scholarly journals Cryptocline taxicola (All.) Petr. – A new plant pathogen reported in Slovak Republic – Short Communication

2008 ◽  
Vol 43 (No. 3) ◽  
pp. 122-124 ◽  
Author(s):  
N. Bukvayová
Keyword(s):  
Fungal Infection ◽  
Plant Pathogen ◽  
Short Communication ◽  
Slovak Republic ◽  
Urban Park ◽  
Taxus Baccata ◽  
First Report ◽  
Visual Symptoms ◽  
Microscopic Features

Symptoms of fungal infection were observed on a plant of <i>Taxus baccata</i> in an urban park. Diagnosis of the pathogen showed the presence of the fungus <i>Cryptocline taxicola</i>. This is the first report of this fungus in Slovak Republic. Characteristics of visual symptoms and microscopic features are described.

scholarly journals Occurrence of rust disease caused by Puccinia oxalidis on Oxalis triangularis in the Czech Republic – Short Communication 

2014 ◽  
Vol 50 (No. 1) ◽  
pp. 17-18
Author(s):  
I. Šafránková
Keyword(s):  
Czech Republic ◽  
Leaf Spot ◽  
Short Communication ◽  
Rust Disease ◽  
The Czech Republic ◽  
First Report ◽  
Microscopic Features

This is the first report of&nbsp;Puccinia oxalidis&nbsp;causing leaf spot diseases on ornamental&nbsp;Oxalis triangularis&nbsp;subsp.&nbsp;papilionaceae&nbsp;cv. Atropurpurea in Moravia, Czech Republic. The macroscopic symptoms and microscopic features are described.

Molecular characterization and first report ofCryptosporidiumgenotypes in human population in the Slovak Republic

2015 ◽  
Vol 36 (23) ◽  
pp. 2925-2930 ◽  
Author(s):  
Antónia Petrincová ◽  
Alexandra Valenčáková ◽  
Lenka Luptáková ◽  
František Ondriska ◽  
Jana Kalinová ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Human Population ◽  
Slovak Republic ◽  
First Report

scholarly journals  Leaf spot and dieback of Buxus caused by Cylindrocladium buxicola

2013 ◽  
Vol 49 (No. 4) ◽  
pp. 165-168 ◽  
Author(s):  
I. Šafránková ◽  
L. Holková ◽  
M. Kmoch
Keyword(s):  
Dna Sequencing ◽  
Leaf Spot ◽  
Mycelial Growth ◽  
Leaf Blight ◽  
Visual Symptoms ◽  
Buxus Sempervirens ◽  
Microscopic Features ◽  
South Moravia ◽  
Molecular Characters

Leaf blight symptoms were observed on potted box plants (Buxus sempervirens cv. Suffruticosa and B. microphylla) in a nursery in South Moravia in August 2010. These symptoms were suggestive of box blight on Buxus spp. Characteristics of the visual symptoms, microscopic features, and identification of the isolates using DNA sequencing are described. The causal agent was isolated and identified as Cylindrocladium buxicola Henricot by means of morphological, cultural, and molecular characters. The effect of five commercial fungicides on C. buxicola in vitro was studied. The most effective fungicides (kresoxim-methyl, azoxystrobin, and mancozeb) inhibited conidia germination and mycelial growth (kresoxim-methyl, myclobutanil, and penconazole) of C. buxicola more than 96%. &nbsp;

scholarly journals Short Communication: Taxonomy and the first report for pollen and leaf surfaces anatomy of the genus Enkleia (Thymelaeaceae) in Thailand

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Piyaporn Saensouik ◽  
Surapon Saensouk
Keyword(s):  
Short Communication ◽  
Conservation Status ◽  
Distribution Data ◽  
Flowering Period ◽  
Ecological Data ◽  
Traditional Use ◽  
First Report ◽  
Leaf Surfaces

Abstract. Saensouk P, Saensouk S. 2021. Short Communication: Taxonomy and the first report for pollen and leaf surfaces anatomy of the genus Enkleia (Thymelaeaceae) in Thailand. Biodiversitas 22: 1340-1344. Taxonomic of the genus Enkleia in Thailand are revised. Two species, Enkleia malaccensis Griff. and E. thorelii (Lecomte) Nevling, are reported. Enkleia siamensis (Kurz) Nevling and E. siamensis (Kurz) Nevling subsp. andamanica (Hutch. Ex C. E. Parkinson) Nevling are reduced to Enkleia malaccensis Griff.. Therefore, both species are recorded as synonym of E. malaccensis. E. thorelii is reported endemic to Thailand. Vernacular name, distribution data, ecological data, flowering period, fruiting period, conservation status, traditional use of this genus are provided. The pollen and leaf surfaces of the genus are examined and the first studied.

scholarly journals First Report of a Leaf Blight Caused by Pyricularia pennisetigena on Cenchrus echinatus in Paraguay

