scholarly journals Mutations in Turnip mosaic virus P3 and Cylindrical Inclusion Proteins Are Separately Required to Overcome Two Brassica napus Resistance Genes

Virology ◽  
2002 ◽  
Vol 300 (1) ◽  
pp. 50-59 ◽  
Author(s):  
Carol E. Jenner ◽  
Kenta Tomimura ◽  
Kazusato Ohshima ◽  
Sara L. Hughes ◽  
John A. Walsh
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Resistance Genes ◽  
Turnip Mosaic Virus ◽  
Cylindrical Inclusion

scholarly journals Turnip mosaic virus determinants of virulence for Brassica napus resistance genes

2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. S155-S157
Author(s):  
C.E. Jenner ◽  
F. Sánchez ◽  
K. Tomimura ◽  
K. Ohshima ◽  
F. Ponz ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Infectious Clone ◽  
Turnip Mosaic Virus ◽  
Viral Fitness ◽  
Virulence Determinants ◽  
Similar Strategy ◽  
Napus Line

Dominant resistance genes identified in Brassica napus lines are effective against some, but not all, Turnip mosaic virus<br />(TuMV) isolates. An infectious clone of an isolate (UK 1) was used as the basis of chimeric virus constructions using<br />resistance-breaking mutants and other isolates to identify the virulence determinants for three dominant resistance genes.<br />For the resistance gene TuRB01, the presence of either of two mutations affecting the cylindrical inclusion (CI) protein<br />converted the avirulent UK 1 to a virulent isolate. Acquisition of such mutations had a slight cost to viral fitness in<br />plants lacking the resistance gene. A similar strategy is being used to identify the virulence determinants for two more<br />resistance genes present in another B. napus line.

Evaluation of resistance to Turnip mosaic virus in Australian Brassica napus genotypes

2007 ◽  
Vol 58 (1) ◽  
pp. 67 ◽  
Author(s):  
Brenda A. Coutts ◽  
John A. Walsh ◽  
Roger A. C. Jones
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Resistance Genes ◽  
Systemic Infection ◽  
Turnip Mosaic Virus ◽  
Resistance Response ◽  
Breeding Lines ◽  
Necrotic Spots ◽  
Low Incidence ◽  
Tumv Resistance

Forty-three Australian cultivars or breeding lines of Brassica napus (canola, oilseed rape) and 2 cultivars of Brassica juncea (mustard) were inoculated with infective sap containing isolate WA-Ap of Turnip mosaic virus (TuMV), which belongs to TuMV pathotype 8. The types of reactions obtained were: necrotic spots in inoculated leaves without systemic infection (RN), chlorotic blotches in inoculated leaves without systemic infection (R), and chlorotic blotches in inoculated leaves accompanied by systemic infection that consisted of either necrotic spots (+N) or chlorotic blotches (+). The RN and +N reactions are consistent with those expected in the presence of 4 strain-specific TuMV resistance genes TuRB01 (+N response), TuRB03 (+N response) and TuRB04 with TuRB05 (RN), with + indicating a susceptible response. However, which resistance gene corresponds to the R response is unclear. The RN (TuRB04 with TuRB05) type of response was the commonest. Only one genotype lacked any TuMV resistance, and segregation for more than one different type of resistance response occurred within 22 genotypes and some segregated for resistance and susceptibility. Some genotypes segregated for all 3 types of resistance response found. The reaction of 2 plants of cv. Rivette was atypical as they developed both necrotic spots in inoculated leaves and systemic chlorotic spots. Since breeding for TuMV resistance is not undertaken in Australia, these results indicate frequent but inadvertent crossing with parental lines carrying TuMV resistance. Widespread occurrence of TuMV resistance genes and the possibility that many Australian TuMV isolates may not be well adapted to B. napus may explain the low incidence of this virus found in Australian B. napus crops.

Genetic mapping of the novel Turnip mosaic virus resistance gene TuRB03 in Brassica napus

2003 ◽  
Vol 107 (7) ◽  
pp. 1169-1173 ◽  
Author(s):  
S. L. Hughes ◽  
P. J. Hunter ◽  
A. G. Sharpe ◽  
M. J. Kearsey ◽  
D. J. Lydiate ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Mapping ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Turnip Mosaic Virus ◽  
The Novel ◽  
Virus Resistance Gene

scholarly journals The Dual Role of the Potyvirus P3 Protein of Turnip mosaic virus as a Symptom and Avirulence Determinant in Brassicas

2003 ◽  
Vol 16 (9) ◽  
pp. 777-784 ◽  
Author(s):  
Carol E. Jenner ◽  
Xiaowu Wang ◽  
Kenta Tomimura ◽  
Kazusato Ohshima ◽  
Fernando Ponz ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Genes ◽  
Plant Virus ◽  
Turnip Mosaic Virus ◽  
Single Amino Acid ◽  
Infection Cycle ◽  
In Planta ◽  
Napus Line ◽  
The Uk

