scholarly journals Evaluation of protein fractions as biochemical markers for identification of spelt wheat cultivars (Triticum spelta L.)

2011 ◽  
Vol 49 (No. 3) ◽  
pp. 99-105 ◽  
Author(s):  
V. Dvořáček ◽  
V. Čurn
Keyword(s):  
Common Wheat ◽  
Biochemical Markers ◽  
Digital Image Analysis ◽  
Wheat Cultivar ◽  
Protein Fractions ◽  
Wheat Cultivars ◽  
Triticum Spelta ◽  
Spelt Wheat ◽  
Sds Page ◽  
Polymorphism Level

Four protein fractions: 1 – albumins and globulins, 2 – gliadins, 3 – glutenins (extracted in NaOH), 4 – glutenins (extracted in SDS) separated by SDS-PAGE were used as biochemical markers for evaluation of polymorphism level in three spelt wheat cultivars – Hercule, Altgold and Rouquin, three new–breeders’ spelt lines – H92.27, H92.28 and M92.20 (originated from hybridisation between spelt and common wheat) and reference common wheat cultivar Brea. Electrophoretic phenotypes and zymograms were evaluated by means of digital image analysis and Nei and Li coefficient of similarity was used to evaluate the relation of analysed genotypes. Entire evaluation of all four-marker systems showed differences between common wheat cultivarBreaand spelt cultivars and spelt breeders’ lines. Also significant differences between old spelt cultivars (Hercule, Altgold and Rouquin) and new spelt breeders’ lines were found. The reality of the mutual passing of protein fractions (gliadins and glutenins), based on Osborne extraction was confirmed. In this sense it is necessary to see both fractions as dynamic overlapping structures.

scholarly journals Improved Method for Reliable HMW-GS Identification by RP-HPLC and SDS-PAGE in Common Wheat Cultivars

Molecules ◽  
2017 ◽  
Vol 22 (7) ◽  
pp. 1055 ◽  
Author(s):  
You-Ran Jang ◽  
Hye-Rang Beom ◽  
Susan Altenbach ◽  
Min-Ki Lee ◽  
Sun-Hyung Lim ◽  
...  
Keyword(s):  
Common Wheat ◽  
Wheat Cultivars ◽  
Improved Method ◽  
Rp Hplc ◽  
Sds Page

scholarly journals Genetic diversity of Polish cultivars of common wheat (Triticum aestivum L.) based on molecular and protein markers

2020 ◽  
Vol 48 (2) ◽  
pp. 535-548
Author(s):  
Ewa FILIP ◽  
Aleksandra STROŃSKA ◽  
Magdalena SZENEJKO ◽  
Waldemar PLUTA
Keyword(s):  
Genetic Diversity ◽  
Common Wheat ◽  
Similarity Index ◽  
Principal Component ◽  
Shannon Index ◽  
Qualitative Assessment ◽  
Wheat Cultivars ◽  
Protein Markers ◽  
Binary Matrix ◽  
Sds Page

Knowledge of the composition subunits of proteins glutenins makes it much easier to determine the quality and suitability of the final product obtained from gluten. It is important to remember that not only molecular processes model gluten protein polymorphism. In this study, the genetic diversity of Polish common wheat cultivars was examined at the level of DNA and glutenin’s proteins HMW-GS. The SDS-PAGE and RAPD bands were evaluated in binary matrix, which was the basis for further analysis of results, using appropriate measures of variability: Q-Cochran  test (Cochran, 1950), p < 0.05; I-Shannon index; Si-Similarity index; PIC-Polymorphism Information Content; (PCA-Principal component analysis). Both types of markers proved to be useful in the overall assessment of genetic variability between tested of common wheat cultivars. The general genetic diversity indicates that good candidates with unique composition of HMW-GS subunits were selected among the examined cultivars and three OPA-02, OPA-03, OPB-08 primers with the highest power differentiating for the studied genotypes were selected. Results of the research revealed the potentials of RAPD and SDS-PAGE technique in determining genetical diversity and make a suitable qualitative assessment of common wheat cultivars.

scholarly journals Reaction of wheat, alternative wheat and triticale cultivars to common bunt

2010 ◽  
Vol 46 (No. 1) ◽  
pp. 14-20 ◽  
Author(s):  
V. Dumalasová ◽  
P. Bartoš
Keyword(s):  
Czech Republic ◽  
Bread Wheat ◽  
Wheat Cultivar ◽  
Field Trials ◽  
Emmer Wheat ◽  
Wheat Cultivars ◽  
Common Bunt ◽  
The Czech Republic ◽  
Spelt Wheat ◽  
Winter Wheat Cultivars

