scholarly journals Missense mutations in exon 2 of the porcine leptin receptor gene and their associations with litter size and body weight

2009 ◽  
Vol 54 (No. 5) ◽  
pp. 210-216 ◽  
Author(s):  
C. Sun ◽  
L. Wang ◽  
D.F. Jiang ◽  
B. Zhang

Leptin receptor (<I>LEPR</I>) gene is regarded as a “candidate-gene” of production traits. The aims of this study were to detect polymorphisms of exon 2 within <I>LEPR</I> gene and to investigate their associations with production traits, litter size and live weight in Luchuan and Large White pig breeds. For this purpose, the single nucleotide polymorphisms (SNPs) of exon 2 within <I>LEPR</I> were detected using the PCR-SSCP procedure and their association with litter size and live weight was also analysed in Luchuan (<I>n</I> = 446) and Large White pigs (<I>n</I> = 405). The results showed that the C155T mutation was found in exon 2 of porcine <I>LEPR</I> gene in analyzed populations, which caused a missense mutation (Met to Thr). The different genotypes of this locus had the effects on total number of piglets born, born alive of the first (1<sup>st</sup>) and the first to the fourth (1<sup>st</sup>–4<sup>th</sup>) litters. The individuals with allele <I>A</I> had a higher number of total piglets born and a higher number of born alive piglets in the first litter (<I>P</I> < 0.05) while the differences in birth weight, weaning weight and average daily gain were not significant among different genotypes of this locus in analyzed populations. It implies that the exon 2 (Thr/Met) mutation of <I>LEPR</I> gene is a potential gene marker of pig reproduction. Therefore, it can be used in the marker-assisted selection (MAS) of pig breeding work.

2011 ◽  
Vol 49 (No. 9) ◽  
pp. 373-382 ◽  
Author(s):  
E. Goliášová ◽  
J. Wolf

The effect of PvuII polymorphism of the oestrogen receptor gene on litter size and production traits in a Czech Large White population (882 sows, 2 455 litters) was evaluated. Data were analysed by four four-trait animal models. The traits analysed were lifetime average daily gain in the field test, lean meat percentage, number of piglets born alive in parity 1 and number of piglets born alive in parity 2 and subsequent parities. The animal models differed in considering the herd-year-season effect as random or fixed and in including or not including the dominance effect. For each model two variants were calculated: the ESR effects were calculated either across herds or within herds. On the genetic level, no significant overall effects of the ESR gene were detected. The effects of the ESR gene within herds were mostly insignificant as well. There was a certain tendency for allele A to increase litter size in parity 1. Nevertheless, on the basis of the present knowledge, the use of the ESR gene for selection for litter size in the Czech Large White breed seems to be of questionable benefit and cannot be recommended. &nbsp;


2008 ◽  
Vol 52 (No. 10) ◽  
pp. 334-340 ◽  
Author(s):  
P. Humpolíček ◽  
T. Urban ◽  
V. Matoušek ◽  
Z. Tvrdoň

The study was designed to determine the associations between the Estrogen Receptor Gene (<i>ESR - PvuII</i>), Follicle Stimulating Hormone Gene (<i>FSHB - HaeIII</i>) and Myogenin Gene (<i>MYOG - MspI</i>) with traits characterizing the sow efficiency comprehensively. Concretely, the age of the first conception (AFC), service period (SP), insemination interval (InI), total number of piglets born (TNB), number of piglets born alive (NBA), number of piglets weaned (NW), lean meat content (LMC), backfat thickness (BFT) and average daily gain (ADG) were included in the study. Significant effects of <i>ESR</i> and <i>FSHB</i> genes on litter size (particularly in the second parity) and on reproduction traits were found in the investigated population. The influence of <i>MYOG</i> gene on reproduction traits was observed but there was no effect of this gene on litter size. None of the included genes had an effect on growth traits. The results outline the possible application of the above-mentioned genes in selection of Large White sows.


