scholarly journals The effect of benzothiazolium salt on spruce callus cells

2010 ◽  
Vol 56 (No. 10) ◽  
pp. 463-469 ◽  
Author(s):  
Ľ. Krajnáková ◽  
D. Kákoniová ◽  
D. Lišková ◽  
E. Hlinková

The effect of 3-(benzyloxycarbonylmethyl)-2-benzothiazolinone (SM-550) on the growth parameters, cell division, and cell polymorphism of spruce callus cells (Picea abies L. Karst) was investigated. These results were compared with callus parameters grown on the medium supplemented with NAA. The highest concentration (1 mmol) of SM-550 stimulated the growth process, as well as cell division, shortened the lag-phase, and had a significant effect on cells polymorphism. Its effect was demonstrated especially on long-term culture (3 subcultures – 84 days). On the other hand, the highest stimulation of growth by SM-550 in 1 μmol concentration was determined only in the first subculture. SM-550 in the lowest concentration (1 nmol) used was completely unsuitable in the third subculture, the callus was necrotic and resembled to calli growing on the medium without growth hormones.

1978 ◽  
Vol 88 (2) ◽  
pp. 274-284 ◽  
Author(s):  
Alberto J. Carrillo ◽  
Charles H. Sawyer

ABSTRACT The internal variations (i. e. in timing, duration and amplitude) of the LH surge mechanism within individual rats were examined by monitoring from 3 to 7 successive pro-oestrous LH surges in each of 5 regular 4-day cycling rats fitted with chronic intravenous cannulas. On each successive pro-oestrus blood was collected (0.5–0.6 ml hourly from 14.00–21.00) for radioimmunoassay of LH. The surgery of cannulation had no long-term effect on the regularity of the oestrous cycle. Two rats did, however, show briefly irregular cycles, including one with a 9-day period of anoestrus (pseudopregnancy). In three of the five rats successive pro-oestrous plasma LH curves (4 in one and 3 each in the other two) were internally very consistent in timing, shape and amplitude, However, between each 2 of these 3 animals there were distinct differences in the LH secretory patterns, by as much as 2 h in timing of the onset of the surge and its peak amplitude. The first two surges of the other two rats were atypical of their subsequent surges, which were mostly consistent in timing and amplitude. The pro-oestrous LH surge following the 9-day period of anoestrus was advanced by 2 h and elevated to twice the mean peak amplitude of the cyclic LH surges in that rat. Subsequently, post-PSP surges were studied in rats made pseudopregnant by mechanical stimulation of the cervix. In all cases the immediate post-PSP surge occurred earlier in the afternoon and with a greater peak amplitude than the subsequent cyclic LH surge in the same rat.


1986 ◽  
Vol 67 (6) ◽  
pp. 425-427
Author(s):  
L. A. Kozlov ◽  
I. F. Polyakov ◽  
V. V. Sevastyanov ◽  
E. K. Kazimirov

Prevention of increased blood loss in the postpartum and early postpartum periods can be carried out by influencing both the contractility of the uterus and coagulation in the vessels of the placental site. At present, uterine contractility is enhanced most often by administering appropriate drugs, but the use of electrical stimulation of the uterus seems promising. The requests of obstetric practice require long-term use of electrostimulation with excitation of uterine contractions close to natural. It is also desirable that uterine electrostimulation is performed during the first and second periods of labor with its prospective continuation in the third and early postpartum periods.


1989 ◽  
Vol 66 (3) ◽  
pp. 1421-1429 ◽  
Author(s):  
A. De Troyer ◽  
G. A. Farkas

When the parasternal intercostal in a single interspace is selectively denervated in dogs with diaphragmatic paralysis, it continues to shorten during both quiet and occluded inspiration. In the present studies, we have tested the hypothesis that this passive parasternal inspiratory shortening is due to the action of the other parasternal intercostals. Changes in length of the denervated third right parasternal were measured in eight supine phrenicotomized animals. We found that 1) the inspiratory muscle shortening increased after denervation of the third left parasternal but gradually decreased with denervation of the parasternals situated in the second, fourth, and fifth interspaces; 2) the muscle, however, always continued to shorten during inspiration, even after denervation of all the parasternals; 3) stimulating selectively the third left parasternal caused a muscle lengthening; and 4) bilateral stimulation of the parasternals in the second or the fourth interspace produced a muscle shortening. We conclude that 1) the two parasternals situated in the same interspace on both sides of the sternum are mechanically arranged in series, whereas the parasternals located in adjacent interspaces are mechanically arranged in parallel; and 2) if a denervated parasternal continues to shorten during inspiration, this is in part because of the action of the parasternals in the adjacent interspaces and in part because of other inspiratory muscles of the rib cage, possibly the external intercostals and the levator costae.


