scholarly journals Study of wheat (Triticum aestivum L.) quality for feeding ruminants using in vitro and in vivo methods

2008 ◽  
Vol 53 (No. 6) ◽  
pp. 253-264 ◽  
Author(s):  
J. Pozdíšek ◽  
K. Vaculová
Keyword(s):  
Triticum Aestivum ◽  
Crude Protein ◽  
Nutritive Value ◽  
Nutrient Digestibility ◽  
Net Energy ◽  
Protein Nitrogen ◽  
Nitrogen Free Extract ◽  
Metabolisable Energy

: Nutrient digestibility and parameters of nutritive value for ruminants of two winter wheat (<I>Triticum aestivum</I> L.) cultivars were evaluated by means of an <I>in vivo</I> balance trial performed by the regression method on two groups of heifers with an increasing proportion of grain in DM (from 6 to 46%). Sulamit and Rapsodia, chosen on the basis of the <I>in vitro</I> test from a set of 25 cultivars (grown in 2002–2004) reached significant differences in DM fermentability <I>in vitro</I> (by 43.7–78.6 ml/g DM, <I>P</I> < 0.05). <I>In vivo</I> digestibility of crude protein, nitrogen-free extract, organic matter, parameters of N retention, energy concentrations (metabolisable energy, net energy for lactation and for fattening) and parameters in the PDI system (especially PDIE) increased along with the grain proportion in the diet. At the comparable proportion of grain in the ration the positive differences were significantly higher (<I>P</I> < 0.05) for Sulamit than Rapsodia.

scholarly journals Farklı Maltlık Arpa Çeşitlerinin Besin Değerlerinin In Vitro Gaz Üretim Tekniği Kullanılarak Tespiti

2017 ◽  
Vol 5 (10) ◽  
pp. 1216 ◽  
Author(s):  
Behlül Sevim ◽  
Tugay Ayaşan ◽  
İsmail Ülger ◽  
Şerife Ergül ◽  
Sait Aykanat ◽  
...  
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Net Energy ◽  
A Value ◽  
In Vitro Gas Production ◽  
Metabolisable Energy ◽  
Malt Barley

This study was conducted to determine the nutritive value of malt barley varieties using in vitro gas production technique and in vitro methane production. Atılır, Durusu and Fırat were used as three malt barley varieties. As a result of this study, between the varieties in terms of dry matter (DM), crude ash (CA), crude protein (CP), crude fat (CF), neutral detergent fiber (NDF) and hemicellulose (HEM) were found significantly important but differences between the varieties in terms of ADF were not found significant. The gas production rate of malt barley varieties ranged from 64.00 to 72.50 ml/200 mg DM. The metabolisable energy (ME) and net energy lactation (NEL) contents of malt barley varieties ranged from 11.75 to 12.86 MJ/kg DM and 7.16 to 7.98 MJ/kg DM respectively. The highest methane (CH4) content was obtained from the variety of Fırat with a value of 13.34 ml/200 mg DM, whereas the lowest value was obtained from the Atılır variety with a value of 12.16 ml/200 mg DM.

scholarly journals An in vitro method for studying digestion in the pig

1989 ◽  
Vol 61 (3) ◽  
pp. 689-698 ◽  
Author(s):  
H. Graham ◽  
W. Löwgren ◽  
P. Åman
Keyword(s):  
Small Intestine ◽  
Dietary Fibre ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutritive Value ◽  
Protein Nitrogen ◽  
In Vitro Method ◽  
Feed Samples

1. An in vitro method involving incubation of feed samples with buffered duodenal digesta for 12 h or with buffered ileal and faecal inocula for 48 h was compared with in vivo ileal or faecal apparent digestibilities in pigs.2. The five diets investigated had crude protein (nitrogen × 6.25) contents from 164 to 185 g/kg, starch contents from 296 to 463 g/kg and dietary fibre contents from 176 to 347 g/kg.3. In vitro disappearances with duodenal inocula were correlated (P < 0.05) with in vivo ileal apparent digestibilities for crude protein, ash, starch, energy and dry matter, but not for dietary fibre.4. In vitro disappearances with the ileal and faecal inocula were generally correlated (P < 0.1) with in vivo faecal apparent digestibilities for dietary fibre, energy and dry matter, but not for ash or crude protein. The patterns of degradability of fibre polysaccharide residues in vitro and in vivo were also similar.5. Results indicate that this in vitro method could be used to predict the availability of starch and crude protein for digestion in the small intestine, and the degradability of dietary fibre, and thus for comparing the nutritive value of pig feeds.

scholarly journals Determination of the In Vitro Gas Production and Potential Feed Value of Olive, Mulberry and Sour Orange Tree Leaves

