scholarly journals Antioxidant activities of peptide fractions derived from freshwater mussel protein using ultrasound-assisted enzymatic hydrolysis

2017 ◽  
Vol 35 (No. 4) ◽  
pp. 328-338 ◽  
Author(s):  
Dong Zhouyong ◽  
Tian Gang ◽  
Xu Zhaogang ◽  
Li Mingyue ◽  
Xu Min ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Amino Acid ◽  
Antioxidant Activities ◽  
Freshwater Mussel ◽  
Acid Oxidation ◽  
Linoleic Acid Oxidation ◽  
Chelating Activity ◽  
Ultrasound Assisted ◽  
Peptide Fractions

The freshwater mussel protein was hydrolysed using ultrasound-assisted enzymolysis. Ultrasound-assisted freshwater mussel protein hydrolysates (UPH) were divided into four fractions (&gt; 10, 6–10, 3–6, and &lt; 3 kDa) using ultrafiltration, and the fraction with the highest antioxidant activity was further subdivided into four fractions (F<sub>1</sub>–F<sub>4</sub>) using gel chromatography. The amino acid compositions and antioxidant activities (DPPH, hydroxyl and superoxide radical scavenging activities, reducing power, ferrous ion chelating activity, and inhibition of linoleic acid oxidation) of peptide fractions were investigated. The results showed that the antioxidant activity of the &lt; 3 kDa fraction was significantly higher than that of UPH, &gt; 10, 6–10, and 3–6 kDa fractions. The antioxidant activity of F<sub>2</sub> was again higher compared with the &lt; 3 kDa fraction and higher than that of F<sub>1</sub>, F<sub>3</sub>, and F<sub>4</sub>. Amino acid analysis showed that the antioxidant activities (except for chelating activity) of peptides increased with increasing hydrophobic amino acid content. The &lt; 3 kDa and F<sub>2</sub> fractions exhibited strong inhibition of linoleic acid oxidation, their effects being even better than that of ascorbic acid (Vc) and l-glutathione (GSH). Therefore, these peptide fractions from freshwater mussel may be a potential natural antioxidant that could be added to various foods.

scholarly journals A Novel Insight into Screening for Antioxidant Peptides from Hazelnut Protein: Based on the Properties of Amino Acid Residues

Antioxidants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 127
Author(s):  
Chenshan Shi ◽  
Miaomiao Liu ◽  
Hongfei Zhao ◽  
Zhaolin Lv ◽  
Lisong Liang ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Amino Acid ◽  
Hazelnut Oil ◽  
Acid Oxidation ◽  
Amino Acid Residues ◽  
Antioxidant Peptides ◽  
Water Emulsion ◽  
Linoleic Acid Oxidation ◽  
Grand Average ◽  
Novel Approach

This study used the properties of amino acid residues to screen antioxidant peptides from hazelnut protein. It was confirmed that the type and position of amino acid residues, grand average of hydropathy, and molecular weight of a peptide could be comprehensively applied to obtain desirable antioxidants after analyzing the information of synthesized dipeptides and BIOPEP database. As a result, six peptides, FSEY, QIESW, SEGFEW, IDLGTTY, GEGFFEM, and NLNQCQRYM were identified from hazelnut protein hydrolysates with higher antioxidant capacity than reduced Glutathione (GSH) against linoleic acid oxidation. The peptides having Tyr residue at C-terminal were found to prohibit the oxidation of linoleic acid better than others. Among them, peptide FSEY inhibited the rancidity of hazelnut oil very well in an oil-in-water emulsion. Additionally, quantum chemical parameters proved Tyr-residue to act as the active site of FSEY are responsible for its antioxidation. This is the first presentation of a novel approach to excavating desired antioxidant peptides against lipid oxidation from hazelnut protein via the properties of amino acid residues.

scholarly journals Assessment of antioxidant properties of membrane ultrafiltration peptides from mungbean meal protein hydrolysates

2018 ◽  
Author(s):  
Chanikan Sonklin ◽  
Natta Laohakunjit ◽  
Orapin Kerdchoechuen
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Amino Acid ◽  
Metal Ion ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Peptide Fractions

