ABSTRACTBacterial adaptation is characterized by a lag phase during which cells do not multiply or modify their physiology to cope with the constraints of their environment. Our aim was to determine a sequence of events during the lag phase of growth at low temperature and pH for threeBacillus cereusstrains. The onsets of expression of two genes, one of which is essential for stress adaptation (cshA, coding for a RNA helicase) and one of which is involved in the transition between lag phase and exponential phase (abrB, coding for a transition regulator), were determined using fluorescent transcriptional reporter systems. Regardless of the stressing conditions and the tested strains, thecshApromoter was active very early, while the biomass increased and always did so before the first cell division. At 12°C and pH 7.0, the onset ofcshApromoter activity occurred at between 3 h and 7 h, while the bacterial counts started to increase at between 12 h and 13 h. At pH 5.0 and at 20°C or 30°C, the onset ofcshApromoter activity occurred before 1 h and earlier than at pH 7.0. In contrast, the onset ofabrBpromoter activity depended on the strain and the stressing conditions. In the ATCC 14579 strain, the onset ofabrBpromoter activity always started at between 30 min and 3 h, before biomass increased and cell division occurred. For the other strains, it took place along with the first cell division at 12°C but did so much later during growth under the other tested conditions.IMPORTANCEThe spore-forming bacteriumB. cereusis a major cause of foodborne outbreaks in Europe. SomeB. cereusstrains can grow at low temperatures and low pH in many processed foods. Modeling of the bacterial lag time is hampered by a lack of knowledge of the timing of events occurring during this phase. In this context, the identification of lag phase markers, not currently available, could be a real advance for the better prediction of lag time duration. Currently, no molecular markers of this phase are available. By determining thatcshAwas always expressed early during the lag phase, we provide a molecular marker of the early adaptation process ofB. cereuscells when exposed to low temperature and pH.