2018 ◽  
Vol 13 (1) ◽  
pp. 269-278 ◽  
Author(s):  
Mustafa Olfaz ◽  
Unal Kilic ◽  
Mustafa Boga ◽  
Abdiwali Mohamoud Abdi
Keyword(s):  
Nutritive Value ◽  
Condensed Tannin ◽  
Gas Production ◽  
Net Energy ◽  
Sour Orange ◽  
Tree Leaves ◽  
Randomized Design ◽  
In Vitro Gas Production

AbstractThis study was conducted to determine the potential nutritive value andin vitrogas production (IVGP) parameters ofOlea europaea L. (Olive = OL),Morus alba L. (Mulberry = ML) andCitrus aurantium L. (Sour orange = SOL) tree leaves. Hohenheim gas test was used to determine thein vitrogas productions of the leaves. The gas production of samples over time was recorded for 3, 6, 9, 12, 24, 48, 72 and 96 h after incubation. Completely Randomized Design was used to compare gas production, and gas production kinetics of samples. The findings of the present study suggested that there were differences among the tree leaves in terms of crude protein, NDF,in vitrogas productions, organic matter digestibility (OMD), metabolisable energy (ME), net energy lactation (NEL) and relative feed values (RFV) (P<0.01). ML had the highest condensed tannin contents (P<0.05),in vitrogas production (IVGP), OMD and energy values (P<0.01). SOL had highest RFV values. OL showed the lowest IVGP when compared to SOL and ML. Low NDF and ADF contents of SOL would probably increase the voluntary intake, digestibility and relative feed values of these leaves by ruminants. In conclusion, it was determined that OL, ML and SOL used in the study have lowin vitrogas production and can be utilized as alternative roughage feed in ruminants. However, it is recommended that the results obtained from this research should be tested inin vivostudies.

scholarly journals Estimation of in vivo digestibility with the laying hen by an in vitro method using the intestinal fluid of the pig

1980 ◽  
Vol 43 (2) ◽  
pp. 389-391 ◽  
Author(s):  
K. Sakamoto ◽  
T. Asano ◽  
S. Furuya ◽  
S. Takahashi
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Laying Hen ◽  
Intestinal Fluid ◽  
Protein Nitrogen ◽  
In Vitro Method ◽  
Poultry Diets

1. Dry matter and crude protein (nitrogen × 6.25) digestibility of four poultry diets determined by an in vitro method using the intestinal fluid of pigs was significantly correlated with corresponding in vivo digestibility values obtained with hens.2. The intestinal fluid could be lyophilized and stored for at least 35 d without losing its activity on digestion.

Degradation Characteristics of Wheat, Barley and Oats Straws Untreated and Treated with Ammonia in an Oven

1988 ◽  
Vol 1988 ◽  
pp. 85-85
Author(s):  
Jeannie M Everington ◽  
D I Givens
Keyword(s):  
Elevated Temperature ◽  
Chemical Composition ◽  
Dry Matter ◽  
Nutritive Value ◽  
Good Prediction ◽  
Ammonia Treatment ◽  
Oven Method ◽  
Metabolisable Energy

Compared with stack treatment the literature contains little information on the nutritive value of cereal straws treated with ammonia in “ovens” at elevated temperature. Recently,Mason et al (1987) and Givens et al (1987) have studied in vitro and in vivo respectively the effect on chemical composition, digestibility and energy value of treating barley, wheat and oat straws with ammonia by the oven method. Substantial changes in chemical composition and nutritive value were found as a result of treatment.Reid and Ørskov (1987) have suggested that the dry matter (DM) degradation characteristics of straw as measured by the nylon bag technique provide a good prediction of its nutritive value. The aims of the experiment reported here were therefore to examine using the straws of Mason et al (1987), the effect of oven ammonia treatment on the DM degradation characteristics and to assess whether these parameters could be used to predict the metabolisable energy (ME) content of the straws.

scholarly journals In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4507
Author(s):  
Luiz Juliano Valério Geron ◽  
Fabiana Gomes da Costa ◽  
Silvia Cristina de Aguiar ◽  
Jocilaine Garcia ◽  
Matheus Gonçalves Ribeiro ◽  
...  
Keyword(s):  
Crude Protein ◽  
Ammonia Nitrogen ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Ruminal Fluid ◽  
Ph Values