Author(s):  
O. Khmeliova ◽  
R. Stavetska

The article highlights the problem of finding optimal variants of breeding process in a herd of pigs at purebred and crossbred selection. For this purpose, the control and two experimental groups were formed: ♀ Pietrain × ♂ Pietrain (control), ♀ (Pietrain × Large White) × ♂ Large White (first experimental group), ♀ (Pietrain × Durok) × ♂ Pitren (second experimental group). The analysis of the animals performance in these groups was carried out in 40, 30 and 35 parities, respectively. The research was conducted on the basis of PE «Maglevovany M.I.» in the Donetsk region. It has been established that the higher level of reproductive characteristics have sows of first experimental group, which have heredity of Large White breed, this breed is traditionally considered as a maternal form in cross-breeding schemes. For instance, their litter size at birth is higher than the same indicator in the control and second experimental group (plus 0,5, P<0,05, and 0,3 piglets, respectively); by the litter size at weaning at 28 days (plus 1,6, P<0,001, and 0,6 piglets); by the litter size at 2 months (plus 1,6, P<0,001, and 0,4 piglets). The highest piglet survival at the age of 2 months was in the litters, whose ancestor were boars of Pietrain breed (second experimental group) − plus 3,6 and 2,3 %, respectively, compered to purebred piglets of Pietrain breed and the first experimental group. For such complex indexes as the litter weight at weaning and litter weight at 2 months the first experimental group had an advantage of 18,1 kg (P<0,001) and 31,1 kg (P<0,001) over the control group (purebred Pietrain) and 5,4 kg and 7,6 kg − over the second experimental group. However, the average piglet weight of sows of second experimental group both at 28 days and at 2 months are equal to the average piglet weight of the same age of the first experimental group. The piglets, which are descendants of boars of meat breeds Duroc and Pietrain show a tendency to the faster growth even in an early age. The avarage live weight of pigs of the second experimental group at the age of 100 days and 7 months is higher compared to avarage live weight of pigs of the same age of the control and the first experimental groups – plus 8,5–21,7 kg (P<0,01 for the second experimental group) and 4,4–13,5 kg (P<0,001 for the second experimental group), respectively. At the age of 100 days, the maximum average daily gain was obtained in the second experimental group with an advantage of 190 g (P<0,001) compared to control group and 115 g (P<0,001) compared to the first experimental group. It is obvious that hybrids with the faster growth, which were obtained through crossbreeding ♀ (Pietrain × Duroc) × ♂ Pietrain reach a live weight of 100 kg in a shorter period: they need less time needed to reach 100 kg of live weight – 32 days less (P<0,001 ) compared to purebred animals of Pietrain breed (control group) and 18 days less (P<0,001) compared to hybrids ♀ (Pietrain × Large White) × ♂ Large White (the first control group). By such an indicator as the fat thickness the hybrids of the second experimental group dominate by purebred pigs of the same age of the control group (minus 0,2 mm), that characterize the low ability of meat breed for getting fat. The fat thickness of hybrids of the second experimental group was less by 0,9 mm (P<0,001) compared to the pigs of the first experimental group. Thus, in this herd in order to improve the reproductive qualities of sows, it is suggested to use the crossbreeding scheme ♀ (Pietrain × Large White) × ♂ Large White and to improve the fattening qualities of young animals the optimal is scheme of crossbreeding ♀ (Pietrain × Duroc) × ♂ Pietrain. Key words: pigs, Pietrain, Large White, Duroc, purebred and crossbred selection, reproductive and fattening qualities.


2016 ◽  
Vol 3 (2) ◽  
pp. 42-48
Author(s):  
V. Balatsky ◽  
I. Bankovska ◽  
A. Saienko

Leptin receptor is one of the components of the system of regulating energy homeostasis of the organism. Leptin receptor gene (LEPR) polymorphism is associated with pig carcass index of the content of intramus- cular fat in its valuable parts, which is particularly important when assessing the quality of their carcasses for processing. Intramuscular fat is associated with meat fl avor characteristics and partly determines its tenderness, juiciness, and other parameters. Aim. To analyze LEPR gene (SNP NM001024587.1, p. 1987 C > T) polymor- phism in populations of various pig breeds and to establish its relationship with the quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. Methods. Genetic-population analysis of nine pig breeds, associative analysis on the search connection of LEPR gene polymorphism with quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. LEPR locus genotyping was performed by High Resolution Melting (HRM). Results. All the studied breeds are characterized by polymorphism of the leptin receptor gene (SNP NM001024587.1, p. 1987 C > T), signifi cant breed specifi city in the distribution of frequencies of alleles was established. Statistically confi rmed effect (p < 0.05) of genotypes LEPR on the content of intramuscular fat, total dry matter and moisture in the meat, as well as the moisture content in the back fat of pigs of Ukrainian Large White breed was revealed. Higher content of intramuscular fat was found in the animals with genotype TT, while a smaller amount of intramuscular fat and more moisture in fat was revealed in heterozygotes. Conclusions. Genetic marker LEPR SNP NM001024587.1, p. 1987 C > T can be used in the marker-assisted selection to predict and improve the performance quality of the meat of pigs of Large White breed of the Ukrainian breeding. These results suggest that porcine leptin receptor gene controls the quality of fat comp- lex – inside muscles and in the dorsal part of the carcass.