&nbsp;Seventeen winter wheat cultivars registered in the Czech Republic were tested for reaction to common bunt in 2&ndash;3 year field trials. Bunt infection of resistant checks Globus and Bill varied between 4.1% and 10.6%; the highest infection in cv. Pitbull reached 85.9%. Of the recently registered cultivars Nikol has a relatively low bunt incidence (26.9%). In addition to bread wheat seventeen triticale, seven durum wheat cultivars, two spelt wheat cultivars and one emmer wheat cultivar were tested in the field and some of them also in the greenhouse. Bunt infection of durum wheats was lower than that of bread wheat cultivars. All seventeen tested triticale cultivars were resistant. The reaction of emmer wheat cultivar and spelt wheat cultivars to common bunt was lower than that of susceptible bread wheat checks.

Root rot severity and fungal populations in spring common, durum and spelt wheat, and Kamut grown under organic management in western Canada

2014 ◽  
Vol 94 (5) ◽  
pp. 937-946 ◽  
Author(s):  
M. R. Fernandez ◽  
S. L. Fox ◽  
P. Hucl ◽  
A. K. Singh ◽  
F. C. Stevenson
Keyword(s):  
Durum Wheat ◽  
Common Wheat ◽  
Root Rot ◽  
Western Canada ◽  
Wheat Cultivars ◽  
Fusarium Spp ◽  
Organic Management ◽  
Spelt Wheat ◽  
Fungal Populations ◽  
Relative Prevalence

Fernandez, M. R., Fox, S. L., Hucl, P., Singh, A. K. and Stevenson, F. C. 2014. Root rot severity and fungal populations in spring common, durum and spelt wheat, and Kamut grown under organic management in western Canada. Can. J. Plant Sci. 94: 937–946. A 3-yr field study (2010 to 2012) was conducted in the Brown soil zone of southwest Saskatchewan to determine the reactions of common, durum and spelt wheat cultivars currently registered in western Canada, and of Kamut wheat, to common root rot (CRR) under organic management. The genotypes selected for this study are often grown by organic producers in this region. Over the 3 yr of this study, Cochliobolus sativus, the main causal agent of CRR, was the fungus most frequently isolated from discoloured subcrown internodes, followed by Fusarium spp. The latter constituted an overall total of over 19% of all isolations and consisted of at least 12 different species, the most frequently detected of which were F. equiseti, F. avenaceum, F. acuminatum and F. oxysporum. The relative prevalence of the most commonly isolated genera/species agrees most closely with previous studies conducted under organic management. In general, C. sativus was less common in Kamut than in durum and spelt wheat, and it was more frequently isolated from durum than common wheat. In contrast, there were few differences in the isolation of Fusarium spp. among wheat species. Their isolation was greater for common wheat and Kamut than for durum wheat. For all 3 yr, the greatest mean CRR severity was observed in spelt wheat, followed by durum wheat and Kamut, with common wheat having the lowest average severity. For individual cultivars, the durum wheat AC Avonlea, Kyle and Transcend had the greatest CRR severity of all cultivars in this species and CDC Verona the lowest. Common wheat cultivars AC Elsa, CDC Kernen and Red Fife had the greatest CRR severity and Superb and Unity the lowest severity within their species. Under organic conditions, avoiding growing cultivars with high susceptibility to CRR is recommended given the expected presence of this disease in most fields and environments.

scholarly journals Identification of Glutenin Markers in Cultivars of three Wheat Species

2011 ◽  
Vol 39 (No. 2) ◽  
pp. 51-57 ◽  
Author(s):  
M. Starovičová ◽  
Z. Gálová ◽  
H. Knoblochová
Keyword(s):  
Winter Wheat ◽  
Triticum Aestivum L ◽  
Wheat Cultivars ◽  
Triticum Spelta ◽  
Spelt Wheat ◽  
Gluten Quality ◽  
Electrophoretic Spectra ◽  
Winter Wheat Cultivars ◽  
European Winter Wheat ◽  
Triticum Durum Desf

In a collection of 41 Slovak and European winter wheat cultivars (Triticum aestivum L.), 5 spelt wheat cultivars (Triticum spelta L.) and 3 durum wheat cultivars (Triticum durum DESF.) we investigated the qualitative composition of high-molecular-weight glutenin subunits (HMW-GS), the Gluten quality (GQ) score and the presence of the secale block 1B3. The highest frequency of the HMW-GS 0, 7 + 9 and 5 + 10 was found among the winter wheat cultivars. The highest GQ score of 10 was found in the cultivars SK-30 and FD-92017/1. A GQ score of 9 was found in the cultivars Astella, Ilona, Velta and MV-06-95. The HMW-GS 0 and 7 + 8, associated with a GQ of 4, were found in T. durum. The electrophoretic spectra of the spelt wheat cultivars were not homogeneous and represented 4 different lines. &nbsp;