Plant Disease ◽  
2021 ◽  
Author(s):  
Cinthia C. Cazal-Martínez ◽  
Yessica Magaliz Reyes Caballero ◽  
Alice Chávez ◽  
Pastor Enmanuel Pérez Estigarribia ◽  
Alcides Rojas ◽  
...  
Keyword(s):  
Rna Polymerase Ii ◽  
Large Subunit ◽  
Fungal Species ◽  
Leaf Blight ◽  
Internal Transcribed Spacers ◽  
Conidial Suspension ◽  
Sterile Water ◽  
Potential Threat ◽  
First Report ◽  
Microscopic Features

The genus Pyricularia contains several fungal species known to cause diseases on plants in the Poaceae family (Klaubauf et al. 2014; Wang et al. 2019). While sampling for P. oryzae during March-2015 and April-2018, common weed Cenchrus echinatus L. was observed with leaf lesions in and around experimental wheat fields in the departments of Canindeyú and Itapúa. C. echinatus samples from both locations displayed similar leaf lesions, varying from small light brown pinpoint to elliptical brown lesions with greyish center. Symptomatic leaves were surface disinfested and cultured on potato dextrose agar (PDA) amended with 1% gentamicin at 25°C. Two monosporic isolates were obtained, one from Itapúa (ITCeh117) and the other from Canindeyú (YCeh55). The isolates were subsequently grown on oatmeal agar (OA) and PDA under a 12-h photoperiod at 25°C and evaluated after ten days for colony diameter, sporulation, macroscopic and microscopic features. Colonies on OA reached up to 4.8 cm diameter and were light grey, whereas colonies on PDA reached up to 5.3 cm diameter and were brown with grey centers, with cottony mycelium and broad white rims. Mycelium consisted of smooth, hyaline, branched, septate hyphae 4-4.5 µm diameter. Conidiophores were erect, straight or curved, unbranched, medium brown and smooth. Conidia were solitary, pyriform, pale brown, smooth, granular, 2-septate, 32-33 × 9-10 μm; truncated with protruding hilum and varied in length from 1.0 to 1.5 μm and diameters from 2.0 to 2.2 μm. Both isolates were similar and identified as Pyricularia pennisetigena, according to morphological and morphometric characteristics (Klaubauf et al. 2014). Subsequently, genomic DNA was extracted from each isolate using the primers described in Klaubauf et al. (2014) to amplify and sequence the internal transcribed spacers (ITS), partial large subunit (LSU), partial RNA polymerase II large subunit gene (RPB1), partial actin gene (ACT), and partial calmodulin gene (CAL). Sequences from each isolate (YCeh55/ITCeh117) were deposited in GenBank with the following submission ID for ITS: MN947521/MN947526, RPB1: MN984710/MN984715, LSU: MN944829/MN944834, ACT: MN917177/MN917182, and CAL: MN984688/MN984693. Phylogenetic analysis was conducted using the software Beast v1.10.4. The results obtained from the concatenated matrix of the five loci placed these isolates in the P. pennisetigena clade. To confirm pathogenicity, each isolate was adjusted to 5×104 conidia/ml of sterile water and C. echinatus plants were sprayed with the conidial suspension for isolate YCeh55, ITCeh117 or sterile water using an oilless airbrush sprayer until runoff. The three treatments were kept in the greenhouse at 25-28°C and about 75% relative humidity under natural daylight. Each treatment included three to five inoculated plants and 10 leaves were evaluated per treatment. Symptoms were observed 8-15 days after inoculation and were similar to those originally observed in the field for both isolates, whereas the control plants remained asymptomatic. P. pennisetigena was re-isolated from the inoculated leaves fulfilling Koch’s postulates. To our knowledge, this is the first report of leaf blight on C. echinatus caused by P. pennisetigena in Paraguay. The occurrence of P. pennisetigena in the region and its ability to infect economically important crops such as wheat and barley (Klaubauf et al. 2014; Reges et al., 2016, 2018) pose a potential threat to agriculture in Paraguay.

scholarly journals First Report of Cryptocline taxicola Infecting Pacific Yew (Taxus brevifolia) in Eastern North America

Plant Disease ◽  
2001 ◽  
Vol 85 (8) ◽  
pp. 922-922 ◽  
Author(s):  
Vladimir Vujanovic ◽  
Marc St-Arnaud
Keyword(s):  
North America ◽  
Soil Layer ◽  
Organic Soil ◽  
Botanical Garden ◽  
The United States ◽  
Taxus Baccata ◽  
Conidial Suspension ◽  
Taxus Brevifolia ◽  
Rock Outcrops ◽  
First Report