PEDIATRICS ◽  
1987 ◽  
Vol 79 (3) ◽  
pp. 394-398
Author(s):  
Beverly Raney ◽  
Audrey Evans ◽  
Linda Granowetter ◽  
Louise Schnaufer ◽  
Antonia Uri ◽  
...  

At The Children’s Hospital of Philadelphia, since 1971, six children 3 months to 17 years of age with fibromatosis have been treated with a combination of vincristine, actinomycin D, and cyclophosphamide (VAC). The first three patients also received radiation therapy (5,500 rads). Locally recurrent tumors developed in four of the children after previous operative removal; the other two had tumors that could not be removed initially. The tumors arose in the neck (three patients), pelvis (two patients), or foot (one patient). In the three patients treated with VAC alone, complete disappearance of tumor was confirmed at second operation in two, and greater than 75% shrinkage on CT scans occurred in the third, all at 4 to 6 months after VAC was started. In two of the three patients who received VAC plus radiation therapy, complete disappearance of tumor occurred at 13 and 16 months; the third had no response. Five of the six patients are free of recurrent fibromatosis at 1, 2, 4, and 11 years after VAC was begun; the sixth has required multiple operations during the last 6 years. We conclude that combination chemotherapy with VAC can produce regression of fibromatosis in some children with recurrent or unresectable lesions. The administration of VAC should be considered for children with fibromatosis in whom operative removal is not feasible, would prove mutilating, or is unlikely to produce long-term control of the disease.


2019 ◽  
Vol 85 (14) ◽  
Author(s):  
Marina Français ◽  
Frédéric Carlin ◽  
Véronique Broussolle ◽  
Christophe Nguyen-Thé

ABSTRACTBacterial adaptation is characterized by a lag phase during which cells do not multiply or modify their physiology to cope with the constraints of their environment. Our aim was to determine a sequence of events during the lag phase of growth at low temperature and pH for threeBacillus cereusstrains. The onsets of expression of two genes, one of which is essential for stress adaptation (cshA, coding for a RNA helicase) and one of which is involved in the transition between lag phase and exponential phase (abrB, coding for a transition regulator), were determined using fluorescent transcriptional reporter systems. Regardless of the stressing conditions and the tested strains, thecshApromoter was active very early, while the biomass increased and always did so before the first cell division. At 12°C and pH 7.0, the onset ofcshApromoter activity occurred at between 3 h and 7 h, while the bacterial counts started to increase at between 12 h and 13 h. At pH 5.0 and at 20°C or 30°C, the onset ofcshApromoter activity occurred before 1 h and earlier than at pH 7.0. In contrast, the onset ofabrBpromoter activity depended on the strain and the stressing conditions. In the ATCC 14579 strain, the onset ofabrBpromoter activity always started at between 30 min and 3 h, before biomass increased and cell division occurred. For the other strains, it took place along with the first cell division at 12°C but did so much later during growth under the other tested conditions.IMPORTANCEThe spore-forming bacteriumB. cereusis a major cause of foodborne outbreaks in Europe. SomeB. cereusstrains can grow at low temperatures and low pH in many processed foods. Modeling of the bacterial lag time is hampered by a lack of knowledge of the timing of events occurring during this phase. In this context, the identification of lag phase markers, not currently available, could be a real advance for the better prediction of lag time duration. Currently, no molecular markers of this phase are available. By determining thatcshAwas always expressed early during the lag phase, we provide a molecular marker of the early adaptation process ofB. cereuscells when exposed to low temperature and pH.


2017 ◽  
Vol 33 (5) ◽  
pp. 328-335 ◽  
Author(s):  
Joyce Maas ◽  
Ger P. J. Keijsers ◽  
Claudia M. Cangliosi ◽  
William van der Veld ◽  
Jorg Tanis-Jacobs ◽  
...  

