets and improve the expression of antimicrobial peptides in the ileum. In addition, dietary CB and CB-BL increased the proportion of Lactobacillus. The levels of butyric acid, propionic acid, acetic acid, and total acid were significantly increased in the ceca of piglets fed CB and CB-BL. Furthermore, we validated the effects of C. butyricum ZJU-F1 on the intestinal barrier function and immune response in vitro and found C. butyricum ZJU-F1 improved intestinal function and enhanced the TLR-2-MyD88-NF-κB signaling.

Development and characterization of sodium alginate based active edible films incorporated with essential oils of some medicinal plants

2020 ◽  
Vol 145 ◽  
pp. 124-132 ◽  
Author(s):  
Zineb Mahcene ◽  
Aminata Khelil ◽  
Sara Hasni ◽  
Perihan Kubra Akman ◽  
Fatih Bozkurt ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Essential Oils ◽  
Sodium Alginate ◽  
Edible Films

All-natural composite wound dressing films of essential oils encapsulated in sodium alginate with antimicrobial properties

2014 ◽  
Vol 463 (2) ◽  
pp. 137-145 ◽  
Author(s):  
Ioannis Liakos ◽  
Loris Rizzello ◽  
David J. Scurr ◽  
Pier Paolo Pompa ◽  
Ilker S. Bayer ◽  
...  
Keyword(s):  
Essential Oils ◽  
Sodium Alginate ◽  
Wound Dressing ◽  
Antimicrobial Properties ◽  
Natural Composite
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