scholarly journals Characterization of two closely linked soybean pgip genes and transcript regulation following pathogen infection and wounding

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 480-482 ◽  
Author(s):  
D. Ovidio R ◽  
S. Roberti ◽  
M. Melaragni ◽  
C. Capodicasa ◽  
L.S. Sella ◽  
...  
Keyword(s):  
Nicotiana Benthamiana ◽  
Plant Cell Wall ◽  
Potato Virus X ◽  
Defense Responses ◽  
Differential Regulation ◽  
Cell Wall Protein ◽  
Plant Defense Responses ◽  
Transcript Regulation ◽  
Polygalacturonase Inhibiting Protein

Polygalacturonase-inhibiting protein (PGIP) is a plant cell wall protein that regulates the action of fungal endopolygalacturonases (PG) favouring the formation of oligogalacturonides active as elicitors of plant defense responses. We have isolated two novel soybean PGIP genes (Gmpgip1 and Gmpgip2) and their recognition specificities against fungal PGs have been tested by expressing them in Nicotiana benthamiana using the Potato virus X (PVX) as vector and in Escherichia coli. In both systems GmPGIP1 and GmPGIP2 accumulate but showed no activity against PGs. Transcript regulation of GmPGIP1 and GmPGIP2 showed that these genes undergo a differential regulation following Sclerotinia sclerotiorum infection and wounding.

scholarly journals Suppression of plant defense responses by extracellular metabolites from Pseudomonas syringae pv. tabaci in Nicotiana benthamiana

2013 ◽  
Vol 13 (1) ◽  
pp. 65 ◽  
Author(s):  
Seonghee Lee ◽  
Dong Sik Yang ◽  
Srinivasa Rao Uppalapati ◽  
Lloyd W Sumner ◽  
Kirankumar S Mysore
Keyword(s):  
Plant Defense ◽  
Pseudomonas Syringae ◽  
Nicotiana Benthamiana ◽  
Defense Responses ◽  
Plant Defense Responses ◽  
Extracellular Metabolites

scholarly journals Acibenzolar-S-Methyl Restricts Infection of Nicotiana benthamiana by Plantago Asiatica Mosaic Virus at Two Distinct Stages

2019 ◽  
Vol 32 (11) ◽  
pp. 1475-1486 ◽  
Author(s):  
Yuki Matsuo ◽  
Fawzia Novianti ◽  
Miki Takehara ◽  
Toshiyuki Fukuhara ◽  
Tsutomu Arie ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Fluorescent Protein ◽  
Potato Virus X ◽  
Defense Responses ◽  
Plant Viruses ◽  
Long Distance ◽  
Plantago Asiatica ◽  
Green Fluorescent ◽  
Long Distance Movement

Plant activators, including acibenzolar-S-methyl (ASM), are chemical compounds that stimulate plant defense responses to pathogens. ASM treatment inhibits infection by a variety of plant viruses, however, the mechanisms of this broad-spectrum and strong effect remain poorly understood. We employed green fluorescent protein (GFP)-expressing viruses and Nicotiana benthamiana plants to identify the infection stages that are restricted by ASM. ASM suppressed infection by three viral species, plantago asiatica mosaic virus (PlAMV), potato virus X (PVX), and turnip mosaic virus (TuMV), in inoculated cells. Furthermore, ASM delayed the long-distance movement of PlAMV and PVX, and the cell-to-cell (short range) movement of TuMV. The ASM-mediated delay of long-distance movement of PlAMV was not due to the suppression of viral accumulation in the inoculated leaves, indicating that ASM restricts PlAMV infection in at least two independent steps. We used Arabidopsis thaliana mutants to show that the ASM-mediated restriction of PlAMV infection requires the NPR1 gene but was independent of the dicer-like genes essential for RNA silencing. Furthermore, experiments using protoplasts showed that ASM treatment inhibited PlAMV replication without cell death. Our approach, using GFP-expressing viruses, will be useful for the analysis of mechanisms underlying plant activator–mediated virus restriction.

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

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