Author(s):  
Viktor Khalak ◽  
Vasyl Voloshchuk ◽  
Konstiantyn Pochernyaev ◽  
Serhii Smyslov ◽  
Maria Ilchenko

The results of studies of the reproductive ability of sows of different genotypes are given taking into account the polymorphism g.1426G> A of the MC4R gene, the factor of repeatability of characters is determined, and the economic efficiency of the research results is calculated. The experimental part of the research was carried out in the conditions of the «Druzhba-Kaznacheevka» LTD in Dnipropetrovsk region. The objects of research were sows of the Large White breed. It was determined that sows of the main herd, according to signs of reproductive ability, belong to class I and class elite. The experiment showed that sows of genotype AG exceeded their peers of others (GG and AA) in terms of “born piglets total, heads” by 1.6 and 0.7 heads, “Live piglets born (multiple births), heads - by 1 6 and 0.9 heads, “the mass of the litter at the time of birth, kg” - by 2.7 - 1.3 kg., “the mass of the litter at the time of weaning at the age of 28-35 days, kg” - by 5, 1 - 3.9 kg. The difference between animals of the indicated genotypes is: for large-foetus - 0.06-0.01 kg, the index "evenness of the sow’s litter in live weight of piglets at birth, points" - 1.05-1.84 points, index of N. D. Berezovsky - 3.75 -2.36 points. The index of the safety of piglets before weaning ranged from 84.4 to 93.0 %. It was proved that the minimum value of the index “evenness of the sow’s littar in live weight of piglets at the time of birth, points (7.76 ± 1,082 points) are characterized by sows of genotype AA, in which the rate of multiple pregnancy ranged from 8 to 14 pigs, and the value of the sign“ large-foetus, kg "equaled 1.37 kg. The repeatability coefficient for sows of the main herd and sows of the Large White breed of different genotypes for the melanocortin 4 (MC4R) receptor gene ranges from -0.916 ± 0.1418 (І-IV farrow, genotype - GG, sign - “piglet safety before weaning, %) to 0.978 ± 0.0738 (I-V farrow, genotype - GG, sign - “large foetus”, kg ”). The maximum value of the phenotypic consolidation coefficient was found in sows of genotype AA according to the following quantitative characteristics: “live piglets (multiple births), heads.”, “litter weight at the time of birth, kg”, “litter weight at weaning age of 28-35 days, kg "," the safety of piglets before weaning ,% ". The sows of the genotype GG belong to the unconsolidated group according to the signs of reproductive qualities. The use of sows of the genotype for the melanocortin 4 (MC4R) AG receptor gene provides additional products at the level of 5.15 %, and its cost is 165.41 UAH /head. Keywords: sow, breed, reproductive ability, MC4R gene, genotypic consolidation coefficient, variability, correlation, repeatability.


1996 ◽  
Vol 1996 ◽  
pp. 18-18
Author(s):  
O.I. Southwood ◽  
S. Hoste ◽  
T.H. Short ◽  
A.J. Mileham ◽  
D. Cuthbert-Heavens

A significant relationship between the oestrogen receptor gene (ESR) and litter size has been detected in USA populations of Large White and a synthetic comprising 50% Meishan (Rothschild et al., 1995). Animals carrying two copies of the favourable allele (B) had an extra pig born per litter than those that did not have the allele. This paper reports on results observed in a UK 50% Meishan synthetic and four UK Large White lines.Litter size data from 50% Meishan synthetic (L93) full-sib females where more than one ESR genotype was segregating. Data were analysed using a mixed model with full relationships and including the fixed effects of season of farrowing, parity, ESR genotype (AA, AB or BB) and service type (AI or natural service). Heritiability and permanent environmental effects for litter size were assumed as 0.09 and 0.11, repectively. A total of 27 full-sib families were represented and included 62 sows and 139 litter records. Hypothesis testing used the option in PEST under a mixed model (Groeneveld et al., 1991).