Background Bioactive peptides can prevent damage associated with oxidative stress in the human body when consumed regularly. Recently year, peptides have attracted immense interest because of their beneficial functional properties, safety and little or no side effects when used at high concentration. Most antioxidant peptide has small size less than 1 kDa and contains high proportion of hydrophobic amino acid. Particularly Tyr, Leu, Ala, Ile, Val, Lys, Phe, Cys, Met and His exhibited high antioxidant activity. Mungbean protein contain high abundance of protein and hydrophobic amino acid contents, investigating its bioactivity is an important aspect of adding value to this by-product obtained from a growing industry. Therefore, the objectives of this study were to optimize the conditions used to generate MMPH with antioxidant activity form bromelain and to investigate the antioxidant activities of each molecular weight peptide fraction. Methods Response Surface Methodology (RSM) was used for screening the optimal conditions to produce Mungbean meal protein hydrolysate (MMPH). After that optimal MMPH was fractionated using ultrafiltration membranes with different molecular weight (MW) distribution. Crude-MMPH and four peptide fractions were investigated for five antioxidant activities: DPPH scavenging activity, Hydroxyl scavenging activity, Superoxide scavenging activity, Ferric reducing antioxidant power and metal ion chelation activity. Results The optimal condition of crude-MMPH production was 12 % (w/w) of bromelain and hydrolysis time for 12 h. The EC50 of DPPH was the highest for the F4 peptide fraction (MW<1 kDa) at 0.5320 mg/mL. Metal ion chelating activity was generally weak, except for the F4 that had a value of 43.94% at a protein concentration of 5 mg/mL. The F4 also exhibited high hydroxyl and superoxide radical scavenging activities (54 and 65.1%), but poor activity for ferric reducing antioxidant power (0.102 mM Fe2+/mg protein) compared to other peptide fractions and crude-MMPH. Molecular weight and amino acid were the main factors that determined the antioxidant activities of these peptide fractions. Results show that F4 have high antioxidant potentials. Discussion The lowest MW Fraction (less than 1 kDa) showed the highest DPPH activity, superoxide-, hydroxyl-scavenging activity and metal chelation activity. On the other hand, this fraction had poor ferric reducing power. This showed that low molecular weight has an important effect on antioxidant activities. According to the mechanism of the reaction, the potential of antioxidant activity was divided into two main groups: hydrogen atom transfer (HAT) and single electron transfer (SET). Therefore, this finding suggests that the antioxidant mechanism of peptides obtained mungbean could react with many species of free radicals by multiple mechanisms. Mungbean meal peptide can be developed into multiple functional foods which possess both antioxidant properties and aroma/taste.

Antioxidant activity of egg-yolk protein hydrolysates in a linoleic acid oxidation system

2004 ◽  
Vol 86 (1) ◽  
pp. 99-103 ◽  
Author(s):  
Senji Sakanaka ◽  
Yumi Tachibana ◽  
Noriyuki Ishihara ◽  
Lekh Raj Juneja
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Egg Yolk ◽  
Protein Hydrolysates ◽  
Yolk Protein ◽  
Acid Oxidation ◽  
Linoleic Acid Oxidation ◽  
Oxidation System

Antioxidant Activity of Melanoidins from Different Sugar/Amino Acid Model Systems: Influence of the Enantiomer Type

2009 ◽  
Vol 15 (3) ◽  
pp. 291-297 ◽  
Author(s):  
Ji-Sang Kim ◽  
Young-Soon Lee
Keyword(s):  
Antioxidant Activity ◽  
Amino Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Soluble ◽  
Model Systems ◽  
Ferrous Ion ◽  
In Vitro Assays ◽  
Antioxidant Power ◽  
Chelating Activity

The effect of amino acid enantiomers on the antioxidant activity of melanoidins was examined. For this purpose, antioxidant activities were evaluated on the basis of ferric reducing/antioxidant power (FRAP) and free radical scavenging activity including 1, 1-diphenyl-2-picryl-hydrazil (DPPH) and 2, 2'-azinobis (3-ethylbenothiazoline-6-sulfonic acid), diammonium salt (ABTS) and ferrous ion chelating activity. Ethylene diamine tetraacetate and trolox, a water-soluble analog of tocopherol, were used as the reference antioxidant compounds. Melanoidins formed from D-isomers were found to be effective antioxidants in different in vitro assays with regard to the ferrous ion chelating activity, ABTS and DPPH radical scavenging activities, and FRAP. In particular, the antioxidant activities of melanoidins formed from fructose systems were higher than those of melanoidins formed from glucose systems. In addition, the results showed that melanoidins formed from D-isomers had antioxidant activities, similar to those of melanoidins formed from L-isomers.