<p>This study evaluated the digestibility of nutrients by, and parameters associated with, <em>in vitro </em>fermentation using different inocula (sheep ruminal fluid and feces) as well as the <em>in vivo </em>digestibility in sheep that were fed rations with 50% concentrate containing either no (0%) residue from the extraction of tamarind pulp (RETP) or 15% RETP. To determine the <em>in vitro </em>digestibility (<em>IV</em>D) of nutrients, two sheep, weighing 40.38 ± 2.10 kg, were used as inoculum donors. To determine the <em>in vivo </em>digestibility of nutrients, we used four sheep and a 3×2 factorial experimental design, with three methods of digestion of nutrients and two experimental rations (0% and 15% RETP). The variables were subjected to analysis of variance and the variables that showed differences at 5% probability were further analyzed using the Tukey test at 5% significance. The <em>IV</em>D using different inocula did not significantly differ (p&gt;0.05) from the <em>in vivo </em>digestibility in sheep for dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF). The different methods for determining nutrient digestibility did not affect (p&gt;0.05) the digestibility of DM, OM, crude protein (CP), NDF, and acid detergent fiber (ADF) in rations with 0% and 15% RETP. However, the <em>IV</em>D of CP for rations containing 0% and 15% RETP incubated with both inocula was lower (p&lt;0.05) than the CP digestibility <em>in vivo</em>. The <em>in vivo </em>digestibility of ADF for rations containing 0% and 15% RETP was higher (P&lt;0.05) than the <em>IV</em>D using sheep ruminal fluid and feces as inocula. The pH values and concentration of ammonia nitrogen (NH3-N) after <em>in vitro </em>incubation for 24 h and the <em>in vivo </em>assay were not different (p&gt;0.05) for the rations containing 0% and 15% RETP, but the pH and NH3-N of both fermented and rumen contents differed (p&lt;0.05) depending on the inocula used and the <em>in vivo </em>assay. In summary, the digestibility of DM, OM, and NDF can be determined by the <em>in vitro </em>fermentation method using the ruminal fluid or feces of sheep as inocula in rations containing 0% or 15% RETP. However, <em>in vitro </em>fermentation is not a suitable method for the determination of pH and NH3-N concentration.</p>

Nutritive value of Sesbania aculeata grown on salty soil and its effect on reproductive parameters of Syrian Awassi ewes

2005 ◽  
Vol 56 (8) ◽  
pp. 819 ◽  
Author(s):  
M. Zarkawi ◽  
M. R. Al-Masri ◽  
K. Khalifa
Keyword(s):  
Wheat Straw ◽  
Crude Protein ◽  
Nutritive Value ◽  
Soluble Nitrogen ◽  
Reproductive Parameters ◽  
Protein Nitrogen ◽  
Sesbania Aculeata ◽  
Whole Plant ◽  
Metabolisable Energy ◽  
Salty Soil

Nutritive value of Sesbania aculeata hay was determined by estimating the nutritive components, nitrogen forms, in vitro digestible organic matter (IVDOM), and metabolisable energy (ME). A study was carried out to evaluate the effect of feeding 2 groups of Syrian Awassi ewes with 2 wheat straw-concentrate diets without (control) or with (experimental) added S. aculeata hay on some reproductive parameters of the ewes at different stages. Half of the wheat straw in the daily ration of the experimental group of ewes was replaced by S. aculeata hay (whole plant) grown on salty soil and irrigated by saline water. Daily metabolisable energy and crude protein consumed by the ewes in the 2 groups were the same during the different stages (2.5 months before mating, throughout pregnancy, and during lactation). Crude fibre and cell wall constituents’ values were lower, whereas the values of crude protein and crude ash were higher in leaves and pods than in stalks. The values of IVDOM in S. aculeata hay were 537, 380, and 626 g/kg DM, and of ME were 7.19, 5.43, and 8.58 MJ/kg DM for the whole plant, stalks, and leaves and pods, respectively. The values of buffer soluble nitrogen were 27% or 39% and of buffer soluble non-protein nitrogen were 21% or 35% of the total nitrogen in leaves and pods or stalks, respectively. Feeding with S. aculeata hay had no effects on the liveweight of the ewes during the periods before mating and throughout pregnancy, on mating rate, fertility rate, duration of pregnancy, birth weight, or weaning weight of lambs. Furthermore, feeding with sesbania hay had no effect on either the response of ewes to the oestrous synchronisation programme or on the blood serum concentration and pattern of progesterone during the period extending from the commencement of feeding the ewes sesbania hay (2.5 months before mating) until the time of their lambing.