Abstract. Self-control cognitions arise right before or after someone gives in to an unwanted habit. This paper reports on the development of the 11-item Self-Control Cognition Questionnaire (SCCQ) in a series of three studies. In the first study (N = 308), we found that the SCCQ has a two-factor structure and is reliable. The factors were named “Giving way is rewarding” and “Resistance is impossible.” The construct validity of the SCCQ was assessed in the second study (N = 138). As expected, the SCCQ correlated positively and strongly with the preoccupation with unwanted habits and with the experience of craving, and correlations with one’s tendency to consider the long-term consequences of actions were small. The third study demonstrated that the SCCQ discriminates between patients with habit disorders (N = 63) and controls with non-pathological unwanted habits (N = 106). The SCCQ was sensitive to therapeutic change in two patient samples, one suffering from hair pulling disorder and the other from pathological skin picking. The SCCQ is applicable to unwanted habits in general, both pathological and non-pathological. It is proven to have sound psychometric properties and is suitable for use in practice.


1977 ◽  
Vol 55 (23) ◽  
pp. 2866-2872 ◽  
Author(s):  
Valanne Glooschenko ◽  
John N. A. Lott

Chlordane is an organochlorine soil insecticide whose effects upon aquatic ecosystems, and particularly on algae, are little known. Experimental results indicate that chlordane concentrations ranging from 0.1 to 100 μg/ℓ significantly stimulate cell division of Scenedesmus quadricauda, a common planktonic green alga. Chlordane in concentrations from 0.1 to 50 μg/ℓ is also stimulatory to the growth of Chlamydomonas sp., isolated from soil. However, 100 μg/ℓ was inhibitory to cell division in this species.Significant stimulatory effects on the respiration rates of these two species were observed at all concentrations within 3 to 4 h of chlordane addition. Stimulation of respiration increased with increasing chlordane concentration. Similar stimulatory effects on respiration were produced using 2,4-dinitrophenol (DNP), a known uncoupling agent of oxidative phosphorylation. Long-term inhibition of photosynthetic response of Scenedesmus quadricauda treated with the higher chlordane concentrations was observed over the course of the experiment even though a significant increase in cell numbers over the control was noted. A highly significant chlordane–light interaction was observed on photosynthesis of both S. quadricauda and Chlamydomonas sp.


2014 ◽  
pp. 139-144
Author(s):  
Enikő Vári

The experiments were carried out at the Látókép experimental station of the Centre for Agricultural Sciences of University of Debrecen on chernozem soil in a long term winter wheat experiment. As forecrop rotation, we set up two models: a biculture (wheat and corn) and a triculture (pea, wheat and corn). We applied three levels of nutrients during the fertilization process (control, N50P35K40 and N150P105K120). The third variable studied was irrigation in case of which we tested non-irrigated variables (Ö1) and irrigation variables complemented up to the optimum (Ö3). The effect of pre-crops, irrigation and nutrient-supply levels on some growth-parameters (LAI, LAD), weight of dry matter, just as SPADvalues and yield amounts of winter wheat has been investigated in this experiment. We tried to find out the extent of relationship between the different parameters, and we used the correlation analysis. The correlation analyses have confirmed that all of the investigated parameters had almost in all cases close positive correlation to the yield amount. These results have confirmed that the leaf area, the leaf duration, the SPADvalues, the fertilization and the forecrop have altogether resulted in the production of maximum grain yields.


2005 ◽  
Vol 44 (03) ◽  
pp. 107-117
Author(s):  
R. G. Meyer ◽  
W. Herr ◽  
A. Helisch ◽  
P. Bartenstein ◽  
I. Buchmann

SummaryThe prognosis of patients with acute myeloid leukaemia (AML) has improved considerably by introduction of aggressive consolidation chemotherapy and haematopoietic stem cell transplantation (SCT). Nevertheless, only 20-30% of patients with AML achieve long-term diseasefree survival after SCT. The most common cause of treatment failure is relapse. Additionally, mortality rates are significantly increased by therapy-related causes such as toxicity of chemotherapy and complications of SCT. Including radioimmunotherapies in the treatment of AML and myelodyplastic syndrome (MDS) allows for the achievement of a pronounced antileukaemic effect for the reduction of relapse rates on the one hand. On the other hand, no increase of acute toxicity and later complications should be induced. These effects are important for the primary reduction of tumour cells as well as for the myeloablative conditioning before SCT.This paper provides a systematic and critical review of the currently used radionuclides and immunoconjugates for the treatment of AML and MDS and summarizes the literature on primary tumour cell reductive radioimmunotherapies on the one hand and conditioning radioimmunotherapies before SCT on the other hand.


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