Author(s):  
M.G. CHABAEV ◽  
◽  
E.YU. TSIS ◽  
R.V. NEKRASOV ◽  
M.I. KLEMENTEV

Selenium plays an important role for maintaining complex and adequate nutrition of fattened young pigs. It also has a big biological importance to maintain growth and health, as well as for biochemical and physiological processes This study presents the results of a production experiment to determine the effect of organic and inorganic forms of selenium on growth indicators, immunohematological blood parameters and slaughter qualities of fattened pigs in period I and II. Test feeding with various forms of selenium has been implemented with two groups of analogs (N = 60; n = 30), taking into account age and live weight. The experiment was conducted on castrates of large white breed, which were divided into two groups of 30 heads each following the principle of analogues (taking into account their age, live weight). The studied fattened pigs received standard complete feeds of the SK-5 and SK-6 types for a period of 105 days. Growing pigs were fed with organic selenium for 20 mg/kg as part of the feed 64 mixture per head in the first and second period of fattening, while the animals of the control group were fed with 0.3 mg/kg of the inorganic form of selenium. Including a chelated form of selenium in the complete feed of castrates at the rate established in the course of scientific and economic studies has led to 757 g of average daily gain, which is 8.1% more as compared to the control group. The concentration of red blood cells in the blood of growing pigs fed with 20 mg/kg of selenium chelate increased by 6.3%, hemoglobin – by 2.4%, and white blood cells – by 7.1% as compared to the control. Feeding organic selenium to fattened young pigs contributed to a significant increase in specific units of protein activity by 38.65% (p < 0.01), BASC – by 7.63%, as well as lysis – by 40.06% (p < 0.05), and phagocytic activity by 3.33% in blood serum as compared to the control. The half-carcasses of hogs who had received fed rations including selenium chelate fetured slaughter weight and slaughter yield, respectively, higher – by 6.4 and 0.6% as compared with the control analogues. The use of the established norm of organic selenium in the diets of fattened young pigs according to the results of production testing is economically profitable and contributes to a per-head profit of 420 rubles.


1973 ◽  
Vol 17 (3) ◽  
pp. 325-328
Author(s):  
J. P. Chadwick ◽  
W. C. Smith

SUMMARYTwo Poland China boars were mated with 12 Large White females and two Large White boars were used on the same number of females of similar background. In the post-weaning period a trial was conducted to compare the performance of purebred and crossbred progeny given a grower's diet on an ad libitum scale of feeding over the live-weight range 28 to 66 kg. Breed of boar did not influence litter size nor total litter weight at either birth or weaning. Poland China crosses compared with Large Whites grew more slowly to slaughter (10 %) but with equal efficiency of live-weight gain. Cross-breds had higher carcass yields (4 %), shorter (3 %) and deeper (2 %) carcasses, lower depths of mid-line and internal fat (11 %) and larger eye-muscle areas (22%). Breed of boar had little influence on joint proportions and no effect on carcass cut-out value. The eye muscles of crossbred carcasses were paler in colour and had higher transmission values but drip loss from the meat was not different.


2020 ◽  
Vol 50 (4) ◽  
pp. 537-551
Author(s):  
T.S. Brand ◽  
J. Van der Merwe ◽  
L.C. Hoffman