A note on in vivo comparison of a urea-formaldehyde-molasses complex and urea molasses mixes as sole nitrogen supplements to a wheat chaff ration for steers

1983 ◽  
Vol 36 (2) ◽  
pp. 309-311
Author(s):  
D. Lall ◽  
H. P. S. Makkar ◽  
S. S. Negi
Keyword(s):  
Crude Protein ◽  
Urea Formaldehyde ◽  
Nitrogen Retention ◽  
Protein Digestibility ◽  
Urea Derivative ◽  
Protein Nitrogen ◽  
Digestible Crude Protein ◽  
Wheat Chaff

ABSTRACTA urfia-formaldehyde-molasses complex appeared to be a promising slow ammonia-releasing urea derivative in in vitro studies. It was compared with urea-molasses mixtures as a non-protein nitrogen supplement to an ad libitum wheat chaff ration for steers. The significantly lower (P < 0·01) crude protein digestibility and intake of digestible crude protein, and the non-significantly lower nitrogen retention on the urea-formaldehyde-molasses complex ration revealed the superiority of simple urea-molasses mixtures as a non-protein nitrogen supplement.

scholarly journals In vivo and in vitro techniques for the assessment of the energy content of feed grains for poultry: a review

1999 ◽  
Vol 50 (5) ◽  
pp. 881 ◽  
Author(s):  
D. J. Farrell
Keyword(s):  
Near Infrared ◽  
Energy Content ◽  
Energy Systems ◽  
Great Promise ◽  
Net Energy ◽  
Near Infrared Reflectance ◽  
Food Grains ◽  
Metabolisable Energy

The focus of this paper is on the energy evaluation of foodstuffs, particularly of food grains for poultry. Apparent metabolisable energy (AME) is currently the preferred feeding system for poultry but net energy systems are future possibilities and one is currently being used in some poultry growth models. These systems take into account the efficiency with which AME is being utilised by the bird. Aspects such as AME adjusted to zero nitrogen retention and true metabolisable energy (TME) are discussed. Shortcomings of the AME system are also discussed and recent net energy systems are described briefly. The in vitro prediction of AME or TME has not shown great promise although the European Tables of Energy Values for Foodstuffs contain detailed analysis of chemical composition and AMEn data allowing improved accuracy of prediction. It is suggested that data generated on Australian grains should be tested using these tables, and if successful, these tables may be useful to industry. Near infrared reflectance analysis (NIRA) is likely to be the preferred in vitro method for predicting a range of characteristics of food grains including AME. A new method of grain evaluation developed in Canada is described and this approach, with modification, may be worth pursuing in Australia. For the in vivo method of measuring AME of grains, the classical total collection method with broiler chickens is recommended with minor changes. The use of acid-insoluble ash for estimating dry matter digestibility for subsequent determination of AME may have merit. A rapid assay appropriate to laying hens is proposed because of the known increase in AME of grains as birds age. It has been established that AME values generated using adult cockerels also apply reasonably well to hens.

scholarly journals  Nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methods

2012 ◽  
Vol 57 (No. 10) ◽  
pp. 454-468 ◽  
Author(s):  
P. Homolka ◽  
V. Koukolová ◽  
M. Podsedníček ◽  
A. Hlaváčková
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Energy Levels ◽  
Red Clover ◽  
Metabolizable Energy ◽  
Net Energy ◽  
Gross Energy ◽  
Model Expression

The aim of this study was to determine the nutrient and energy levels of red clover and lucerne forage. Investigation of forage at different maturity stages of three growths was carried out by chemical analysis, in vitro and in vivo digestibility methods.&nbsp; Generally, maturation caused a significant increase in fibre fractions. With the increasing maturity of forage samples the in vivo, in vitro, and calculated in vivo (in vivo<sub>calcul</sub>) digestibilities of organic matter (OM) linearly decreased. The in vitro and in vivo<sub>calcul</sub> digestibilities of OM averaged 0.754 and 0.708 for red clover and 0.717 and 0.667 for lucerne, respectively. The in vivo OM digestibility averaged 0.710 for red clover and 0.666 for lucerne. Gross energy (GE), digestible energy (DE), metabolizable energy (ME), net energy for lactation (NEL), and net energy for growth (NEG) averaged 18.12, 12.41, 9.60, 5.67, 5.50 and 18.09, 11.56, 9.01, 5.26, 4.99 MJ/kg of dry matter for red clover and lucerne, respectively. The effect of a vegetative stage on energy values of both forages was diverged for various growth times. When data were pooled across the estimated season, seven cutting-specific equations for descriptions of GE, DE, ME, NEL, NEG, in vitro, and in vivo OM digestibilities were obtained for red clover and lucerne, separately. The red clover model expression gave similar prediction equations for lucerne. It was possible to predict cutting-specific equations with coefficients of determination R<sup>2</sup> &gt; 0.719 for red clover and R<sup>2</sup> &gt; 0.400 for lucerne of the variation in GE, DE, ME, NEL, and NEG. The in vitro and in vivo OM digestibility equations were predicted with R<sup>2 </sup>being 0.840 (in vitro) and 0.707 (in vivo) for red clover, and 0.979 (in vitro) and 0.937 (in vivo) for lucerne. The parameters of these specific equations were statistically preferable than the general model expression which included both forages together. &nbsp;

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