scholarly journals Assessment of antioxidant properties of membrane ultrafiltration peptides from mungbean meal protein hydrolysates

2018 ◽  
Author(s):  
Chanikan Sonklin ◽  
Natta Laohakunjit ◽  
Orapin Kerdchoechuen
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Amino Acid ◽  
Metal Ion ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Peptide Fractions

Background Bioactive peptides can prevent damage associated with oxidative stress in the human body when consumed regularly. Recently year, peptides have attracted immense interest because of their beneficial functional properties, safety and little or no side effects when used at high concentration. Most antioxidant peptide has small size less than 1 kDa and contains high proportion of hydrophobic amino acid. Particularly Tyr, Leu, Ala, Ile, Val, Lys, Phe, Cys, Met and His exhibited high antioxidant activity. Mungbean protein contain high abundance of protein and hydrophobic amino acid contents, investigating its bioactivity is an important aspect of adding value to this by-product obtained from a growing industry. Therefore, the objectives of this study were to optimize the conditions used to generate MMPH with antioxidant activity form bromelain and to investigate the antioxidant activities of each molecular weight peptide fraction. Methods Response Surface Methodology (RSM) was used for screening the optimal conditions to produce Mungbean meal protein hydrolysate (MMPH). After that optimal MMPH was fractionated using ultrafiltration membranes with different molecular weight (MW) distribution. Crude-MMPH and four peptide fractions were investigated for five antioxidant activities: DPPH scavenging activity, Hydroxyl scavenging activity, Superoxide scavenging activity, Ferric reducing antioxidant power and metal ion chelation activity. Results The optimal condition of crude-MMPH production was 12 % (w/w) of bromelain and hydrolysis time for 12 h. The EC50 of DPPH was the highest for the F4 peptide fraction (MW<1 kDa) at 0.5320 mg/mL. Metal ion chelating activity was generally weak, except for the F4 that had a value of 43.94% at a protein concentration of 5 mg/mL. The F4 also exhibited high hydroxyl and superoxide radical scavenging activities (54 and 65.1%), but poor activity for ferric reducing antioxidant power (0.102 mM Fe2+/mg protein) compared to other peptide fractions and crude-MMPH. Molecular weight and amino acid were the main factors that determined the antioxidant activities of these peptide fractions. Results show that F4 have high antioxidant potentials. Discussion The lowest MW Fraction (less than 1 kDa) showed the highest DPPH activity, superoxide-, hydroxyl-scavenging activity and metal chelation activity. On the other hand, this fraction had poor ferric reducing power. This showed that low molecular weight has an important effect on antioxidant activities. According to the mechanism of the reaction, the potential of antioxidant activity was divided into two main groups: hydrogen atom transfer (HAT) and single electron transfer (SET). Therefore, this finding suggests that the antioxidant mechanism of peptides obtained mungbean could react with many species of free radicals by multiple mechanisms. Mungbean meal peptide can be developed into multiple functional foods which possess both antioxidant properties and aroma/taste.

scholarly journals AKTIVITAS ANTIOKSIDAN EKSTRAK BIJI DUWET (Syzygium cumini Linn.) PADA PEROKSIDASI LIPIDA SECARA IN VITRO

2016 ◽  
Vol 36 (01) ◽  
pp. 30 ◽  
Author(s):  
Rohadi Rohadi ◽  
Sri Rahadjo ◽  
Lip Izul Falah ◽  
Umar Santoso
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Reducing Power ◽  
Seed Extract ◽  
Total Phenolic ◽  
Acid Oxidation ◽  
Syzygium Cumini ◽  
Antioxidant Power ◽  
Linoleic Acid Oxidation