Canola meal (CM) is a locally produced protein source that may be less expensive than soybean meal (SBM). This study evaluated the effects of replacing 0%, 25%, 50%, 75%, and 100% SBM with CM in diets for slaughter ostriches. The CM was added at the expense of SBM and other concentrates, with minor changes in other ingredients. Birds (n = 15 per treatment) were reared from 77 to 337 days old on the trial diets, which were supplied ad libitum for starter, grower, and finisher phases. Bodyweights and feed intake were measured during these phases. No differences (P >0.05) were found between treatments for live weight at the end of each phase, dry matter intake (DMI), average daily gain (ADG) and feed conversion ratio (FCR) over all the growth phases. Although no differences were observed in live weight at the end of each phase, the birds reared on the diet with 50% CM were heaviest at slaughter, and birds reared with 100% CM were lightest (P <0.05). Differences (P <0.05) between diets were observed for the weight at slaughter, weights of the liver and thyroid glands and the pH of the cold carcass. However, no differences (P >0.05) were observed between diets for fat pad weight, dressing percentage, and weights of thighs and Muscularis gastrocnemius. The results indicate that CM could replace SBM in the diets of slaughter ostriches without affecting production traits and slaughter yields.Keywords: alternative protein, average daily gain, canola, dry matter intake, feed conversion ratio, growth, ostrich nutrition, production


2020 ◽  
Vol 60 ◽  
pp. 5-11
Author(s):  
I. V. Verbuch ◽  
H. V. Bratkovska

Goal. To accelerate the selection process and create populations of pigs with high level of productivity to assess the most effective modern methods breeding value of large white pigs in the SE "DG" Pasichna "Institute of Feed and Agriculture Podillya NAAS" Starosinyavsky district of Khmelnytsky region on the basis of a combined assessment of repair young for their own productivity and assessment meat qualities by MD Berezovsky and B. Tyler and the most promising method BLUP. Methods. Comparison, zootechnical and biometric analyzes. Results. The breeding value of pigs of large white breed of the breeding farm of SE "Pasichna" ICSGP NAAS "Starosinyavsky district of Khmelnytsky region by modern effective methods, namely: BLUP. According to the average indicators of the combined assessment of own productivity of animals at control fattening, taking into account their live weight on the date of birth and weaning, average daily gain, live weight and age of 100 kg, body length and fat thickness, boars significantly outweighed pigs. Compared with pigs, their live weight at the date of birth and weaning (at the age of 30 days) was higher by 0.1 and 0.81 kg. The age of reaching a live weight of 100 kg exceeded by 5.43 days, the average daily gain of 0.016 kg. Live weight and body length of pigs on the date of fat thickness measurement was greater by 4.04 kg and 1.03 cm. Lifetime fat thickness at three measurement points at the level of 6–7 thoracic vertebrae, on the buttocks and in the midpoint of the back between withers and buttocks was thinner by 0.92; 0.94 and 1.06 mm. Evaluation indices of fattening and meat qualities by growth energy and fat thickness (Ia) and average daily gain and fat thickness (Iv) in boars indicate their higher level by 2.07 and 7.67 points compared to pigs and are equal to, respectively, 95.12 ± 1.95 and 152.96 ± 2.38 points. The coefficient of variability (Cv, %) of indicators of own productivity and estimated indices of fattening and meat qualities of repair boars ranged from 1.92% (body length on the date of fat thickness measurement, cm) to 15.12% (age of live weight 100 kg, days), repair pigs – from 4.63% (body length on the date of fat thickness measurement, cm) to 28.19% (index of assessment for growth energy and fat thickness, (Ia), points). The best average values of indicators of breeding value of animals are found on the basis of modern, most promising method BLUP, because it is considered in terms of mathematics, statistics and selection is the most accurate, well-founded, has the best theoretical basis and the lowest error variance. The average value of the BLUP index (parent lines) for boars of large white breed is 101.71 ± 1.681, pigs – 96.94 ± 0.724 points. At the same time, boars outweighed pigs by 4.77 points. The most positive correlation between live weight of piglets at weaning with their body length (r = 0.16), average daily gain (r = 0.19) and fat thickness (r = 0.24), the average correlation between the indices of fattening and meat qualities according to MD Berezovsky and B. Tyler (r = 0.56) P > 0.99 and the weak correlation between live weight of piglets at weaning and the BLUR index (r = 0,21) P > 0.99. It should be noted that there are almost no correlations between the fattening and meat quality indices of pigs and the BLUP index. Conclusions. In the conditions of breeding and specialized farms for the production of commercial pork in the region, to determine the breeding value of animals for further reproduction of their own herd and sale to other breeding farms of Ukraine, to systematically assess repair young animals for their own productivity and clear qualities of pigs and the most perfect BLUP index.


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