Two isolates of the potyvirus Turnip mosaic virus (TuMV), UK 1 and CDN 1, differ both in their general symptoms on the susceptible propagation host Brassica juncea and in their ability to infect B. napus lines possessing a variety of dominant resistance genes. The isolate CDN 1 produces a more extreme mosaic in infected brassica leaves than UK 1 and is able to overcome the resistance genes TuRB01, TuRB04, and TuRB05. The resistance gene TuRB03, in the B. napus line 22S, is effective against CDN 1 but not UK 1. The nucleic acid sequences of the UK 1 and CDN 1 isolates were 90% identical. The C-terminal half of the P3 protein was identified as being responsible for the differences in symptoms in B. juncea. A single amino acid in the P3 protein was found to be the avirulence determinant for TuRB03. Previous work already has identified the P3 as an avirulence determinant for TuRB04. Our results increase the understanding of the basis of plant-virus recognition, show the importance of the potyviral P3 gene as a symptom determinant, and provide a role in planta for the poorly understood P3 protein in a normal infection cycle.

Characterisation of resistance to turnip mosaic virus in oilseed rape (Brassica napus) and genetic mapping of TuRB01

1999 ◽  
Vol 99 (7-8) ◽  
pp. 1149-1154 ◽  
Author(s):  
J. A. Walsh ◽  
A. G. Sharpe ◽  
C. E. Jenner ◽  
D. J. Lydiate
Keyword(s):  
Brassica Napus ◽  
Genetic Mapping ◽  
Mosaic Virus ◽  
Oilseed Rape ◽  
Turnip Mosaic Virus

scholarly journals Turnip mosaic virus: levantamento, identificação, caracterização biológica e molecular e aspectos epidemiológicos dos isolados brasileiros que infectam brássicas

2019 ◽  
Author(s):  
◽  
Leilane Karam Rodrigues
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
Em 12

O turnip mosaic virus (TuMV) infecta espécies de diferentes famílias botânicas, sendo o único potyvírus capaz de infectar brássicas. Pode ser transmistido mecanicamente e de modo não persistente por mais de 80 espécies de afídeos. O TuMV é classificado em 12 patotipos, de acordo com reações em linhagens de Brassica napus que contêm (ou não) diferentes genes de resistência ao TuMV. Apesar de ser considerado o vírus mais importante que infecta brássicas no mundo, ainda há pouca informação sobre esse vírus na América do Sul. Com o objetivo de avançar no conhecimento da ocorrência, variabilidade genética e de aspectos biológicos e epidemiológicos do TuMV no Brasil, foram identificados e caracterizados 44 isolados de diferentes regiões produtoras de olerícolas. Cinco isolados tiveram seus genomas completamente sequenciados e, de acordo com análises filogenéticas da porção genômica correspondente à capa proteica, agruparam-se nos clados “World-B”, “Basal-B” e “BR”.

THE DEVELOPMENT OF Turnip mosaic virus RESISTANT DOUBLED HAPLOID LINES OF Brassica napus L.

2013 ◽  
pp. 122-125
Author(s):  
I.А. Zubareva ◽  
◽  
E.N. Goloveshkina ◽  
S.V. Vinogradova ◽  
T.N. Gribova ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Mosaic Virus ◽  
Doubled Haploid ◽  
Turnip Mosaic Virus ◽  
Brassica Napus L ◽  
Doubled Haploid Lines

scholarly journals Resistance Phenotypes in Diverse Accessions, Breeding Lines, and Cultivars of Three Mustard Species Inoculated with Turnip mosaic virus

Plant Disease ◽  
2010 ◽  
Vol 94 (11) ◽  
pp. 1290-1298 ◽  
Author(s):  
Monica A. Kehoe ◽  
Brenda A. Coutts ◽  
Roger A. C. Jones
Keyword(s):  
Mosaic Virus ◽  
Resistance Genes ◽  
Indian Mustard ◽  
Systemic Infection ◽  
Turnip Mosaic Virus ◽  
Biofuel Production ◽  
Systemic Necrosis ◽  
Breeding Lines ◽  
B Genome ◽  
A Genome

The responses of 44 accessions, breeding lines, or cultivars of Brassica juncea (Indian mustard), 9 of B. carinata (Ethiopian mustard), 5 of B. nigra (black mustard), and 6 crosses between B. juncea and B. napus (canola) to sap inoculation with Turnip mosaic virus (TuMV) were investigated. Eleven different phenotypes were obtained, including six previously recognized in B. napus (+, O, R, RN, RN/+, and +N) and five not recorded before (+St, RN/St, RN/St/+, +N1, and +ND). All but two (+ and +St) were resistance phenotypes. The resistance phenotypes in B. carinata and B. juncea × B. napus crosses prevented systemic infection but those in B. juncea and B. nigra included systemic necrosis. Absence of systemic invasion associated with resistance phenotypes in B. carinata was confirmed by graft inoculations. The resistance phenotypes may reflect the presence of known TuMV resistance genes located in the A genome or unknown genes in the B genome in B. juncea, unknown resistance genes in the B or C genomes in B. carinata, and unknown resistance genes in the B genome in B. nigra. Further research to identify the resistance genes involved would establish the potential usefulness of these resistance phenotypes in breeding TuMV-resistant mustard cultivars for biofuel production.

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