Effect of algae Ecklonia maxima extract (Kelpak SL) on yields of common wheat, durum wheat and spelt wheat

2019 ◽  
Vol 74 (1) ◽  
pp. 5-14
Author(s):  
GRZEGORZ SZUMIŁO ◽  
LESZEK RACHOŃ ◽  
BARBARA KROCHMAL-MARCZAK
Keyword(s):  
Triticum Aestivum ◽  
Durum Wheat ◽  
Common Wheat ◽  
Yield Components ◽  
Grain Quality ◽  
Foliar Application ◽  
Control Treatment ◽  
Favourable Effect ◽  
Ecklonia Maxima ◽  
Spelt Wheat

The 3-year experiment was concerned with the response of spring forms of common wheat (Triticum aestivum L. subsp. aestivum), durum wheat (Triticum durum Desf.) and spelt wheat (Triticum aestivum subsp. spelta L. em. Thell.) to the foliar application of a plant growth stimulant (extract from marine algae Ecklonia maxima), with the commercial name of Kelpak SL (GS), as compared to control treatment (C). The following parameters were analysed: yield of grain, yield components (number of ears, weight of 1000 kernels, number and weight of kernels per ear) and physical indicators of grain quality (test weight, uniformity and vitreosity of grain). The study showed that the level of yielding and the yield components were related primarily with the wheat genotype, but they depended also on the agro-climatic conditions and on the algae extract and control experimental treatments. The application of algae extract, compared to the control, caused a significant increase in the yields of the spring wheat species under study, on average by 7.0%. Canopy spraying with algae extract had a favourable effect on the number of ears, on he number and weight of kernels per ear, but it had no effect on the weight of 1000 kernels. The grain quality of durum wheat, spelt wheat and common wheat was affected more strongly by the weather conditions in the successive years of the study and by the genotype than by the foliar application of algae extract. The spelt genotypes were characterised by lower yields and lower grain quality than common wheat and the durum wheat genotypes.

Genetic Analysis of Common Wheat Cultivar Taikong 6 for Resistance toHeterodera avenaeZhengzhou Population

2013 ◽  
Vol 39 (4) ◽  
pp. 642
Author(s):  
Jun-Li DAI ◽  
Lei CUI ◽  
Ke LIU ◽  
Ying-Ying ZONG ◽  
Hong-Xia YUAN ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Common Wheat ◽  
Wheat Cultivar ◽  
Common Wheat Cultivar

scholarly journals Comparative Analysis of Electrophoretic Profile of Major Proteins of Milk from Alpine and Carpathian Goats

2017 ◽  
Vol 74 (1) ◽  
pp. 20 ◽  
Author(s):  
Alina NASALEAN ◽  
Laurentiu OGNEAN ◽  
Sergiu MUNTEAN ◽  
Stefana BALICI ◽  
Horea MATEI
Keyword(s):  
Protein Profile ◽  
Milk Proteins ◽  
Goat Milk ◽  
Protein Composition ◽  
Raw Milk ◽  
Biologically Active ◽  
Protein Fractions ◽  
Animal Nutrition ◽  
Sds Page ◽  
Percentage Data

The milk’s proteins provide nutritional and biologically active values, essential in human and animal nutrition. In the case of goat milk, the proteins’ concentration and quality represent basic indices for the evaluation of the nutritional and biologically active values. The proposal is to comparatively analyse the protein profile of milk. The milk was collected from two different breeds: French Alpine and Romanian Carpathian. During March and April 2016 there were collected samples of raw milk in hygienic and sanitation conditions. There were two lots: first lot has 10 Carpathian goats and the second lot has 10 Alpine goats. The protein composition of goat milk was established with SDS-PAGE, after the evaluation of the total proteins’ concentration with the Bradford method. The quantitative and percentage data obtained with electrophoresis revealed few differences between those 8 identified protein fractions. Between those two lots, regarding the levels of β-CN, k-CN and β-lactoglobulines there were significant differences. The other protein fractions have values almost identical. Statistical analysis of obtained data shaped the differences in the protein profile at those two breeds. Based on those differences it is to note the superior potential of the Alpine breed regarding the content in biologically active milk proteins. Regarding the obtained data, this study brings new contributions for the evaluation and analysis of protein profile as a nutritive and biologically active component of goat milk, confirming its character as a functional aliment.

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