All parts of Syzygium cumini Linn. (duwet) were widely used for medicinal plant in the treatment of various deseases.The seed extract was used to lower blood glucose. Cumini's seed had higher phenolic fractions than others. It were prepared by extracted of duwet seed "Genthong" varieties, using various extractants such as 85% ethyl acetate, 50% methanol and 50% ethanol. Duwet seed extract collected then was determined of phenolics compound and antioxidant activity assayed by measuring 2,2-diphenyl-1-picrylhydrazylradical (DPPH) scavenging activity, reducing Ferric ion (Fe3+) power and inhibition of linoleic acid oxidation. The objective was to select one of the extractant which gave highest yield and polyphenolic content and its stronger antioxidant activity. Extractant 50% methanol gave highest the extract yields was 16.29 % (db.) and phenolics compounds of extract was composed: total phenolic 45.99±0.25 g-GAE/100 g-extract; total flavonoid 2.28±0.07 g-QE/100 g-extract and total tannin 26.9±0.07 g-TAE/100 g-extract. All of extract exhibited strong on behalf of RSA-DPPH assay 87-95% (100  g-mL-l) and reducing power, nevertheless in inhibition of linoleic acid oxidation was moderate 49-55% (400  g-mL-l).Keywords: Syzygium cumini Linn, seed, antioxidant, extraction ABSTRAKTanaman duwet (Syzygium cumini Linn.) pada semua kompartemennya dimanfaatkan masyarakat untuk pengobatansuatu penyakit. Ekstrak bijinya dimanfaatkan untuk penurun gula darah. Bijinya merupakan bagian tanaman yang kaya senyawa polifenol. Fraksi kaya senyawa fenolik dipreparasi dengan mengekstraksi biji duwet varietas "Genthong", dengan tiga jenis ekstraktan; etil acetat 85%, metanol 50% dan etanol 50%. Ekstrak biji duwet (EBD) yang diperoleh dianalisis kelompok senyawa fenolik dan aktivitas antioksidan menggunakan metode uji penangkapan radikal DPPH (2,2-diphenil 1-picrylhydrazyl), uji reduksi ion Feri (ferric reduction antioxidant power-FRAP) dan uji penghambatan peroksidasi asam lemak linoleat. Tujuan penelitian adalah memilih satu dari tiga ekstraktan yang menghasilkaan ekstrak, dengan yield dan kadar senyawa polifenolik terbesar serta sifat antioksidatif terkuat. Yield dengan ekstraktan Met-OH-50%, sebesar 16,29% (db), senyawa fenoliknya sebesar 45,99 ±0,25 g-GAE/100 g-EBD; 2,28±0,07 g-QE/100 g-EBD dan 26,9±0,07 g-TAE/100 g-EBD. Ketiga ekstrak kuat dalam uji penangkapan radikal DPPH antara 87-95 % (100 g-mL-l) dan uji reduksi ion Feri (Fe3+), moderat pada uji penghambatan peroksidasi lipid, 49-52 % pada 400  g- mL-l.Kata kunci: Biji duwet (Syzygium cumini Linn.), antioksidan, ekstraksi

Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media

1989 ◽  
Vol 66 (6) ◽  
pp. 792-799 ◽  
Author(s):  
R. S. Farag ◽  
A. Z. M. A. Badei ◽  
F. M. Hewedi ◽  
G. S. A. El-Baroty
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Essential Oils ◽  
Aqueous Media ◽  
Acid Oxidation ◽  
Linoleic Acid Oxidation

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

scholarly journals The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2739
Author(s):  
Liza Devita ◽  
Hanifah Nuryani Lioe ◽  
Mala Nurilmala ◽  
Maggy T. Suhartono
Keyword(s):  
Antioxidant Activity ◽  
In Silico ◽  
Antioxidant Activities ◽  
In Silico Analysis ◽  
Weight Fraction ◽  
Amino Acid Sequences ◽  
Skin Collagen ◽  
Dpph Method ◽  
Peptide Fractions

The hydrolysates and peptide fractions of bigeye tuna (Thunnus obesus) skin collagen have been successfully studied. The hydrolysates (HPA, HPN, HPS, HBA, HBN, HBS) were the result of the hydrolysis of collagen using alcalase, neutrase, and savinase. The peptide fractions (PPA, PPN, PPS, PBA, PBN, PBS) were the fractions obtained following ultrafiltration of the hydrolysates. The antioxidant activities of the hydrolysates and peptide fractions were studied using the DPPH method. The effects of collagen types, enzymes, and molecular sizes on the antioxidant activities were analyzed using profile plots analysis. The amino acid sequences of the peptides in the fraction with the highest antioxidant activity were analyzed using LC-MS/MS. Finally, their bioactivity and characteristics were studied using in silico analysis. The hydrolysates and peptide fractions provided antioxidant activity (6.17–135.40 µmol AAE/g protein). The lower molecular weight fraction had higher antioxidant activity. Collagen from pepsin treatment produced higher activity than that of bromelain treatment. The fraction from collagen hydrolysates by savinase treatment had the highest activity compared to neutrase and alcalase treatments. The peptides in the PBN and PPS fractions of <3 kDa had antidiabetic, antihypertensive and antioxidant activities. In conclusion, they have the potential to be used in food and health applications.

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