scholarly journals Turnip mosaic virus determinants of virulence for Brassica napus resistance genes

2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. S155-S157
Author(s):  
C.E. Jenner ◽  
F. Sánchez ◽  
K. Tomimura ◽  
K. Ohshima ◽  
F. Ponz ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Infectious Clone ◽  
Turnip Mosaic Virus ◽  
Viral Fitness ◽  
Virulence Determinants ◽  
Similar Strategy ◽  
Napus Line

Dominant resistance genes identified in Brassica napus lines are effective against some, but not all, Turnip mosaic virus<br />(TuMV) isolates. An infectious clone of an isolate (UK 1) was used as the basis of chimeric virus constructions using<br />resistance-breaking mutants and other isolates to identify the virulence determinants for three dominant resistance genes.<br />For the resistance gene TuRB01, the presence of either of two mutations affecting the cylindrical inclusion (CI) protein<br />converted the avirulent UK 1 to a virulent isolate. Acquisition of such mutations had a slight cost to viral fitness in<br />plants lacking the resistance gene. A similar strategy is being used to identify the virulence determinants for two more<br />resistance genes present in another B. napus line.

Genetic mapping of the novel Turnip mosaic virus resistance gene TuRB03 in Brassica napus

2003 ◽  
Vol 107 (7) ◽  
pp. 1169-1173 ◽  
Author(s):  
S. L. Hughes ◽  
P. J. Hunter ◽  
A. G. Sharpe ◽  
M. J. Kearsey ◽  
D. J. Lydiate ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Mapping ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Turnip Mosaic Virus ◽  
The Novel ◽  
Virus Resistance Gene

scholarly journals The Dual Role of the Potyvirus P3 Protein of Turnip mosaic virus as a Symptom and Avirulence Determinant in Brassicas

2003 ◽  
Vol 16 (9) ◽  
pp. 777-784 ◽  
Author(s):  
Carol E. Jenner ◽  
Xiaowu Wang ◽  
Kenta Tomimura ◽  
Kazusato Ohshima ◽  
Fernando Ponz ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Genes ◽  
Plant Virus ◽  
Turnip Mosaic Virus ◽  
Single Amino Acid ◽  
Infection Cycle ◽  
In Planta ◽  
Napus Line ◽  
The Uk

Two isolates of the potyvirus Turnip mosaic virus (TuMV), UK 1 and CDN 1, differ both in their general symptoms on the susceptible propagation host Brassica juncea and in their ability to infect B. napus lines possessing a variety of dominant resistance genes. The isolate CDN 1 produces a more extreme mosaic in infected brassica leaves than UK 1 and is able to overcome the resistance genes TuRB01, TuRB04, and TuRB05. The resistance gene TuRB03, in the B. napus line 22S, is effective against CDN 1 but not UK 1. The nucleic acid sequences of the UK 1 and CDN 1 isolates were 90% identical. The C-terminal half of the P3 protein was identified as being responsible for the differences in symptoms in B. juncea. A single amino acid in the P3 protein was found to be the avirulence determinant for TuRB03. Previous work already has identified the P3 as an avirulence determinant for TuRB04. Our results increase the understanding of the basis of plant-virus recognition, show the importance of the potyviral P3 gene as a symptom determinant, and provide a role in planta for the poorly understood P3 protein in a normal infection cycle.

Evaluation of resistance to Turnip mosaic virus in Australian Brassica napus genotypes

2007 ◽  
Vol 58 (1) ◽  
pp. 67 ◽  
Author(s):  
Brenda A. Coutts ◽  
John A. Walsh ◽  
Roger A. C. Jones
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Resistance Genes ◽  
Systemic Infection ◽  
Turnip Mosaic Virus ◽  
Resistance Response ◽  
Breeding Lines ◽  
Necrotic Spots ◽  
Low Incidence ◽  
Tumv Resistance

Forty-three Australian cultivars or breeding lines of Brassica napus (canola, oilseed rape) and 2 cultivars of Brassica juncea (mustard) were inoculated with infective sap containing isolate WA-Ap of Turnip mosaic virus (TuMV), which belongs to TuMV pathotype 8. The types of reactions obtained were: necrotic spots in inoculated leaves without systemic infection (RN), chlorotic blotches in inoculated leaves without systemic infection (R), and chlorotic blotches in inoculated leaves accompanied by systemic infection that consisted of either necrotic spots (+N) or chlorotic blotches (+). The RN and +N reactions are consistent with those expected in the presence of 4 strain-specific TuMV resistance genes TuRB01 (+N response), TuRB03 (+N response) and TuRB04 with TuRB05 (RN), with + indicating a susceptible response. However, which resistance gene corresponds to the R response is unclear. The RN (TuRB04 with TuRB05) type of response was the commonest. Only one genotype lacked any TuMV resistance, and segregation for more than one different type of resistance response occurred within 22 genotypes and some segregated for resistance and susceptibility. Some genotypes segregated for all 3 types of resistance response found. The reaction of 2 plants of cv. Rivette was atypical as they developed both necrotic spots in inoculated leaves and systemic chlorotic spots. Since breeding for TuMV resistance is not undertaken in Australia, these results indicate frequent but inadvertent crossing with parental lines carrying TuMV resistance. Widespread occurrence of TuMV resistance genes and the possibility that many Australian TuMV isolates may not be well adapted to B. napus may explain the low incidence of this virus found in Australian B. napus crops.