To our knowledge, this is the first report of Cryptocline taxicola (Allesch.) Petrak (Coelomycetes) on Pacific yew (Taxus brevifolia Nuttall) and the first observation of the fungus infecting living needles. C. taxicola is known to occur on needles of Taxus baccata in Europe (on T. baccata var. fastigiata) and North America (Vermont) (on T. baccata var. canadensis) (2). In August 1999 and 2000, chlorotic and necrotic current and second-year needles of Pacific yew were observed to have fruiting bodies, sometimes greater than 400 per needle, on the upper surface. Acervuli were circular to subcircular, yellowish, surrounded by brown circles, subcuticular to intraepidermal, at first covered, later exposed by the fissure of the cuticule, and 150 to 350 μm wide. Hyphae in host tissue were septate, brownish, and 3 to 4.5 μm in diameter. Conidiophores were phialidic, cylindrical, hyaline, 10 to 20 × 2.5 to 4.5 μm. Mature conidia were ellipsoidal to oval, truncate at the base, obtuse at the apex, hyaline to slightly pigmented, 8 to 17 × 4 to 5 μm. From these symptomatic needles, C. taxicola was often isolated (>85%) on potato dextrose agar. Koch's postulates were completed for C. taxicola by spraying current-year living needles, on one twig of each of separate plants (five) of T. brevifolia with a conidial suspension of 4 × 103 conidia per ml. Five control twigs were sprayed with sterile, distilled water. Twigs were covered with black sterile plastic bags and incubated outdoors for 24 h, when the bags were removed. Within 3 weeks, inoculated needles exhibited chlorotic and necrotic symptoms similar to those originally observed, but symptoms were never observed on control twigs. The fungus was reisolated (91%) only from the symptomatic needles. T. brevifolia trees growing in the Montreal Botanical Garden (Quebec, Canada) are clones originating from the Pacific Coast of the United States. We found that Pacific yew was colonized more frequently on the dry rock outcrops in the Alpine and Chinese Garden tree plantations. We believe that inappropriate stand selection, unfavorable humid conditions, and a thin organic soil layer may predispose Pacific yew to infection by this fungal species. A similar effect has been reported in Europe (1) for Cryptocline pseudotsugae causing serious levels of disease in a Pseudotsuga menziesii plantations. References: (1) T. L. Cech. Forstschutz Aktuell. 25:13, 2000. (2) G. Morgan-Jones. Can. J. Bot. 51:309, 1973.

scholarly journals First report of Pepper mild mottle virus in pepper seeds produced in the Czech Republic – Short Communication

2010 ◽  
Vol 42 (No. 1) ◽  
pp. 34-37 ◽  
Author(s):  
J. Svoboda ◽  
G. Červená ◽  
J. Rodová ◽  
M. Jokeš
Keyword(s):  
Czech Republic ◽  
Electron Microscope ◽  
Resistance Genes ◽  
Short Communication ◽  
Mottle Virus ◽  
Pepper Mild Mottle Virus ◽  
The Czech Republic ◽  
First Report ◽  
Viral Particles ◽  
Straight Rod

Symptoms of viral infection were observed on plants of pepper, cv. OL 228, raised from commercial seeds of Czech origin in a greenhouse in the year 2002. Infected plants showed mosaic or mottling on leaves, and necrotic depressions on fruits. Straight, rod-shaped viral particles of about 300 nm, indicating a tobamovirus infection, were found by electron microscope. ELISA produced negative reactions for <i>Tobacco mosaic virus</i> (TMV) but positive reactions with an antiserum to <i>Pepper mild mottle virus</i> (PMMoV). In biological characterisation using pepper cultivars with the L1, L2, L3 and L4 tobamovirus resistance genes it was found that the Czech isolate of PMMoV belongs to pathotype P1.2. This is the first report of PMMoV in the Czech Republic. Its distribution, however, may still be limited as a survey did not reveal other infections in the main pepper producing areas. As PMMoV spreads with infected seeds, the possibility of its chemical deactivation by NaOH was tested and confirmed.

Short communication: first report of nematodes parasitizing the four-eyed-fish, Anableps anableps (Pisces, Cyprinodontiformes)

2017 ◽  
Vol 116 (8) ◽  
pp. 2249-2254 ◽  
Author(s):  
Juliana Souza Ribeiro ◽  
Francisco Carlos Rodrigues de Oliveira ◽  
Nicole Brand Ederli
Keyword(s):  
Short Communication ◽  
First Report

scholarly journals First Report of Westerdykella dispersa as a Cause of an Angioinvasive Fungal Infection in a Neutropenic Host

2014 ◽  
Vol 52 (12) ◽  
pp. 4407-4411 ◽  
Author(s):  
P. K. Sue ◽  
G. T. Gurda ◽  
R. Lee ◽  
T. Watkins ◽  
R. Green ◽  
...  
Keyword(s):  
Fungal Infection ◽  
First Report

scholarly journals Auffällige Stammkrebse an Eiben (Taxus baccata L.) im Fürstenwald bei Chur (Graubünden, Schweiz) | Stem Canker of Yew Trees (Taxus baccata L.) in the Fürstenwald near Chur, Canton Grisons, Switzerland

2004 ◽  
Vol 155 (9) ◽  
pp. 400-403 ◽  
Author(s):  
Jürg Hassler ◽  
Werner Schoch ◽  
Roland Engesser
Keyword(s):  
Fungal Infection ◽  
Fungal Infections ◽  
Stem Canker ◽  
Taxus Baccata

The yew (Taxus baccata L.) is not generally considered to be susceptible to fungal infections or destructive insects. In the Fürstenwald near Chur (Canton Grisons,Switzerland) however, every fourth yew in a population manifests widespread stem canker. Fungal infection is suspected to be the cause of the cankerous growth.

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