scholarly journals Mutations in Turnip mosaic virus P3 and Cylindrical Inclusion Proteins Are Separately Required to Overcome Two Brassica napus Resistance Genes

Virology ◽  
2002 ◽  
Vol 300 (1) ◽  
pp. 50-59 ◽  
Author(s):  
Carol E. Jenner ◽  
Kenta Tomimura ◽  
Kazusato Ohshima ◽  
Sara L. Hughes ◽  
John A. Walsh
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Resistance Genes ◽  
Turnip Mosaic Virus ◽  
Cylindrical Inclusion

Identification and mapping of a novel dominant resistance gene, TuRB07 to Turnip mosaic virus in Brassica rapa

2013 ◽  
Vol 127 (2) ◽  
pp. 509-519 ◽  
Author(s):  
Mina Jin ◽  
Soo-Seong Lee ◽  
Lin Ke ◽  
Jung Sun Kim ◽  
Mi-Suk Seo ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Brassica Rapa ◽  
Turnip Mosaic Virus

Characterisation of resistance to turnip mosaic virus in oilseed rape (Brassica napus) and genetic mapping of TuRB01

1999 ◽  
Vol 99 (7-8) ◽  
pp. 1149-1154 ◽  
Author(s):  
J. A. Walsh ◽  
A. G. Sharpe ◽  
C. E. Jenner ◽  
D. J. Lydiate
Keyword(s):  
Brassica Napus ◽  
Genetic Mapping ◽  
Mosaic Virus ◽  
Oilseed Rape ◽  
Turnip Mosaic Virus

scholarly journals Gain of Virulence on Rsv1-Genotype Soybean by an Avirulent Soybean mosaic virus Requires Concurrent Mutations in Both P3 and HC-Pro

2008 ◽  
Vol 21 (7) ◽  
pp. 931-936 ◽  
Author(s):  
A. L. Eggenberger ◽  
M. R. Hajimorad ◽  
J. H. Hill
Keyword(s):  
Amino Acids ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Hypersensitive Response ◽  
Soybean Mosaic Virus ◽  
Cytoplasmic Inclusion ◽  
Virulence Determinants ◽  
Extreme Resistance ◽  
Helper Component ◽  
Helper Component Proteinase

In soybean, Rsv1, a single dominant resistance gene, invokes extreme resistance (ER) against most Soybean mosaic virus (SMV) strains, including SMV-N, but not SMV-G7, which provokes a virulent lethal systemic hypersensitive response (LSHR). The elicitor functions of the two viruses provoking Rsv1-mediated ER and LSHR have been mapped to the N-terminal 271 amino acids of P3 from SMV-N and SMV-G7, respectively, which differ by nine residues between the two strains. To identify amino acids of P3 from SMV-N provoking Rsv1-mediated ER, the unique residues of SMV-G7 were substituted with those of SMV-N. Of the mutants tested on Rsv1-genotype soybean, only SMV-G7I788R and SMV-G7T948A lost virulence. However, substitution of amino acids of SMV-N, individually or in combination, with the reciprocal residues from SMV-G7 at these two positions failed to confer virulence to SMV-N. In the search for additional virulence determinants, a series of SMV-N chimeras was generated in which fragments within a region from near the middle of the helper-component proteinase (HC-Pro) cistron to the 5′ end of the cytoplasmic inclusion cistron, nucleotides 1,605 to 3,787, were replaced with those of SMV-G7. Only SMV-N-derived chimeras harboring the 3′ region of HC-Pro, at least from nucleotide 2,013, and the entire 5′ end of P3 (nucleotides 2,430 to 3,237) from SMV-G7 were virulent whereas reciprocal exchanges resulted in loss of SMV-G7 virulence. This region of HC-Pro differs by three amino acids between SMV-N and SMV-G7. Analyses of SMV-G7-derived HC-Pro site-directed mutants showed that only SMV-G7M683R lost virulence on Rsv1-genotype soybean; however, SMV-NR682M failed to gain virulence. Nevertheless, an SMV-N derived mutant with three concurrent substitutions, R682M+R787I+A947T, gained virulence. The data indicate that both P3 and HC-Pro are involved in virulence of SMV on Rsv1-genotype soybean.

scholarly journals Turnip mosaic virus: levantamento, identificação, caracterização biológica e molecular e aspectos epidemiológicos dos isolados brasileiros que infectam brássicas

2019 ◽  
Author(s):  
◽  
Leilane Karam Rodrigues
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
Em 12

O turnip mosaic virus (TuMV) infecta espécies de diferentes famílias botânicas, sendo o único potyvírus capaz de infectar brássicas. Pode ser transmistido mecanicamente e de modo não persistente por mais de 80 espécies de afídeos. O TuMV é classificado em 12 patotipos, de acordo com reações em linhagens de Brassica napus que contêm (ou não) diferentes genes de resistência ao TuMV. Apesar de ser considerado o vírus mais importante que infecta brássicas no mundo, ainda há pouca informação sobre esse vírus na América do Sul. Com o objetivo de avançar no conhecimento da ocorrência, variabilidade genética e de aspectos biológicos e epidemiológicos do TuMV no Brasil, foram identificados e caracterizados 44 isolados de diferentes regiões produtoras de olerícolas. Cinco isolados tiveram seus genomas completamente sequenciados e, de acordo com análises filogenéticas da porção genômica correspondente à capa proteica, agruparam-se nos clados “World-B”, “Basal-B” e “BR”.

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